To understand the effect of nitrogen(N) fertilizer on rice(Oryza sativa L.) eating and cooking quality(ECQ). Here, we investigated the ECQ attributes, physicochemical foundation of ECQ, and amylopectin fine structure ...To understand the effect of nitrogen(N) fertilizer on rice(Oryza sativa L.) eating and cooking quality(ECQ). Here, we investigated the ECQ attributes, physicochemical foundation of ECQ, and amylopectin fine structure of two Waxy(Wx) alleles japonica rice cultivars Nanjing 9108(NJ9108) and Huaidao 5(HD5) under four N rates(0, 150, 300, and 450 kg ha^-1). Sensory and pasting properties of the two cultivars varied depending on N rates. Compared with the control(0 kg ha^-1), the overall eating quality and sensory value were significantly decreased under the N rates of 300 and 450 kg ha^-1. Further, conventional descriptive analysis showed that the stickiness and retrogradation of cooked rice were significantly decreased. These results indicated that application of N fertilizer seems to affect the texture of cooked rice, causing it to be less sticky, lowering its retrogradation, and consequently reducing its palatability. Results from rapid visco analyzer(RVA) revealed that the peak and breakdown viscosities significantly decreased, while the setback viscosity and peak time increased under the N rates of 300 and 450 kg ha^-1. However, no significant difference was observed when the N rate was 150 kg ha^-1, indicating that less N fertilization can maintain rice ECQ. As the N rate increasing, protein content increased, whereas apparent amylose content, starch content, and gel consistency almost unchanged. Interestingly, compared with the control, under N treatments, the percentage of short amylopectin branches in NJ9108 was decreased, but increased in HD5, as controlled by amylopectin synthesis-related genes. Notably, SSI and BEIIb were down-regulated in NJ9108, whereas BEIIb was up-regulated in HD5. Thus, the palatability of both rice cultivars was significantly decreased under excessive N fertilization as a consequence of reduced stickiness and retrogradation of the cooked rice, which might have resulted from an elevated protein content and altered amylopectin fine structure. In addition, amylopectin synthesis appeared to be affected by N fertilizer and the genotype of the rice cultivar.展开更多
Keeping in view the importance of amylose for various food industries as well as its importance on domestic level, the current study was conducted to analyze the amylose content of pulses of common use e.g. mungbean ...Keeping in view the importance of amylose for various food industries as well as its importance on domestic level, the current study was conducted to analyze the amylose content of pulses of common use e.g. mungbean (NM-92, NM-98 and Ramazan) and kabuli type chickpea (CMNKI0-99, CMNK452-2 and Hassan 2k) varieties and quantifying the influence of cooking (boiling) on the amylose contents of pulses. The data revealed that moisture and arnylose content of uncooked mungbean varieties ranged 9.01-9.47% and 20.06-22.26% respectively. The moisture and amylose content of Kabuli type chickpea varieties varied 8.52-8.79% and 20.25-22.83% respectively. Moisture content of mungbean (64.16-66.08%) as well as chickpea (55.56-61.52%) varieties increased after cooking. For all the three varieties of mungbean the observed cooking time was 11 minutes. The maximum value of amylose content was assayed for NM-98 (20.74%) followed by Ramzan (20.24%). The cooking time of Kabuli type chickpea varieties CMNK-452-2 and CMNK-10-99 was 60 min. Hassan 2K observed more cooking time (90 min) and highest value of amylose content in cooked (19.01%) samples. The effect of cooking/varietals on sensory evaluation (appearance, flavor, texture and overall acceptability) of mungbean varieties was statistically non significant attributes. Among chickpea varieties, Hassan 2K was ranked lowest due to its small size and hard texture and the sensory attributes were statistically significant (P 〈 0.05) as compared to the other two varieties.展开更多
基金funded by the China Postdoctoral Science Foundation(2017M611753)the earmarked fund for Agriculture Research System of China(CARS-01-62)+3 种基金the National Key R&D Program of China(2015BAD01B02)the Natural Science Foundation of Jiangsu Province,China(BK20180302)the Jiangsu Agriculture Science and Technology Innovation Fund,China(CX[17]3009)the Project of Henan Key Laboratory of Tea Plant Comprehensive Utilization in South Henan,China(HNKLTCU2018004)。
文摘To understand the effect of nitrogen(N) fertilizer on rice(Oryza sativa L.) eating and cooking quality(ECQ). Here, we investigated the ECQ attributes, physicochemical foundation of ECQ, and amylopectin fine structure of two Waxy(Wx) alleles japonica rice cultivars Nanjing 9108(NJ9108) and Huaidao 5(HD5) under four N rates(0, 150, 300, and 450 kg ha^-1). Sensory and pasting properties of the two cultivars varied depending on N rates. Compared with the control(0 kg ha^-1), the overall eating quality and sensory value were significantly decreased under the N rates of 300 and 450 kg ha^-1. Further, conventional descriptive analysis showed that the stickiness and retrogradation of cooked rice were significantly decreased. These results indicated that application of N fertilizer seems to affect the texture of cooked rice, causing it to be less sticky, lowering its retrogradation, and consequently reducing its palatability. Results from rapid visco analyzer(RVA) revealed that the peak and breakdown viscosities significantly decreased, while the setback viscosity and peak time increased under the N rates of 300 and 450 kg ha^-1. However, no significant difference was observed when the N rate was 150 kg ha^-1, indicating that less N fertilization can maintain rice ECQ. As the N rate increasing, protein content increased, whereas apparent amylose content, starch content, and gel consistency almost unchanged. Interestingly, compared with the control, under N treatments, the percentage of short amylopectin branches in NJ9108 was decreased, but increased in HD5, as controlled by amylopectin synthesis-related genes. Notably, SSI and BEIIb were down-regulated in NJ9108, whereas BEIIb was up-regulated in HD5. Thus, the palatability of both rice cultivars was significantly decreased under excessive N fertilization as a consequence of reduced stickiness and retrogradation of the cooked rice, which might have resulted from an elevated protein content and altered amylopectin fine structure. In addition, amylopectin synthesis appeared to be affected by N fertilizer and the genotype of the rice cultivar.
文摘Keeping in view the importance of amylose for various food industries as well as its importance on domestic level, the current study was conducted to analyze the amylose content of pulses of common use e.g. mungbean (NM-92, NM-98 and Ramazan) and kabuli type chickpea (CMNKI0-99, CMNK452-2 and Hassan 2k) varieties and quantifying the influence of cooking (boiling) on the amylose contents of pulses. The data revealed that moisture and arnylose content of uncooked mungbean varieties ranged 9.01-9.47% and 20.06-22.26% respectively. The moisture and amylose content of Kabuli type chickpea varieties varied 8.52-8.79% and 20.25-22.83% respectively. Moisture content of mungbean (64.16-66.08%) as well as chickpea (55.56-61.52%) varieties increased after cooking. For all the three varieties of mungbean the observed cooking time was 11 minutes. The maximum value of amylose content was assayed for NM-98 (20.74%) followed by Ramzan (20.24%). The cooking time of Kabuli type chickpea varieties CMNK-452-2 and CMNK-10-99 was 60 min. Hassan 2K observed more cooking time (90 min) and highest value of amylose content in cooked (19.01%) samples. The effect of cooking/varietals on sensory evaluation (appearance, flavor, texture and overall acceptability) of mungbean varieties was statistically non significant attributes. Among chickpea varieties, Hassan 2K was ranked lowest due to its small size and hard texture and the sensory attributes were statistically significant (P 〈 0.05) as compared to the other two varieties.