Coxsackie virus A16(CA16) is commonly recognized as one of the main human pathogens of hand-foot-mouth disease(HFMD). The clinical manifestations of HFMD include vesicles of hand, foot and mouth in young children and ...Coxsackie virus A16(CA16) is commonly recognized as one of the main human pathogens of hand-foot-mouth disease(HFMD). The clinical manifestations of HFMD include vesicles of hand, foot and mouth in young children and severe inflammatory CNS lesions. In this study, experimentally CA16 infected tree shrews(Tupaia belangeri) were used to investigate CA16 pathogenesis. The results showed that both the body temperature and the percentages of blood neutrophilic granulocytes / monocytes of CA16 infected tree shrews increased at 4-7 days post infection. Dynamic distributions of CA16 in different tissues and stools were found at different infection stages. Moreover, the pathological changes in CNS and other organs were also observed. These findings indicate that tree shrews can be used as a viable animal model to study CA16 infection.展开更多
手足口病(hand-foot and mouth disease,HFMD)是一种全球性的传染病,其主要病原体为肠道病毒71型(enterovirus 71,EV71)和柯萨奇病毒A组16型(cosackievirus A 16,CA16)。EV71与CA16同属于小核糖核酸(ribonucleic acid,RNA)病毒科(Picorn...手足口病(hand-foot and mouth disease,HFMD)是一种全球性的传染病,其主要病原体为肠道病毒71型(enterovirus 71,EV71)和柯萨奇病毒A组16型(cosackievirus A 16,CA16)。EV71与CA16同属于小核糖核酸(ribonucleic acid,RNA)病毒科(Picornaviridae)肠道病毒属(Enterovirus)肠道病毒A型(Human enterovirus A)的成员,两者在病原学、感染和复制特征以及感染导致的临床症状和免疫反应有相似的方面,但也有明显不同的方面。现将对EV71和CA16相关的病原学、感染和复制特征以及临床症状和免疫反应研究进行综述。展开更多
目的了解吉林省2012年手足口病（Hand,Foot and Mouth Disease;HFMD）来源的柯萨奇病毒A组16型（Coxsackie Virus Group A Type 16,CVA16）基因亲缘关系及氨基酸位点的变异情况。方法收集吉林省2012年73例HFMD临床标本,病毒分离后对其...目的了解吉林省2012年手足口病（Hand,Foot and Mouth Disease;HFMD）来源的柯萨奇病毒A组16型（Coxsackie Virus Group A Type 16,CVA16）基因亲缘关系及氨基酸位点的变异情况。方法收集吉林省2012年73例HFMD临床标本,病毒分离后对其中22株的VP1编码区部分核苷酸进行序列测定和分析。结果 22株CVA16中,12株为B1a基因亚型,10株为B1b基因亚型,12株B1a基因亚型氨基酸同源性为95.0%-97.8%,10株B1b基因亚型氨基酸同源性为94.3%-97.0%;有8株在第23位氨基酸位点发生了亮氨酸（Leucine,L）→蛋氨酸（Methionine,M）的变异,其余14株在该氨基酸位点均为L。结论 CVA16为吉林省HFMD的主要病原体之一,毒株间的同源性较高,均属于B基因群,在系统进化树上分别属于两个进化分支。展开更多
目的分析2017年平顶山市手足口病的病原学特征,为平顶山市手足口病的预防控制工作提供病原学依据。方法选取2017年平顶山市手足口病病例作为研究对象,采集手足口病患者的粪便或肛拭子标本,进行总肠道病毒、柯萨奇病毒A组16型(Coxsackie ...目的分析2017年平顶山市手足口病的病原学特征,为平顶山市手足口病的预防控制工作提供病原学依据。方法选取2017年平顶山市手足口病病例作为研究对象,采集手足口病患者的粪便或肛拭子标本,进行总肠道病毒、柯萨奇病毒A组16型(Coxsackie virus group A type 16, Cox Al6)和肠道病毒71型(Enterovirus type 71, EV71)病原学检测,并利用描述流行病学方法对检测结果进行分析。结果在所采集的320例手足口病病例标本中,肠道病毒阳性226份,总检出率为70.63%;其中Cox Al6、EV 71、EV 71+Cox Al6和其它肠道病毒等4种(类)病毒的检出数分别为42份、82份、1份和101份,分别占肠道病毒阳性总数的18.58%(42/226)、36.28%(82/226)、0.44%(1/226)和44.69%(101/226)。患者以3岁以下儿童为主,男性多于女性,以散居儿童为主。结论应持续加强当地儿童手足口病的病原学监测,并建议进行其他肠道病毒的分型检测,动态掌握手足口病病原学变化规律,为手足口病防治提供明确的病原学依据。展开更多
