The actively heated fiber-optic(AHFO)technology has become emerged as a research focus due to its advantages of distributed,real-time measurement and good durability.These attributes have led to the gradual applicatio...The actively heated fiber-optic(AHFO)technology has become emerged as a research focus due to its advantages of distributed,real-time measurement and good durability.These attributes have led to the gradual application of AHFO technology to the water content measurement of in situ soil.However,all existing in situ applications of AHFO technology fail to consider the effect of soilesensor contact quality on water content measurements,limiting potential for the wider application of AHFO technology.To address this issue,the authors propose a method for determining the soilesensor thermal contact resistance based on the principle of an infinite cylindrical heat source.This is then used to establish an AHFO water content measurement technology that considers the thermal contact resistance.The reliability and validity of the new measurement technology are explored through a laboratory test and a field case study,and the spatial-temporal evolution of the soil water content in the case is revealed.The results demonstrate that method for determining the soilesensor thermal contact resistance is highly effective and applicable to all types of soils.This method requires only the moisture content,dry density,and thermal response of the in situ soil to be obtained.In the field case,the measurement error of soil water content between the AHFO method,which takes into account the thermal contact resistance,and the neutron scattering method is only 0.011.The water content of in situ soil exhibits a seasonal variation,with an increase in spring and autumn and a decrease in summer and winter.Furthermore,the response of shallow soils to precipitation and evaporation is significant.These findings contribute to the enhancement of the accuracy of the AHFO technology in the measurement of the water content of in situ soils,thereby facilitating the dissemination and utilization of this technology.展开更多
Based on the features and requirements of gas drainage system, an optimized explosion resistance technology is done after a comprehensive analysis and research about the triple IR (Infrared Ray) flame detection tech...Based on the features and requirements of gas drainage system, an optimized explosion resistance technology is done after a comprehensive analysis and research about the triple IR (Infrared Ray) flame detection technology, explosion resistance valve technology and explosion resistance control technology. An intelligent PLC (Programmable Logic Controller) resistance control system is designed which can cut offthe gas branch quickly and accurately, and the controller have automatic pressure maintaining function, valve rotation limit function, remote and local control interlock function. The reliability and rationality of explosion resistance technology is verified by gas pipeline explosion propagation and resistance simulation test. Overall response time of explosion resistance system is less than 100ms, and the spread of fire in gas pipeline can be prevented effectively.展开更多
Founded in 2012,the National Joint Engineering Research Center of High Performance Metal Wear Resistant Materials Technology at Jinan University,one of the'211'key national universities in China,specializes in...Founded in 2012,the National Joint Engineering Research Center of High Performance Metal Wear Resistant Materials Technology at Jinan University,one of the'211'key national universities in China,specializes in the research and development of iron based wear resistant materials and their casting technologies to provide support to the production process.The Research Center serves the'Guangdong Province Ceeusro Innovation Platform for Common Technology of High Performance Wear Resistant Materials”,“Guangdong Province Engineering Research Center for Wear Resistant and Special Functional Materials”.展开更多
One of the major challenges in designing and fabricating Spintronic devices is the choice of both, Materials and the Technology, along with understanding the intricacies of the Designing aspects. In this communication...One of the major challenges in designing and fabricating Spintronic devices is the choice of both, Materials and the Technology, along with understanding the intricacies of the Designing aspects. In this communication, we have attempted to briefly discuss these factors, with an aim to draw the attention of the Materials Scientists and Technologists to this serious challenge, in the direction of which, though a lot of research and development work has been done, still needs more concerted efforts to be made in order to make the Spintronic devices that can offer good efficiency for maximizing their usefulness.展开更多
TiN/Ti multi-permeating alloying layer has been formed on the low carbon steel by means of the double glow-discharge plasma surface alloying technique and hollow-cathode effect. The alloying layer was detected by axio...TiN/Ti multi-permeating alloying layer has been formed on the low carbon steel by means of the double glow-discharge plasma surface alloying technique and hollow-cathode effect. The alloying layer was detected by axiovert 25 CA optical microscope with computer analyzing software (LEC), GDA-2 glow discharge spectroscopy (GDS), X-ray diffraction (XRD) and galvanochemical method. The results showed that the thickness of TiN/Ti multi-permeating alloying layer was about 10μm, the content of Ti on the surface was up to 63.48 wt% and the content of N was up to 12.46 wt%. The atom Ti and N concentrations changed gradually across the depth of the alloying layer and the preferred orientation of TiN/Ti alloying layer was crystal surface (200). The multi-permeating alloying layer and substrate were combined through metallurgy. The surface appearances of the multi-permeating alloying layer were uniform and of a compact cellular structure. The hardness of the surface was about 1600-3000 HV0.1. The corrosion resistance of the permeating TiN/Ti alloying layer in 0.5 mol/L H2SO4 solution was greatly increased and the corrosion rate was only 0.3082 g/m^2. h.展开更多
