Cultural Diversity of Traditional Settlement Landscape Gene

As a crucial channel for understanding traditional settlements culture,traditional settlement landscape gene is of vital importance to figure out status quo of traditional Chinese culture,to protect cultural heritage of settlements,and to help residents establish cultural confidence.This paper adopts diversity index,schema analysis,and qualitative analysis methods to identify cultural diversity and construct diversity map of cultural landscape genes.It shows that cultural diversity of landscape genes refers to the various forms of cultural factors in architecture,environment,culture,and layout,including type diversity,combination diversity,and functional diversity.

Application of Landscape Gene Information Chain in Tourism Development of Traditional Human Settlements:A Case Study of Daqitou Village in Sanshui District,Foshan City

Based on the aspect of historical and cultural geography, the value of "landscape gene information chain" in the tourism development of ancient villages was verified by taking cultural landscape as the principal line, historical and cultural settlement as the carriers, and traditional buildings as the entry points. Taking Daqintou Village in Sanshui District, Foshan City for example, the theory of "landscape gene information chain" was applied to design a tourism planning scheme for Daqitou Village.

Theories and Practices of Remembering Nostalgia in New Urbanization

As the National Conference on New Urbanization proposed, "(new urbanization) should enable residents to see mountains and waters, and remember nostalgia". Nostalgia is a kind of memories and yearning deep in the heart for one's homeland or the place once lived in, it is the softest emotion and also a spiritual need. To keep nostalgia in new urbanization is to preserve history, culture, memories, and humanistic spirits of the homeland, and also to maintain local cultural genes and carry forward rural civilization. The foundation of remembering the nostalgia is to intensify culture protection. And by means of protecting cultural heritage and style of the traditional settlement practically, preserving cultural gene, inheriting cultural memory, creating cultural brand and characteristic landscape etc., the nostalgia can meet the spiritual demands of the residents, such as homesickness, life emotion, historical emotion and cultural emotion in the process of new urbanization. The key of remembering nostalgia is to promote the construction of small characteristic towns. Relying on on-site urbanization, the signifi cant ways of achieving new urbanization are star-studded layout of small towns and the construction of small characteristic tourism town. The construction of Hunan-style Tourism Town is a positive attempt and exploration of remembering nostalgia, and it contributes to the construction of a beautiful and poetic homeland for the human-centered new urbanization.

Study on security and protection of original ecotourism resources in the mountain areas of western Guangxi

Original ecotourism resources mainly refer to natural and human original ecotourism resources, and it＇s of fragility, rarity and irreversibility. As a valuable historic heritage and hnportant tourism resources, it plays a significant role in developing tourism and economy in such areas as the underdeveloped areas, the mountain areas and mimority areas. The tourism resources in the western mountain areas of Guangxi owe ,superiorities and characteristics ＇o their original ecology, Yet, western Guangxi is an ethnic region with fragile karst eco-environment, so it is specialto exploit the tourism resources. The paper defines original eeotourism and analyses the specialties and advantages of the original ecotourism resources as well as thefrailty of the ecotourism resources of the region. The ways of thinking are put forward for safe preservation and sustainable development of the original ecotourism resources, i.e. carrying out measures for the multi-grade protection of heritage resources, setting up a ethnic eco-museum/ ethnic culture eco-park and a gene pool of ethnic cultures, etc.

Adherence of uropathogenic Escherichia coli to human primary epithelial cells of renal pelvis

Human primary epithelial cells of renal pelvis was established to investigate the adherence of uropathogenic Escherichia coli （UPEC） to this cell line, in which the primary cell culture was performed by using cultivation of the normal epithelium of renal pelvis in keratinocyte serum free medium （K-SFM） with epidermal growth factor （EGF） and bovine pituitary extract （BPE）. Both UPEC132 obtained from urine specimen of patients with pyelonephritis and the pilus-free representative strain E. coli K-12p678- 54 were used to study the adherence of these strains on human primary epithelial cells of renal pelvis. The UPEC adherence was performed with observation on the morphological changes of the adhered cells, while the adhesion rates and indices were calculated in different times of experiment. In addition, the virulence genes hly and cnfl of UPEC132 were detected by multiplex PCR assay. In this study, the human primary epithelial cells of renal pelvis was found to exhibit the character of the transitional epithelial cells. Compared with the control group, the adhesion rates and indices began to increase from 15 min of the experiment time and reached its peak in 120 min. The adhesion rate and index of UPEC132 to human primary epithelial cells of renal pelvis were 74.4% and 34.0 respectively. Many microscopic changes in the primary cells adhered with UPEC132 could be detected, such as rounding or irregularity in shape, unevenness in staining and the cytoplasmic and nuclear changes. It suggests that human primary epithelial cells of renal pelvis can be used for the experiment on UPEC adhesion, thus providing a basis for the further study on the pathogenesis of UPEC.

