Kinetic equations are derived for the substrate reaction during simultaneous irreversible modification of enzyme activity for systems using coupled enzyme assay. It has been shown that the method proposed previously b...Kinetic equations are derived for the substrate reaction during simultaneous irreversible modification of enzyme activity for systems using coupled enzyme assay. It has been shown that the method proposed previously by Tsou for determining the irreversible inhibition constants can also be used in the present situation. Moreover, the criteria proposed to distinguish between different inhibition types are also applicable.展开更多
Following the previous paperr, we examined the kinetics of the irreversible modification of enzyme activity in coupled assay when the modifier reacted with both primary and auxiliary enzymes. It was shown that Tsou’s...Following the previous paperr, we examined the kinetics of the irreversible modification of enzyme activity in coupled assay when the modifier reacted with both primary and auxiliary enzymes. It was shown that Tsou’s kinetic method can also be used in the present situation in some conditions.展开更多
基金Project supported in part by the National Natural Science Foundation of China.
文摘Kinetic equations are derived for the substrate reaction during simultaneous irreversible modification of enzyme activity for systems using coupled enzyme assay. It has been shown that the method proposed previously by Tsou for determining the irreversible inhibition constants can also be used in the present situation. Moreover, the criteria proposed to distinguish between different inhibition types are also applicable.
文摘Following the previous paperr, we examined the kinetics of the irreversible modification of enzyme activity in coupled assay when the modifier reacted with both primary and auxiliary enzymes. It was shown that Tsou’s kinetic method can also be used in the present situation in some conditions.
