Water Vapor Transport and Cross-Equatorial Flow over the Asian-Australia Monsoon Region Simulated by CMIP5 Climate Models

The summer mean water vapor transport （WVT） and cross-equatorial flow （CEF） over the Asian- Australian monsoon region simulated by 22 coupled atmospheric-oceanic general circulation models （AOGCMs） from the World Climate Research Programme＇s Coupled Model Intercomparison Project Phase 5 （CMIP5） were evaluated. Based on climatology of the twentieth-century simulations, most of models have a reason- ably realistic representation of summer monsoon WVT characterized by southeast water vapor conveyor belt over the South Indian Ocean and southwest belt from the Arabian Sea to the East Asian. The correlation coefficients between NCEP reanalysis and simulations of BCC-CSMI-1, BNU-ESM, CanESM2, FGOALS-s2, MIROC4h and MPI-ESM-LR are up to 0.8. The simulated CEF depicted by the meridional wind along the equator includes the Somali jet and eastern CEF in low atmosphere and the reverse circulation in upper atmosphere, which were generally consistent with NCEP reanalysis. Multi-model ensemble means （MME） can reproduce more reasonable climatological features in spatial distribution both of WVT and CEF. Ten models with more reasonable WVT simulations were selected for future projection studies, including BCC- CSMI-1, BNU-ESM, CanESM2, CCSM4, FGOALS-s2, FIO-ESM, GFDL-ESM2G, MRIOCS, MPI-ESM-LR and NorESM-1M. Analysis based on the future projection experiments in RCP （Representative Concentra- tion Pathway） 2.6, RCP4.5, RCP6 and RCP8.5 show that the global warming forced by different RCP scenarios will results in enhanced WVT over the Indian area and the west Pacific and weaken WVT in the low latitudes of tropical Indian Ocean.

The Role of Ocean Dynamics in the Cross-equatorial Energy Transport under a Thermal Forcing in the Southern Ocean

Under external heating forcing in the Southern Ocean,climate models project anomalous northward atmosphere heat transport(AHT)across the equator,accompanied by a southward shift of the intertropical convergence zone(ITCZ).Comparison between a fully coupled and a slab ocean model shows that the inclusion of active ocean dynamics tends to partition the cross-equatorial energy transport and significantly reduce the ITCZ shift response by a factor of 10,a finding which supports previous studies.To understand how ocean dynamics damps the ITCZ’s response to an imposed thermal heating in the Southern Ocean,we examine the ocean heat transport(OHT)and ocean circulation responses in a set of fully coupled experiments.Results show that both the Indo-Pacific and the Atlantic contribute to transport energy across the equator mainly through its Eulerian-mean component.However,different from previous studies that linked the changes in OHT to the changes in the wind-driven subtropical cells or the Atlantic meridional overturning circulation(AMOC),our results show that the cross-equatorial OHT anomaly is due to a broad clockwise overturning circulation anomaly below the subtropical cells(approximately bounded by the 5°C to 20°C isotherms and 50°S to 10°N).Further elimination of the wind-driven component,conducted by prescribing the climatological wind stress in the Southern Ocean heat perturbation experiments,leads to little change in OHT,suggesting that the OHT response is predominantly thermohaline-driven by air-sea thermal interactions.

Smart Cellulose‑Based Janus Fabrics with Switchable Liquid Transportation for Personal Moisture and Thermal Management

The Janus fabrics designed for personal moisture/thermal regulation have garnered significant attention for their potential to enhance human comfort.However,the development of smart and dynamic fabrics capable of managing personal moisture/thermal comfort in response to changing external environments remains a challenge.Herein,a smart cellulose-based Janus fabric was designed to dynamically manage personal moisture/heat.The cotton fabric was grafted with N-isopropylacrylamide to construct a temperature-stimulated transport channel.Subsequently,hydrophobic ethyl cellulose and hydrophilic cellulose nanofiber were sprayed on the bottom and top sides of the fabric to obtain wettability gradient.The fabric exhibits anti-gravity directional liquid transportation from hydrophobic side to hydrophilic side,and can dynamically and continuously control the transportation time in a wide range of 3–66 s as the temperature increases from 10 to 40℃.This smart fabric can quickly dissipate heat at high temperatures,while at low temperatures,it can slow down the heat dissipation rate and prevent the human from becoming too cold.In addition,the fabric has UV shielding and photodynamic antibacterial properties through depositing graphitic carbon nitride nanosheets on the hydrophilic side.This smart fabric offers an innovative approach to maximizing personal comfort in environments with significant temperature variations.

