Effect of oil pollution on gluthione and relative enzyme in oyster (Saccostrea cuculiata)

The oysters ( Saccostrea cuculiata ) were collected from four stations around Xiamen Island (Lundu Port, Xinglin Bay, Tong" an Bay, Huangcuo). The relation between the level of petroleum hydrocarbon in whole tissue and the contents of glutathione (GSH), the activity of selenium-dependent glutathione peroxidase (Se-GPx) and glutathione S-transferase (GST) in digestive gland and gill were investigated. The results showed: (1) The contents of petroleum hydrocarbon in oyster collected from four stations (Lundu Port, Xinglin Bay, Tong'an Bay, Huangcuo) were 380.68, 112.34, 27.31, 20.37μg/g wet weight, respectively; (2) the activity of Se-GPx and GST in digestive gland was lower than that in gill, and the content of GSH seemed reversibly; (3) among the four stations, both Se-GPx and GST activity of digestive gland and gill in Saccostrea cuculiata sampled from the four stations showed a good correlation with whole tissue petroleum hydrocarbon, could be as biomarkers of sea oil pollution.

石油污染对僧帽牡蛎(Ostrea cucullata)抗氧化酶的影响

在厦门岛及附近海域的轮渡码头、杏林湾、同安湾、黄厝 4个地点现场采集牡蛎样品 ,研究牡蛎全组织石油烃含量与其消化腺、鳃超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)活性之间的关系 ,并探讨了将僧帽牡蛎抗氧化酶作为监测海洋石油污染的生物标志物 (biomarker)的可能性 .结果表明 :(1)采自轮渡码头、杏林湾、同安湾、黄厝的牡蛎全组织石油烃含量分别为380 6 8、112 34、2 7 31、2 0 37μg g ;(2 )牡蛎消化腺SOD、CAT活性均高于鳃 ;(3)牡蛎消化腺和鳃CAT、SOD活性均随石油烃含量的增加而增强 ,由相关系数来判断两者相关性显著 。