Geographical differences of leaf traits of the endangered plant Litsea coreana Levl.var.sinensis and its relationship with climate

Seventeen morphological and anatomical characteristics of the leaves were selected from five natural populations to explore the variation in leaf traits of Lits ea core ana var.sinensis and the effects of geographical environment on these variations.Nested analysis of variance,multiple comparisons,principal component analysis(PCA),and correlation analysis were conducted to explore the variations within and between populations and their correlation with geographical and climatic factors.Significant differences in the 17 leaf traits were observed within and among populations.On average,the relative contribution of within population variation to total variation was 24.8%,which was lower than among population variation(54.6%).The average differentiation coefficient of the traits was 65.8%,and the average coefficient of variation 11.8%,ranging from6.7%for main vein thickness to 21.4%for petiole length.The PC A results showed that morphological characteristics were divided into two categories,and the level of variation was greater than that of leaf anatomy.Most of the leaf traits were significantly correlated with geography and climate and showed a gradual variation with longitude,latitude,and altitude.In areas with high temperatures,less rainfall,and strong seasonal rainfall,the leaves are larger,longer and thicker.This study shows that variations in leaf traits of L.coreana var.sinensis mainly come from variations among populations.The level of trait differentiation among populations is high and the level of variation within populations low.These findings help further understand leaf morphological characteristics of this species and can provide a valuable reference for the protection and sustainable utilization of this natural resource.

Population dynamics and protection strategies of Litsea coreana Levl.var.sinensis with Guizhou Province in China as an example

Studying the dynamics of plant populations is crucial to the conservation and management of endangered plants.The diameter at breast height(DBH),tree height,crown width,and environmental factors of a total of 15 plots in five populations in Guizhou Province,China were measured in this study.The method of substituting diameter at breast height for age was used to divide the population into 7 age classes.The age structure,static life table,and relationship between the population characteristics and environmental factors of the Litsea coreana Levl.var.sinensis population were studied.Moreover,the time series model was used to predict the population dynamics.Results showed that the age structure of population in Zhengan County(Population ZA)had an inverted Jshape with abundant seedlings and insufficient middle-aged and elderly individuals.The age structures of populations in Daozhen County(Population DZ),Kaiyang County(KY),Meitan County(Population MT),and Xishui County(Population XS)were spindle-shaped with few young individuals and many middle-aged and elderly individuals.The mortality rate of each population fluctuated with the increase in age class,and the populations were extremely unstable.The survival curve was close to the Deevey III type,and the degree of deviation was related to the lack of young individuals.After the age stages II,IV,and VI,the number of aged plants in the different populations initially increased then decreased,and population stability was difficult to maintain.Redundancy analysis showed that the DBH,tree height and crown width of the population decreased with the increase of annual mean temperature,and the population density was restricted by soil nutrient conditions to a certain extent.The results of this work provide scientific basis for the conservation and sustainable utilization of natural populations of L.coreana var.sinensis in Guizhou Province,China.

贵州老鹰茶Litsea coreana的黄酮类化合物

从贵州老鹰茶Litseacoreana叶中分得 3个黄酮类化合物单体 ,经化学分析和波谱学方法确定了它们的化学结构 ,分别为山柰酚 。

老鹰茶(Litsea coreana Lvl.var.lanuginosa)多酚类化合物的提取纯化及EGCG含量测定

以老鹰茶(Litseacoreana Lvl.var.lanuginosa)粉末为原料,运用回流提取和聚酰胺树脂柱层析方法从中提取和纯化多酚类化合物.先用80%乙醇对老鹰茶粉末进行3次回流粗提,即提取温度100℃,提取时间30、20和10min,料液比1:20、1:10和1:10,多酚粗提率为31.46%;然后调节粗提液pH至2.0,12000r?min-1离心10min,得上清液,上清液中多酚得率为93.59%;用去离子水将上清液稀释到2mg?mL-1,调pH值至2.0,作为上样液,再利用聚酰胺树脂对上样液中的多酚类化合物进行纯化.结果表明:在柱高与直径比22,上样液体积1.5BV,吸附平衡1h,洗脱液依次为少量去离子水和80%乙醇,洗脱液流速6mL?min-1条件下,多酚洗脱率达到97.20%;洗脱液经浓缩后,冷冻真空干燥得到纯度为92.80%的多酚类化合物,多酚提取率为18.06%.高效液相色谱(HPLC)测定多酚类化合物中表没食子儿茶素没食子酸酯(EGCG)含量为49.82%.