Increased excitatory amino acid transporter 2 levels in basolateral amygdala astrocytes mediate chronic stress–induced anxiety-like behavior

The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with emotions.Specifically, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal mice.After knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was obviously mitigated. Additionally, administration of an excitatory amino acid transporter 2 inhibitor in the basolateral amygdala yielded a notable reduction in anxiety level among mice subjected to stress. These results suggest that basolateral amygdala astrocytic excitatory amino acid transporter 2 plays a role in in the regulation of unpredictable chronic mild stress-induced anxiety-like behavior by impacting the activity of local glutamatergic neurons, and targeting excitatory amino acid transporter 2 in the basolateral amygdala holds therapeutic promise for addressing anxiety disorders.

The sexually dimorphic expression of glutamate transporters and their implication in pain after spinal cord injury

In addition to the loss of motor function,~60% of patients develop pain after spinal cord injury.The cellular-molecular mechanisms are not well understood,but the data suggests that plasticity within the rostral,epicenter,and caudal penumbra of the injury site initiates a cellularmolecular interplay that acts as a rewiring mechanism leading to central neuropathic pain.Sprouting can lead to the formation of new connections triggering abnormal sensory transmission.The excitatory glutamate transporters are responsible for the reuptake of extracellular glutamate which makes them a critical target to prevent neuronal hyperexcitability and excitotoxicity.Our previous studies showed a sexually dimorphic therapeutic window for spinal cord injury after treatment with the selective estrogen receptor modulator tamoxifen.In this study,we investigated the anti-allodynic effects of tamoxifen in male and female rats with spinal cord injury.We hypothesized that tamoxifen exerts anti-allodynic effects by increasing the expression of glutamate transporters,leading to reduced hyperexcitability of the secondary neuron or by decreasing aberrant sprouting.Male and female rats received a moderate contusion to the thoracic spinal cord followed by subcutaneous slow-release treatment of tamoxifen or matrix pellets as a control(placebo).We used von Frey monofilaments and the“up-down method”to evaluate mechanical allodynia.Tamoxifen treatment decreased allodynia only in female rats with spinal cord injury revealing a sexdependent effect.The expression profile of glutamatergic transporters(excitatory amino acid transporter 1/glutamate aspartate transporter and excitatory amino acid transporter 2/glutamate transporter-1)revealed a sexual dimorphism in the rostral,epicenter,and caudal areas of the spinal cord with a pattern of expression primarily on astrocytes.Female rodents showed a significantly higher level of excitatory amino acid transporter-1 expression while male rodents showed increased excitatory amino acid transporter-2 expression compared with female rodents.Analyses of peptidergic(calcitonin gene-related peptide-α)and non-peptidergic(isolectin B4)fibers outgrowth in the dorsal horn after spinal cord injury showed an increased calcitonin gene-related peptide-α/isolectin B4 ratio in comparison with sham,suggesting increased receptive fields in the dorsal horn.Although the behavioral assay shows decreased allodynia in tamoxifen-treated female rats,this was not associated with overexpression of glutamate transporters or alterations in the dorsal horn laminae fibers at 28 days post-injury.Our findings provide new evidence of the sexually dimorphic expression of glutamate transporters in the spinal cord.The dimorphic expression revealed in this study provides a therapeutic opportunity for treating chronic pain,an area with a critical need for treatment.

Variations of the Summer Somali and Australia Cross-Equatorial Flows and the Implications for the Asian Summer Monsoon

The temporal variations during 1948-2010 and vertical structures of the summer Somali and Australia cross-equatorial flows （CEFs） and the implications for the Asian summer monsoon were explored in this study. The strongest southerly and northerly CEFs exist at 925 hPa and 150 hPa level, respectively. The low-level Somali （LLS） CEFs were significantly connected with the rainfall in most regions of India （especially the monsoon regions）, except in a small area in southwest India. In comparison to the climatology, the low- level Australia （LLA） CEFs exhibited stronger variations at interannual time scale and are more closely connected to the East Asian summer monsoon circulation than to the LLS CEFs. The East Asian summer monsoon circulation anomalies related to stronger LLA CEFs were associ- ated with less water vapor content and less rainfall in the region between the middle Yellow River and Yangtze River and with more water vapor and more rainfall in southern China. The sea-surface tempera- ture anomalies east of Australia related to summer LLA CEFs emerge in spring and persist into summer, with implications for the seasonal prediction of summer rainfall in East Asia. The connection between the LLA CEFs and East Asian summer monsoon rainfall may be partly due to its linkage with E1 Nino-Southern Oscillation. In addition, both the LLA and LLS CEFs .exhibited interdecadal shifts in the late 1970s and the late 1990s, consistent with the phase shifts of Pacific Decadal Oscillation （PDO）.

