Coralyne is an important alkaloid due to its anti-cancer and other medicinal properties.It targets DNA in cells and acts as human topoisomerase-I poison,telomerase inhibitor and nucleic acid intercalator.It has high t...Coralyne is an important alkaloid due to its anti-cancer and other medicinal properties.It targets DNA in cells and acts as human topoisomerase-I poison,telomerase inhibitor and nucleic acid intercalator.It has high tendency to undergo self-association,which is a matter of concern for therapeutic applications.The understanding of its interaction with DNA requires precise knowledge of chemical shifts in Nuclear Magnetic Resonance(NMR)spectra besides self-association.The present study is the first report of a complete assignment of all 1H/13C resonances in NMR spectra of coralyne in DMSO-d6 using one dimensional 1H/13C and two dimensional NMR experiments.The chemical shift of all proton and several 13C resonances have also been obtained in D2O and ethanol-d6.The same has been calculated using Density Functional Theory(DFT).NMR spectra of coralyne show upfield shift of 0.6e1.2 ppm in aromatic ring protons suggesting stacking interactions.Apart from 11 intra molecular NOE cross peaks in 2D 1H e1H ROESY spectra,3 short distance NOE correlations,H6e10OCH3,H5e10OCH3 and H12e16CH3,give direct independent evidence of the formation of a stacked dimer.The absorbance,fluorescence,circular dichroism and fluorescence lifetime experiments conducted in the present investigations corroborate results obtained by NMR.展开更多
基金Ministry of Human Resources and Development(MHRD),Government of India for the support for funding
文摘Coralyne is an important alkaloid due to its anti-cancer and other medicinal properties.It targets DNA in cells and acts as human topoisomerase-I poison,telomerase inhibitor and nucleic acid intercalator.It has high tendency to undergo self-association,which is a matter of concern for therapeutic applications.The understanding of its interaction with DNA requires precise knowledge of chemical shifts in Nuclear Magnetic Resonance(NMR)spectra besides self-association.The present study is the first report of a complete assignment of all 1H/13C resonances in NMR spectra of coralyne in DMSO-d6 using one dimensional 1H/13C and two dimensional NMR experiments.The chemical shift of all proton and several 13C resonances have also been obtained in D2O and ethanol-d6.The same has been calculated using Density Functional Theory(DFT).NMR spectra of coralyne show upfield shift of 0.6e1.2 ppm in aromatic ring protons suggesting stacking interactions.Apart from 11 intra molecular NOE cross peaks in 2D 1H e1H ROESY spectra,3 short distance NOE correlations,H6e10OCH3,H5e10OCH3 and H12e16CH3,give direct independent evidence of the formation of a stacked dimer.The absorbance,fluorescence,circular dichroism and fluorescence lifetime experiments conducted in the present investigations corroborate results obtained by NMR.
