Identification of the genetic locus associated with the crinkled leaf phenotype in a soybean(Glycine max L.)mutant by BSA-Seq technology

The leaf is the main photosynthetic organ of plants,and it plays a significant role in the yield of crop species.Identifying the causal mutations and candidate genes that underlie leaf phenotypic variation is an important breeding target in soybean grain yield improvement.An ethyl methyl sulfonate(EMS)-induced soybean mutant DWARFCRINKLEDLEAF1(DCL1)with an aberrant crinkled leaf phenotype was identified in the background of the soybean cultivar Zhongpin 661(Zp661).We constructed an F2 segregating population from a cross between Zp661 and DCL1 in order to investigate the genomic locus associated with the crinkled leaf trait.Using bulk segregant analysis(BSA)combined with the whole-genome resequencing method,the Euclidean distance(ED)correlation algorithm detected 12 candidate genomic regions with a total length of 20.32 Mb that were linked to the target trait.Following a comparative analysis of the sequence data for the wild-type and mutant pools,only one single nucleotide mutation(C:G>T:A)located on the first exon of Glyma.19G207100 was found to be associated with the trait.Candidate gene validation based on a CAPS marker derived from the detected single-nucleotide polymorphism(SNP)indicated a nucleotide polymorphism between the two parents.Therefore,our findings reveal that Glyma.19G207100,which is renamed as GLYCINE MAX DWARF CRINKLED LEAF 1(GmDCL1),is a promising candidate gene involved in the morphogenesis of the crinkled leaf trait of the soybean mutant DCL1.This study provides a basis for the functional validation of this gene,with prospects for soybean breeding targeting grain yield enhancement.

Control Techniques of Chinese Chestnut Yellow Crinkle

This paper initially delineates the symptoms,characteristics,and hazards associated with Chinese chestnut yellow crinkle,as well as the transmission and infection pathways of the disease.Additionally,it proposes the prevention and control strategies of the disease,including rigorous investigation and monitoring of the disease,enhanced monitoring of seedlings and scions,and integrated disease management techniques to promote the tree s health and vitality.These strategies serve to inform the prevention and control of Chinese chestnut yellow crinkle in production.

The Defense Effect of ABA against Turnip crinkle virus (TCV)

[ Objective ] The paper was to understand whether abscisic acid （ABA） participates the defense effect of hosts against Turnip crinkle virus （TCV）. [ Method] By external application of ABA and using the corresponding mutants, TCV coat protein levels of various plants were detected by western-blot, and the expression of defense-related genes was analyzed by RT-PCR. [Result] After external application of SA, JA, ET and ABA, only SA and ABA could significantly inhibit TCV accumulation. In arabidopsis-TCV interaction system, SA and ABA had antagonistic effect with each other, the defense effect against TCV induced by ABA was independent on SA pathway. [ Conclusion] The plants could activate distinct signal pathways to adjust the defense of hosts against virus.