The Myc gene is the essential oncogene in triple-negative breast cancer(TNBC).This study investigates the synergistic effects of combining Myc decoy oligodeoxynucleotides-encapsulated niosomes-selenium hybrid nanocarr...The Myc gene is the essential oncogene in triple-negative breast cancer(TNBC).This study investigates the synergistic effects of combining Myc decoy oligodeoxynucleotides-encapsulated niosomes-selenium hybrid nanocarriers with X-irradiation exposure on the MDA-MB-468 cell line.Decoy and scramble ODNs for Myc transcription factor were designed and synthesized based on promoter sequences of the Bcl2 gene.The nanocarriers were synthesized by loading Myc ODNs and selenium into chitosan(Chi-Se-DEC),which was then encapsulated in niosome-nanocarriers(NISM@Chi-Se-DEC).FT-IR,DLS,FESEM,and hemolysis tests were applied to confirm its characterization and physicochemical properties.Moreover,cellular uptake,cellular toxicity,apoptosis,cell cycle,and scratch repair assays were performed to evaluate its anticancer effects on cancer cells.All anticancer assessments were repeated under X-ray irradiation conditions(fractionated 2Gy).Physicochemical characteristics of niosomes containing SeNPs and ODNs showed that it is synthesized appropriately.It revealed that the anticancer effect of NISM@Chi-Se-DEC can be significantly improved in combination with X-ray irradiation treatment.It can be concluded that NISM@Chi-Se-DEC nanocarriers have the potential as a therapeutic agent for cancer treatment,particularly in combination with radiation therapy and in-vivo experiments are necessary to confirm the efficacy of this nano-drug.展开更多
Diffusions of multiple components have numerous applications such as underground water flow, pollutant movement, stratospheric warming, and food processing. Particularly, liquid hydrogen is used in the cooling process...Diffusions of multiple components have numerous applications such as underground water flow, pollutant movement, stratospheric warming, and food processing. Particularly, liquid hydrogen is used in the cooling process of the aeroplane. Further, liquid nitrogen can find applications in cooling equipment or electronic devices, i.e., high temperature superconducting(HTS) cables. So, herein, we have analysed the entropy generation(EG), nonlinear thermal radiation and unsteady(time-dependent) nature of the flow on quadratic combined convective flow over a permeable slender cylinder with diffusions of liquid hydrogen and nitrogen. The governing equations for flow and heat transfer characteristics are expressed in terms of nonlinear coupled partial differential equations. The solutions of these equations are attempted numerically by employing the quasilinearization technique with the implicit finite difference approximation. It is found that EG is minimum for double diffusion(liquid hydrogen and heat diffusion)than triple diffusion(diffusion of liquid hydrogen, nitrogen and heat). The enhancing values of the radiation parameter R_(d) and temperature ratio θ_(w) augment the fluid temperature for steady and unsteady cases as well as the local Nusselt number. Because, the fluid absorbs the heat energy released due to radiation, and in turn releases the heat energy from the cylinder to the surrounding surface.展开更多
Effects of the simulated acid rain（AR） and ultraviolet-B（UV-B, 280-320 nm） radiation with a single or two ways simultaneously （AR ＋ UV-B） on the antioxidant enzyme and photosynthesis of the rape seedlings were ...Effects of the simulated acid rain（AR） and ultraviolet-B（UV-B, 280-320 nm） radiation with a single or two ways simultaneously （AR ＋ UV-B） on the antioxidant enzyme and photosynthesis of the rape seedlings were investigated by the hydroponic culture. The results of static experiment indicated that the tolerance of rape seedling to single stress（AR or UV-B） is stronger than that to dual stresses（AR ＋ UV-B）. Furthermore, the dual stresses had additive effect on catalase activity, and a synergistic effect on MDA content, net photosynthesis rate, water use efficiency as well as intercellular CO2 concentration. Meanwhile, it has an independent effect on chlorophyll content, stomatal conductance, and transpiration rate as well as membrane permeability. During 64 h restoration course, the dynamic change in the curves of physiological and biochemical indices were not identical, and none of them show a simple linear variation. According to the static and dynamic experiments, it was found that a responsive sequence of catalase activity, membrane permeability, MDA content and photosynthetic characteristics to the above-mentioned stresses was as follows： AR ＋ UV-B 〉 UV-B 〉 AR.展开更多