[ Objective] This study aimed to investigate enzymatic hydrolysis technology of glutinous rice and the oxidation resistance activity of the enzymatic hydrolysis solution. [ Method ] White glutinous rice was hydrolyzed...[ Objective] This study aimed to investigate enzymatic hydrolysis technology of glutinous rice and the oxidation resistance activity of the enzymatic hydrolysis solution. [ Method ] White glutinous rice was hydrolyzed using four kinds of proteases including neutral protease, alkaline protease, papain and trypsin. Using the scavenging rate of hydroxyl radical ( ·OH) as an indicator and appropriate protease as hydrolytic enzyme, the effects of protein substrate concentration, enzyme dosage, enzymatic hydrolysis temperature and initial pH on the abilities of proteases to scavenge hydroxyl radical from enzymatic hydrolysis solution of glutinous rice were investigated. Based on single-factor test, L9 (34) orthogonal experimental design was adopted, to determine the optimal enzymatic hydrolysis condi- tions leading to the highest oxidation resistance activity of enzymatic hydrolysis solution. [ Result] The optimized process parameters for enzymatic hydrolysis of glu- tinous rice protein with neutral protease were: protein substrate concentration of 2%, enzyme dosage of 24 000 U/g protein (protein meter), enzymatic hydrolysis temperature of 55 ℃, initial pH of 8.0, and enzymatic hydrolysis duration of 0.5 h; under these conditions, the hydroxyl radical scavenging rate could reach 56. 05% ; protein substrate concentration, enzyme dosage, enzymatic hydrolysis temperature and initial pH had extremely significant effects on the hydroxyl radical scavenging rate. In addition, the activities of antioxidant peptides in glutinous rice hydrolysates were well maintained within a temperature range of 60 - 100℃. [Condusion] The study produced theoretical feasibility reference for the production of functional base powder by spray drying.展开更多
We constructed small high-yielding binary Ti vectors with a bacterial tetracycline resistance gene to facilitate efficient cloning afforded by the Gateway Technology (Invitrogen) for Agrobacterium tumefaciens-mediated...We constructed small high-yielding binary Ti vectors with a bacterial tetracycline resistance gene to facilitate efficient cloning afforded by the Gateway Technology (Invitrogen) for Agrobacterium tumefaciens-mediated transformation of higher plants. The Gateway Technology vectors are kanamycin-based, thus tetracycline-based destination and expression vectors are easily selected for the antibiotic resistance in the Escherichia coli media. We reduced the size of the tetracycline resistance gene TetC from pBR322 to 1468 bp containing 1191 bp of the coding region, 93 bp of 5’-upstream, and 184 bp 3’-downstream region. The final size of binary Ti vector skeleton pLSU11 is 5034 bp. pLSU12 and 13 have the kanamycin resistance NPTII gene as a plant-selectable marker. pLSU13?and 15 contain the hygromycin resistance HPH gene as a selection marker. pLSU13 and 15 also have the β-glucuronidase (GUS) reporter gene in addition to the plant selection marker. We also constructed a mobilizable version of tetracycline-based binary Ti vector pLSU16 in which the mob function of ColE1 replicon was maintained for mobilization of the binary vector from E. coli to A. tumefaciens by tri-parental mating. The final size of binary Ti vector skeleton pLSU16 is 5580 bp. New tetracycline- based binary Ti vectors pLSU12 were found as effective as kanamycin-based vector pLSU2 in promoting a 10-fold increase in fresh weight yield of kanamycin-resistant calli after A. tumefaciens-mediated transformation of tobacco leaf discs. Using the Gateway Technology we introduced the plant-expressible GUSgene to the T-DNA of binary Ti vector pLSU12. Expression of the β-glucuronidase enzyme activity was demonstrated by histochemical staining of the GUS activity in transformed tobacco leaf discs.展开更多
In this paper, we have investigated the design parameters of RF CMOS switch, which will be used for the wireless tele-communication systems. A double-pole four-throw double-gate radio-frequency complementary-metal-oxi...In this paper, we have investigated the design parameters of RF CMOS switch, which will be used for the wireless tele-communication systems. A double-pole four-throw double-gate radio-frequency complementary-metal-oxide-semicon- ductor (DP4T DG RF CMOS) switch for operating at the 1 GHz is implemented with 45-nm CMOS process technology. This proposed RF switch is capable to select the data streams from the two antennas for both the transmitting and receiving processes. For the development of this DP4T DG RF CMOS switch we have explored the basic concept of the proposed switch circuit elements required for the radio frequency systems such as drain current, threshold voltage, resonant frequency, return loss, transmission loss, VSWR, resistances, capacitances, and switching speed.展开更多
Pyricularia oryzae anamorph of Magnaporthe oryzae is one of the most notorious fungal pathogens causing severe economic loss in rice production worldwide. Various methods, viz. cultural, biological and molecular appro...Pyricularia oryzae anamorph of Magnaporthe oryzae is one of the most notorious fungal pathogens causing severe economic loss in rice production worldwide. Various methods, viz. cultural, biological and molecular approaches, are utilized to counteract this pathogen. Moreover, some tolerant or resistant rice varieties have been developed with the help of breeding programmes. Isolation and molecular characterization of different blast resistance genes now open the gate for new possibilities to elucidate the actual allelic variants of these genes via various molecular breeding and transgenic approaches. However, the behavioral pattern of this fungus breakups the resistance barriers in the resistant or tolerant rice varieties. This host-pathogen barrier will be possibly countered in future research by comparative genomics data from available genome sequence data of rice and M. oryzae for durable resistance. Present review emphasized fascinating recent updates, new molecular breeding approaches, transgenic and genomics approaches(i.e. mi RNA and genome editing) for the management of blast disease in rice. The updated information will be helpful for the durable, resistance breeding programme in rice against blast pathogen.展开更多
