The kudzu bug, Megacopta cribraria (Fabricius), recently became an invasive insect pest of soybean, Glycine max (L.) Merr. in the southeastern USA capable of reducing crop yield by as much as 60% if left untreated. To...The kudzu bug, Megacopta cribraria (Fabricius), recently became an invasive insect pest of soybean, Glycine max (L.) Merr. in the southeastern USA capable of reducing crop yield by as much as 60% if left untreated. To reduce these losses and minimize control costs, new methods for detecting kudzu bugs must be investigated in order to optimize control measures. One such method would be to detect volatiles released by kudzu bugs. An electronic portable device was developed to draw volatile organic compounds (VOCs) produced by kudzu bugs over carbon black-polymer composite sensors and measure the change in resistance for each sensor. Sensors made using polymers poly (bisphenol A carbonate) (p = 0.041), poly (styrene-co-allyl alcohol) (p = 0.017) and poly (vinylpyrrolidone) (p = 0.040) showed significant differences in resistance change for M. cribraria VOCs over the control. A logistic regression was 94.4% accurate based on the resistance changes from sensors made from polymers poly (4-vinylphenol), poly (styrene-co-allyl alcohol), and poly (vinylpyrrolidone) as features. These results indicate the capability of the device to detect kudzu bugs by detecting volatiles released by the insects.展开更多
[Objective] The morphology observation and investigation on occurrence and damage condition of Rhynhophorus ferrugineus were conducted.[Method] The samples of R.ferrugineus at different instars were collected from the...[Objective] The morphology observation and investigation on occurrence and damage condition of Rhynhophorus ferrugineus were conducted.[Method] The samples of R.ferrugineus at different instars were collected from the nurseries in Nanning City,and morphological observation was carried out under the entity microscope,and their characteristics were described.In Nanning City,with Palm plants as the investigation object,the random sampling investigation was conducted in damage peak period of R.ferrugineus,the total number of investigated plants and the number of damaged plants was recorded.[Result] The size,shape and arrangement of droplet-shaped spots in adult pronotum of different individuals of R.ferrugineus are inconsistent,the morphological characteristics of larvae in different host are the same,while the sizes of insect bodies of the same instar are different.The pests mainly distribute in part of nurseries and the concentrated cultivation places of palm trees in Nanning City,which mainly damage the palm plants such as R.rivularis,W.filifera,P.canariensis,B.nobilis and P.x loureirii,etc.within Nanning City.[Conclusion] The study laid the foundation for the research on the occurrence law and the control technique of R.ferrugineus in Nanning City.展开更多
筛豆龟蝽(Megacopta cribraria)是一种重要的大豆害虫,严重影响大豆的产量和品质。本文采用文献计量学的方法,以中国知网和Web of Science为数据源,整理出1958年至2023年发表的与筛豆龟蝽相关的中英文文献,利用VOSviewer等软件分析筛豆...筛豆龟蝽(Megacopta cribraria)是一种重要的大豆害虫,严重影响大豆的产量和品质。本文采用文献计量学的方法,以中国知网和Web of Science为数据源,整理出1958年至2023年发表的与筛豆龟蝽相关的中英文文献,利用VOSviewer等软件分析筛豆龟蝽的研究现状和发展趋势。结果表明,国内外共有146篇研究筛豆龟蝽的期刊文献,包括中文文献31篇,外文文献115篇,分布在134个出版物中。1958年中国学者发表了第一篇关于筛豆龟蝽的调查报告,国内外发文量多集中在2013年至2019年,美国和中国是发表论文数量排名前两位的国家。研究人员来自8个国家以及139个研究机构。国内热点关键词包括发生与防治、发生规律、大豆、环境适应性等,国外热点关键词包括龟蝽科、鉴定、多样性等。目前国内外关于筛豆龟蝽研究内容涉及昆虫学、昆虫生理生化、生物学、生态学等领域。展开更多
[Objective] The paper was to promote using synthetic sex pheromone lures to control tobacco cutworm moth（Spodoptera litura Fabricius） .[Method] Three layers of pheromone traps for S.litura were set in the field at t...[Objective] The paper was to promote using synthetic sex pheromone lures to control tobacco cutworm moth（Spodoptera litura Fabricius） .[Method] Three layers of pheromone traps for S.litura were set in the field at the density of 26 m×26 m（one trap/676 m2） to carry out field control test.[Result] The amount of egg massed reduced by 62.16% after trapping by sex pheromone under the density,and the larvae population was equivalent to those in the conventional control field with pesticides,the control effect was continuous,stable and significant.The larger the control area was,the better the effect was.[Conclusion] Using sex pheromone could effectively control S.litura and reduce pesticide usage for over 80% with economic,safe and efficient features,which should be incorporated into the technical control system of S.litura.展开更多