A new remote sensing method is described to determine the vertical distribution and total content of atmospheric ozone. The method combines surface infrared, satellite infrared and ultraviolet channels. The width of t...A new remote sensing method is described to determine the vertical distribution and total content of atmospheric ozone. The method combines surface infrared, satellite infrared and ultraviolet channels. The width of the infrared channels is 0.01 cm-1, less than Lorentz half-width at the earth's surface, rather than the present width, because these channels can obtain information about variations in the ozone profile below the profile main-peak. The numerical experiments show that the method has a satisfactory precision in determining total ozone content, just about I percent error, and vertical distribution from the earth to 65 km space. In addition, some semi-analysis functions lor calculating backscattered ultraviolet and a relaxation equation are described in this paper.展开更多
A nondestructive X-ray analysis technique combining transmission tomography, fluorescence tomography and Compton tomography based on synchrotron radiation is described. This novel technique will be an optional experim...A nondestructive X-ray analysis technique combining transmission tomography, fluorescence tomography and Compton tomography based on synchrotron radiation is described. This novel technique will be an optional experimental technique at SSRF's hard X-ray micro-focusing beamline under construction at present. An experimental result of combined X-ray tomography is obtained in NE-5A station of PF. The reconstructed images of test objects are given.展开更多
Selectively killing cancer without harming normal tissue is a fundamental challenge in cancer therapy. Elevatedoxidative stress and aberrant redox homeostasis are frequently observed in cancer cells compared with thei...Selectively killing cancer without harming normal tissue is a fundamental challenge in cancer therapy. Elevatedoxidative stress and aberrant redox homeostasis are frequently observed in cancer cells compared with their normalcell counterparts[1]. A small shift toward an oxidizing condition in cells may lead to elevated proliferation andinduction of adaptive response. However, a high oxidizing condition often results in cell injury and cell death.Persistent high level of reactive oxygen species (ROS) in cancer cells usually elicits increased cell proliferation andadaptive responses that may contribute to tumorigenesis, metastasis, and treatment resistance. However, normalcells may still maintain redox homeostasis through adaptive responses. Therefore, regulating intracellular redoxstate may represent an ideal strategy to selectively sensitize cancer cells to oxidative stress-inducing therapy, suchas radiotherapy.展开更多
The effects of pre-irradiation blood transfusion(BT)on survival rateof radiation-burn combinedly injured rats receiving bone marrow transplantation(BMT) were studied. It was found that after 9-11 Gy of radiation was g...The effects of pre-irradiation blood transfusion(BT)on survival rateof radiation-burn combinedly injured rats receiving bone marrow transplantation(BMT) were studied. It was found that after 9-11 Gy of radiation was given, the 90-daysurvival rate of the rats receiving BT(72%) and BMT was significantly higher than thatof those receiving BMT only(42%)(P<0.01).In those rats surviving over 100 days,cells of donor type could be found. In the first 30 days of surviving, the number of Tcells was significantly higher in the BMT alone group than in the group of BMT plusBT, but no difference In restoration of B cell was revealed. The findings suggest that BTcould promote the recipient's tolerance to BMT. The effects of BT on BMT are similarto those on skin grafting.展开更多
Nitric oxide formation and cyclic GMP level in the myocardium were studied in the early stage after radiation, bum andcombined radiation-bum injuries in rats. Nitric oxide synthase (NOS) activity was measured in the c...Nitric oxide formation and cyclic GMP level in the myocardium were studied in the early stage after radiation, bum andcombined radiation-bum injuries in rats. Nitric oxide synthase (NOS) activity was measured in the cytosol of the left ventricularwall. In the controls, the cytosol was found to contain mainly Ca2+ -dependent NOS (cNOS) and a small amount of Ca2+ -inden-pendent NOS (iNOS). After burn and combined radiation-burn injuries, a marked increase of iNOS activity with a peak in the 8thhour postinjury was found but the myocardial cNOS activity declined obviously. Parallel to iNOS activity increase, there was a significant increase of myocardial production of NO and cGMP. The combined effcts of radiation and burn injuries on the rats weremore severe than those of burn injury alone. All the changes could be prevented by the administration of dexamethasone. No obvious changes were observed in the rats after radiation injury alone. Since the increase of cGMP level in the heart is associated withreduced contractility, it is possible that the increased production of NO stimulated by iNOS accounts at least partially, for the depression of myocardial contractility after bum and combined radiation burn injury.展开更多