(CoCrFeNi)95Nb5 high entropy alloy(HEA)coatings were successfully fabricated on a substrate of Q235 steel by laser cladding technology.These(CoCrFeNi)95Nb5 HEA coatings possess excellent properties,particularly corros...(CoCrFeNi)95Nb5 high entropy alloy(HEA)coatings were successfully fabricated on a substrate of Q235 steel by laser cladding technology.These(CoCrFeNi)95Nb5 HEA coatings possess excellent properties,particularly corrosion resistance,which is clearly superior to that of some typical bulk HEA and common engineering alloys.In order to obtain appropriate laser cladding preparation process parameters,the effects of laser energy density on the microstructure,microhardness,and corrosion resistance of(CoCrFeNi)95Nb5 HEA coating were closely studied.Results showed that as the laser energy density increases,precipitation of the Laves phase in(CoCrFeNi)95Nb5 HEA coating gradually decreases,and diffusion of the Fe element in the substrate intensifies,affecting the integrity of the(CoCrFeNi)95Nb5 HEA.This decreases the microhardness of(CoCrFeNi)95Nb5 HEA coatings.Moreover,the relative content of Cr2O3,Cr(OH)3,and Nb2O5 in the surface passive film of the coating decreases with increasing energy density,causing corrosion resistance to decrease.This study demonstrates the controllability of a high-performance HEA coating using laser cladding technology,which has significance for the laser cladding preparation of other CoCrFeNi-system HEA coatings.展开更多
In this paper, we describe some recent imaging technologies developed by Schlumberger for oilfield downhole mul- tiphase flow production logging (PL) and cross-well electromagnetic (EM) survey applications. FloScan Im...In this paper, we describe some recent imaging technologies developed by Schlumberger for oilfield downhole mul- tiphase flow production logging (PL) and cross-well electromagnetic (EM) survey applications. FloScan Imager (FSITM) has been introduced as a 3-phase oil/gas/water flow PL tool for deviated and horizontal wells. FSI sensors can map fluid velocity and holdup profiles along a vertical diameter of the wellbore at every survey depth, enabling a robust estimate of the individual phase flow rates in complex flow regimes. The cross-well EM survey is based on cross-borehole induction logging technique and provides resistivity distribution at a reservoir scale. It is a useful tool for reservoir management and is most effective in dynamic fields where fluid saturations are variable in time and space. The tool can be used to identify (water or steam) flooded and bypassed regions. By monitoring changes in the resistivity spatial distribution with time, cross-well EM survey is very effective at mapping inter-well temperature and structure. Some field examples are shown for both FloScan Imager PL tool and cross-well resistivity imaging survey.展开更多
As a new type of hull form,trimaran has remarkable excellent performances and has drawn more and more attention.When the viscous CFD technology now available is applied to the research of resistance performance of tri...As a new type of hull form,trimaran has remarkable excellent performances and has drawn more and more attention.When the viscous CFD technology now available is applied to the research of resistance performance of trimaran,the spatial discretization would usually result in the grid error and uncertainty,and thus the considerable discrepancy between the numerical results and the experimental data.In order to ascertain how much the grid would affect the calculation,the grid convergence should be studied.A mathematical trimaran was chosen as an example,with the commercial code CFX for the simulation,VOF for surface treatment,and the grid study was carried out based on two different turbulence models.It was concluded that carrying out grid study is helpful in estimating the grid error and uncertainty,and indicating the direction of improving the credibility of the numerical calculation, and,in addition to grid errors and uncertainties,the turbulence modeling errors and uncertainties contribute to the simulation errors.展开更多
Driving safety is of significance in the automobile industry and transportation systems. Pavement skid resistance has long been recognized as the most important parameter in reducing traffic accidents especially in we...Driving safety is of significance in the automobile industry and transportation systems. Pavement skid resistance has long been recognized as the most important parameter in reducing traffic accidents especially in wet conditions. The knowledge of the friction coefficient and skid resistance is very valuable information for safety enhancement of roads. Thus, it is important to find proper methods for measuring skid resistance and frictional properties of the pavement surface. There is a wide range of measurement methods and devices for measuring skid resistance. This paper presents a review on the research studies that have been done on characterization of the frictional properties of the pavement surface and discussed methods used for measurement and evaluation of texture characteristics and the strengths and weaknesses of these methods. Finally, some ideas have been suggested to develop new methods for better and proper measurement of skid resistance.展开更多
