The Probability Density Function Related to Shallow Cumulus Entrainment Rate and Its Influencing Factors in a Large-Eddy Simulation

The process of entrainment-mixing between cumulus clouds and the ambient air is important for the development of cumulus clouds.Accurately obtaining the entrainment rate(λ)is particularly important for its parameterization within the overall cumulus parameterization scheme.In this study,an improved bulk-plume method is proposed by solving the equations of two conserved variables simultaneously to calculateλof cumulus clouds in a large-eddy simulation.The results demonstrate that the improved bulk-plume method is more reliable than the traditional bulk-plume method,becauseλ,as calculated from the improved method,falls within the range ofλvalues obtained from the traditional method using different conserved variables.The probability density functions ofλfor all data,different times,and different heights can be well-fitted by a log-normal distribution,which supports the assumed stochastic entrainment process in previous studies.Further analysis demonstrate that the relationship betweenλand the vertical velocity is better than other thermodynamic/dynamical properties;thus,the vertical velocity is recommended as the primary influencing factor for the parameterization ofλin the future.The results of this study enhance the theoretical understanding ofλand its influencing factors and shed new light on the development ofλparameterization.

Effects of Cumulus Cells on in vitro Fertilization of Bovine

[Objective] The aim was to explore the effect of cumulus cells on the in vitro fertilization of in vitro matured bovine oocytes. [Method] The in vitro matured oocytes were divided into three groups of cumulus cells removal, partial removal and no removal. [Result] In the co-culture with cumulus cells, the oocytes of the removal group had higher cleavage rate and blastocyst rate （74.4%±4.1, 53.7%±5.1） than those of the no removal group （72.7%±5.1, 52.4%±3.5）, but the difference was not significant （P〉0.05）, while both groups had better performances than the re- moval group （39.6%±4.5, 18.8%±4.6） with the difference reaching the significant level （P〈0.05）. All the three groups showed significant difference with the control. The combination of cumulus cells and melatonin achieved the best effects as the cleavage rate and blastocyst rate of the partial removal group （79.8%±3.7, 56.5%±5.1） were better than those of the no removal group （78.2%±2.6, 55.8%±4.6）, and the difference was not significant, while both group had better performances than the removal group （48.3%±5.5, 22.7%±4.3） and the control group with the differences reaching the significant level （P〈0.05）. [Conclusion] The study provided technical support for the production of dairy cows and beef cattle.

开放平台的价值引导:CUMULUS和DESIS的结盟

本文介绍了CUMULUS和DESIS两个不同性质的国际组织的结盟过程,在国际设计教育联盟多元价值共存基础上倡导了全球设计院校的社会责任,创造了一个双赢的局面。这种合作对推进国际设计教育和设计学科转型和社会的可持续发展将带来深远的影响。

Heat exposure impairs porcine oocyte quality with suppressed actin expression in cumulus cells and disrupted F-actin formation in transzonal projections

Background:Transzonal projections(TZPs)constitute a structural basis for the communication between the oocyte and its surrounding cumulus cells(CCs),which play critical roles in promoting the oocyte maturation.Previously we found that heat stress(HS)causes loss of TZPs in porcine cumulus-oocyte complexes(COCs)with decreased density of filamentous actin(F-actin).However,the time-course responses of F-actin and its monomeric actins(β-actin andγ-actin)during the in vitro maturation of oocytes remain unclear.Results:In this study,excised porcine ovaries were exposed to HS at 41.5°C for 1 h before COCs were isolated and matured in vitro for 44 h.HS significantly reduced oocyte quality,characterized by impaired cumulus expansion,delayed meiotic resumption and lower survival rate and polar body extrusion rate,as well as decreased expression of mitochondrial DNA-encoded genes and elevated mitochondrial reactive oxygen species concentration.Expression ofβ-actin andγ-actin in CCs increased gradually with oocytes maturation,which was significantly reduced in HS group,especially at 24 h and/or 44 h of in vitro maturation.By contrast,the number of TZPs and the fluorescence intensity of F-actin in zona pellucida decreased gradually during oocytes maturation,which were significantly reduced by HS at 24 h of in vitro maturation.Moreover,colocalization analyses revealed bothβ-actin andγ-actin contribute to the F-actin formation in porcine TZPs,and the colocalization of F-actin with GJ protein connexin 45 was significantly reduced in heat-exposed COCs.Conclusions:The results indicate that the suppression of actin expressions in CCs,which may lead to the F-actin unstabilization in TZPs,will subsequently contribute to the compromised quality of oocytes under HS.

