In cucumber, fruit shape is an important quality criterion, and fruit bending is known to limit growth, yield, and taste. To investigate the post-transcriptional changes that regulate fruit bending and to better under...In cucumber, fruit shape is an important quality criterion, and fruit bending is known to limit growth, yield, and taste. To investigate the post-transcriptional changes that regulate fruit bending and to better understand the underlying molecular mechanisms, we generated a proteomic profile of the abdomen and back of cucumber bending fruit. Two-dimensional gel electrophoresis （2-DE） allowed the detection of approximately 900 distinct protein spots in each gel, 32 of which were differentially expressed in the abdomen and back of bending cucumber fruit. Ten of the differentially expressed proteins were analyzed using matrix-assisted laser ionization time of flight mass spectrometry （MALDI-TOF/MS）. A search of primary databases showed that the identified proteins are involved in various metabolic processes and cellular responses, including photosynthesis metabolism, energy metabolism, defense and stress response, and regulation. The identified proteins included large subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase, which are involved in photosynthesis and photorespiratory metabolism, and isocitrate dehydrogenase, which is involved in the tricarboxylic acid cycle. It is possible that imbalances in catabolic and anabolic processes directly affect the bending of cucumber fruit. The predicted function of the cobalamin- independent methionine synthase isozyme is closely related to ethylene biosynthesis; fruit bending may be regulated by ethylene, or by ethylene signaling crosstalk during fruit development. The 14-3-3 protein is usually considered to be a regulation-related protein, which plays a role in regulating cell hyperplasia, cell differentiation during growth, and apoptosis during senescence. Involvement of guanosine triphosphate （GTP）-binding proteins in signal transmission is known to regulate the development of cells in cucumber fruits and to play a role in fruit shape variation. Patterns of protein expression showed high repeatability. We hypothesize that these proteins may play an important role in growth and bending of cucumber fruits. The results of our study provide insight into the genetic mechanism underlying fruit bending in cucumber, and may help to promote cultivation of new varieties with superior fruits.展开更多
Nitrogen deficiency is a major factor that affects the yield and quality of horticultural crops.Differentially expressed proteins were identified in cucumber fruit under nitrogen deficiency treatment,and are expected ...Nitrogen deficiency is a major factor that affects the yield and quality of horticultural crops.Differentially expressed proteins were identified in cucumber fruit under nitrogen deficiency treatment,and are expected to be highly useful for dissecting carbon,nitrogen and ascorbate metabolism in cucumber and for improving nitrogen fertilizer utilization.Cucumber plants grown hydroponically in Hoagland solution were transferred to nitrogen-free conditions for 3 or 5 d after the blossom of female flowers.Fruit proteome profiles were assessed using twodimensional electrophoresis.The relative expression of the genes encoding 50 selected differentially expressed proteins and 4 key enzymes in carbon metabolism was analyzed by qRT-PCR.Resulted showed that most of the identified differentially expressed proteins were mainly associated with carbon metabolism,amino acid biosynthesis,ascorbate metabolism,and proteasomes.Glucose phosphorylation was enhanced,while the pentose phosphate pathway was inhibited.Carbon metabolism and the synthesis of most amino acids was affected significantly,and ascorbate might be accumulated under nitrogen deficiency in cucumber fruit.展开更多
Bent varieties and straight varieties were made as parents for the genetic analysis to investigate cucumber bending genetic mechanism. The results showed that the bent characters of the cucumber fruit （BCCF） were qu...Bent varieties and straight varieties were made as parents for the genetic analysis to investigate cucumber bending genetic mechanism. The results showed that the bent characters of the cucumber fruit （BCCF） were quantitative inheritance controlled by multiple genes and major genes. The additive effect played the main role and the dominance effect played the lesser role. Compared with the additive environmental variance, the dominant-environmental variance was more important and the cucumber fruit was more easily affected by the additive effect. The broad heritability and the narrow heritability of BCCF were both higher. The varieties of D0455 and D07299 could be used as parents which were benefit for improving the straight characters of the cucumber fruit展开更多