Total flavonoid of Litsea coreana leve exerts anti-oxidative effects and alleviates focal cerebral ischemia/reperfusion injury

In this study, we hypothesized that total flavonoid of Litsea coreana leve （TFLC） protects against focal cerebral ischemia/reperfusion injury. TFLC （25, 50, 100 mg/kg） was administered orally to a rat model of focal ischemia/reperfusion injury, while the free radical scavenging agent, edaravone, was used as a positive control drug. Results of neurological deficit scoring, 2,3,5-triphenyl tetrazolium chloride staining, hematoxylin-eosin staining and biochemical tests showed that TFLC at different doses significantly alleviated cerebral ischemia-induced neurological deficits and histopathological changes, and reduced infarct volume. Moreover, it suppressed the increase in the levels of nitrates plus nitrites, malondialdehyde and lactate dehydrogenase, and it diminished the reduction in glu- tathione, superoxide dismutase and catalase activities induced by cerebral ischemia/reperfusion injury. Compared with edaravone, the protective effects of TFLC at low and medium doses （25, 50 mg/kg） against cerebral ischemia/reperfusion injury were weaker, while the protective effects at high dose （100 mg/kg） were similar. Our experimental findings suggest that TFLC exerts neuroprotective effects against focal cerebral ischemia/reperfusion injury in rats, and that the effects may be asso- ciated with its antioxidant activities.

High Frequency Sporophytes Regeneration from the Spore Culture of the Endangered Aquatic Fern <i>Isoetes coreana</i>

Using a mixed culture of megaspores and microspores from I. coreana, we established high frequency sporophyte regeneration system. After 20 days of culturing in MS basal medium, microscopic examination showed significant morphological changes and the microspore released numerous small vesicles into the culture medium. Megaspores also showed dramatic morphological changes during its incubation time in culture. The spore wall was cracked by the expansion of the megaspore (about 2 times increase in diameter). Simultaneously, brown spots were observed on the surface of the megaspores. The frequency of female gametophytes developing from immature megaspores cultured in MS basal liquid medium (pH 7) supplemented with 1 mgl-1 GA3 was 46%. However, these female gametophytes derived from megaspore only culture could not differentiate into sporophytes. The mixed culture of microspores and megaspores resulted in successful sporophyte regeneration. The highest frequency (12.3%) of green sporophyte regeneration from mixed spore culture occurred when the cultures were maintained at 25℃ under cool-white fluorescent light (40 μmol·m-2·s-1) with a 16 h photoperiod. Regenerated sporophytes were transferred to a test tube containing vermiculite and a sand mixture and left there until they had three leaves. After root growth and the fifth leaf had emerged, more than 95% of the regenerated sporophytes were successfully transferred to the soil and grown to mature plants. The sporophyte regeneration system established in this study could be successfully used for the restoration of the endangered aquatic species, I. coreana.

北苍术与朝鲜苍术健脾作用及机制研究

目的 分析北苍术与朝鲜苍术干预后,脾虚大鼠血浆生物标志物及代谢通路的差异,从非靶向代谢组学的角度讨论两者的健脾机制。方法 将SD大鼠采用饮食不节、疲劳过度加苦寒泻下的复合因素法,造成脾虚症模型。测定胃肠道和免疫学指标的水平;采用超高效液相色谱串联Q Exactive质谱仪(UHPLC-QE-MS)技术,联合主成分分析(PCA)与正交偏最小二乘法判别分析(OPLS-DA)方法,寻找脾虚大鼠血浆中生物标志物;利用Pathway数据库归纳代谢通路。结果 经北苍术与朝鲜苍术给药后,脾虚大鼠血浆中各指标均有不同程度回调。代谢组学分析表明,北苍术与朝鲜苍术分别回调了70个和82个血浆差异代谢物,北苍术主要调节“甘氨酸、丝氨酸和苏氨酸代谢”与“硫胺素代谢”2个代谢通路,朝鲜苍术主要调节“甘氨酸、丝氨酸和苏氨酸代谢”、“硫胺素代谢”、“嘧啶代谢”、“丁酸代谢”与“核黄素代谢”5个代谢通路。结论 北苍术与朝鲜苍术对脾虚大鼠均具有一定的调节作用,其作用机制可能与调节“甘氨酸、丝氨酸和苏氨酸代谢”、“硫胺素代谢”等代谢通路有关。