OBJECTIVE Chemotherapy is an important therapy for hepatocellular carcinoma (HCC). However, it is not effective in many cases due to recurrence and metastasis even if the initial treatment produces a response. Multi...OBJECTIVE Chemotherapy is an important therapy for hepatocellular carcinoma (HCC). However, it is not effective in many cases due to recurrence and metastasis even if the initial treatment produces a response. Multidrug resistance (MDR) is considered to be one of the considerable causes. The aim of this study was to reverse MDR of HepG2/ADM cells by blocking mdr1 with an adenovirus vector carrying antisense mdr1 in a tumor transplantated in athymic mice. METHODS PCMV IE was removed from the pshuttle vector. A 0.3 kb AFP promoter was inserted into the pshuttle vector and pCMV changed into pAFP. The pAFP and asmdr1 PCR products were doubly digested with Kpnl and Apal, the digested products were ligated by T4 ligase, the asmdr1 gene was inserted into pAFP and a newly plasmid pAFP-asmdr1 was constructed. Following digestion with PI-SceI/I-Ceu I, pAFP-asmdr1 was ligated with Adeno-X genome DNA and amplified in E.coli XL1-Blue. The HEK293 cells were transfected and virus collected. The HepG2 MDR cells (HepG2/ADM) were induced by graded resistance to ADM and were inoculated into athymic mice. After adeno-asmdr1 was injected, the expression of mdr1-mRNA and the volume of the transplantated tumor and its cells were observed. RESULTS Following injection with Adeno-asmdr1, the tumor volume in the ADM+Adeno-asmdr1 group did not increase. However the tumor volume in the PBS plus ADM group did significantly increase (P〈0.05). In the tumor xenograft cells, mdr1 mRNA in the xenografts was assessed by RT-PCR and was found to be reduced at 1 week and 4 weeks in the ADM+asmdr1 group, but it was stable in the ADM group. It was only 20% in the ADM+asmdr1 group compared to the ADM group at the 4th week (P〈0.05). Evidence of apoptosis was observed in the tumor xenograft cells treated with Adeno-asmdr1, but there was rare or no apoptosis in the group treated with ADM and PBS. CONCLUSION Adenovirus carrying antisense mdr1 RNA can partially reverse the MDR of HepG2/ADM cells and inhibit tumor growth by down-regulating mdr1 mRNA resulting in tumor cell apoptosis.展开更多
Ti/Al/Ti/Au and Ti/Al/Ni/Au ohmic contacts were fabricated on AlGaN/GaN heterostructure under different temperatures of rapid thermal processing (RTP). Since abnormal resistance values were observed during the conta...Ti/Al/Ti/Au and Ti/Al/Ni/Au ohmic contacts were fabricated on AlGaN/GaN heterostructure under different temperatures of rapid thermal processing (RTP). Since abnormal resistance values were observed during the contact resistance testing,the surface morphology and contact borders of the samples were analyzed to determine the physical mechanism. Such abnormal phenomenon is found to originate from cracking of the AlGaN layer during RTP,flowing of Ti/Al metallic liquid along the crevices,and continuous reaction of the metallic liquid with AlGaN/GaN. Such processes result in abnormal conduction channels. The possible mechanism of the crevice formation was discussed,and the possible solutions to avoid the crevices were proposed.展开更多
Low-tempemture stress is a major abiotic stress influencing plant growth, economic yield and quality worldwide. Precise and reliable identification and test of plant cold resistance is a foundation for exploring low-t...Low-tempemture stress is a major abiotic stress influencing plant growth, economic yield and quality worldwide. Precise and reliable identification and test of plant cold resistance is a foundation for exploring low-temperature damages, cold resistance mechanism of plants, cultivating and innovating quality cold-re- sistant germplasm resources, which is rather important for woody plants because their perennial and large-sized characteristics. Generally, most researches on plant cold-resistant physiology focused on identification and utilization of cold resistance worldwide, and a few on test methods of cold resistance, but cold resistance mechanism has been less reported. Therefore, researches on methods of identifying and testing plant resistance have achieved considerable progress, accumulated much experience and formed characteristics gradually in terms of methodology. Current methods of identifying plant cold resistance include open field/outdoor culti- vation evaluation, cold and frost damage investigation, simulated cold weather, and mathematical model forecast. And the methods of testing plant cold resistance include growth condition test, frozen index investigation, electrolyte leakage, bioelectrical impedance mapping and so on. Synthesizing the methods of identifying and testing plant cold resistance should be a key matter in the present and future researches and application of plant cold resistance physiology, improvement and in- novation of the identification and test will be an important direction of the future researches. Identification and test of plant cold resistance based on genomies, meta- bonomics and proteomics should be the new trend of the researches on physiology and ecology of plant cold resistance.展开更多
Low power consumption is a major issue in nowadays electronics systems. This trend is pushed by the development of data center related to cloud services and soon to the Internet of Things (IoT) deployment. Memories ...Low power consumption is a major issue in nowadays electronics systems. This trend is pushed by the development of data center related to cloud services and soon to the Internet of Things (IoT) deployment. Memories are one of the major contributors to power consumption. However, the development of emerging memory technologies paves the way to low-power design, through the partial replacement of the dynamic random access memory (DRAM) with the non-volatile stand-alone memory in servers or with the embedded or distributed emerging non-volatile memory in IoT objects. In the latter case, non-volatile flip-flops (NVFFs) seem a promising candidate to replace the retention latch. Indeed, IoT objects present long sleep time and NVFFs offer to save data in registers with zero power when the application is idle. This paper gives an overview of NVFF architecture flavors for various emerging memory technologies.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.42307189 and 42030701)the China Postdoctoral Science Foundation(Grant No.2023M740974).