Megacopta cribraria (Hemiptera: Plataspidae) continually outbreaks due to suitable photoperiod in recent years. Effect of photoperiod on growth, development and reproduction of M. cribraria were assessed in this study...Megacopta cribraria (Hemiptera: Plataspidae) continually outbreaks due to suitable photoperiod in recent years. Effect of photoperiod on growth, development and reproduction of M. cribraria were assessed in this study. Results indicated that developmental duration, nutrient accumulation efficiency, and adult fecundity of M. cribraria were significantly different under 6 photoperiodical conditions. Developmental duration of nymph stage gradually tended to be shorter as day time increase. Body weights of 5th instar nymphs for 16 h and 4 h day time photoperiods were 5.2 mg and 4.6 mg, respectively. Moreover, longevity of adults tended to be longer as day time increase. However, for the short day photoperiod (4 L:20 D and 8 L:16 D), population showed no reproductive behaviors. Index of population trend increased with photoperiod extension and adults showed stronger reproductive capacity and longer longevity. This research identified the favorable photoperiodical conditions before outbreak. It may provided reference for ecological adaptability of M. cribraria, and contribute to the scientific basis for forecasting and controlling of M. cribraria.展开更多
We analyzed the composition and studied the bioactivity of secretion from the poplar sawfly, Stauronematus compressicornis （Fabricius）. A leaf-sandwich method was applied to test the bioactivity of the secretion. Co...We analyzed the composition and studied the bioactivity of secretion from the poplar sawfly, Stauronematus compressicornis （Fabricius）. A leaf-sandwich method was applied to test the bioactivity of the secretion. Coomassie brilliant blue, gas chromatography （GC） and other methods were used to analyze and carry out protein assays, amino acid analyses, fatty acid and cholesterol detection of the secretion. Results show that the secretion had a significant antifeeding effect on 4th instar larvae of the armyworm （Pseudaletia separata Walker）. Dissolved in 0.27 mol.L^-1 HCl, 0.1 mol.L^-1 NaOH and 0.05 mol.L^-1 Na2HPO4 separately, the secretions had antifeeding rates ranging from 22.7% to 49.6%. Meanwhile, the weights of the secretion-treated insects were significantly lower than those of the control. Biochemical detection showed that the proteins contained in the secretion were composed of 16 amino acids. The secretion also contained small amounts of cholesterol and fatty acids, which included lauric acid, myristic acid, myristoleic acid, seventeen carbonic acids and oleic acid.展开更多
The total protein increased in the gills and decreased in the muscle of the freshwater field crab Oziotelphusa senex senex at days 1 and 2 on exposure to lethal concentrations and at days 1 and 10 to sublethal concent...The total protein increased in the gills and decreased in the muscle of the freshwater field crab Oziotelphusa senex senex at days 1 and 2 on exposure to lethal concentrations and at days 1 and 10 to sublethal concentrations of furadan, endosulfan, chlorpyrifos, and a mixture of these three in a 100:10: 1 ratio. The increase in the gill protein was greater on exposure to the sublethal concentrations than to the lethal concentrations while the decrease in the muscle protein was greater on exposure to the lethal concentrations than to the sublethal concentrations. In the hepatopancreas, the protein content decreased on exposure to the lethal concentrations, but, in contrast, increased on exposure to the sublethal concentrations. These results clearly indicate that changes in the protein content are not only organ-dependent but also concentration-dependent, i.e., lethal versus sublethal. Irrespective of the changes in the total protein, the levels of free amino acids and the activities of protease, alanine and aspartate aminotransferases, and glutamate dehydrogenase increased in all the three organs of the crabs exposed to the lethal and sublethal concentrations, (more in lethal than in sublethal) and increased at a greater rate over time of exposure. Ammonia toxicity, measured by an increase in the hemolymph ammonia and a decrease in the urea, was also observed at the lethal concentrations of all the three pesticides. The ammonia and urea levels increased in the crabs exposed to the sublethal concentrations. Although the effect of each pesticide on the protein metabolism was similar, the degree of toxicity was the lowest on exposure to furadan, intermediate on exposure to endosulfan and chlorpyrifos, and cumulative on exposure to a mixture of the three pesticides展开更多