朝鲜苍术对脾虚大鼠的药理学影响

目的:明确朝鲜苍术对脾虚大鼠的药理学指标影响,以北苍术作为阳性药,并就实验结果对比阳性药组对朝鲜苍术的健脾作用进行评价,为朝鲜苍术更合理的使用提供实验依据。方法:将40只雄性SD大鼠随机分为空白组、模型组、阳性药组、朝鲜苍术组四组,每组10只。用空白组外的3组采用饥饱失常、疲劳过度加苦寒泻下的复合因素法造模21天建立脾虚模型。造模第22-28天,阳性药组与朝鲜苍术组大鼠分别用等剂量的北苍术与朝鲜苍术给药干预。称量各组大鼠的体质量、胸腺质量和脾脏质量,计算胸腺指数和脾脏指数,酶联免疫吸附法(ELISA)测定各组大鼠血清中淀粉酶(AMS)、胃泌素(GAS)、胃动素(MTL)、胆囊收缩素(CCK)、饥饿素(Ghrelin)、生长抑素(SS)、免疫球蛋白G(IgG)、干细胞因子(SCF)、白细胞介素4(IL-4)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)、γ干扰素(IFN-γ)、血管活性肠肽(VIP)、表皮生长因子(EGF)及三叶因子1(TFF1)的水平变化情况。结果:与空白组相比较,模型组大鼠的体质量、胸腺指数、脾脏指数显著下降(P<0.05),血清中AMS、IFN-γ、IgG、IL-4和TFF1水平显著下降(P<0.05),CCK、GAS、Ghrelin、MTL、SS、VIP、SCF、EGF、IL-6和TNF-α水平显著上升(P<0.05)。与模型组相比较,阳性药组大鼠血清中SS和IL-4水平显著回调(P<0.05),IL-6水平未呈现差异性;朝鲜苍术组大鼠血清中,除SS水平呈现回调趋势但未呈现显著性,IL-4和IL-6水平未表现出差异性外,其余各组指标皆呈现回调趋势,其趋势均呈现显著性(P<0.05)。与阳性药组相比较,朝鲜苍术组大鼠血清中MTL、EGF、IFN-γ、TNF-α水平显著升高(P<0.05),CCK、AMS、Ghrelin、IgG水平显著降低(P<0.05)。结论:阳性药与朝鲜苍术对脾虚都具有良好的调节作用,在大鼠脾虚引发的体质量变化以及胸腺、脾脏和代偿机制的改善方面,朝鲜苍术表现更优;在对脾虚大鼠的免疫调节、炎症反应调节以及溃疡的修复方面,阳性药表现更优。

安庆市酸奶消费现状与老鹰茶酸奶接受度评价研究

本文采用问卷调查法和感官评价法对安庆市酸奶消费现状与老鹰茶酸奶接受度进行评估分析。结果显示,安庆市消费者饮用酸奶频率较高,产品品质特性、品牌和价格是影响酸奶品种选择的重要因素。志愿者评价老鹰茶酸奶具有酸奶典型的滋味和老鹰茶独特的风味,56.5%的志愿者认为老鹰茶酸奶应具有良好的抗氧化活性。安庆酸奶市场前景好,消费者对功能酸奶和营养强化型酸奶的认可度较高,老鹰茶酸奶被志愿者所喜欢。

豹皮樟总黄酮对免疫低下小鼠免疫功能的影响

目的研究豹皮樟总黄酮(litsea coteana total flavone,LCTF)对环磷酰胺(cyclophosphamide,Cy)诱导的免疫低下小鼠免疫功能的影响。方法以Cy50mg·kg-1ip给药,每天1次,连续2d,诱导小鼠免疫低下模型,采用碳廓清试验、溶血素生成试验、二硝基氟苯(DNFB)诱导的迟发性超敏反应,观察LCTF对免疫低下小鼠非特异性免疫、体液免疫和细胞免疫的影响。结果碳廓清试验,LCTF(200、400mg·kg-1)组可提高Cy致免疫低下小鼠的吞噬指数α值和廓清指数K值,提高免疫低下小鼠巨噬细胞的吞噬功能;溶血素试验,LCTF(100、200mg·kg-1)组能提高免疫低下小鼠血清IgM和IgG的生成,LCTF(100、200、400mg·kg-1)能增加免疫低下小鼠脾细胞溶血素的产生;LCTF(200、400mg·kg-1)组可明显促进免疫低下小鼠DTH反应,LCTF(100、200、400mg·kg-1)能提升CD4+、CD8+细胞数以及CD4+/CD8+比值,并能提高脾淋巴细胞生产IL-2含量。结论以上结果表明,LCTF通过广泛刺激小鼠的特异性及非特异性免疫应答,对免疫低下小鼠的免疫功能有良好的增强作用。