基金financially supported by the National High-Tech R&D Program of China (863 Program, 2006AA10Z1B9)the National Natural Science Foundation of China (NSFC, 31140058)the Program of Innovative Research Team for the Higher Education Institutions of Heilongjiang Province, China (2009td07)
文摘In cucumber, fruit shape is an important quality criterion, and fruit bending is known to limit growth, yield, and taste. To investigate the post-transcriptional changes that regulate fruit bending and to better understand the underlying molecular mechanisms, we generated a proteomic profile of the abdomen and back of cucumber bending fruit. Two-dimensional gel electrophoresis （2-DE） allowed the detection of approximately 900 distinct protein spots in each gel, 32 of which were differentially expressed in the abdomen and back of bending cucumber fruit. Ten of the differentially expressed proteins were analyzed using matrix-assisted laser ionization time of flight mass spectrometry （MALDI-TOF/MS）. A search of primary databases showed that the identified proteins are involved in various metabolic processes and cellular responses, including photosynthesis metabolism, energy metabolism, defense and stress response, and regulation. The identified proteins included large subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase, which are involved in photosynthesis and photorespiratory metabolism, and isocitrate dehydrogenase, which is involved in the tricarboxylic acid cycle. It is possible that imbalances in catabolic and anabolic processes directly affect the bending of cucumber fruit. The predicted function of the cobalamin- independent methionine synthase isozyme is closely related to ethylene biosynthesis; fruit bending may be regulated by ethylene, or by ethylene signaling crosstalk during fruit development. The 14-3-3 protein is usually considered to be a regulation-related protein, which plays a role in regulating cell hyperplasia, cell differentiation during growth, and apoptosis during senescence. Involvement of guanosine triphosphate （GTP）-binding proteins in signal transmission is known to regulate the development of cells in cucumber fruits and to play a role in fruit shape variation. Patterns of protein expression showed high repeatability. We hypothesize that these proteins may play an important role in growth and bending of cucumber fruits. The results of our study provide insight into the genetic mechanism underlying fruit bending in cucumber, and may help to promote cultivation of new varieties with superior fruits.
基金This research was funded by the National Key Researchand Development Program of China(2017YFE0118500)the National Natural Science Foundation of China(Grant No.31672203).
文摘Nitrogen deficiency is a major factor that affects the yield and quality of horticultural crops.Differentially expressed proteins were identified in cucumber fruit under nitrogen deficiency treatment,and are expected to be highly useful for dissecting carbon,nitrogen and ascorbate metabolism in cucumber and for improving nitrogen fertilizer utilization.Cucumber plants grown hydroponically in Hoagland solution were transferred to nitrogen-free conditions for 3 or 5 d after the blossom of female flowers.Fruit proteome profiles were assessed using twodimensional electrophoresis.The relative expression of the genes encoding 50 selected differentially expressed proteins and 4 key enzymes in carbon metabolism was analyzed by qRT-PCR.Resulted showed that most of the identified differentially expressed proteins were mainly associated with carbon metabolism,amino acid biosynthesis,ascorbate metabolism,and proteasomes.Glucose phosphorylation was enhanced,while the pentose phosphate pathway was inhibited.Carbon metabolism and the synthesis of most amino acids was affected significantly,and ascorbate might be accumulated under nitrogen deficiency in cucumber fruit.
基金Supported by 863 Projects (2006AA10Z1B9)Heilongjiang Science and Technology Innovation Team Project (2009td07)
文摘Bent varieties and straight varieties were made as parents for the genetic analysis to investigate cucumber bending genetic mechanism. The results showed that the bent characters of the cucumber fruit （BCCF） were quantitative inheritance controlled by multiple genes and major genes. The additive effect played the main role and the dominance effect played the lesser role. Compared with the additive environmental variance, the dominant-environmental variance was more important and the cucumber fruit was more easily affected by the additive effect. The broad heritability and the narrow heritability of BCCF were both higher. The varieties of D0455 and D07299 could be used as parents which were benefit for improving the straight characters of the cucumber fruit
