Fluorescence lifetime imaging microscopy(FLIM)is increasingly used in biomedicine,material science,chemistry,and other related research fields,because of its advantages of high specificity and sensitivity in monitorin...Fluorescence lifetime imaging microscopy(FLIM)is increasingly used in biomedicine,material science,chemistry,and other related research fields,because of its advantages of high specificity and sensitivity in monitoring cellular microenvironments,studying interaction between proteins,metabolic state,screening drugs and analyzing their efficacy,characterizing novel materials,and diagnosing early cancers.Understandably,there is a large interest in obtaining FLIM data within an acquisition time as short as possible.Consequently,there is currently a technology that advances towards faster and faster FLIM recording.However,the maximum speed of a recording technique is only part of the problerm.The acquisition time of a FLIM image is a complex function of many factors.These include the photon rate that can be obtained from the sample,the amount of information a technique extracts from the decay functions,the fficiency at which it determines fluorescence decay parameters from the recorded photons,the demands for the accuracy of these parameters,the number of pixels,and the lateral and axial resolutions that are obtained in biological materials.Starting from a discussion of the parameters which determine the acquisition time,this review will describe existing and emerging FLIM techniques and data analysis algo-rithms,and analyze their performance and recording speed in biological and biomedical applications.展开更多
[目的/意义]基于论文和期刊视角分析Web of Science(Wo S)数据库中不同引证时间窗口论文使用次数与被引频次之间的关系以及量引背离现象。[方法/过程]收集2013~2015年Wo S数据库收录眼科学领域被引频次TOP10%论文数据,分析文献类型,不...[目的/意义]基于论文和期刊视角分析Web of Science(Wo S)数据库中不同引证时间窗口论文使用次数与被引频次之间的关系以及量引背离现象。[方法/过程]收集2013~2015年Wo S数据库收录眼科学领域被引频次TOP10%论文数据,分析文献类型,不同引证时间窗口论文使用次数、被引频次的变化,从论文和期刊视角分析论文被引频次与使用次数的相关性,并对量引背离现象进行分析。[结果/结论]TOP10%论文主要文献类型为Article。随论文被引频次升高,使用次数(180天,U1)、使用次数(2013年至今U2)逐渐升高。被引频次、U2随引证时间窗口增加而升高(均为P<0.05),U1表现相反(均为P<0.05);不同引证时间窗口论文U1、U2和被引频次,以及期刊篇均被引频次和篇均U1、篇均U2均有相关性(均为P<0.05)。少数论文出现量引背离现象。揭示了高影响力论文的主要文献类型,也提示了论文使用与被引之间的共性,共性大小与引文时间窗口有关。引文时间窗口越长,论文总被引频次和总使用次数越高,最近180天内学者们更倾向于使用较新的文献,高使用次数预示着一定时期后的高被引。展开更多
基金support from the National Key R&D Program of China(2017YFA0700500)National Natural Science Foundation of China(61775144/61525503/61620106016/61835009/81727804)+2 种基金(Key)Project of Department of Education of Guangdong Province(2015KGJHZ002/2016KCXTD007)Guangdong Natural Science Foundation(2014A030312008,2017A030310132,2018A030313362)Shenzhen Basic Research Project(JCYJ20170818144012025/JCYJ20170818141701667/JCYJ20170412105003520/JCYJ20150930104948169).
文摘Fluorescence lifetime imaging microscopy(FLIM)is increasingly used in biomedicine,material science,chemistry,and other related research fields,because of its advantages of high specificity and sensitivity in monitoring cellular microenvironments,studying interaction between proteins,metabolic state,screening drugs and analyzing their efficacy,characterizing novel materials,and diagnosing early cancers.Understandably,there is a large interest in obtaining FLIM data within an acquisition time as short as possible.Consequently,there is currently a technology that advances towards faster and faster FLIM recording.However,the maximum speed of a recording technique is only part of the problerm.The acquisition time of a FLIM image is a complex function of many factors.These include the photon rate that can be obtained from the sample,the amount of information a technique extracts from the decay functions,the fficiency at which it determines fluorescence decay parameters from the recorded photons,the demands for the accuracy of these parameters,the number of pixels,and the lateral and axial resolutions that are obtained in biological materials.Starting from a discussion of the parameters which determine the acquisition time,this review will describe existing and emerging FLIM techniques and data analysis algo-rithms,and analyze their performance and recording speed in biological and biomedical applications.
文摘[目的/意义]基于论文和期刊视角分析Web of Science(Wo S)数据库中不同引证时间窗口论文使用次数与被引频次之间的关系以及量引背离现象。[方法/过程]收集2013~2015年Wo S数据库收录眼科学领域被引频次TOP10%论文数据,分析文献类型,不同引证时间窗口论文使用次数、被引频次的变化,从论文和期刊视角分析论文被引频次与使用次数的相关性,并对量引背离现象进行分析。[结果/结论]TOP10%论文主要文献类型为Article。随论文被引频次升高,使用次数(180天,U1)、使用次数(2013年至今U2)逐渐升高。被引频次、U2随引证时间窗口增加而升高(均为P<0.05),U1表现相反(均为P<0.05);不同引证时间窗口论文U1、U2和被引频次,以及期刊篇均被引频次和篇均U1、篇均U2均有相关性(均为P<0.05)。少数论文出现量引背离现象。揭示了高影响力论文的主要文献类型,也提示了论文使用与被引之间的共性,共性大小与引文时间窗口有关。引文时间窗口越长,论文总被引频次和总使用次数越高,最近180天内学者们更倾向于使用较新的文献,高使用次数预示着一定时期后的高被引。