豹皮樟总黄酮对大鼠佐剂性关节炎的作用及部分机制研究

目的研究豹皮樟总黄酮(total flavonoids of Litseacoreana Leve,TFLC)对佐剂性关节炎的治疗作用及其机制。方法采用福氏完全佐剂诱导大鼠佐剂性关节炎(adjuvant-induced arthritis,AA)模型,观察TFLC的抗炎作用;放免法测定AA大鼠腹腔巨噬细胞(peritoneal macrophages,PMΦ)中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、白细胞介素6(interleukin-6,IL-6)的含量;MTT法检测AA大鼠脾淋巴细胞增殖反应;HE染色法观察AA大鼠膝关节病理组织学变化;免疫组化法观察AA大鼠膝关节滑膜细胞中基质金属蛋白酶9(matrix metalloproteinases,MMP-9)蛋白表达情况。结果TFLC(50、100、200mg.kg-1)对AA大鼠的原发性、继发性炎症具有明显的抑制作用,降低多发性关节炎评分;TFLC(50、100、200mg.kg-1)可提高AA大鼠低下的脾淋巴细胞增殖反应;TFLC(50、100、200mg.kg-1)有效降低AA大鼠PMΦ产生的TNF-α、IL-6水平;TFLC(50、100、200mg.kg-1)改善AA大鼠膝关节病理损伤;TFLC(50、100、200mg.kg-1)能明显降低AA大鼠膝关节滑膜细胞中MMP-9的蛋白表达。结论TFLC(50、100、200mg.kg-1)对AA大鼠具有明显的治疗作用,抗炎机制可能与调节免疫功能、减少细胞因子的生成以及抑制基质金属蛋白酶的蛋白表达有关。

豹皮樟总黄酮对大鼠非酒精性脂肪性肝炎的防治作用

目的研究豹皮樟总黄酮(TFLC)对大鼠非酒精性脂肪性肝炎(NASH)的防治作用。方法SD大鼠48只,随机分为6组(n=8):对照组、模型组、烟酸组及TFLC组(50、100、200 mg.kg-1)。除对照组外,其余各组每天给予脂肪乳剂和相应药物。实验开始后3 d收集72 h的粪便,测定粪便中脂质;于1 wk后断尾取血,测定血清脂质;3 wk后处死动物,测定血清甘油三酯(TG)、总胆固醇(TC)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、丙二醛(MDA)、超氧化物歧化酶(SOD)和肝匀浆TG、TC、MDA、SOD,并行肝脏病理学检查。结果模型组大鼠出现NASH,烟酸组及TFLC组(100、200 mg.kg-1)均能降低血清和肝脏TG,肝脂肪变性程度和炎症均明显减轻;TFLC组(200 mg.kg-1)还能降低血清和肝MDA,提高肝组织SOD活性。结论TFLC可以降低大鼠血和肝组织TG,且能改善NASH大鼠脂肪变,减轻炎症反应。其机制可能是:TFLC抑制TG在肠道的吸收,同时减轻脂质过氧化。