The Archaeological Interpretation of the Determining Criteria and Developmental Path of Chinese Civilization

The research findings of the project of tracing the origins of Chinese civilization substantiate China's million years of human history,10,000-plus years of cultural history,and 5,000-plus years of civilizational history.Cities and capitals have been the foremost tangible embodiment throughout the formation and development of Chinese civilization.By examining the sites of ancient Chinese capitals and palaces,noting the transition from“shrines side by side with palaces”to“the imperial ancestral shrine on the left and the altar to the gods of earth and grain on the right,”and observing the evolution from“dual axis”to“central axis”in city planning,we can delve into the cultural genes that have shaped the development of Chinese civilization.Throughout history,China's first inhabitants and their descendants have forged strong traditions of ancestral identification,the unity and commensurability of family and state,and identification with the state.Characterized by powerful cohesiveness and inclusiveness,these traditions form the subtly ingrained and daily practiced cultural genes of Chinese civilization.They are the fundamental reasons behind the enduring continuity of Chinese civilization for over 5,000 years.

Between“Guandai”(冠带)and“Yidi”(夷狄):Sima Qian's National Consciousness

During the reign of Emperor Wu of Han Dynasty,the ethnic relations in China entered a new historical stage.Records of the Historian written during this period gives a basically factual account of the ethnic history in China since ancient epochs.It is the first specialized work on ethnic history.The national consciousness that Sima Qian presents in this work is distinctively different from the Confucian tradition,which draws a strict line between Hua(华,China)and Yi(夷,the barbarians).His attention on the commonality of the cultural genes between the farming peoples of the Central-Plains and the nomadic hunting peoples of the borderlands,his recognition of the integrated impact that multiple ethnic groups have on the rise and development of Huaxia(华夏,Chinese)civilization,his respect for"differences among barbarians of the four directions"(四夷各异),and his approval of the“merits of Hu attire”(胡服之功)are all embodiment of his liberal views.To explore the formation and development of the cultural community of the Chinese nation and to understand and interpret this historical process of ethnic integration,we should attach great importance to the positive significance of Sima Qian's national consciousness.

Production of Active Compounds in Medicinal Plants:From Plant Tissue Culture to Biosynthesis

Over past decades plant tissue culture has emerged as an alternative of whole plant cultivation in the production of valuable secondary metabolites.Adventitious roots culture of Panax ginseng and Echinacea purpure has reached the scale of 1-10 kL.Some molecular biological techniques,such as transgenic technology and genetic stability are increasingly used in the studies on plant tissue cultures.The studies on elicitors have deepened into the induction mechanism,including signal molecules,functional genes,and so on.More and more biological elicitors,such as A.niger and yeast are used to increase the active compounds in plant tissue cultures.We also discussed the application of synthetic biology in the studies on biosynthesis of artemisinin,paclitaxel,and tanshinon.The studies on active ingredients biosynthesis of medicinal plants provide unprecedented possibilities to achieve mass production of active ingredients.Plant tissue cultures can not only produce active ingredients but also as experimental materials for biosynthesis.In order to improve the contents of active compounds in medicinal plants,following aspects could be carried out gene interference or gene silencing,gene overexpression,combination with chemical synthesis,application of elicitors,and site-directed mutagenesis of the key enzymes.

SHORT COMMUNICATIONS Comparison of inositol phosphates formation and gene expression of Gq alpha subunit in cultured aortic smooth muscle cells from spontaneously hypertensive and Wistar Kyoto rats

Abstract Objecitve To explore whether phosphoinositide specific phospholipase C (PLC) activation via G protein in vascular smooth muscle cells (VSMCs) is altered in spontaneously hypertensive rats (SHR). Methods The VSMCs derived from aortae of SHR and Wistar Kyoto (WKY) rats were loaded for 48 hours with myo inositol. Inositol phosphate release was initiated by the addition of 10 5 mol/L norepinephrine in intact cells or by guanosine 5' 0 (3 thio tri sphosphate) (GTP gamma S) in permeabilized cells. In the meantime, growth arrested VSMCs were stimulated by 10% calf serum for 0, 30, 45, or 60 min, then gene expressions of Gq alpha subunit (G alph a q) were observed. Results There were no significant differences in inositol 1, 4,5 triphosphate (IP 3) level and expression of G alpha q mRNA between quiescent VSMCs from SHR and that from WKY. When stimulated by norepinephrine, IP 3 production increased transiently with a peak level at 10 s in VSMCs from WKY, and a rapid biphasic IP 3 response, which was significantly higher than that of WKY, in VSMCs from SHR had been observed. G proteins activated by GTP gamma S significantly raised IP 3 production in VSMCs from SHR compared to WKY (SHR vs WKY: 234.8%±29.2% vs 142.4%±12.0% of basal IP 3, P<0.05). In addition, the serum effect showed an significant increase in expression of G alpha q mRNA in VSMCs from SHR. Conclusions The hereditary factors are not the only variable regulating IP 3 metabolism and G alpha q gene expression. Influences of multi environmental factors such as vasoactive compounds, together with genetic predisposition, palys an important role in the highly sensitive response of IP 3 production and G alpha q gene over expression in SHR.