目的基于网络药理学探究山茱萸-附子治疗心力衰竭的分子机制。方法2021年4月至2022年5月,运用中药系统药理学数据库和分析平台(TCMSP)数据库对山茱萸-附子有效成分进行筛选,利用SwissTargetPrediction数据库进行靶点预测。通过DisGeNet...目的基于网络药理学探究山茱萸-附子治疗心力衰竭的分子机制。方法2021年4月至2022年5月,运用中药系统药理学数据库和分析平台(TCMSP)数据库对山茱萸-附子有效成分进行筛选,利用SwissTargetPrediction数据库进行靶点预测。通过DisGeNet、DrugBank、OMIM疾病数据库收集心力衰竭的治疗靶点,取疾病与药物之间的靶点交集,将靶点交集导入STRING11.5数据库构建山茱萸-附子蛋白质-蛋白质相互作用(proteinprotein interaction,PPI)网络图。利用DAVID数据库对关键靶点进行基于京都基因和基因组数据库(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析,使用AutoDuckTool 1.5.6软件对关键靶点进行分子对接。结果筛选出山茱萸-附子有效化学成分33种、靶点208个,心力衰竭疾病靶点2046个,山茱萸-附子治疗心力衰竭的靶点85个。在DAVID数据库中进行基因本体论(GO)分析得出与疾病最相关的前20个条目,KEGG分析显示81条通路。山茱萸-附子治疗心力衰竭的关键靶点有过氧化物酶体增殖物激活受体γ(PPARG)、表皮生长因子受体(EGFR)、雌激素受体1(ESR1)、前列腺素内过氧化物合酶2(PTGS2),KEGG通路主要有环磷酸鸟苷-蛋白激酶G信号通路(cGMP-PKG)、缺氧诱导因子1信号通路(HIF-1)、过氧化物酶体增殖物激活受体信号通路(PPAR)等;分子对接结果显示活性成分β-谷甾醇与关键靶点ESR1、EGFR的对接亲和力较好。结论山茱萸-附子通过多靶点、多成分、多通路的作用机制治疗心力衰竭。展开更多
The components of crude and processed Fructus Corni were investigated by means of electrospray ionization-tandem mass spectrometry（ESI-MSn） technique in the negative ion mode. Compared with those of crude Fructus Co...The components of crude and processed Fructus Corni were investigated by means of electrospray ionization-tandem mass spectrometry（ESI-MSn） technique in the negative ion mode. Compared with those of crude Fructus Corni, the chemical components of the processed Fructus Corni were changed both in quality and in quantity. From the ESI-MS spectra of the crude and processed Fructus Corni, six peaks were selected to establish the characte-ristic ESI-MS peaks. Several factors in the processing procedure were examined. The experimental results demonstrate that the chemical reactions that occurred in the processing procedure can be used for the elucidation of the processed mechanism of Fructus Corni, which is regularly affected by the processing conditions. The present article provides both the chemistry evidence for the understanding of the processing procedure of Fructus Corni and the specific methodology for the research of the processing procedure and quality identification of traditional Chinese medicine.展开更多
Zhang Xichun classifed the collapse syndrome into upper collapse,lower collapse and exterior col-lapse.Based on the therapeutic principle of tonifying the liver and astringing the liver to consolidate collapse,he used...Zhang Xichun classifed the collapse syndrome into upper collapse,lower collapse and exterior col-lapse.Based on the therapeutic principle of tonifying the liver and astringing the liver to consolidate collapse,he used drugs boldly to cure disease.The method of tonifying the liver and astringing sweating is applied to res-cue exterior collapse with Laifu Deoction(来复汤);the method of tonifying the liver and relieving asthma is applied to treat upper collapse with Shenzhe Zhenqi Decoction(参赭镇气汤);the method of restoring yang with emergent treatment is applied to rescue the lower collapse with Jijiu Huiyang Decoction(急救回阳汤).This will provide references for the prevention and treatment of critical diseases for later generations in practi-cing Chinese medicine.展开更多
文摘目的基于网络药理学探究山茱萸-附子治疗心力衰竭的分子机制。方法2021年4月至2022年5月,运用中药系统药理学数据库和分析平台(TCMSP)数据库对山茱萸-附子有效成分进行筛选,利用SwissTargetPrediction数据库进行靶点预测。通过DisGeNet、DrugBank、OMIM疾病数据库收集心力衰竭的治疗靶点,取疾病与药物之间的靶点交集,将靶点交集导入STRING11.5数据库构建山茱萸-附子蛋白质-蛋白质相互作用(proteinprotein interaction,PPI)网络图。利用DAVID数据库对关键靶点进行基于京都基因和基因组数据库(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析,使用AutoDuckTool 1.5.6软件对关键靶点进行分子对接。结果筛选出山茱萸-附子有效化学成分33种、靶点208个,心力衰竭疾病靶点2046个,山茱萸-附子治疗心力衰竭的靶点85个。在DAVID数据库中进行基因本体论(GO)分析得出与疾病最相关的前20个条目,KEGG分析显示81条通路。山茱萸-附子治疗心力衰竭的关键靶点有过氧化物酶体增殖物激活受体γ(PPARG)、表皮生长因子受体(EGFR)、雌激素受体1(ESR1)、前列腺素内过氧化物合酶2(PTGS2),KEGG通路主要有环磷酸鸟苷-蛋白激酶G信号通路(cGMP-PKG)、缺氧诱导因子1信号通路(HIF-1)、过氧化物酶体增殖物激活受体信号通路(PPAR)等;分子对接结果显示活性成分β-谷甾醇与关键靶点ESR1、EGFR的对接亲和力较好。结论山茱萸-附子通过多靶点、多成分、多通路的作用机制治疗心力衰竭。
基金the National Natural Science Foundation of China(No.30672600)the Great Research Project of Chinese Academy of Sciences(No.KGCX2-SW-213-06)+1 种基金the Natural Science Foundation of Jilin Province(No.20060902)the Natural Science Foundation of Changchun City, China(No.04-02GG233)
文摘The components of crude and processed Fructus Corni were investigated by means of electrospray ionization-tandem mass spectrometry（ESI-MSn） technique in the negative ion mode. Compared with those of crude Fructus Corni, the chemical components of the processed Fructus Corni were changed both in quality and in quantity. From the ESI-MS spectra of the crude and processed Fructus Corni, six peaks were selected to establish the characte-ristic ESI-MS peaks. Several factors in the processing procedure were examined. The experimental results demonstrate that the chemical reactions that occurred in the processing procedure can be used for the elucidation of the processed mechanism of Fructus Corni, which is regularly affected by the processing conditions. The present article provides both the chemistry evidence for the understanding of the processing procedure of Fructus Corni and the specific methodology for the research of the processing procedure and quality identification of traditional Chinese medicine.
基金We thank for the funding support from the Scientific Research Project of Hunan Provincial Department of Education(18C0378)。
文摘Zhang Xichun classifed the collapse syndrome into upper collapse,lower collapse and exterior col-lapse.Based on the therapeutic principle of tonifying the liver and astringing the liver to consolidate collapse,he used drugs boldly to cure disease.The method of tonifying the liver and astringing sweating is applied to res-cue exterior collapse with Laifu Deoction(来复汤);the method of tonifying the liver and relieving asthma is applied to treat upper collapse with Shenzhe Zhenqi Decoction(参赭镇气汤);the method of restoring yang with emergent treatment is applied to rescue the lower collapse with Jijiu Huiyang Decoction(急救回阳汤).This will provide references for the prevention and treatment of critical diseases for later generations in practi-cing Chinese medicine.
