Comparison of Treatment Efficacy of Different Number of Botulinum Toxin Type A Injection Sites for Crow’s Feet Lines: A Single-Center Retrospective Clinical Study in Vietnam

Objective:Botulinum toxin type A is frequently used to treat crow’s feet lines.However,the optimal dose and injection sites are still controversial.The objective of this study was to compare the efficacy of different botulinum toxin type A injection patterns for the treatment of crow’s feet.Methods:This single-center,retrospective,clinical study was conducted at the National Hospital of Dermatology and Venereology from July 2020 to December 2020.Data on 60 patients with crow’s feet were collected and divided into 2 groups(3-or 4-point intramuscular injection)according to the intramuscular injection technique the patients received.The treatment efficacy was assessed based on the changes in the Crow’s Feet Grading Scale score at 1,4,and 16 weeks after treatment.Repeated measures analysis of variance was used for the assessment of changes in scores over time between the two groups.Results:After treatment,the average Crow’s Feet Grading Scale score was significantly decreased compared with the pretreatment score at all timepoints(1,4,and 16 weeks)in both states(dynamic:For 3-point intramuscular injection technique,1 week:1.90±0.71 vs.2.97±0.56;4 weeks:1.87±0.68 vs.2.97±0.56;16 weeks:2.60±0.67 vs.2.97±0.56.For 4-point intramuscular injection technique,1 week:1.73±0.83 vs.3.03±0.49;4 weeks:1.73±0.74 vs.3.03±0.49;16 weeks:2.57±0.68 vs.3.03±0.49,all P<0.001.and static:For 3-point intramuscular injection technique,1 week:1.20±0.89 vs.2.20±0.85;4 weeks:1.20±0.89 vs.2.20±0.85;16 weeks:1.87±0.97 vs.2.20±0.85;For 4-point intramuscular injection technique,1 week:1.50±0.86 vs.2.30±0.84;4 weeks:1.33±0.84 vs.2.30±0.84;16 weeks:1.87±0.97 vs.2.30±0.84.All P<0.001).The average subjective patient-rated satisfaction scores after treatment were significantly higher in the 4-point injection group than in the 3-point injection group(P=0.028).The adverse events were post-injection bruising in 3 patients and a feeling of eyelid tightness in 3 patients.Conclusion:Botulinum toxin type A injection is an effective treatment for crow’s feet.Adding a fourth injection maintains the same therapeutic effect and does not increase adverse effects.Patients with lower-fan crow’s feet patterns may benefit more from 4-point injection therapy.However,the present findings require confirmation in studies with larger sample sizes,longer follow-up times,and different botulinum toxin type A doses.

A Single Arm, Monocentric, Prospective, Open Label Study to Evaluate Anti-Ageing Efficacy of Human Growth Factors and Cytokines Containing Cosmetic Skin Serum in Indian Female Population

Introduction: The use of growth factors and cytokines in skin rejuvenation and reversal of photo ageing is a novel anti-ageing treatment. These factors provide a microenvironment that seems to favor tissue repair and regeneration. Methods: An open label, monocentric, single arm study to evaluate anti-ageing efficacy of conditioned medium obtained from adult human bone-marrow derived allogenic mesenchymal stromal cells (BMMSC) along with excipients formulated into a cosmetic serum, was conducted in 40 Indian female population aged 35 to 60 years, belonging to skin type IV - VI of Norwood Scale for a duration of 90 days. Parameters—fine lines, wrinkles, crow’s feet, evenness of skin tone, skin firmness/laxity, hydration of skin, homogeneity of age spots, and visible pores were used to evaluate the anti-ageing and rejuvenating properties of the test product. Results and Discussion: Improvement was seen in majority of parameters starting from 15 - 30 days. Product was safe and well tolerated as per the dermatologist and subjects’ self-assessment.