文摘The actively heated fiber-optic(AHFO)technology has become emerged as a research focus due to its advantages of distributed,real-time measurement and good durability.These attributes have led to the gradual application of AHFO technology to the water content measurement of in situ soil.However,all existing in situ applications of AHFO technology fail to consider the effect of soilesensor contact quality on water content measurements,limiting potential for the wider application of AHFO technology.To address this issue,the authors propose a method for determining the soilesensor thermal contact resistance based on the principle of an infinite cylindrical heat source.This is then used to establish an AHFO water content measurement technology that considers the thermal contact resistance.The reliability and validity of the new measurement technology are explored through a laboratory test and a field case study,and the spatial-temporal evolution of the soil water content in the case is revealed.The results demonstrate that method for determining the soilesensor thermal contact resistance is highly effective and applicable to all types of soils.This method requires only the moisture content,dry density,and thermal response of the in situ soil to be obtained.In the field case,the measurement error of soil water content between the AHFO method,which takes into account the thermal contact resistance,and the neutron scattering method is only 0.011.The water content of in situ soil exhibits a seasonal variation,with an increase in spring and autumn and a decrease in summer and winter.Furthermore,the response of shallow soils to precipitation and evaporation is significant.These findings contribute to the enhancement of the accuracy of the AHFO technology in the measurement of the water content of in situ soils,thereby facilitating the dissemination and utilization of this technology.
文摘Based on the features and requirements of gas drainage system, an optimized explosion resistance technology is done after a comprehensive analysis and research about the triple IR (Infrared Ray) flame detection technology, explosion resistance valve technology and explosion resistance control technology. An intelligent PLC (Programmable Logic Controller) resistance control system is designed which can cut offthe gas branch quickly and accurately, and the controller have automatic pressure maintaining function, valve rotation limit function, remote and local control interlock function. The reliability and rationality of explosion resistance technology is verified by gas pipeline explosion propagation and resistance simulation test. Overall response time of explosion resistance system is less than 100ms, and the spread of fire in gas pipeline can be prevented effectively.
文摘Founded in 2012,the National Joint Engineering Research Center of High Performance Metal Wear Resistant Materials Technology at Jinan University,one of the'211'key national universities in China,specializes in the research and development of iron based wear resistant materials and their casting technologies to provide support to the production process.The Research Center serves the'Guangdong Province Ceeusro Innovation Platform for Common Technology of High Performance Wear Resistant Materials”,“Guangdong Province Engineering Research Center for Wear Resistant and Special Functional Materials”.
文摘One of the major challenges in designing and fabricating Spintronic devices is the choice of both, Materials and the Technology, along with understanding the intricacies of the Designing aspects. In this communication, we have attempted to briefly discuss these factors, with an aim to draw the attention of the Materials Scientists and Technologists to this serious challenge, in the direction of which, though a lot of research and development work has been done, still needs more concerted efforts to be made in order to make the Spintronic devices that can offer good efficiency for maximizing their usefulness.