A study of a combination of Weather Research and Forecasting (WRF) model and Long Short Term Memory (LSTM) network for location in Dili Timor Leste is introduced in this paper. One calendar year’s results of solar ra...A study of a combination of Weather Research and Forecasting (WRF) model and Long Short Term Memory (LSTM) network for location in Dili Timor Leste is introduced in this paper. One calendar year’s results of solar radiation from January to December 2014 are used as input data to estimate future forecasting of solar radiation using the LSTM network for three months period. The WRF model version 3.9.1 is used to simulate one year’s solar radiation in horizontal resolution low scale for nesting domain 1</span><span style="font-family:""> </span><span style="font-family:Verdana;">×</span><span style="font-family:""> </span><span style="font-family:Verdana;">1 km. It is done by applying 6-hourly interval 1</span><span style="font-family:Verdana;">º</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">×</span><span style="font-family:Verdana;"> 1</span></span><span style="font-family:Verdana;">º</span><span style="font-family:""><span style="font-family:Verdana;"> NCEP FNL analysis data used as Global Forecast System (GFS). LSTM network is applied for forecasting in numerous learning problems for solar radiation forecasting. LSTM network uses two-layer LSTM architecture of 512 hidden neurons coupled with a dense output layer with linear as the model activation to predict with time steps are configured to 50 and the number of features is 1. The maximum epoch is set to 325 with batch size 300 and the validation split is 0.09. The results demonstrate that the combination of these two methods can successfully predict solar radiation where four error metrics of mean bias error (MBE), root mean square error (RMSE), normalized MBE (nMBE), and normalized RMSE (nRMSE) perform small error distribution and percentage in three months prediction where the error percentage is obtained below the 20% for nMBE and nRMSE. Meanwhile, the error distribution of RMSE is obtained below 200 W/m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> and maximum bias error is 0.07. Finally, the values of MBE, RMSE, nMBE, and nRMSE conclude that the good performance of the combination of two methods in this study can be applied to simulate any other weather variable for local necessary.展开更多
With the development of radiotherapeutic oncology, computer technology and medical imaging technology, radiation therapy has made great progress. Research on the impact and the specific mechanism of radiation on tumor...With the development of radiotherapeutic oncology, computer technology and medical imaging technology, radiation therapy has made great progress. Research on the impact and the specific mechanism of radiation on tumors has become a central topic in cancer therapy. According to the traditional view, radiation can directly affect the structure of the DNA double helix, which in turn activates DNA damage sensors to induce apoptosis, necrosis, and aging or affects normal mitosis events and ultimately rewires various biological characteristics of neoplasm cells. In addition, irradiation damages subcellular structures, such as the cytoplasmic membrane, endoplasmic reticulum, ribosome, mitochondria, and lysosome of cancer cells to regulate various biological activities of tumor cells. Recent studies have shown that radiation can also change the tumor cell phenotype, immunogenicity and microenvironment, thereby globally altering the biological behavior of cancer cells. In this review, we focus on the effects of therapeutic radiation on the biological features of tumor cells to provide a theoretical basis for combinational therapy and inaugurate a new era in oncology.展开更多
Stereotactic body radiation therapy(SBRT) is an emerging treatment for hepatocellular carcinoma. This technique results in excellent local control rates with favorable toxicity profile despite being predominantly used...Stereotactic body radiation therapy(SBRT) is an emerging treatment for hepatocellular carcinoma. This technique results in excellent local control rates with favorable toxicity profile despite being predominantly used in heavily pretreated patients or those unsuitable for other local therapies. SBRT may be used as a sole treatment or in combination with other local therapies as well as a bridging strategy for patient awaiting liver transplants. This brief review describes current practice of SBRT with respect to radiation technique, patient selection and treatment concepts. It summarizes available evidence from retroand prospective studies evaluating SBRT alone, SBRT in combination with other treatments and SBRT compared to other local treatment approaches.展开更多
基金supported by Zanjan University of Medical Sciences,Zanjan,Iran(Grant Number:A-12-1244-16&Ethical Code:IR.ZUMS.REC.1399.316).