豹皮樟总黄酮对胶原性关节炎大鼠腹腔巨噬细胞产生细胞因子及其免疫功能的影响

目的研究豹皮樟总黄酮(total flavonoids of LitseacoreanaLeve,TFLC)对胶原性关节炎大鼠(collagen-inducedarthritis,CIA)腹腔巨噬细胞(peritoneal macrophage,PMΦ)产生细胞因子及其免疫功能的影响。方法Ⅱ型胶原蛋白(collagenⅡ,CII)和氟氏完全佐剂(CFA)诱导大鼠胶原性关节炎(CIA)模型,采用体重、足爪肿胀及病理组织学变化等指标评价TFLC对CIA大鼠的治疗作用;无菌取PMΦ,体外用药(TFLC0.05、0.5、5mg·mL-1),采用小鼠胸腺细胞增殖法检测CIA大鼠PMΦ分泌白细胞介素1(interleukin-1,IL-1)的水平,小鼠脾淋巴细胞增殖法检测CIA大鼠脾细胞分泌白细胞介素2(interleukin-2,IL-2)的水平,放射免疫测定法和反转录RT-PCR法分别检测CIA大鼠PMΦ中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)的分泌和表达,中性红法测定CIA大鼠PMΦ的吞噬功能。结果TFLC(50、100、200mg.kg-1)能抑制关节肿胀,增加CIA大鼠体重,改善膝关节病理损伤。TFLC(0.05、0.5、5mg·mL-1)可降低CIA大鼠PMΦ过强的吞噬功能,下调脾细胞IL-2的分泌水平和腹腔巨噬细胞IL-1的分泌水平,同时抑制PMΦTNF-α的分泌水平以及TNF-αmRNA的表达。结论TFLC对CIA大鼠关节炎具有明显抑制作用,其抗炎和免疫抑制作用与其降低CIA大鼠PMΦ过强的吞噬功能,抑制细胞因子的分泌和表达有关。

NaHCO_3胁迫下朝鲜接骨木和茶条槭苗木的生长及生理响应

采用盆栽试验的方法研究不同浓度NaHCO3(对照,1.0,2.0,4.0g·kg-1)胁迫下1年生朝鲜接骨木和茶条槭苗木的生长和生理生化指标的变化。结果显示:2树种苗木对低浓度的NaHCO3胁迫适应性较好,中、高浓度的NaHCO3胁迫下成活和生长受到明显抑制。在低、中、高处理下朝鲜接骨木存活率分别为100%,90%和65%,茶条槭存活率分别为100%,75%和55%。盐胁迫强度和时间均对2树种苗木的生理指标产生明显影响。随着盐胁迫浓度的增加和胁迫时间的延长,2树种苗木的MDA含量和细胞膜相对透性均显著升高,ASA和GSH含量呈上升趋势,脯氨酸和可溶性糖也大量积累。朝鲜接骨木在胁迫中期各处理GSH含量较对照分别增加68.5%,54.5%,90.4%,茶条槭在胁迫后期GSH含量较对照分别增加87.9%,124.9%,96.9%。朝鲜接骨木可溶性糖含量在胁迫后期各处理较对照分别增加46.7%,38.3%,51.9%;茶条槭分别增加15.6%,31.8%,22.7%。研究结果表明:2树种在低浓度盐胁迫环境下相关生理指标相对稳定,具有一定的抗性,可耐受的土壤NaHCO3的含量为1.0g·kg-1,朝鲜接骨木对碳酸盐的抗性略高于茶条槭。

温度对产虫茶昆虫紫斑谷螟生长发育的影响

为探明温度对贵州主要产虫茶昆虫紫斑谷螟Pyralis farinalis(Linnaeus)生长发育的影响,本研究以白茶Litsea coreana为寄主植物,分别设置5个恒温(19,22,25,28和31℃)条件,研究温度对紫斑谷螟卵、幼虫、蛹和未成熟期平均发育历期、发育速率和存活率的影响,计算各虫态发育起点温度和有效积温。结果表明:温度对紫斑谷螟各虫态发育历期、发育速率和存活率影响显著。在19～31℃范围内,各虫态的平均发育历期均随温度的升高而缩短,卵期、幼虫期、蛹期及未成熟期均在31℃达到最小值,分别为4.56±0.24,43.33±1.50,7.89±0.20和55.78±1.69d。紫斑谷螟各虫态发育速率与温度呈二次回归关系,且极显著相关。此外,温度显著影响各虫态存活率,卵的存活率在28℃时最高,为93%;幼虫和蛹的存活率则在25℃最高,分别为88%和93%;温度过高或过低均不利于其生长发育。由直接最优法计算得到紫斑谷螟卵期、幼虫期、蛹期及未成熟期的发育起点温度分别为13.30,15.48,13.19和14.82℃,有效积温依次为88.36,679.51,159.73和952.04日·度。这些结果为紫斑谷螟的繁殖提供了基础参考数据,对指导虫茶生产有实用参考价值。