文摘The kudzu bug, Megacopta cribraria (Fabricius), recently became an invasive insect pest of soybean, Glycine max (L.) Merr. in the southeastern USA capable of reducing crop yield by as much as 60% if left untreated. To reduce these losses and minimize control costs, new methods for detecting kudzu bugs must be investigated in order to optimize control measures. One such method would be to detect volatiles released by kudzu bugs. An electronic portable device was developed to draw volatile organic compounds (VOCs) produced by kudzu bugs over carbon black-polymer composite sensors and measure the change in resistance for each sensor. Sensors made using polymers poly (bisphenol A carbonate) (p = 0.041), poly (styrene-co-allyl alcohol) (p = 0.017) and poly (vinylpyrrolidone) (p = 0.040) showed significant differences in resistance change for M. cribraria VOCs over the control. A logistic regression was 94.4% accurate based on the resistance changes from sensors made from polymers poly (4-vinylphenol), poly (styrene-co-allyl alcohol), and poly (vinylpyrrolidone) as features. These results indicate the capability of the device to detect kudzu bugs by detecting volatiles released by the insects.
基金Supported by Natural Science and Technology Fund Project in Guangxi Vocational Technical College of Agriculture(B070206)~~
文摘[Objective] The morphology observation and investigation on occurrence and damage condition of Rhynhophorus ferrugineus were conducted.[Method] The samples of R.ferrugineus at different instars were collected from the nurseries in Nanning City,and morphological observation was carried out under the entity microscope,and their characteristics were described.In Nanning City,with Palm plants as the investigation object,the random sampling investigation was conducted in damage peak period of R.ferrugineus,the total number of investigated plants and the number of damaged plants was recorded.[Result] The size,shape and arrangement of droplet-shaped spots in adult pronotum of different individuals of R.ferrugineus are inconsistent,the morphological characteristics of larvae in different host are the same,while the sizes of insect bodies of the same instar are different.The pests mainly distribute in part of nurseries and the concentrated cultivation places of palm trees in Nanning City,which mainly damage the palm plants such as R.rivularis,W.filifera,P.canariensis,B.nobilis and P.x loureirii,etc.within Nanning City.[Conclusion] The study laid the foundation for the research on the occurrence law and the control technique of R.ferrugineus in Nanning City.
文摘筛豆龟蝽(Megacopta cribraria)是一种重要的大豆害虫,严重影响大豆的产量和品质。本文采用文献计量学的方法,以中国知网和Web of Science为数据源,整理出1958年至2023年发表的与筛豆龟蝽相关的中英文文献,利用VOSviewer等软件分析筛豆龟蝽的研究现状和发展趋势。结果表明,国内外共有146篇研究筛豆龟蝽的期刊文献,包括中文文献31篇,外文文献115篇,分布在134个出版物中。1958年中国学者发表了第一篇关于筛豆龟蝽的调查报告,国内外发文量多集中在2013年至2019年,美国和中国是发表论文数量排名前两位的国家。研究人员来自8个国家以及139个研究机构。国内热点关键词包括发生与防治、发生规律、大豆、环境适应性等,国外热点关键词包括龟蝽科、鉴定、多样性等。目前国内外关于筛豆龟蝽研究内容涉及昆虫学、昆虫生理生化、生物学、生态学等领域。
基金Supported by Project in Yunnan Branch of China National Tobacco Company(2010YN77)~~
文摘[Objective] The paper was to promote using synthetic sex pheromone lures to control tobacco cutworm moth（Spodoptera litura Fabricius） .[Method] Three layers of pheromone traps for S.litura were set in the field at the density of 26 m×26 m（one trap/676 m2） to carry out field control test.[Result] The amount of egg massed reduced by 62.16% after trapping by sex pheromone under the density,and the larvae population was equivalent to those in the conventional control field with pesticides,the control effect was continuous,stable and significant.The larger the control area was,the better the effect was.[Conclusion] Using sex pheromone could effectively control S.litura and reduce pesticide usage for over 80% with economic,safe and efficient features,which should be incorporated into the technical control system of S.litura.