文摘The Myc gene is the essential oncogene in triple-negative breast cancer(TNBC).This study investigates the synergistic effects of combining Myc decoy oligodeoxynucleotides-encapsulated niosomes-selenium hybrid nanocarriers with X-irradiation exposure on the MDA-MB-468 cell line.Decoy and scramble ODNs for Myc transcription factor were designed and synthesized based on promoter sequences of the Bcl2 gene.The nanocarriers were synthesized by loading Myc ODNs and selenium into chitosan(Chi-Se-DEC),which was then encapsulated in niosome-nanocarriers(NISM@Chi-Se-DEC).FT-IR,DLS,FESEM,and hemolysis tests were applied to confirm its characterization and physicochemical properties.Moreover,cellular uptake,cellular toxicity,apoptosis,cell cycle,and scratch repair assays were performed to evaluate its anticancer effects on cancer cells.All anticancer assessments were repeated under X-ray irradiation conditions(fractionated 2Gy).Physicochemical characteristics of niosomes containing SeNPs and ODNs showed that it is synthesized appropriately.It revealed that the anticancer effect of NISM@Chi-Se-DEC can be significantly improved in combination with X-ray irradiation treatment.It can be concluded that NISM@Chi-Se-DEC nanocarriers have the potential as a therapeutic agent for cancer treatment,particularly in combination with radiation therapy and in-vivo experiments are necessary to confirm the efficacy of this nano-drug.
文摘Diffusions of multiple components have numerous applications such as underground water flow, pollutant movement, stratospheric warming, and food processing. Particularly, liquid hydrogen is used in the cooling process of the aeroplane. Further, liquid nitrogen can find applications in cooling equipment or electronic devices, i.e., high temperature superconducting(HTS) cables. So, herein, we have analysed the entropy generation(EG), nonlinear thermal radiation and unsteady(time-dependent) nature of the flow on quadratic combined convective flow over a permeable slender cylinder with diffusions of liquid hydrogen and nitrogen. The governing equations for flow and heat transfer characteristics are expressed in terms of nonlinear coupled partial differential equations. The solutions of these equations are attempted numerically by employing the quasilinearization technique with the implicit finite difference approximation. It is found that EG is minimum for double diffusion(liquid hydrogen and heat diffusion)than triple diffusion(diffusion of liquid hydrogen, nitrogen and heat). The enhancing values of the radiation parameter R_(d) and temperature ratio θ_(w) augment the fluid temperature for steady and unsteady cases as well as the local Nusselt number. Because, the fluid absorbs the heat energy released due to radiation, and in turn releases the heat energy from the cylinder to the surrounding surface.
