As low-cost and highly autonomous ocean observation platforms,underwater gliders encounter risks during their launch and recovery,especially when coordinating multi-glider deployments.This work focuses on cooperative ...As low-cost and highly autonomous ocean observation platforms,underwater gliders encounter risks during their launch and recovery,especially when coordinating multi-glider deployments.This work focuses on cooperative path planning of an underwater glider fleet with simultaneous launch and recovery to enhance the autonomy of sampling and reduce deployment risks.Specifically,the gliders collaborate to achieve sampling considering the specified routines of interest.The overall paths to be planned are divided into four rectangular parts with the same starting point,and each glider is assigned a local sampling route.A clipped-oriented line-of-sight algorithm is proposed to ensure the coverage of the desired edges.The pitch angle of the glider is selected as the optimizing parameter to coordinate the overall progress considering the susceptibility of gliders to currents and the randomness of paths produced by complex navigational strategies.Therefore,a multi-actuation deep-Q network algorithm is proposed to ensure simultaneous launch and recovery.Simulation results demonstrate the acceptable effectiveness of the proposed method.展开更多
Providing reliable multicast service is very challenging in Ad Hoc networks. In this paper, we propose an efficient loss recovery scheme for reliable multicast (CoreRM). Our basic idea is to apply the notion of cooper...Providing reliable multicast service is very challenging in Ad Hoc networks. In this paper, we propose an efficient loss recovery scheme for reliable multicast (CoreRM). Our basic idea is to apply the notion of cooperative communications to support local loss recovery in multicast. A receiver node experiencing a packet loss tries to recover the lost packet through progressively cooperating with neighboring nodes, upstream nodes or even source node. In order to reduce recovery latency and retransmission overhead, CoreRM caches not only data packets but also the path which could be used for future possible use to expedite the loss recovery process. Both analytical and simulation results reveal that CoreRM significantly improves the reliable multicast performance in terms of delivery ratio, throughput and recovery latency compared with UDP and PGM.展开更多
Intersections and discontinuities commonly arise in surface modeling and cause problems in downstream operations. Local geometry repair, such as cover holes or replace bad surfaces by adding new surface patches for de...Intersections and discontinuities commonly arise in surface modeling and cause problems in downstream operations. Local geometry repair, such as cover holes or replace bad surfaces by adding new surface patches for dealing with inconsistencies among the confluent region, where multiple surfaces meet, is a common technique used in CAD model repair and reverse engineering. However, local geometry repair destroys the topology of original CAD model and increases the number of surface patches needed for freeform surface shape modeling. Consequently, a topology recovery technique dealing with complex freeform surface model after local geometry repair is proposed. Firstly, construct the curve network which freeform surface model; secondly, apply freeform surface fitting method determine the geometry and topology properties of recovery to create B-spline surface patches to recover the topology of trimmed ones. Corresponding to the two levels of enforcing boundary conditions on a B-spline surface, two solution schemes are presented respectively. In the first solution scheme, non-constrained B-spline surface fitting method is utilized to piecewise recover trimmed confluent surface patches and then employs global beautification technique to smoothly stitch the recovery surface patches. In the other solution scheme, constrained B-spline surface fitting technique based on discretization