The Genesis of Tropical Cyclone Bilis(2000) Associated with Cross-equatorial Surges

The purpose of this paper is to explore how a tropical cyclone forms from a pre-existing large-scale depression which has been observed and associated with cross-equatorial surges in the western North Pacific. Tropical cyclone Bilis(2000) was selected as the case to study.The research data used are from the results of the non-hydrostatic mesoscale model(MM5),which has successfully simulated the transformation of a pre-existing weak large-scale tropical depression into a strong tropical storm.The scale separation technique is used to separate the synoptic-scale and sub-synoptic-scale fields from the model output fields. The scale-separated fields show that the pre-existing synoptic-scale tropical depression and the subsynoptic scale tropical cyclone formed later were different scale systems from beginning to end.It is also shown that the pre-existing synoptic-scale tropical depression did not contract to become the tropical cyclone. A series of weak,sub-synoptic-scale low and high pressure systems appeared and disappeared in the synopticscale depression,with one of the low systems near the center of the synoptic-scale depression having deepened to become the tropical cyclone. The roles of the synoptic-scale flow and the sub-synoptic scale disturbances in the formation of the tropical cyclone are investigated by diagnoses of the scale-separated vertical vorticity equation.The results show that the early development of the sub-synoptic scale vortex was fundamentally dependent on the strengthening synoptic-scale environmental depression.The depression was strengthened by cross-equatorial surges,which increased the convergence of the synoptic-scale depression at low levels and triggered the formation of the tropical cyclone.

CLIMATOLOGICAL VARIATION OF GLOBAL CROSS-EQUATORIAL FLOWS FOR THE PERIOD 1948-2004

By using monthly NCEP/NCAR meridional gridpoint wind data at the levels of 1000, 850, 700, 600, 500, 400, 300, 200, 150 and 100 hPa from 1948 to 2004, the intensity of global cross-equatorial flows is calculated. The spatial and temporal variation of global cross-equatorial flows at the 850-hPa level are shown and discussed. The results show that the strength of the 850-hPa global cross-equatorial flows represent obvious long-term variation and interdecadal change during the period. Evidence suggests that the cross-equatorial flow of the passages at 45 - 50 °E in June to August, 105 - 115 °E in May to September, 130 - 140 °E in May to September and May to November and 20 - 25 °E in February to April intensified and that the cross-equatorial flow of the passages at 50 - 35 °W in June to August weaken in the past 57 years, with an increase of 0.25m/s/10a for summer Somali Jet and increase of 0.32 m/s/10a for cross- equatorial flow at 130 - 140 °E in May to September The results of Singular Spectrum Analysis （SSA） for the time series indicate that for the cross-equatorial flow at 850 hPa, the interdecadal and long-term trend changes are 35% - 45%, and the interarmual variation is no more than 30%, in variance contribution. The results also reveal that the interarmual variation of intensity of the summer cross-equatorial flows in the Pacific is significantly correlated with Southern Oscillation. With weak Southern Oscillation, strong crossequatorial flows in Pacific will happen, though the summer Somali Jet is only a little positively correlated with North Atlantic Oscillation （NAO）.

NUMERICAL SIMULATION OF SSTA IMPACTS UPON THE INTERDECADAL VARIATION OF THE CROSS-EQUATORIAL FLOWS IN EASTERN HEMISPHERE

Impacts of regional sea surface temperature(SST)anomalies on the interdecadal variation of the cross-equatorial flows(CEFs)in Eastern Hemisphere are studied using numerical simulations with a global atmospheric circulation model(NCAR CAM3)driven with 1950-2000 monthly SSTs in different marine areas(the globe,extratropics,tropics,tropical Indian Ocean-Pacific,and tropical Pacific)and ERA-40reanalysis data.Results show that all simulations,except the one driven with extratropical SSTs,can simulate the interdecadal strengthening of CEFs around Somali,120oE,and 150oE that occurred in the midand late-1970s.Among those simulated CEFs,the interdecadal variability in Somali and its interdecadal relationship with the East Asian summer monsoon are in better agreement with the observations,suggesting that changes in the SSTs of tropical oceans,especially the tropical Pacific,play a crucial role in the interdecadal variability of CEFs in Somali.The interdecadal change of CEFs in Somali is highly associated with the interdecadal variation of tropical Pacific SST.As the interdecadal warmer(colder)SST happens in the tropical Pacific,a"sandwich"pattern of SST anomalies,i.e."+,-,+"("-,+,-"),will occur in the eastern tropical Pacific from north to south with a pair of anomalous anticyclone(cyclone)at the lower troposphere;the pair links to another pair of anomalous cyclone(anticyclone)in the tropical Indian Ocean through an atmospheric bridge,and thus strengthens(weakens)the CEFs in Somali.