基金supported by National Natural Science Foundation of China (No. 50374054)the Natural Science Foundation of Shanxi Province (No. 20031050)
文摘TiN/Ti multi-permeating alloying layer has been formed on the low carbon steel by means of the double glow-discharge plasma surface alloying technique and hollow-cathode effect. The alloying layer was detected by axiovert 25 CA optical microscope with computer analyzing software (LEC), GDA-2 glow discharge spectroscopy (GDS), X-ray diffraction (XRD) and galvanochemical method. The results showed that the thickness of TiN/Ti multi-permeating alloying layer was about 10μm, the content of Ti on the surface was up to 63.48 wt% and the content of N was up to 12.46 wt%. The atom Ti and N concentrations changed gradually across the depth of the alloying layer and the preferred orientation of TiN/Ti alloying layer was crystal surface (200). The multi-permeating alloying layer and substrate were combined through metallurgy. The surface appearances of the multi-permeating alloying layer were uniform and of a compact cellular structure. The hardness of the surface was about 1600-3000 HV0.1. The corrosion resistance of the permeating TiN/Ti alloying layer in 0.5 mol/L H2SO4 solution was greatly increased and the corrosion rate was only 0.3082 g/m^2. h.
基金Supported by Education Reform Project of Jiangxi Province(No.JXJG-20084-27)
文摘[ Objective] This study aimed to investigate enzymatic hydrolysis technology of glutinous rice and the oxidation resistance activity of the enzymatic hydrolysis solution. [ Method ] White glutinous rice was hydrolyzed using four kinds of proteases including neutral protease, alkaline protease, papain and trypsin. Using the scavenging rate of hydroxyl radical ( ·OH) as an indicator and appropriate protease as hydrolytic enzyme, the effects of protein substrate concentration, enzyme dosage, enzymatic hydrolysis temperature and initial pH on the abilities of proteases to scavenge hydroxyl radical from enzymatic hydrolysis solution of glutinous rice were investigated. Based on single-factor test, L9 (34) orthogonal experimental design was adopted, to determine the optimal enzymatic hydrolysis condi- tions leading to the highest oxidation resistance activity of enzymatic hydrolysis solution. [ Result] The optimized process parameters for enzymatic hydrolysis of glu- tinous rice protein with neutral protease were: protein substrate concentration of 2%, enzyme dosage of 24 000 U/g protein (protein meter), enzymatic hydrolysis temperature of 55 ℃, initial pH of 8.0, and enzymatic hydrolysis duration of 0.5 h; under these conditions, the hydroxyl radical scavenging rate could reach 56. 05% ; protein substrate concentration, enzyme dosage, enzymatic hydrolysis temperature and initial pH had extremely significant effects on the hydroxyl radical scavenging rate. In addition, the activities of antioxidant peptides in glutinous rice hydrolysates were well maintained within a temperature range of 60 - 100℃. [Condusion] The study produced theoretical feasibility reference for the production of functional base powder by spray drying.
文摘We constructed small high-yielding binary Ti vectors with a bacterial tetracycline resistance gene to facilitate efficient cloning afforded by the Gateway Technology (Invitrogen) for Agrobacterium tumefaciens-mediated transformation of higher plants. The Gateway Technology vectors are kanamycin-based, thus tetracycline-based destination and expression vectors are easily selected for the antibiotic resistance in the Escherichia coli media. We reduced the size of the tetracycline resistance gene TetC from pBR322 to 1468 bp containing 1191 bp of the coding region, 93 bp of 5’-upstream, and 184 bp 3’-downstream region. The final size of binary Ti vector skeleton pLSU11 is 5034 bp. pLSU12 and 13 have the kanamycin resistance NPTII gene as a plant-selectable marker. pLSU13?and 15 contain the hygromycin resistance HPH gene as a selection marker. pLSU13 and 15 also have the β-glucuronidase (GUS) reporter gene in addition to the plant selection marker. We also constructed a mobilizable version of tetracycline-based binary Ti vector pLSU16 in which the mob function of ColE1 replicon was maintained for mobilization of the binary vector from E. coli to A. tumefaciens by tri-parental mating. The final size of binary Ti vector skeleton pLSU16 is 5580 bp. New tetracycline- based binary Ti vectors pLSU12 were found as effective as kanamycin-based vector pLSU2 in promoting a 10-fold increase in fresh weight yield of kanamycin-resistant calli after A. tumefaciens-mediated transformation of tobacco leaf discs. Using the Gateway Technology we introduced the plant-expressible GUSgene to the T-DNA of binary Ti vector pLSU12. Expression of the β-glucuronidase enzyme activity was demonstrated by histochemical staining of the GUS activity in transformed tobacco leaf discs.
文摘In this paper, we have investigated the design parameters of RF CMOS switch, which will be used for the wireless tele-communication systems. A double-pole four-throw double-gate radio-frequency complementary-metal-oxide-semicon- ductor (DP4T DG RF CMOS) switch for operating at the 1 GHz is implemented with 45-nm CMOS process technology. This proposed RF switch is capable to select the data streams from the two antennas for both the transmitting and receiving processes. For the development of this DP4T DG RF CMOS switch we have explored the basic concept of the proposed switch circuit elements required for the radio frequency systems such as drain current, threshold voltage, resonant frequency, return loss, transmission loss, VSWR, resistances, capacitances, and switching speed.