of boundary conditions is directly applied to recover topology of surface model after local geometry repair while achieving G~ continuity simultaneously. The presented two different schemes are applied to the consistent surface model, which consists of five trimmed confluent surface patches and a local consistent surface patch, and a machine cover model, respectively. The application results show that our topology recovery technique meets shape-preserving and Gt continuity requirements in reverse engineering. This research converts the problem of topology recovery for consistent surface model to the problem of constructing G1 patches from a given curve network, and provides a new idea to model repairing study.展开更多
Spinal cord injury often leads to severe motor and sensory deficits,and prognosis using the currently available therapies remains poor.Therefore,we aimed to explore a novel therapeutic approach for improving the progn...Spinal cord injury often leads to severe motor and sensory deficits,and prognosis using the currently available therapies remains poor.Therefore,we aimed to explore a novel therapeutic approach for improving the prognosis of spinal cord injury.In this study,we implanted oscillating field stimulation devices and transplanted neural stem cells into the thoracic region(T9–T10)of rats with a spinal cord contusion.Basso-Beattie-Bresnahan scoring revealed that oscillating field stimulation combined with neural stem cells transplantation promoted motor function recovery following spinal cord injury.In addition,we investigated the regulation of oscillating field stimulation on the miR-124/Tal1 axis in neural stem cells.Transfection of lentivirus was performed to investigate the role of Tal1 in neurogenesis of neural stem cells induced by oscillating field stimulation.Quantitative reverse transcription-polymerase chain reaction,immunofluorescence and western blotting showed that oscillating field stimulation promoted neurogenesis of neural stem cells in vitro and in vivo.Hematoxylin and eosin staining showed that oscillating field stimulation combined with neural stem cells transplantation alleviated cavities formation after spinal cord injury.Taking the results together,we concluded that oscillating field stimulation decreased miR-124 expression and increased Tal1 content,thereby promoting the neurogenesis of neural stem cells.The combination of oscillating field stimulation and neural stem cells transplantation improved neurogenesis,and thereby promoted structural and functional recovery after spinal cord injury.展开更多
Chronic denervation is one of the key factors that affect nerve regeneration.Chronic axotomy deteriorates the distal nerve stump,causes protein changes,and renders the microenvironment less permissive for regeneration...Chronic denervation is one of the key factors that affect nerve regeneration.Chronic axotomy deteriorates the distal nerve stump,causes protein changes,and renders the microenvironment less permissive for regeneration.Some of these factors/proteins have been individually studied.To better delineate the comprehensive protein expression profiles and identify proteins that contribute to or are associated with this detrimental effect,we carried out a proteomic analysis of the distal nerve using an established delayed rat sciatic nerve repair model.Four rats that received immediate repair after sciatic nerve transection served as control,whereas four rats in the experimental group(chronic denervation)had their sciatic nerve repaired after a 12-week delay.All the rats were sacrificed after 16 weeks to harvest the distal nerves for extracting proteins.Twenty-five micrograms of protein from each sample were fractionated in SDS-PAGE gels.NanoLC-MS/MS analysis was applied to the gels.Protein expression levels of nerves on the surgery side were compared to those on the contralateral side.Any protein with a P value of less than 0.05 and a fold change of 4 or higher was deemed differentially expressed.All the differentially expressed proteins in both