Vertical Structure of Interannual Variability in Cross-Equatorial Flows over the Maritime Continent and Indian Ocean in Boreal Summer

This study investigates the vertical structure of variability in the cross-equatorial flows(CEFs)over the Maritime Continent and Indian Ocean in boreal summer,based on three reanalysis datasets:ERA-Interim,JRA-55 and NCEP-2.The results show a high consistency in the interannual variability among the reanalysis datasets,especially between ERAInterim and JRA-55,while great uncertainty exists in the decadal or long-term changes of CEFs.Further analyses on the interannual variability in CEFs indicate that there is a significant negative relationship between the upper-and lower-level CEFs over the Maritime Continent—that is,the northerlies at the upper level and southerlies at the lower level are both enhanced or weakened.This seesaw pattern is also significantly related to the CEFs over the Indian Ocean at the upper level and lower level(i.e.,the Somali jet).This close relationship between the upper-and lower-level CEFs and between the Maritime Continent and Indian Ocean is manifested as the leading mode of equatorial meridional winds in the vertical-zonal section over the Maritime Continent and Indian Ocean.Finally,it is found that ENSO is closely related to the vertical leading mode,and to all the CEFs at the upper and lower levels over the Maritime Continent and Indian Ocean.

EFFECTS OF SLOPING BOTTOM ON THE DEEPCROSS-EQUATORIAL BOUNDARY CURRENT

This paper reports study focusing on the effects of sloping bottom on the deep cross-equatorial boundary current, and discusses model and laboratory experiment results showing that the southward that the southward intrusion distance and flow speed of the western boundary current depend on the bottom slope variation rate,the difference between and and are the current thickness at eastward edge and westward edge, respectively), and the net mass transport.

Modulation by the Atlantic Multidecadal Oscillation of the intensity of the interannual seesaw between the Somali and Australian cross-equatorial flows

The decadal variation of the intensity of the interannual opposite connection （seesaw） between the Somali and Australian cross-equatorial flows （CEFs） is investigated. During the period prior to the mid-1960s, and after the early-2000s, the intensity of the interannual seesaw connection is significantly weaker relative to the period between. Such interdecadal shifts in the interannual seesaw intensity bear a resemblance to the decadal shift in ENSO＇s strength, and can be further attributed to the phase transition of the Atlantic Multidecadal Oscillation （AMO）. When the AMO is in a positive phase, the ENSO amplitude reduces and the seesaw strength becomes weakened, and vice versa.The historical simulation outputs of the CMIP5 models are used to verify the connection, and a similar result was obtained. Thus, the notion that the intensity of the interannual opposite connection （seesaw） between the CEFs is modulated by the AMO is robust.

VARIATION FEATURES OF SOMALI CROSS-EQUATORIAL FLOW AND ITS IMPACT ON THE LOCATION OF THE SUBTROPICAL HIGH RIDGE FROM JULY TO SEPTEMBER

The variation features of the cross-equatorial flow and its impact on the ridge position of the subtropical high have been analyzed in this paper. It is shown as follows. (1) The intensity of the Somali cross-equatorial flow is increasing in winter and summer in the past 44 years and the airflow of Northern Hemisphere exchanges more and more intensively with that of Southern Hemisphere. (2) The Somali cross-equatorial flow in May has the most impact on the ridge position of the subtropical high in the typhoon season, presenting a positive correlation. (3) The diagnosis is consistent with the real situation in 2005.