基金supported by the National Key R&D Program of China (No.2018YFD0201004)the China Agriculture Research System (No.CARS-04)the Open Research Fund of State Key Laboratory of Integrated Pest Management on Crops in Northeast China (No.DB201505KF03)
文摘Megacopta cribraria (Hemiptera: Plataspidae) continually outbreaks due to suitable photoperiod in recent years. Effect of photoperiod on growth, development and reproduction of M. cribraria were assessed in this study. Results indicated that developmental duration, nutrient accumulation efficiency, and adult fecundity of M. cribraria were significantly different under 6 photoperiodical conditions. Developmental duration of nymph stage gradually tended to be shorter as day time increase. Body weights of 5th instar nymphs for 16 h and 4 h day time photoperiods were 5.2 mg and 4.6 mg, respectively. Moreover, longevity of adults tended to be longer as day time increase. However, for the short day photoperiod (4 L:20 D and 8 L:16 D), population showed no reproductive behaviors. Index of population trend increased with photoperiod extension and adults showed stronger reproductive capacity and longer longevity. This research identified the favorable photoperiodical conditions before outbreak. It may provided reference for ecological adaptability of M. cribraria, and contribute to the scientific basis for forecasting and controlling of M. cribraria.
基金supported by the National Natural Science Foundation of China (Grant No. 30872032).
文摘We analyzed the composition and studied the bioactivity of secretion from the poplar sawfly, Stauronematus compressicornis （Fabricius）. A leaf-sandwich method was applied to test the bioactivity of the secretion. Coomassie brilliant blue, gas chromatography （GC） and other methods were used to analyze and carry out protein assays, amino acid analyses, fatty acid and cholesterol detection of the secretion. Results show that the secretion had a significant antifeeding effect on 4th instar larvae of the armyworm （Pseudaletia separata Walker）. Dissolved in 0.27 mol.L^-1 HCl, 0.1 mol.L^-1 NaOH and 0.05 mol.L^-1 Na2HPO4 separately, the secretions had antifeeding rates ranging from 22.7% to 49.6%. Meanwhile, the weights of the secretion-treated insects were significantly lower than those of the control. Biochemical detection showed that the proteins contained in the secretion were composed of 16 amino acids. The secretion also contained small amounts of cholesterol and fatty acids, which included lauric acid, myristic acid, myristoleic acid, seventeen carbonic acids and oleic acid.
文摘The total protein increased in the gills and decreased in the muscle of the freshwater field crab Oziotelphusa senex senex at days 1 and 2 on exposure to lethal concentrations and at days 1 and 10 to sublethal concentrations of furadan, endosulfan, chlorpyrifos, and a mixture of these three in a 100:10: 1 ratio. The increase in the gill protein was greater on exposure to the sublethal concentrations than to the lethal concentrations while the decrease in the muscle protein was greater on exposure to the lethal concentrations than to the sublethal concentrations. In the hepatopancreas, the protein content decreased on exposure to the lethal concentrations, but, in contrast, increased on exposure to the sublethal concentrations. These results clearly indicate that changes in the protein content are not only organ-dependent but also concentration-dependent, i.e., lethal versus sublethal. Irrespective of the changes in the total protein, the levels of free amino acids and the activities of protease, alanine and aspartate aminotransferases, and glutamate dehydrogenase increased in all the three organs of the crabs exposed to the lethal and sublethal concentrations, (more in lethal than in sublethal) and increased at a greater rate over time of exposure. Ammonia toxicity, measured by an increase in the hemolymph ammonia and a decrease in the urea, was also observed at the lethal concentrations of all the three pesticides. The ammonia and urea levels increased in the crabs exposed to the sublethal concentrations. Although the effect of each pesticide on the protein metabolism was similar, the degree of toxicity was the lowest on exposure to furadan, intermediate on exposure to endosulfan and chlorpyrifos, and cumulative on exposure to a mixture of the three pesticides