groups were further stratified according to the biological processes.A PubMed search was also conducted to identify the differentially expressed proteins that have been reported to be either beneficial or detrimental to nerve regeneration.Ingenuity Pathway Analysis(IPA)software was used for pathway analysis.The results showed that 709 differentially expressed proteins were identified in the delayed repair group,with a bigger proportion of immune and inflammatory process-related proteins and a smaller proportion of proteins related to axon regeneration and lipid metabolism in comparison to the control group where 478 differentially expressed proteins were identified.The experimental group also had more beneficial proteins that were downregulated and more detrimental proteins that were upregulated.IPA revealed that protective pathways such as LXR/RXR,acute phase response,RAC,ERK/MAPK,CNTF,IL-6,and FGF signaling were inhibited in the delayed repair group,whereas three detrimental pathways,including the complement system,PTEN,and apoptosis signaling,were activated.An available database of the adult rodent sciatic nerve was used to assign protein changes to specific cell types.The poor regeneration seen in the delayed repair group could be associated with the down-regulation of beneficial proteins and up-regulation of detrimental proteins.The proteins and pathways identified in this study may offer clues for future studies to identify therapeutic targets.展开更多
随着纠删码在分布式存储系统中的实际应用,纠删码为存储系统提供了更加优秀的存储效率,但当节点丢失时,相较于传统副本技术更多的网络传输带宽开销成为了造成系统性能瓶颈的关键因素。为了解决MDS编码高带宽开销对系统性能的影响,一类...随着纠删码在分布式存储系统中的实际应用,纠删码为存储系统提供了更加优秀的存储效率,但当节点丢失时,相较于传统副本技术更多的网络传输带宽开销成为了造成系统性能瓶颈的关键因素。为了解决MDS编码高带宽开销对系统性能的影响,一类新型编码方案——分组码被应用在分布式存储系统中,相较于传统MDS编码能够有效地降低节点修复时的数据传输量,从而减少网络带宽需求。在Pyramid分组码的基础上进行层次扩展,提出一种HLRC(hierarchical local repair codes)纠删码。HLRC相较于LRC引入了层次编码模型,将原始数据块构建为编码矩阵,根据层次进行分别编码,生成包含数据块范围不同的局部校验块;每个层次包含的数据块数量不同,可以保证修复节点时的低修复成本,同时还拥有较高的存储效率。HLRC相较于Pyramid拥有额外的校验块冗余,能够降低校验块出错和多节点出错时的恢复开销。在基于Ceph的分布式存储系统中的实验结果表明,HLRC与Pyramid等分组码相比,单节点修复开销最高可降低48.56%,多节点修复开销最高可降低25%。展开更多
连线干涉测量(Connected Element Interferometry,CEI)是一种全天时全天候的被动测角技术,已用于空间目标的跟踪监视.地球静止轨道(Geostationary Earth Orbit,GEO)卫星需要频繁机动以保持轨位或完成其他任务,其机动后的快速轨道恢复能...连线干涉测量(Connected Element Interferometry,CEI)是一种全天时全天候的被动测角技术,已用于空间目标的跟踪监视.地球静止轨道(Geostationary Earth Orbit,GEO)卫星需要频繁机动以保持轨位或完成其他任务,其机动后的快速轨道恢复能力对于监视预警极为重要.针对基于CEI的GEO短弧定轨和预报,分析了定轨算法的形亏和数亏,在附加先验轨道约束的短弧定轨基础上,提出了轨道半长轴初值的自适应优化方法.利用亚太七号卫星的CEI仿真和实测数据进行了短弧定轨和预报,实验结果表明,采用优化后的半长轴初值,30min短弧定轨和10min预报的卫星位置分量精度均优于4km,能够满足非合作GEO目标机动后快速轨道恢复的需求.展开更多
快速可行的灾后恢复是弹性电力系统的重要一环。提出一种弹性导向的考虑修复不确定性和V2G(vehicle to grid,V2G)的城市电力系统动态供电恢复方法,灾后短期内应用V2G站中的有限电动汽车资源为城市电网提供电能支撑,同时派遣抢修队对故...快速可行的灾后恢复是弹性电力系统的重要一环。提出一种弹性导向的考虑修复不确定性和V2G(vehicle to grid,V2G)的城市电力系统动态供电恢复方法,灾后短期内应用V2G站中的有限电动汽车资源为城市电网提供电能支撑,同时派遣抢修队对故障线路进行抢修作业,实现系统快速恢复。对V2G和电动汽车灾前、灾后行为进行建模,通过灾前自由调度统计各V2G站附近的电动汽车数量,按照响应意愿比例得到灾后阶段各V2G站反向输电功率;对包含行驶行为和修复行为的动态修复行为进行建模,采用滚动时域优化方法决策修复的先后顺序,得到抢修队最优动态修复方案。最后,设计多组算例以验证所提方法的可行性,结果表明,该方法可有效支撑灾后城市电力系统快速恢复,提高系统弹性。展开更多
Peripheral nerve injuries induce a severe motor and sensory deficit. Since the availability of autologous nerve transplants for nerve repair is very limited, alternative treatment strategies are sought, including the ...Peripheral nerve injuries induce a severe motor and sensory deficit. Since the availability of autologous nerve transplants for nerve repair is very limited, alternative treatment strategies are sought, including the use of tubular nerve guidance conduits(tNGCs). However, the use of tNGCs results in poor functional recovery and central necrosis of the regenerating tissue, which limits their application to short nerve lesion defects(typically shorter than 3 cm). Given the importance of vascularization in nerve regeneration, we hypothesized that enabling the growth of blood vessels from the surrounding tissue into the regenerating nerve within the tNGC would help eliminate necrotic processes and lead to improved regeneration. In this study, we reported the application of macroscopic holes into the tubular walls of