文摘Pyricularia oryzae anamorph of Magnaporthe oryzae is one of the most notorious fungal pathogens causing severe economic loss in rice production worldwide. Various methods, viz. cultural, biological and molecular approaches, are utilized to counteract this pathogen. Moreover, some tolerant or resistant rice varieties have been developed with the help of breeding programmes. Isolation and molecular characterization of different blast resistance genes now open the gate for new possibilities to elucidate the actual allelic variants of these genes via various molecular breeding and transgenic approaches. However, the behavioral pattern of this fungus breakups the resistance barriers in the resistant or tolerant rice varieties. This host-pathogen barrier will be possibly countered in future research by comparative genomics data from available genome sequence data of rice and M. oryzae for durable resistance. Present review emphasized fascinating recent updates, new molecular breeding approaches, transgenic and genomics approaches(i.e. mi RNA and genome editing) for the management of blast disease in rice. The updated information will be helpful for the durable, resistance breeding programme in rice against blast pathogen.
基金This work was financially supported by the National Key R&D Program of China(No.2018YFB0606104)the National Natural Science Foundation of China(No.51702332).
文摘(CoCrFeNi)95Nb5 high entropy alloy(HEA)coatings were successfully fabricated on a substrate of Q235 steel by laser cladding technology.These(CoCrFeNi)95Nb5 HEA coatings possess excellent properties,particularly corrosion resistance,which is clearly superior to that of some typical bulk HEA and common engineering alloys.In order to obtain appropriate laser cladding preparation process parameters,the effects of laser energy density on the microstructure,microhardness,and corrosion resistance of(CoCrFeNi)95Nb5 HEA coating were closely studied.Results showed that as the laser energy density increases,precipitation of the Laves phase in(CoCrFeNi)95Nb5 HEA coating gradually decreases,and diffusion of the Fe element in the substrate intensifies,affecting the integrity of the(CoCrFeNi)95Nb5 HEA.This decreases the microhardness of(CoCrFeNi)95Nb5 HEA coatings.Moreover,the relative content of Cr2O3,Cr(OH)3,and Nb2O5 in the surface passive film of the coating decreases with increasing energy density,causing corrosion resistance to decrease.This study demonstrates the controllability of a high-performance HEA coating using laser cladding technology,which has significance for the laser cladding preparation of other CoCrFeNi-system HEA coatings.
文摘In this paper, we describe some recent imaging technologies developed by Schlumberger for oilfield downhole mul- tiphase flow production logging (PL) and cross-well electromagnetic (EM) survey applications. FloScan Imager (FSITM) has been introduced as a 3-phase oil/gas/water flow PL tool for deviated and horizontal wells. FSI sensors can map fluid velocity and holdup profiles along a vertical diameter of the wellbore at every survey depth, enabling a robust estimate of the individual phase flow rates in complex flow regimes. The cross-well EM survey is based on cross-borehole induction logging technique and provides resistivity distribution at a reservoir scale. It is a useful tool for reservoir management and is most effective in dynamic fields where fluid saturations are variable in time and space. The tool can be used to identify (water or steam) flooded and bypassed regions. By monitoring changes in the resistivity spatial distribution with time, cross-well EM survey is very effective at mapping inter-well temperature and structure. Some field examples are shown for both FloScan Imager PL tool and cross-well resistivity imaging survey.
文摘As a new type of hull form,trimaran has remarkable excellent performances and has drawn more and more attention.When the viscous CFD technology now available is applied to the research of resistance performance of trimaran,the spatial discretization would usually result in the grid error and uncertainty,and thus the considerable discrepancy between the numerical results and the experimental data.In order to ascertain how much the grid would affect the calculation,the grid convergence should be studied.A mathematical trimaran was chosen as an example,with the commercial code CFX for the simulation,VOF for surface treatment,and the grid study was carried out based on two different turbulence models.It was concluded that carrying out grid study is helpful in estimating the grid error and uncertainty,and indicating the direction of improving the credibility of the numerical calculation, and,in addition to grid errors and uncertainties,the turbulence modeling errors and uncertainties contribute to the simulation errors.
文摘Driving safety is of significance in the automobile industry and transportation systems. Pavement skid resistance has long been recognized as the most important parameter in reducing traffic accidents especially in wet conditions. The knowledge of the friction coefficient and skid resistance is very valuable information for safety enhancement of roads. Thus, it is important to find proper methods for measuring skid resistance and frictional properties of the pavement surface. There is a wide range of measurement methods and devices for measuring skid resistance. This paper presents a review on the research studies that have been done on characterization of the frictional properties of the pavement surface and discussed methods used for measurement and evaluation of texture characteristics and the strengths and weaknesses of these methods. Finally, some ideas have been suggested to develop new methods for better and proper measurement of skid resistance.
基金This work was supported by the Key Program of Medical Science Foundation of Chongqing Public Health Bureau (No. [2001]01-1-018).
