Cryopreservation of a Small Number of Human Sperm within Empty Zona Pellucidae

Objective To investigate the empty zona pellucida for use in the cryopreservation of human sperm. Materials & Methods Human and hamster zona pellucidae were evacuated and injected with testicular, epididymal and ejaculated sperm. The zona pellucidae with sperm were cryopreserved. Results After thawing, zona pellucidae were easily found, and sperm inside zona pellucidae were also easily observed. There were no differences in post-thaw motility and vitality between ejaculated and epididymal sperm groups (P>0.05), but these two parameters were lowered in testicular sperm group compared to both ejaculated and epididymal sperm (P<0.01). No significant difference was observed in post-thaw motilities among 6%, 7.5%, 9% glycerol concentrations (P>0.05). In addition, obvious differences in post-thaw motilities were not found between human and hamster empty zona pellucidae (P>0.05). Conclusion An evacuated zona pellucida is an ideal vehicle for the cryopreservation of a small number of human sperm.

少量人类精子以空卵透明带为冻贮载体的冷冻保存

目的:探讨空卵透明带作贮存载体冷冻保存少量人类精子。方法:将人或金黄地鼠卵内的细胞成份全部除去,制备成空卵透明带,分别显微注入人睾丸精子、附睾精子和射出精子后冷冻保存,并与正常供精者射出精子作对照。结果:解冻后卵透明带在溶液中容易识别寻找,卵透明带内的精子易于观察。附睾精子组(n=11)与供精者射出精子组(n=7)的冷冻精子活动率和存活率无显著性差异(P>0.05),但睾丸精子组(n=7)这两项参数值均显著低于附睾精子和射出精子组(P<0.01)。含6％、7.5％和9％不同甘油浓度的冷冻保护剂,对冷冻精子活动率无显著性影响(P>0.05);冻贮于人和金黄地鼠空卵透明带内的精子,两者的冷冻精子活动率也无显著性差异(P>0.05〕。结论:空卵透明带是冻贮少量人类精子的合适载体。

稀少精子冷冻研究进展

精子冷冻是人类辅助生殖领域中日益成熟的技术,但是常规精子冷冻方案却不适用于稀少精子冷冻,解决稀少精子冷冻技术将在很大程度上改善临床治疗少、弱精子症和无精子症患者的不育问题。寻找合适冷冻载体是目前稀少精子冷冻的研究重点之一,本文就近年来稀少精子不同载体冷冻方法:空卵膜载体冷冻法、微滴冷冻法、其他微型载体冷冻法和睾丸组织冷冻以及睾丸精子和附睾精子的冷冻效果作一综述。