INTERANNUAL VARIATION CHARACTERISTICS OF EAST HEMISPHERIC CROSS-EQUATORIAL FLOW AND ITS CONCURRENT RELATIONSHIPS WITH TEMPERATURE AND RAINFALL IN CHINA

The NCEP/NCAR global reanalysis data were used to analyze the interannual variation characteristics of the cross-equatorial flow (CEF) and its concurrent relationships with temperature and rainfall in China. The results indicated that CEF changes more in summer than in winter. As the main flow channel in summer, the Somali CEF changes in a way that does not markedly influence the changes in the CEF total except for winter. The summer CEF total has two sudden increases and one sudden decrease during the last century while the winter total has just one decrease. Long-period data show that the correlation between CEF and summer rainfall in China is not very significant, but is different before and after the 1970s, which is due to CEF's close links with the East Asia summer monsoon. Winter CEF's correlation with concurrent winter temperature in northern and southern China varies with the relationship between CEF and sea-level pressure in different areas.

Relationship between cross-equatorial flows over the Bay of Bengal and Australia in boreal summer:Role of tropical diabatic heating

The interannual variability of cross-equatorial flows(CEFs)over the Asian–Australian monsoon(AAM)region during boreal summer was analyzed by applying the empirical orthogonal function(EOF)method to the meridional wind at 925 h Pa.The first mode(EOF1)exhibits an in-phase relationship among different CEF channels over the AAM region,which has received much attention owing to its tight linkage with ENSO.By contrast,the second mode(EOF2)possesses an out-of-phase relationship between the Bay of Bengal(BOB)CEF(90°E)and Australian CEF,among which the New Guinea CEF near 150°E shows the most significant opposite correlation with the BOB CEF.Observational and numerical model results suggest that the equatorially asymmetric heat source(sink)over the western(eastern)Maritime Continent,closely associated with the in-situ sea surface temperature anomaly,can induce cross-equatorial northerly(southerly)flow into the heating hemisphere,which dominates the out-of-phase relationship between the BOB and New Guinea CEFs.Furthermore,an equatorially symmetric heating over the central Pacific may indirectly change the CEFs by modulating the zonal atmospheric circulation near the Maritime Continent.

Investigating the elliptic anisotropy of identified particles in p-Pb collisions with a multi-phase transport model

The elliptic azimuthal anisotropy coefficient(v_(2))of the identified particles at midrapidity(|η|<0.8)was investigated in p-Pb collisions at√s_(NN)=5.02 TeV using a multi-phase transport model(AMPT).The calculations of differential v_(2)based on the advanced flow extraction method of light flavor hadrons(pions,kaons,protons,andΛ)in small collision systems were extended to a wider transverse momentum(p_(T))range of up to 8 GeV/c for the first time.The string-melting version of the AMPT model provides a good description of the measured p_(T)-differential v_(2)of the mesons but exhibits a slight deviation from the baryon v_(2).In addition,we observed the features of mass ordering at low p_(T)and the approximate number-of-constituentquark(NCQ)scaling at intermediate p_(T).Moreover,we demonstrate that hadronic rescattering does not have a significant impact on v_(2)in p-Pb collisions for different centrality selections,whereas partonic scattering dominates in generating the elliptic anisotropy of the final particles.This study provides further insight into the origin of collective-like behavior in small collision systems and has referential value for future measurements of azimuthal anisotropy.

Host-induced gene silencing of the Verticillium dahliae thiamine transporter protein gene(VdThit)confers resistance to Verticillium wilt in cotton

Verticillium wilt(VW),induced by the soil-borne fungus Verticillium dahliae(Vd),poses a substantial threat to a diverse array of plant species.Employing molecular breeding technology for the development of cotton varieties with heightened resistance to VW stands out as one of the most efficacious protective measures.In this study,we successfully generated two stable transgenic lines of cotton(Gossypium hirsutum L.),VdThitRNAi-1 and VdThit-RNAi-2,using host-induced gene silencing(HIGS)technology to introduce double-stranded RNA(dsRNA)targeting the thiamine transporter protein gene(VdThit).Southern blot analysis confirmed the presence of a single-copy insertion in each line.Microscopic examination showed marked reductions in the colonization and spread of Vd-mCherry in the roots of VdThit-RNAi cotton compared to wild type(WT).The corresponding disease index and fungal biomass of VdThit-RNAi-1/2 also exhibited significant reductions.Real-time quantitative PCR(qRT-PCR)analysis demonstrated a substantial inhibition of VdThit expression following prolonged inoculation of VdThit-RNAi cotton.Small RNA sequencing(sRNA-Seq)analysis revealed the generation of a substantial number of VdThit-specific siRNAs in the VdThit-RNAi transgenic lines.Additionally,the silencing of VdThit by the siVdThit produced by VdThit-RNAi-1/2 resulted in the elevated expression of multiple genes involved in the thiamine biosynthesis pathway in Vd.Under field conditions,VdThit-RNAi transgenic cotton exhibited significantly enhanced disease resistance and yield compared with WT.In summary,our findings underscore the efficacy of HIGS targeting VdThit in restraining the infection and spread of Vd in cotton,thereby potentially enabling the development of cotton breeding as a promising strategy for managing VW.

