Cotton(Gossypium hirsutum L.) fibers,one of the most important natural raw materials for the textile industry,are highly elongated trichomes from epidermal cells of cotton ovules.Among the longest plant cells ever cha...Cotton(Gossypium hirsutum L.) fibers,one of the most important natural raw materials for the textile industry,are highly elongated trichomes from epidermal cells of cotton ovules.Among the longest plant cells ever characterized,cotton fiber is an ideal system for studying plant cell elongation.展开更多
In this project,we aim to elucidate the molecular mechanism controlling initiation and elongation of tetraploid Gossypium hirsutum fiber cells by setting up a high throughput custom-designed
The regulatory mechanism that controls the sustained cotton fiber cell elongation is gradually being elucidated by coupling genome-wide transcriptome profiling with systematic biochemical and physiological studies.Ver...The regulatory mechanism that controls the sustained cotton fiber cell elongation is gradually being elucidated by coupling genome-wide transcriptome profiling with systematic biochemical and physiological studies.Very long chain fatty acids(VLCFA),H2O2,and several types of plant展开更多
A comparative proteomic analysis was applied to explore the mechanism of fiber cell development in cotton.Initially,an efficient protein preparation method was established for proteomic analysis
Cotton fiber development is a highly programmed process,involving a large number of genes.Through cDNA array and RT-PCR,we isolated a Gossypium hirsutum RD22-1 like gene(GhRDL1),
Transfer cells （TCs） are specialized cells exhibiting invaginated wall ingrowths （Wls）, thereby amplifying their plasma membrane surface area （PMSA） and hence the capacity to transport nutrients. However, it rem...Transfer cells （TCs） are specialized cells exhibiting invaginated wall ingrowths （Wls）, thereby amplifying their plasma membrane surface area （PMSA） and hence the capacity to transport nutrients. However, it remains unknown as to whether TCs play a role in biomass yield increase during evolution or domestication. Here, we examine this issue from a comparative evolutionary perspective. The cultivated tetraploid AD genome species of cotton and its A and D genome diploid progenitors displayed high, medium, and low seed and fiber biomass yield, respectively. In all three species, cells of the innermost layer of the seed coat juxtaposed to the filial tissues trans-differentiated to a TC morphology. Electron microscopic analyses revealed that these TCs are characterized by sequential formation of flange and reticulate Wls during the phase of rapid increase in seed biomass. Significantly, TCs from the tetraploid species developed substantially more flange and reticulate Wls and exhibited a higher degree of reticulate WI formation than their progenitors. Consequently, the estimated PMSA of TCs of the tetraploid species was about 4 and 70 times higher than that of TCs of the A and D genome progenitors, respectively, which correlates positively with seed and fiber biomass yield. Further, TCs with extensive Wls in the tetraploid species had much stronger expression of sucrose synthase, a key enzyme involved in TC Wl formation and function, than those from the A and D progenitors. The analyses provide a set of novel evidence that the development of TC Wls may play an important role in the increase of seed and fiber biomass yield through polyploidization during evolution.展开更多
The secondary cell wall in mature cotton fibers contains over 90%cellulose with low quantities of xylan and lignin.However,little is known regarding the regulation of secondary cell wall biosynthesis in cotton fibers....The secondary cell wall in mature cotton fibers contains over 90%cellulose with low quantities of xylan and lignin.However,little is known regarding the regulation of secondary cell wall biosynthesis in cotton fibers.In this study,we characterized an R2R3-MYB transcription factor,Gh MYB7,in cotton.Gh MYB7 is expressed at a high level in developing fibers and encodes a MYB protein that is targeted to the cell nucleus and has transcriptional activation activity.Ectopic expression of Gh MYB7 in Arabidopsis resulted in small,curled,dark green leaves and also led to shorter inflorescence stems.A cross-sectional assay of basal stems revealed that cell wall thickness of vessels and interfascicular fibers was higher in transgenic lines overexpressing Gh MYB7 than in the wild type.Constitutive expression of Gh MYB7 in Arabidopsis activated the expression of a suite of secondary cell wall biosynthesis-related genes(including some secondary cell wall-associated transcription factors),leading to the ectopic deposition of cellulose and lignin.The ectopic deposition of secondary cell walls may have been initiated before the cessation of cell expansion.Moreover,Gh MYB7 was capable of binding to the promoter regions of At SND1 and At Ces A4,suggesting that Gh MYB7 may function upstream of NAC transcription factors.Collectively,these findings suggest that Gh MYB7 is a potential transcriptional activator,which may participate in regulating secondary cell wall biosynthesis of cotton fibers.展开更多
文摘Cotton(Gossypium hirsutum L.) fibers,one of the most important natural raw materials for the textile industry,are highly elongated trichomes from epidermal cells of cotton ovules.Among the longest plant cells ever characterized,cotton fiber is an ideal system for studying plant cell elongation.