silk-based tNGCs and compared the various features of these improved silk^(+) tNGCs with the tubes without holes(silk^(–) tNGCs) and autologous nerve transplants in an 8-mm sciatic nerve defect in rats. Using a combination of micro-computed tomography and histological analyses, we were able to prove that the use of silk^(+) tNGCs induced the growth of blood vessels from the adjacent tissue to the intraluminal neovascular formation. A significantly higher number of blood vessels in the silk^(+) group was found compared with autologous nerve transplants and silk^(–), accompanied by improved axon regeneration at the distal coaptation point compared with the silk^(–) tNGCs at 7 weeks postoperatively. In the 15-mm(critical size) sciatic nerve defect model, we again observed a distinct ingrowth of blood vessels through the tubular walls of silk^(+) tNGCs, but without improved functional recovery at 12 weeks postoperatively. Our data proves that macroporous tNGCs increase the vascular supply of regenerating nerves and facilitate improved axonal regeneration in a short-defect model but not in a critical-size defect model. This study suggests that further optimization of the macroscopic holes silk^(+) tNGC approach containing macroscopic holes might result in improved grafting technology suitable for future clinical use.展开更多
为了减弱极端事件对综合能源系统的影响,尽早恢复各类负荷,提高系统弹性。文中提出了一种考虑交通网与配电网级别的区域电-气互联系统(Interconnection Electric and Gas System, IEGS)协同运行的灾后故障抢修策略。首先,通过元胞传输...为了减弱极端事件对综合能源系统的影响,尽早恢复各类负荷,提高系统弹性。文中提出了一种考虑交通网与配电网级别的区域电-气互联系统(Interconnection Electric and Gas System, IEGS)协同运行的灾后故障抢修策略。首先,通过元胞传输模型建立了考虑系统实时恢复指标修正的交通流量分配模型,对交通网的交通流量进行预测;其次,在故障预分配的前提下建立了区域IEGS的故障恢复模型,协调优化维修队、分布式电源、配电网网架等资源,减少停电损失;然后以维修队的最小通行时间矩阵以及由系统实时恢复指标得到的交通网道路修正参数作为传递变量,在维修队开始移动的时间点,重新计算后续的修复计划以及交通网的运行情况,进行多时间断面优化;最后,通过算例仿真进行对比,验证了所提策略的有效性,并分析移动储能装置对故障恢复的影响。展开更多
基金supported by the National Natural Science Foundation of China(No.51909252)the Fundamental Research Funds for the Central Universities(No.202061004)This work is also partly supported by the China Scholar Council.
文摘As low-cost and highly autonomous ocean observation platforms,underwater gliders encounter risks during their launch and recovery,especially when coordinating multi-glider deployments.This work focuses on cooperative path planning of an underwater glider fleet with simultaneous launch and recovery to enhance the autonomy of sampling and reduce deployment risks.Specifically,the gliders collaborate to achieve sampling considering the specified routines of interest.The overall paths to be planned are divided into four rectangular parts with the same starting point,and each glider is assigned a local sampling route.A clipped-oriented line-of-sight algorithm is proposed to ensure the coverage of the desired edges.The pitch angle of the glider is selected as the optimizing parameter to coordinate the overall progress considering the susceptibility of gliders to currents and the randomness of paths produced by complex navigational strategies.Therefore,a multi-actuation deep-Q network algorithm is proposed to ensure simultaneous launch and recovery.Simulation results demonstrate the acceptable effectiveness of the proposed method.
文摘Providing reliable multicast service is very challenging in Ad Hoc networks. In this paper, we propose an efficient loss recovery scheme for reliable multicast (CoreRM). Our basic idea is to apply the notion of cooperative communications to support local loss recovery in multicast. A receiver node experiencing a packet loss tries to recover the lost packet through progressively cooperating with neighboring nodes, upstream nodes or even source node. In order to reduce recovery latency and retransmission overhead, CoreRM caches not only data packets but also the path which could be used for future possible use to expedite the loss recovery process. Both analytical and simulation results reveal that CoreRM significantly improves the reliable multicast performance in terms of delivery ratio, throughput and recovery latency compared with UDP and PGM.