文摘Effects of the simulated acid rain（AR） and ultraviolet-B（UV-B, 280-320 nm） radiation with a single or two ways simultaneously （AR ＋ UV-B） on the antioxidant enzyme and photosynthesis of the rape seedlings were investigated by the hydroponic culture. The results of static experiment indicated that the tolerance of rape seedling to single stress（AR or UV-B） is stronger than that to dual stresses（AR ＋ UV-B）. Furthermore, the dual stresses had additive effect on catalase activity, and a synergistic effect on MDA content, net photosynthesis rate, water use efficiency as well as intercellular CO2 concentration. Meanwhile, it has an independent effect on chlorophyll content, stomatal conductance, and transpiration rate as well as membrane permeability. During 64 h restoration course, the dynamic change in the curves of physiological and biochemical indices were not identical, and none of them show a simple linear variation. According to the static and dynamic experiments, it was found that a responsive sequence of catalase activity, membrane permeability, MDA content and photosynthetic characteristics to the above-mentioned stresses was as follows： AR ＋ UV-B 〉 UV-B 〉 AR.
文摘A new remote sensing method is described to determine the vertical distribution and total content of atmospheric ozone. The method combines surface infrared, satellite infrared and ultraviolet channels. The width of the infrared channels is 0.01 cm-1, less than Lorentz half-width at the earth's surface, rather than the present width, because these channels can obtain information about variations in the ozone profile below the profile main-peak. The numerical experiments show that the method has a satisfactory precision in determining total ozone content, just about I percent error, and vertical distribution from the earth to 65 km space. In addition, some semi-analysis functions lor calculating backscattered ultraviolet and a relaxation equation are described in this paper.
文摘A nondestructive X-ray analysis technique combining transmission tomography, fluorescence tomography and Compton tomography based on synchrotron radiation is described. This novel technique will be an optional experimental technique at SSRF's hard X-ray micro-focusing beamline under construction at present. An experimental result of combined X-ray tomography is obtained in NE-5A station of PF. The reconstructed images of test objects are given.
文摘Selectively killing cancer without harming normal tissue is a fundamental challenge in cancer therapy. Elevatedoxidative stress and aberrant redox homeostasis are frequently observed in cancer cells compared with their normalcell counterparts[1]. A small shift toward an oxidizing condition in cells may lead to elevated proliferation andinduction of adaptive response. However, a high oxidizing condition often results in cell injury and cell death.Persistent high level of reactive oxygen species (ROS) in cancer cells usually elicits increased cell proliferation andadaptive responses that may contribute to tumorigenesis, metastasis, and treatment resistance. However, normalcells may still maintain redox homeostasis through adaptive responses. Therefore, regulating intracellular redoxstate may represent an ideal strategy to selectively sensitize cancer cells to oxidative stress-inducing therapy, suchas radiotherapy.
文摘The effects of pre-irradiation blood transfusion(BT)on survival rateof radiation-burn combinedly injured rats receiving bone marrow transplantation(BMT) were studied. It was found that after 9-11 Gy of radiation was given, the 90-daysurvival rate of the rats receiving BT(72%) and BMT was significantly higher than thatof those receiving BMT only(42%)(P<0.01).In those rats surviving over 100 days,cells of donor type could be found. In the first 30 days of surviving, the number of Tcells was significantly higher in the BMT alone group than in the group of BMT plusBT, but no difference In restoration of B cell was revealed. The findings suggest that BTcould promote the recipient's tolerance to BMT. The effects of BT on BMT are similarto those on skin grafting.
文摘Nitric oxide formation and cyclic GMP level in the myocardium were studied in the early stage after radiation, bum andcombined radiation-bum injuries in rats. Nitric oxide synthase (NOS) activity was measured in the cytosol of the left ventricularwall. In the controls, the cytosol was found to contain mainly Ca2+ -dependent NOS (cNOS) and a small amount of Ca2+ -inden-pendent NOS (iNOS). After burn and combined radiation-burn injuries, a marked increase of iNOS activity with a peak in the 8thhour postinjury was found but the myocardial cNOS activity declined obviously. Parallel to iNOS activity increase, there was a significant increase of myocardial production of NO and cGMP. The combined effcts of radiation and burn injuries on the rats weremore severe than those of burn injury alone. All the changes could be prevented by the administration of dexamethasone. No obvious changes were observed in the rats after radiation injury alone. Since the increase of cGMP level in the heart is associated withreduced contractility, it is possible that the increased production of NO stimulated by iNOS accounts at least partially, for the depression of myocardial contractility after bum and combined radiation burn injury.
