Based on the genetic clustering from 42 microsatellite （SSR） markers with a combination of their geographic origin and germplasm characteristics, 124 maize landraces from Wuling Mountain region in China were used fo...Based on the genetic clustering from 42 microsatellite （SSR） markers with a combination of their geographic origin and germplasm characteristics, 124 maize landraces from Wuling Mountain region in China were used for constructing a core collection. Four evaluating parameters for maize landrace core collection, including mean difference percentage （MD）, variance difference percentage （VD）, coincidence rate of range （CR）, and variable rate of coefficient of variation （VR）, were assessed With 20 quantitative traits. It was shown that genetic relationships among landraces in Wuling Mountain region had the tendency to associate with their geographic origins. The 124 landraces were clustered into 18 subgroups when the coefficient of genetic similarity （GS） is 0.28. Eighteen landraces, each of which was from one subgroup, were applied to construct the core collection with a sampling percentage of 15%. Comparison of the initial and core collection indicated that there existed no significant differences in most quantitative traits. An average of 6.3 and 6.5 alleles were detected in the initial and core collection, respectively. Mean polymorphism information content in the core collection （0.75） was higher than that in the initial one （0.72）. MD was lesser than 20% and CR was more than 80%. The results showed that the sampling strategy would be feasible for constructing the core collection that well represents the genetic diversity of the initial one.展开更多
田间试验结合微卫星（ SSR ）标记技术，从表型和 DNA 分子水平分析评价了武陵山区玉米（Zea mays L.）地方品种核心种质的遗传多样性。结果表明，核心种质在14个农艺、经济性状上存在极显著差异；基于SSR标记的UPGMA聚类能有效区分玉...田间试验结合微卫星（ SSR ）标记技术，从表型和 DNA 分子水平分析评价了武陵山区玉米（Zea mays L.）地方品种核心种质的遗传多样性。结果表明，核心种质在14个农艺、经济性状上存在极显著差异；基于SSR标记的UPGMA聚类能有效区分玉米地方品种核心种质，50对SSR引物在核心种质中共检测到275个多态性位点，各引物检测到的等位位点数3~15个，平均为6.0个；Shannon多态信息量为0.29~0.83，平均为0.75。核心种质代表性评价结果表明核心种质较好地保持了原种质库的遗传变异。展开更多
基金the National High Tech-nology Research and Development Program of China(863 Program, 2004BA525B04)Program for Changjiang Scholars and Innovative Research Team in University of China (IRTO453)
文摘Based on the genetic clustering from 42 microsatellite （SSR） markers with a combination of their geographic origin and germplasm characteristics, 124 maize landraces from Wuling Mountain region in China were used for constructing a core collection. Four evaluating parameters for maize landrace core collection, including mean difference percentage （MD）, variance difference percentage （VD）, coincidence rate of range （CR）, and variable rate of coefficient of variation （VR）, were assessed With 20 quantitative traits. It was shown that genetic relationships among landraces in Wuling Mountain region had the tendency to associate with their geographic origins. The 124 landraces were clustered into 18 subgroups when the coefficient of genetic similarity （GS） is 0.28. Eighteen landraces, each of which was from one subgroup, were applied to construct the core collection with a sampling percentage of 15%. Comparison of the initial and core collection indicated that there existed no significant differences in most quantitative traits. An average of 6.3 and 6.5 alleles were detected in the initial and core collection, respectively. Mean polymorphism information content in the core collection （0.75） was higher than that in the initial one （0.72）. MD was lesser than 20% and CR was more than 80%. The results showed that the sampling strategy would be feasible for constructing the core collection that well represents the genetic diversity of the initial one.
文摘田间试验结合微卫星（ SSR ）标记技术，从表型和 DNA 分子水平分析评价了武陵山区玉米（Zea mays L.）地方品种核心种质的遗传多样性。结果表明，核心种质在14个农艺、经济性状上存在极显著差异；基于SSR标记的UPGMA聚类能有效区分玉米地方品种核心种质，50对SSR引物在核心种质中共检测到275个多态性位点，各引物检测到的等位位点数3~15个，平均为6.0个；Shannon多态信息量为0.29~0.83，平均为0.75。核心种质代表性评价结果表明核心种质较好地保持了原种质库的遗传变异。