基金supported by China Postdoctoral Science Foundation(Grant No. 20110490376)National Natural Science Foundation of China (Grant No. 50575098)
文摘Intersections and discontinuities commonly arise in surface modeling and cause problems in downstream operations. Local geometry repair, such as cover holes or replace bad surfaces by adding new surface patches for dealing with inconsistencies among the confluent region, where multiple surfaces meet, is a common technique used in CAD model repair and reverse engineering. However, local geometry repair destroys the topology of original CAD model and increases the number of surface patches needed for freeform surface shape modeling. Consequently, a topology recovery technique dealing with complex freeform surface model after local geometry repair is proposed. Firstly, construct the curve network which freeform surface model; secondly, apply freeform surface fitting method determine the geometry and topology properties of recovery to create B-spline surface patches to recover the topology of trimmed ones. Corresponding to the two levels of enforcing boundary conditions on a B-spline surface, two solution schemes are presented respectively. In the first solution scheme, non-constrained B-spline surface fitting method is utilized to piecewise recover trimmed confluent surface patches and then employs global beautification technique to smoothly stitch the recovery surface patches. In the other solution scheme, constrained B-spline surface fitting technique based on discretization of boundary conditions is directly applied to recover topology of surface model after local geometry repair while achieving G~ continuity simultaneously. The presented two different schemes are applied to the consistent surface model, which consists of five trimmed confluent surface patches and a local consistent surface patch, and a machine cover model, respectively. The application results show that our topology recovery technique meets shape-preserving and Gt continuity requirements in reverse engineering. This research converts the problem of topology recovery for consistent surface model to the problem of constructing G1 patches from a given curve network, and provides a new idea to model repairing study.
基金supported by the National Natural Science Foundation of China,Nos.81471273(to JQ),and 81472088(to CLS)the Natural Science Research Projects in Colleges and Universities of Anhui Province,No.KJ2020ZD23(to JQ)the Natural Science Foundation of Anhui Province,No.2208085MH210(to JQ)。
文摘Spinal cord injury often leads to severe motor and sensory deficits,and prognosis using the currently available therapies remains poor.Therefore,we aimed to explore a novel therapeutic approach for improving the prognosis of spinal cord injury.In this study,we implanted oscillating field stimulation devices and transplanted neural stem cells into the thoracic region(T9–T10)of rats with a spinal cord contusion.Basso-Beattie-Bresnahan scoring revealed that oscillating field stimulation combined with neural stem cells transplantation promoted motor function recovery following spinal cord injury.In addition,we investigated the regulation of oscillating field stimulation on the miR-124/Tal1 axis in neural stem cells.Transfection of lentivirus was performed to investigate the role of Tal1 in neurogenesis of neural stem cells induced by oscillating field stimulation.Quantitative reverse transcription-polymerase chain reaction,immunofluorescence and western blotting showed that oscillating field stimulation promoted neurogenesis of neural stem cells in vitro and in vivo.Hematoxylin and eosin staining showed that oscillating field stimulation combined with neural stem cells transplantation alleviated cavities formation after spinal cord injury.Taking the results together,we concluded that oscillating field stimulation decreased miR-124 expression and increased Tal1 content,thereby promoting the neurogenesis of neural stem cells.The combination of oscillating field stimulation and neural stem cells transplantation improved neurogenesis,and thereby promoted structural and functional recovery after spinal cord injury.
基金supported by Helene Houle Career Development Award in Neurologic Surgery Research and Fund for Mayo Clinic Center for Regenerative Medicine Program Director,Neuroregenerative Medicine,Mayo Clinic College of Medicine and Science.SG was supported by the Chinese Scholarship Council.