文摘A study of a combination of Weather Research and Forecasting (WRF) model and Long Short Term Memory (LSTM) network for location in Dili Timor Leste is introduced in this paper. One calendar year’s results of solar radiation from January to December 2014 are used as input data to estimate future forecasting of solar radiation using the LSTM network for three months period. The WRF model version 3.9.1 is used to simulate one year’s solar radiation in horizontal resolution low scale for nesting domain 1</span><span style="font-family:""> </span><span style="font-family:Verdana;">×</span><span style="font-family:""> </span><span style="font-family:Verdana;">1 km. It is done by applying 6-hourly interval 1</span><span style="font-family:Verdana;">º</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">×</span><span style="font-family:Verdana;"> 1</span></span><span style="font-family:Verdana;">º</span><span style="font-family:""><span style="font-family:Verdana;"> NCEP FNL analysis data used as Global Forecast System (GFS). LSTM network is applied for forecasting in numerous learning problems for solar radiation forecasting. LSTM network uses two-layer LSTM architecture of 512 hidden neurons coupled with a dense output layer with linear as the model activation to predict with time steps are configured to 50 and the number of features is 1. The maximum epoch is set to 325 with batch size 300 and the validation split is 0.09. The results demonstrate that the combination of these two methods can successfully predict solar radiation where four error metrics of mean bias error (MBE), root mean square error (RMSE), normalized MBE (nMBE), and normalized RMSE (nRMSE) perform small error distribution and percentage in three months prediction where the error percentage is obtained below the 20% for nMBE and nRMSE. Meanwhile, the error distribution of RMSE is obtained below 200 W/m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> and maximum bias error is 0.07. Finally, the values of MBE, RMSE, nMBE, and nRMSE conclude that the good performance of the combination of two methods in this study can be applied to simulate any other weather variable for local necessary.
基金financially supported by grants from the CAMS Innovation Fund for Medical Sciences(CIFMS)(No.2016-I2M-1-001)the National Basic Research Program of China(973 Program)(No.2015CB553904)+1 种基金the National Natural Science Foundation of China(No.81372158,81372159,81572842,81672459)the Independent Issue of State Key Laboratory of Molecular Oncology(No.SKL-2017-16)
文摘With the development of radiotherapeutic oncology, computer technology and medical imaging technology, radiation therapy has made great progress. Research on the impact and the specific mechanism of radiation on tumors has become a central topic in cancer therapy. According to the traditional view, radiation can directly affect the structure of the DNA double helix, which in turn activates DNA damage sensors to induce apoptosis, necrosis, and aging or affects normal mitosis events and ultimately rewires various biological characteristics of neoplasm cells. In addition, irradiation damages subcellular structures, such as the cytoplasmic membrane, endoplasmic reticulum, ribosome, mitochondria, and lysosome of cancer cells to regulate various biological activities of tumor cells. Recent studies have shown that radiation can also change the tumor cell phenotype, immunogenicity and microenvironment, thereby globally altering the biological behavior of cancer cells. In this review, we focus on the effects of therapeutic radiation on the biological features of tumor cells to provide a theoretical basis for combinational therapy and inaugurate a new era in oncology.
文摘Stereotactic body radiation therapy(SBRT) is an emerging treatment for hepatocellular carcinoma. This technique results in excellent local control rates with favorable toxicity profile despite being predominantly used in heavily pretreated patients or those unsuitable for other local therapies. SBRT may be used as a sole treatment or in combination with other local therapies as well as a bridging strategy for patient awaiting liver transplants. This brief review describes current practice of SBRT with respect to radiation technique, patient selection and treatment concepts. It summarizes available evidence from retroand prospective studies evaluating SBRT alone, SBRT in combination with other treatments and SBRT compared to other local treatment approaches.
