Corn steep liquor(CSL) is an important raw material that has high nutritional value and serves as a nitrogen source.Biotin in CSL is especially of great importance to fermentation.In order to develop a fast,versatile,...Corn steep liquor(CSL) is an important raw material that has high nutritional value and serves as a nitrogen source.Biotin in CSL is especially of great importance to fermentation.In order to develop a fast,versatile,cheap,and environmentally safe analytical method for quantifying vitamins B2(VB2),B3(VB3),B6(VB6) and B7(VB7) in CSL,the near-infrared spectroscopy(NIR) measurements of 66 samples(22 batches) of CSL were analyzed by partial least-square regression(PLSR).Multivariate models developed in the NIR regions showed good predictive abilities for VB2,VB3,VB6 and VB7.Results confirmed the probability of the multivariate spectroscopic approach as a replacement for expensive and time-consuming conventional chemical methods.展开更多
This study was to determine the fermentation quality of a mixture of corn steep liquor(CSL)(178 g/kg wet basis) and air-dried rice straw(356 g/kg wet basis) after being treated with inoculants of different types of la...This study was to determine the fermentation quality of a mixture of corn steep liquor(CSL)(178 g/kg wet basis) and air-dried rice straw(356 g/kg wet basis) after being treated with inoculants of different types of lactic acid bacteria(LAB). The treatments included the addition of no LAB additive(control),which was deionized water; homo-fermentative LAB alone(^(ho)LAB), which was Lactobacillus plantarum alone), and a mixture of homo-fermentative and hetero-fermentative LAB(^(he+ho)LAB), which were L. plantarum, Lactobacillus casei, and Lactobacillus buchneri. The results showed that the inoculation of the mixture of CSL and air-dried rice straw with ^(he+ho)LAB significantly increased the concentration of acetic acid and lactic acid compared with the control(P < 0.05). The addition of ^(he+ho)LAB effectively inhibited the growth of yeast in the silage. The concentration of total lactic acid bacteria in the ^(he+ho)LAB-treated silage was significant higher than those obtained in other groups(P < 0.05). The duration of the aerobic stability of the silages increased from 56 h to >372 h. The control group was the first to spoil, whereas the silage treated with ^(he+ho)LAB remained stable throughout the 372 h period of monitoring. The results demonstrated that the ^(he+ho)LAB could effectively improve the fermentation quality and aerobic stability of the silage.展开更多
The object of this study was to determine the proper mixing ratio of fresh rice straw to corn steep liquor(CSL) to obtain a high protein content silage feed. The following experimental silages were generated: the cont...The object of this study was to determine the proper mixing ratio of fresh rice straw to corn steep liquor(CSL) to obtain a high protein content silage feed. The following experimental silages were generated: the control(C1), composed of fresh rice straw without CSL additive, mixed with CSL in the ratios of 4:1(C4),3:1(C3) and 2:1(C2). Lactic acid bacteria(LAB) inoculant was applied at the rate of 50 mL/kg(fresh basis)of forage to achieve a final application rate of 1 x 10~6 cfu/g of fresh matter(FM). Duplicate silos for each treatment were opened after 0,3, 7,10,20,30,45 and 60 d for microbiological and chemical analysis. The results showed that the addition of CSL significantly increased crude protein(CP) contents, and decreased neutral detergent fiber(NDF) and acid detergent fiber(ADF) contents of treatments after 60 d of ensiling(P < 0.05). The lactic acid contents in C4 and C3 were significantly higher than that in C1(P <0.05). In summary, mixing fresh rice straw with CSL at addition levels of 4:1(C4) and 3:1(C3) can improve the fermentation quality and nutrient composition of fresh rice straw silage. However, a large proportion of CSL(C3) had a negative impact on the aerobic stability of fresh rice straw.展开更多
基金Supported by Foundation of Tianjin City Science and Technology Project (No.09ZCKFSH00900)
文摘Corn steep liquor(CSL) is an important raw material that has high nutritional value and serves as a nitrogen source.Biotin in CSL is especially of great importance to fermentation.In order to develop a fast,versatile,cheap,and environmentally safe analytical method for quantifying vitamins B2(VB2),B3(VB3),B6(VB6) and B7(VB7) in CSL,the near-infrared spectroscopy(NIR) measurements of 66 samples(22 batches) of CSL were analyzed by partial least-square regression(PLSR).Multivariate models developed in the NIR regions showed good predictive abilities for VB2,VB3,VB6 and VB7.Results confirmed the probability of the multivariate spectroscopic approach as a replacement for expensive and time-consuming conventional chemical methods.
基金financially supported by the National Dairy Industry and Technology System project (CARS-37)of Agriculture Ministry in China
文摘This study was to determine the fermentation quality of a mixture of corn steep liquor(CSL)(178 g/kg wet basis) and air-dried rice straw(356 g/kg wet basis) after being treated with inoculants of different types of lactic acid bacteria(LAB). The treatments included the addition of no LAB additive(control),which was deionized water; homo-fermentative LAB alone(^(ho)LAB), which was Lactobacillus plantarum alone), and a mixture of homo-fermentative and hetero-fermentative LAB(^(he+ho)LAB), which were L. plantarum, Lactobacillus casei, and Lactobacillus buchneri. The results showed that the inoculation of the mixture of CSL and air-dried rice straw with ^(he+ho)LAB significantly increased the concentration of acetic acid and lactic acid compared with the control(P < 0.05). The addition of ^(he+ho)LAB effectively inhibited the growth of yeast in the silage. The concentration of total lactic acid bacteria in the ^(he+ho)LAB-treated silage was significant higher than those obtained in other groups(P < 0.05). The duration of the aerobic stability of the silages increased from 56 h to >372 h. The control group was the first to spoil, whereas the silage treated with ^(he+ho)LAB remained stable throughout the 372 h period of monitoring. The results demonstrated that the ^(he+ho)LAB could effectively improve the fermentation quality and aerobic stability of the silage.
基金funded by the National Dairy Industry and Technology System project (CARS-37)of Agriculture Ministry in China
文摘The object of this study was to determine the proper mixing ratio of fresh rice straw to corn steep liquor(CSL) to obtain a high protein content silage feed. The following experimental silages were generated: the control(C1), composed of fresh rice straw without CSL additive, mixed with CSL in the ratios of 4:1(C4),3:1(C3) and 2:1(C2). Lactic acid bacteria(LAB) inoculant was applied at the rate of 50 mL/kg(fresh basis)of forage to achieve a final application rate of 1 x 10~6 cfu/g of fresh matter(FM). Duplicate silos for each treatment were opened after 0,3, 7,10,20,30,45 and 60 d for microbiological and chemical analysis. The results showed that the addition of CSL significantly increased crude protein(CP) contents, and decreased neutral detergent fiber(NDF) and acid detergent fiber(ADF) contents of treatments after 60 d of ensiling(P < 0.05). The lactic acid contents in C4 and C3 were significantly higher than that in C1(P <0.05). In summary, mixing fresh rice straw with CSL at addition levels of 4:1(C4) and 3:1(C3) can improve the fermentation quality and nutrient composition of fresh rice straw silage. However, a large proportion of CSL(C3) had a negative impact on the aerobic stability of fresh rice straw.