文摘In this project,we aim to elucidate the molecular mechanism controlling initiation and elongation of tetraploid Gossypium hirsutum fiber cells by setting up a high throughput custom-designed
文摘The regulatory mechanism that controls the sustained cotton fiber cell elongation is gradually being elucidated by coupling genome-wide transcriptome profiling with systematic biochemical and physiological studies.Very long chain fatty acids(VLCFA),H2O2,and several types of plant
文摘A comparative proteomic analysis was applied to explore the mechanism of fiber cell development in cotton.Initially,an efficient protein preparation method was established for proteomic analysis
文摘Cotton fiber development is a highly programmed process,involving a large number of genes.Through cDNA array and RT-PCR,we isolated a Gossypium hirsutum RD22-1 like gene(GhRDL1),
文摘Transfer cells （TCs） are specialized cells exhibiting invaginated wall ingrowths （Wls）, thereby amplifying their plasma membrane surface area （PMSA） and hence the capacity to transport nutrients. However, it remains unknown as to whether TCs play a role in biomass yield increase during evolution or domestication. Here, we examine this issue from a comparative evolutionary perspective. The cultivated tetraploid AD genome species of cotton and its A and D genome diploid progenitors displayed high, medium, and low seed and fiber biomass yield, respectively. In all three species, cells of the innermost layer of the seed coat juxtaposed to the filial tissues trans-differentiated to a TC morphology. Electron microscopic analyses revealed that these TCs are characterized by sequential formation of flange and reticulate Wls during the phase of rapid increase in seed biomass. Significantly, TCs from the tetraploid species developed substantially more flange and reticulate Wls and exhibited a higher degree of reticulate WI formation than their progenitors. Consequently, the estimated PMSA of TCs of the tetraploid species was about 4 and 70 times higher than that of TCs of the A and D genome progenitors, respectively, which correlates positively with seed and fiber biomass yield. Further, TCs with extensive Wls in the tetraploid species had much stronger expression of sucrose synthase, a key enzyme involved in TC Wl formation and function, than those from the A and D progenitors. The analyses provide a set of novel evidence that the development of TC Wls may play an important role in the increase of seed and fiber biomass yield through polyploidization during evolution.
基金the National Natural Science Foundation of China (31371234)the project from the Ministry of Agriculture of China for transgenic research (2014ZX08009-27B)
文摘The secondary cell wall in mature cotton fibers contains over 90%cellulose with low quantities of xylan and lignin.However,little is known regarding the regulation of secondary cell wall biosynthesis in cotton fibers.In this study,we characterized an R2R3-MYB transcription factor,Gh MYB7,in cotton.Gh MYB7 is expressed at a high level in developing fibers and encodes a MYB protein that is targeted to the cell nucleus and has transcriptional activation activity.Ectopic expression of Gh MYB7 in Arabidopsis resulted in small,curled,dark green leaves and also led to shorter inflorescence stems.A cross-sectional assay of basal stems revealed that cell wall thickness of vessels and interfascicular fibers was higher in transgenic lines overexpressing Gh MYB7 than in the wild type.Constitutive expression of Gh MYB7 in Arabidopsis activated the expression of a suite of secondary cell wall biosynthesis-related genes(including some secondary cell wall-associated transcription factors),leading to the ectopic deposition of cellulose and lignin.The ectopic deposition of secondary cell walls may have been initiated before the cessation of cell expansion.Moreover,Gh MYB7 was capable of binding to the promoter regions of At SND1 and At Ces A4,suggesting that Gh MYB7 may function upstream of NAC transcription factors.Collectively,these findings suggest that Gh MYB7 is a potential transcriptional activator,which may participate in regulating secondary cell wall biosynthesis of cotton fibers.