文摘OBJECTIVE Chemotherapy is an important therapy for hepatocellular carcinoma (HCC). However, it is not effective in many cases due to recurrence and metastasis even if the initial treatment produces a response. Multidrug resistance (MDR) is considered to be one of the considerable causes. The aim of this study was to reverse MDR of HepG2/ADM cells by blocking mdr1 with an adenovirus vector carrying antisense mdr1 in a tumor transplantated in athymic mice. METHODS PCMV IE was removed from the pshuttle vector. A 0.3 kb AFP promoter was inserted into the pshuttle vector and pCMV changed into pAFP. The pAFP and asmdr1 PCR products were doubly digested with Kpnl and Apal, the digested products were ligated by T4 ligase, the asmdr1 gene was inserted into pAFP and a newly plasmid pAFP-asmdr1 was constructed. Following digestion with PI-SceI/I-Ceu I, pAFP-asmdr1 was ligated with Adeno-X genome DNA and amplified in E.coli XL1-Blue. The HEK293 cells were transfected and virus collected. The HepG2 MDR cells (HepG2/ADM) were induced by graded resistance to ADM and were inoculated into athymic mice. After adeno-asmdr1 was injected, the expression of mdr1-mRNA and the volume of the transplantated tumor and its cells were observed. RESULTS Following injection with Adeno-asmdr1, the tumor volume in the ADM+Adeno-asmdr1 group did not increase. However the tumor volume in the PBS plus ADM group did significantly increase (P〈0.05). In the tumor xenograft cells, mdr1 mRNA in the xenografts was assessed by RT-PCR and was found to be reduced at 1 week and 4 weeks in the ADM+asmdr1 group, but it was stable in the ADM group. It was only 20% in the ADM+asmdr1 group compared to the ADM group at the 4th week (P〈0.05). Evidence of apoptosis was observed in the tumor xenograft cells treated with Adeno-asmdr1, but there was rare or no apoptosis in the group treated with ADM and PBS. CONCLUSION Adenovirus carrying antisense mdr1 RNA can partially reverse the MDR of HepG2/ADM cells and inhibit tumor growth by down-regulating mdr1 mRNA resulting in tumor cell apoptosis.
文摘Ti/Al/Ti/Au and Ti/Al/Ni/Au ohmic contacts were fabricated on AlGaN/GaN heterostructure under different temperatures of rapid thermal processing (RTP). Since abnormal resistance values were observed during the contact resistance testing,the surface morphology and contact borders of the samples were analyzed to determine the physical mechanism. Such abnormal phenomenon is found to originate from cracking of the AlGaN layer during RTP,flowing of Ti/Al metallic liquid along the crevices,and continuous reaction of the metallic liquid with AlGaN/GaN. Such processes result in abnormal conduction channels. The possible mechanism of the crevice formation was discussed,and the possible solutions to avoid the crevices were proposed.
基金Supported by the National Natural Science Fundation of China(No.31270674)
文摘Low-tempemture stress is a major abiotic stress influencing plant growth, economic yield and quality worldwide. Precise and reliable identification and test of plant cold resistance is a foundation for exploring low-temperature damages, cold resistance mechanism of plants, cultivating and innovating quality cold-re- sistant germplasm resources, which is rather important for woody plants because their perennial and large-sized characteristics. Generally, most researches on plant cold-resistant physiology focused on identification and utilization of cold resistance worldwide, and a few on test methods of cold resistance, but cold resistance mechanism has been less reported. Therefore, researches on methods of identifying and testing plant resistance have achieved considerable progress, accumulated much experience and formed characteristics gradually in terms of methodology. Current methods of identifying plant cold resistance include open field/outdoor culti- vation evaluation, cold and frost damage investigation, simulated cold weather, and mathematical model forecast. And the methods of testing plant cold resistance include growth condition test, frozen index investigation, electrolyte leakage, bioelectrical impedance mapping and so on. Synthesizing the methods of identifying and testing plant cold resistance should be a key matter in the present and future researches and application of plant cold resistance physiology, improvement and in- novation of the identification and test will be an important direction of the future researches. Identification and test of plant cold resistance based on genomies, meta- bonomics and proteomics should be the new trend of the researches on physiology and ecology of plant cold resistance.
基金supported by the ANR project DIPMEM under Grant No.ANR-12-NANO-0010-04
文摘Low power consumption is a major issue in nowadays electronics systems. This trend is pushed by the development of data center related to cloud services and soon to the Internet of Things (IoT) deployment. Memories are one of the major contributors to power consumption. However, the development of emerging memory technologies paves the way to low-power design, through the partial replacement of the dynamic random access memory (DRAM) with the non-volatile stand-alone memory in servers or with the embedded or distributed emerging non-volatile memory in IoT objects. In the latter case, non-volatile flip-flops (NVFFs) seem a promising candidate to replace the retention latch. Indeed, IoT objects present long sleep time and NVFFs offer to save data in registers with zero power when the application is idle. This paper gives an overview of NVFF architecture flavors for various emerging memory technologies.