文摘Chronic denervation is one of the key factors that affect nerve regeneration.Chronic axotomy deteriorates the distal nerve stump,causes protein changes,and renders the microenvironment less permissive for regeneration.Some of these factors/proteins have been individually studied.To better delineate the comprehensive protein expression profiles and identify proteins that contribute to or are associated with this detrimental effect,we carried out a proteomic analysis of the distal nerve using an established delayed rat sciatic nerve repair model.Four rats that received immediate repair after sciatic nerve transection served as control,whereas four rats in the experimental group(chronic denervation)had their sciatic nerve repaired after a 12-week delay.All the rats were sacrificed after 16 weeks to harvest the distal nerves for extracting proteins.Twenty-five micrograms of protein from each sample were fractionated in SDS-PAGE gels.NanoLC-MS/MS analysis was applied to the gels.Protein expression levels of nerves on the surgery side were compared to those on the contralateral side.Any protein with a P value of less than 0.05 and a fold change of 4 or higher was deemed differentially expressed.All the differentially expressed proteins in both groups were further stratified according to the biological processes.A PubMed search was also conducted to identify the differentially expressed proteins that have been reported to be either beneficial or detrimental to nerve regeneration.Ingenuity Pathway Analysis(IPA)software was used for pathway analysis.The results showed that 709 differentially expressed proteins were identified in the delayed repair group,with a bigger proportion of immune and inflammatory process-related proteins and a smaller proportion of proteins related to axon regeneration and lipid metabolism in comparison to the control group where 478 differentially expressed proteins were identified.The experimental group also had more beneficial proteins that were downregulated and more detrimental proteins that were upregulated.IPA revealed that protective pathways such as LXR/RXR,acute phase response,RAC,ERK/MAPK,CNTF,IL-6,and FGF signaling were inhibited in the delayed repair group,whereas three detrimental pathways,including the complement system,PTEN,and apoptosis signaling,were activated.An available database of the adult rodent sciatic nerve was used to assign protein changes to specific cell types.The poor regeneration seen in the delayed repair group could be associated with the down-regulation of beneficial proteins and up-regulation of detrimental proteins.The proteins and pathways identified in this study may offer clues for future studies to identify therapeutic targets.
文摘随着纠删码在分布式存储系统中的实际应用,纠删码为存储系统提供了更加优秀的存储效率,但当节点丢失时,相较于传统副本技术更多的网络传输带宽开销成为了造成系统性能瓶颈的关键因素。为了解决MDS编码高带宽开销对系统性能的影响,一类新型编码方案——分组码被应用在分布式存储系统中,相较于传统MDS编码能够有效地降低节点修复时的数据传输量,从而减少网络带宽需求。在Pyramid分组码的基础上进行层次扩展,提出一种HLRC(hierarchical local repair codes)纠删码。HLRC相较于LRC引入了层次编码模型,将原始数据块构建为编码矩阵,根据层次进行分别编码,生成包含数据块范围不同的局部校验块;每个层次包含的数据块数量不同,可以保证修复节点时的低修复成本,同时还拥有较高的存储效率。HLRC相较于Pyramid拥有额外的校验块冗余,能够降低校验块出错和多节点出错时的恢复开销。在基于Ceph的分布式存储系统中的实验结果表明,HLRC与Pyramid等分组码相比,单节点修复开销最高可降低48.56%,多节点修复开销最高可降低25%。
文摘连线干涉测量(Connected Element Interferometry,CEI)是一种全天时全天候的被动测角技术,已用于空间目标的跟踪监视.地球静止轨道(Geostationary Earth Orbit,GEO)卫星需要频繁机动以保持轨位或完成其他任务,其机动后的快速轨道恢复能力对于监视预警极为重要.针对基于CEI的GEO短弧定轨和预报,分析了定轨算法的形亏和数亏,在附加先验轨道约束的短弧定轨基础上,提出了轨道半长轴初值的自适应优化方法.利用亚太七号卫星的CEI仿真和实测数据进行了短弧定轨和预报,实验结果表明,采用优化后的半长轴初值,30min短弧定轨和10min预报的卫星位置分量精度均优于4km,能够满足非合作GEO目标机动后快速轨道恢复的需求.
