Arthrospira platensis is an economically important cyanobacterium; and it has been used widely in food and pharmaceutical industries. The phycocyanin(PC) from A. platensis is extremely valuable in medicine and molecul...Arthrospira platensis is an economically important cyanobacterium; and it has been used widely in food and pharmaceutical industries. The phycocyanin(PC) from A. platensis is extremely valuable in medicine and molecular biology due to its antioxidation and anti-tumoring activity and applicability as fluorescence protein tag. In present study, two recombinant plasmids, one contained the phycocyanobilin(PCB)-producing genes(hox1 and pcyA) while the other contained the phycobiliprotein gene(cpcB) and the lyase gene(either cpcS/U or cpcT), were constructed and synchronically transferred into E. coli in order to test the the activities of relevant lyases for catalysing PCB addition to CpcB during synthesizing fluorescent PC holo-β-subunit(β-PC) of A. platensis. As was evidenced by the fluorescence emitted at a peak specific for PC, CpcB was successfully synthesized in E. coli, to which co-expressed PCBs attached though at a relatively low efficiency. The results showed that the attachment of PCBs to CpcB were carried out mainly by co-expressed CpcS/U but CpcB also showed some autocatalytic activity. Currently, no CpcT activity was detected in this E. coli expression system. Further studies will be conducted to improve the efficiency of fluorescent PC synthesis in E. coli.展开更多
Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of eme...Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up.展开更多
Syncope is one kind of transient loss of consciousness,due to transient global cerebral hypoperfusion.It is sometimes misunderstood with other causes of transient loss of consciousness,like epilepsy,metabolic disorder...Syncope is one kind of transient loss of consciousness,due to transient global cerebral hypoperfusion.It is sometimes misunderstood with other causes of transient loss of consciousness,like epilepsy,metabolic disorders,head trauma,psychological factors,and so on.Syncope in children and adolescents is common and seriously impacts the quality of their lives.However,its pathogenesis,etiologies,diagnosis and management are complex.The studies on syncope attract great interest of pediatricians and great progress has been made in this interesting field.Based on the improvement achieved so far,Chinese Pediatric Cardiology Society(CPCS)successfully developed a guideline for the diagnosis and treatment of syncope in children and adolescents,which would greatly improve the level of clinical management of syncope.展开更多
On November 10th 2023,the State Council Information Office released the white paper titled "CPC(the Communist Party of China)Polices on the Governance of Xizang in the New Era:Approach and Achievements".The ...On November 10th 2023,the State Council Information Office released the white paper titled "CPC(the Communist Party of China)Polices on the Governance of Xizang in the New Era:Approach and Achievements".The white paper expounds that under the guidance of the CPC's governance strategy in the new era and with vigorous support from the people across the nation.展开更多
Nearly five decades after the third plenary session of the 11th Central Committee of the Communist Party of China(CPC)in 1978,which launched the historic reform and opening up policy that transformed China,the CPC hel...Nearly five decades after the third plenary session of the 11th Central Committee of the Communist Party of China(CPC)in 1978,which launched the historic reform and opening up policy that transformed China,the CPC held another third plenary session that promises to be potentially as consequential.The resolution passed on 18 July during the third plenary session of the 20th Central Committee of the CPC calls for comprehensively deepening reforms,thus paving the way for the country to march towards the final goal of a developed and prosperous country.展开更多
基金supported by the National Science and Technology Major Project of China (2008ZX08001-004)
文摘Arthrospira platensis is an economically important cyanobacterium; and it has been used widely in food and pharmaceutical industries. The phycocyanin(PC) from A. platensis is extremely valuable in medicine and molecular biology due to its antioxidation and anti-tumoring activity and applicability as fluorescence protein tag. In present study, two recombinant plasmids, one contained the phycocyanobilin(PCB)-producing genes(hox1 and pcyA) while the other contained the phycobiliprotein gene(cpcB) and the lyase gene(either cpcS/U or cpcT), were constructed and synchronically transferred into E. coli in order to test the the activities of relevant lyases for catalysing PCB addition to CpcB during synthesizing fluorescent PC holo-β-subunit(β-PC) of A. platensis. As was evidenced by the fluorescence emitted at a peak specific for PC, CpcB was successfully synthesized in E. coli, to which co-expressed PCBs attached though at a relatively low efficiency. The results showed that the attachment of PCBs to CpcB were carried out mainly by co-expressed CpcS/U but CpcB also showed some autocatalytic activity. Currently, no CpcT activity was detected in this E. coli expression system. Further studies will be conducted to improve the efficiency of fluorescent PC synthesis in E. coli.
文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up.
文摘Syncope is one kind of transient loss of consciousness,due to transient global cerebral hypoperfusion.It is sometimes misunderstood with other causes of transient loss of consciousness,like epilepsy,metabolic disorders,head trauma,psychological factors,and so on.Syncope in children and adolescents is common and seriously impacts the quality of their lives.However,its pathogenesis,etiologies,diagnosis and management are complex.The studies on syncope attract great interest of pediatricians and great progress has been made in this interesting field.Based on the improvement achieved so far,Chinese Pediatric Cardiology Society(CPCS)successfully developed a guideline for the diagnosis and treatment of syncope in children and adolescents,which would greatly improve the level of clinical management of syncope.
文摘On November 10th 2023,the State Council Information Office released the white paper titled "CPC(the Communist Party of China)Polices on the Governance of Xizang in the New Era:Approach and Achievements".The white paper expounds that under the guidance of the CPC's governance strategy in the new era and with vigorous support from the people across the nation.
文摘Nearly five decades after the third plenary session of the 11th Central Committee of the Communist Party of China(CPC)in 1978,which launched the historic reform and opening up policy that transformed China,the CPC held another third plenary session that promises to be potentially as consequential.The resolution passed on 18 July during the third plenary session of the 20th Central Committee of the CPC calls for comprehensively deepening reforms,thus paving the way for the country to march towards the final goal of a developed and prosperous country.