Identification of the lysine and histidine transporter family in Camellia sinensis and the characterizations in nitrogen utilization

In plants,the lysine and histidine transporter(LHT)family represent a class of proteins that mediate the uptake,translocation,and utilization of amino acids.The tea plant(Camellia sinensis)is a perennial evergreen with a relatively high level of amino acids.However,systematic identification and molecular characterization of the LHT gene family has rarely been reported in tea plants.In this study,22 CsLHTs were identified from the‘Shuchazao’genome and classified into two groups.The modeled three-dimensional structure and the conserved domains presented a high similarity among the LHTs proteins.Moreover,it was predicted that a few genes were conserved through the analysis of the physiochemical characters,structures and cis-elements in promoters.The expression patterns in tea plants revealed that CsLHT7 was mainly expressed in the roots,and CsLHT4 and CsLHT11 exhibited relatively high expression in both the roots and leaves.Moreover,the expression of all three genes could be induced by organic nitrogen.Additionally,heterogeneous expression of CsLHT4,CsLHT7 and CsLHT11 in Arabidopsis thaliana decreased the aerial parts biomass compared with that in WT plants while significantly increased the rosette biomass only for CsLHT11transgenic plants versus WT plants.Overall,our results provide fundamental information about CsLHTs and potential genes in N utilization for further analysis in tea plants.

Hydromechanical characterization of gas transport amidst uncertainty for underground nuclear explosion detection

Given the challenge of definitively discriminating between chemical and nuclear explosions using seismic methods alone,surface detection of signature noble gas radioisotopes is considered a positive identification of underground nuclear explosions(UNEs).However,the migration of signature radionuclide gases between the nuclear cavity and surface is not well understood because complex processes are involved,including the generation of complex fracture networks,reactivation of natural fractures and faults,and thermo-hydro-mechanical-chemical(THMC)coupling of radionuclide gas transport in the subsurface.In this study,we provide an experimental investigation of hydro-mechanical(HM)coupling among gas flow,stress states,rock deformation,and rock damage using a unique multi-physics triaxial direct shear rock testing system.The testing system also features redundant gas pressure and flow rate measurements,well suited for parameter uncertainty quantification.Using porous tuff and tight granite samples that are relevant to historic UNE tests,we measured the Biot effective stress coefficient,rock matrix gas permeability,and fracture gas permeability at a range of pore pressure and stress conditions.The Biot effective stress coefficient varies from 0.69 to 1 for the tuff,whose porosity averages 35.3%±0.7%,while this coefficient varies from 0.51 to 0.78 for the tight granite(porosity<1%,perhaps an underestimate).Matrix gas permeability is strongly correlated to effective stress for the granite,but not for the porous tuff.Our experiments reveal the following key engineering implications on transport of radionuclide gases post a UNE event:(1)The porous tuff shows apparent fracture dilation or compression upon stress changes,which does not necessarily change the gas permeability;(2)The granite fracture permeability shows strong stress sensitivity and is positively related to shear displacement;and(3)Hydromechanical coupling among stress states,rock damage,and gas flow appears to be stronger in tight granite than in porous tuff.

Global characterization of OsPIP aquaporins reveals that the H_(2)O_(2)transporter OsPIP2;6 increases resistance to rice blast

Plasma membrane intrinsic proteins(PIPs)are conserved plant aquaporins that transport small molecules across the plasma membrane to trigger instant stress responses and maintain cellular homeostasis under biotic and abiotic stress.To elucidate their roles in plant immunity to pathogen attack,we characterized the expression patterns,subcellular localizations,and H_(2)O_(2)-transport ability of 11 OsPIPs in rice(Oryza sativa),and identified OsPIP2;6 as necessary for rice disease resistance.OsPIP2;6 resides on the plasma membrane and facilitates cytoplasmic import of the immune signaling molecule H_(2)O_(2).Knockout of OsPIP2;6 increases rice susceptibility to Magnaporthe oryzae,indicating a positive function in plant immunity.OsPIP2;6 interacts with OsPIP2;2,which has been reported to increase rice resistance to pathogens via H_(2)O_(2)transport.Our findings suggest that OsPIP2;6 cooperates with OsPIP2;2 as a defense signal transporter complex during plant–pathogen interaction.