老鹰茶正丁醇部分的化学成分

目的对樟科植物老鹰茶叶醇提取物中正丁醇部分的化学成分进行研究。方法老鹰茶经70%乙醇提取,再经石油醚、氯仿、乙酸乙酯和正丁醇依次萃取,正丁醇部分用大孔树脂、硅胶、Sephadex LH-20以及制备高效液相色谱法等进行分离纯化,通过1H-NMR、13C-NMR、MS等方法鉴定化合物的结构。结果从老鹰茶叶的正丁醇部分分离得到7个化合物,分别鉴定为原儿茶酸、山奈酚-3-O-β-D-(6-O-trans-p-香豆酰基)吡喃葡萄糖苷、山奈酚-3-O-β-D-(6-O-trans-p-香豆酰基)吡喃甘露糖苷、山奈酚-3-O-α-L-鼠李糖苷、反式香豆酸、邻苯二甲酸二正丁酯、水杨酸。结论这7种化合物均为首次从该植物中分离得到。

贵州老鹰茶(豹皮樟)挥发油成分研究

利用常压水蒸气蒸馏提取贵州老鹰茶 (Litsea coreana Levl.Var.Lanuginose)的挥发性成分，挥发油得率 0.1％，利用 GC－ MS技术对老鹰茶的挥发油进行了研究，从 Litsea coreana挥发油中鉴定出 32种成分，占挥发油组成的 95％，主要成分是正癸醛 (Decanal)含量 71.53％；其次是 10－烯－十—醛 (10－ undecenal)，含量 5.02％；壬醛 (n－ nonaldehyde)，含量 3.95％； copaene，含量 3.58％，正十二酸乙烯酯 (Dodecanoic acid,ethenyl ester)，含量 2.63％。

不同工艺提取老鹰茶总黄酮含量的比较

比较不同工艺提取富集的老鹰茶中总黄酮含量,分别采用传统乙醇提取-正丁醇萃取法和水提取-大孔树脂法提取老鹰茶总黄酮,并用紫外分光光度法,以芦丁为标准品,270nm下测定总黄酮含量。水提取-大孔树脂法富集所得固体中黄酮含量较高,可达55.86%,回收率为105.6%,RSD=1.64%。水提取-大孔树脂富集的工艺操作简单,所得固体黄酮含量高,更适于老鹰茶总黄酮的提取,对进一步的工业化生产开发有较好的参考价值。

老鹰茶总黄酮的提取工艺及抗氧化活性研究

以老鹰茶为原料,槲皮素为对照品,总黄酮含量为考核指标,研究了老鹰茶总黄酮的水浴提取工艺,同时对总黄酮提取物的抗氧化活性进行了探讨。在提取温度、时间、溶剂浓度、料液比等单因素的基础上,通过正交实验,确定老鹰茶总黄酮的最佳工艺条件为:提取温度60℃,提取时间90min,乙醇浓度70%,液固比35∶1。在此条件下,测得的老鹰茶总黄酮得率为6.190%(以槲皮素计)。老鹰茶总黄酮的抗氧化活性结果表明,老鹰茶总黄酮对DPPH自由基具有较强的清除活性,其EC50值为18.17μg/mL;老鹰茶总黄酮具有较强的还原能力,其EC50值为77.52μg/mL。老鹰茶总黄酮具有较强的抗氧化活性,是一种潜在的具有抗氧化功能食品原料。

老鹰茶中三种黄酮类物质抗脂质过氧化作用初探

本文采用组织匀浆MDA比色法测定了山柰酚、山柰酚 3 O β D 葡萄糖甙、槲皮素 3 O β D 葡萄糖甙三种贵州老鹰茶 (Litseacoreana)黄酮类提取物对大鼠离体心、脑、肝、肾四种组织脂质过氧化的影响 ,发现以上三种化合物均具有明显的抗氧化活性 ,并呈量效关系 ,且抗氧化活性为 :山柰酚 >槲皮素 3 O β D 葡萄糖甙 >山柰酚 3 O β D 葡萄糖甙 ;以上三种物质对SD大鼠四种离体组织MDA抑制率为 :肾组织 >心组织 >肝组织 >脑组织。本文的研究结果为开发和利用贵州老鹰茶叶中黄酮类天然抗氧化剂提供了一定的科学依据。