文摘快速可行的灾后恢复是弹性电力系统的重要一环。提出一种弹性导向的考虑修复不确定性和V2G(vehicle to grid,V2G)的城市电力系统动态供电恢复方法,灾后短期内应用V2G站中的有限电动汽车资源为城市电网提供电能支撑,同时派遣抢修队对故障线路进行抢修作业,实现系统快速恢复。对V2G和电动汽车灾前、灾后行为进行建模,通过灾前自由调度统计各V2G站附近的电动汽车数量,按照响应意愿比例得到灾后阶段各V2G站反向输电功率;对包含行驶行为和修复行为的动态修复行为进行建模,采用滚动时域优化方法决策修复的先后顺序,得到抢修队最优动态修复方案。最后,设计多组算例以验证所提方法的可行性,结果表明,该方法可有效支撑灾后城市电力系统快速恢复,提高系统弹性。
基金supported by the Lorenz B?hler Fonds,#2/19 (obtained by the Neuroregeneration Group,Ludwig Boltzmann Institute for Traumatology)the City of Vienna project ImmunTissue,MA23#30-11 (obtained by the Department Life Science Engineering,University of Applied Sciences Technikum Wien)。
文摘Peripheral nerve injuries induce a severe motor and sensory deficit. Since the availability of autologous nerve transplants for nerve repair is very limited, alternative treatment strategies are sought, including the use of tubular nerve guidance conduits(tNGCs). However, the use of tNGCs results in poor functional recovery and central necrosis of the regenerating tissue, which limits their application to short nerve lesion defects(typically shorter than 3 cm). Given the importance of vascularization in nerve regeneration, we hypothesized that enabling the growth of blood vessels from the surrounding tissue into the regenerating nerve within the tNGC would help eliminate necrotic processes and lead to improved regeneration. In this study, we reported the application of macroscopic holes into the tubular walls of silk-based tNGCs and compared the various features of these improved silk^(+) tNGCs with the tubes without holes(silk^(–) tNGCs) and autologous nerve transplants in an 8-mm sciatic nerve defect in rats. Using a combination of micro-computed tomography and histological analyses, we were able to prove that the use of silk^(+) tNGCs induced the growth of blood vessels from the adjacent tissue to the intraluminal neovascular formation. A significantly higher number of blood vessels in the silk^(+) group was found compared with autologous nerve transplants and silk^(–), accompanied by improved axon regeneration at the distal coaptation point compared with the silk^(–) tNGCs at 7 weeks postoperatively. In the 15-mm(critical size) sciatic nerve defect model, we again observed a distinct ingrowth of blood vessels through the tubular walls of silk^(+) tNGCs, but without improved functional recovery at 12 weeks postoperatively. Our data proves that macroporous tNGCs increase the vascular supply of regenerating nerves and facilitate improved axonal regeneration in a short-defect model but not in a critical-size defect model. This study suggests that further optimization of the macroscopic holes silk^(+) tNGC approach containing macroscopic holes might result in improved grafting technology suitable for future clinical use.
文摘为了减弱极端事件对综合能源系统的影响,尽早恢复各类负荷,提高系统弹性。文中提出了一种考虑交通网与配电网级别的区域电-气互联系统(Interconnection Electric and Gas System, IEGS)协同运行的灾后故障抢修策略。首先,通过元胞传输模型建立了考虑系统实时恢复指标修正的交通流量分配模型,对交通网的交通流量进行预测;其次,在故障预分配的前提下建立了区域IEGS的故障恢复模型,协调优化维修队、分布式电源、配电网网架等资源,减少停电损失;然后以维修队的最小通行时间矩阵以及由系统实时恢复指标得到的交通网道路修正参数作为传递变量,在维修队开始移动的时间点,重新计算后续的修复计划以及交通网的运行情况,进行多时间断面优化;最后,通过算例仿真进行对比,验证了所提策略的有效性,并分析移动储能装置对故障恢复的影响。