基金supported by the National High-Tech R&D Program(2014ZX09102042)the National Natural Science Foundation of China(81373142)the Natural Science Foundation of Yunnan Province(2012ZA009)
文摘Coxsackie virus A16(CA16) is commonly recognized as one of the main human pathogens of hand-foot-mouth disease(HFMD). The clinical manifestations of HFMD include vesicles of hand, foot and mouth in young children and severe inflammatory CNS lesions. In this study, experimentally CA16 infected tree shrews(Tupaia belangeri) were used to investigate CA16 pathogenesis. The results showed that both the body temperature and the percentages of blood neutrophilic granulocytes / monocytes of CA16 infected tree shrews increased at 4-7 days post infection. Dynamic distributions of CA16 in different tissues and stools were found at different infection stages. Moreover, the pathological changes in CNS and other organs were also observed. These findings indicate that tree shrews can be used as a viable animal model to study CA16 infection.
文摘手足口病(hand-foot and mouth disease,HFMD)是一种全球性的传染病,其主要病原体为肠道病毒71型(enterovirus 71,EV71)和柯萨奇病毒A组16型(cosackievirus A 16,CA16)。EV71与CA16同属于小核糖核酸(ribonucleic acid,RNA)病毒科(Picornaviridae)肠道病毒属(Enterovirus)肠道病毒A型(Human enterovirus A)的成员,两者在病原学、感染和复制特征以及感染导致的临床症状和免疫反应有相似的方面,但也有明显不同的方面。现将对EV71和CA16相关的病原学、感染和复制特征以及临床症状和免疫反应研究进行综述。
文摘目的了解吉林省2012年手足口病（Hand,Foot and Mouth Disease;HFMD）来源的柯萨奇病毒A组16型（Coxsackie Virus Group A Type 16,CVA16）基因亲缘关系及氨基酸位点的变异情况。方法收集吉林省2012年73例HFMD临床标本,病毒分离后对其中22株的VP1编码区部分核苷酸进行序列测定和分析。结果 22株CVA16中,12株为B1a基因亚型,10株为B1b基因亚型,12株B1a基因亚型氨基酸同源性为95.0%-97.8%,10株B1b基因亚型氨基酸同源性为94.3%-97.0%;有8株在第23位氨基酸位点发生了亮氨酸（Leucine,L）→蛋氨酸（Methionine,M）的变异,其余14株在该氨基酸位点均为L。结论 CVA16为吉林省HFMD的主要病原体之一,毒株间的同源性较高,均属于B基因群,在系统进化树上分别属于两个进化分支。
文摘目的分析2017年平顶山市手足口病的病原学特征,为平顶山市手足口病的预防控制工作提供病原学依据。方法选取2017年平顶山市手足口病病例作为研究对象,采集手足口病患者的粪便或肛拭子标本,进行总肠道病毒、柯萨奇病毒A组16型(Coxsackie virus group A type 16, Cox Al6)和肠道病毒71型(Enterovirus type 71, EV71)病原学检测,并利用描述流行病学方法对检测结果进行分析。结果在所采集的320例手足口病病例标本中,肠道病毒阳性226份,总检出率为70.63%;其中Cox Al6、EV 71、EV 71+Cox Al6和其它肠道病毒等4种(类)病毒的检出数分别为42份、82份、1份和101份,分别占肠道病毒阳性总数的18.58%(42/226)、36.28%(82/226)、0.44%(1/226)和44.69%(101/226)。患者以3岁以下儿童为主,男性多于女性,以散居儿童为主。结论应持续加强当地儿童手足口病的病原学监测,并建议进行其他肠道病毒的分型检测,动态掌握手足口病病原学变化规律,为手足口病防治提供明确的病原学依据。