ENSEMBLE PREDICTION EXPERIMENTS OF TRACKS OF TROPICAL CYCLONES BY USING MULTIPLE CUMULUS PARAMETERIZATION SCHEMES

Ensemble prediction experiments of the tracks of eight tropical cyclones occurring between 2004-2006 over the western Pacific have been performed by using MM5 with five cumulus parameterization schemes. The results show that the predictions of the tracks of the tropical cyclones are sensitive to the selection of cumulus parameterization schemes. Each scheme has its own advantage and disadvantage, and the predications without cumulus parameterization schemes are not the worst, sometimes even better than the others. And all of the three ensemble methods improve the predictions of the tracks significantly, among which the ensemble method without parameterization schemes, the Grell, Betts-Miller and Kain-Fritsch schemes are the best.

Intensive Radiosonde Measurements of Summertime Convection over the Inner Mongolia Grassland in 2014:Difference between Shallow Cumulus and Other Conditions

Using radiosonde measurements from 26 July to 30 July 2014 at Baiqi over the Inner Mongolia grassland of China, the vertical structure of shallow cumulus （SCu） clouds and associated environmental conditions were investigated. The cloud base height and the cloud top height of SCu was 3.4 km and 5 km, respectively. The temperature of the SCu layer was less than 0℃. The horizontal advection of specific humidity was smaller than the vertical transport in the atmosphere below 5 km. Above 5 km, the thermodynamic structure of the atmosphere remained stable. At the interface of the cloud layer and free air atmosphere, there was obvious wind shear and a temperature inversion （-2.9~C）. Comparisons of environmental parameters associated with cumulus congestus, rain and clear days, showed that the formation of SCu was characterized by a higher Bowen ratio （high sensible heat flux and low latent heat flux）, which indicated intensive turbulence in the boundary layer. The formation of SCu was associated with the boundary layer height exceeding the lifting condensation level. The maintenance of SCu was likely associated with the lower convective available potential energy, weak wind shear, and weak subsidence of the synoptic system, which did not favor the dramatic vertical development of SCu and thereby the transformation of SCu to cumulus congestus.

Impacts of Cumulus Momentum Transport on MJO Simulation

Vertical cumulus momentum transport is an important physical process in the tropical atmosphere and plays a key role in the evolution of the tropical atmospheric system. This paper focuses on the impact of the vertical cumulus momentum transport on Madden-Julian Oscillation （MJO） simulation in two global climate models （GCMs）. The Tiedtke cumulus parameterization scheme is applied to both GCMs [CAM2 and Spectral Atmospheric general circulation Model of LASG/IAP （SAMIL）]. It is found that the MJO simulation ability might be influenced by the vertical cumulus momentum transport through the cumulus parameterization scheme. However, the use of vertical momentum transport in different models provides different results. In order to improve model＇s MJO simulation ability, we must introduce vertical cumulus momentum transport in a more reasonable way into models. Furthermore, the coherence of the parameterization and the underlying model also need to be considered.

Sensitivity Study of the South China Sea Summer Monsoon in 1998 to Different Cumulus Parameterization Schemes

In this study, the improved high-resolution regional climate model of the China National Climate Center （RegCM_NCC） is used to examine the sensitivity of the simulated circulation and rainfall during the South China Sea summer monsoon （SCSSM） period during 1998 in an effort to compare to other cumulus param- eterization schemes. The investigation has indicated that the model is capable of simulating the seasonal march of the SCSSM and that the results were very sensitive to the choice of cumulus parameterization schemes. It seems that the Kuo cumulus parameterization scheme simulates the process of the SCSSM onset reasonably well, which can reproduce the onset timing and dramatic changes before and after the onset, especially the upper- and lower-level wind-fields. However, there are still some discrepancies between the simulations and observations. For example, the model can not completely simulate the intensity of the rainfall or the location of the western Pacific subtropical high as well as the feature of the rapid northward propagation of seasonal rain belt.

Impact of Horizontal Resolution and Cumulus Parameterization Scheme on the Simulation of Heavy Rainfall Events over the Korean Peninsula

In this paper, we present the results from high-resolution numerical simulations of three heavy rainfall events over the Korean Peninsula. The numerical results show that the prediction accuracy for heavy rainfall events improved as horizontal resolution increased. The fine-grid precipitation fields were much closer to the real precipitation fields in the case of large synoptic forcing over the Korean Peninsula. In the case of large convective available potential energy and weak synoptic forcing, it seems that even when using a high resolution, the models still showed poor performance in reproducing the observed high precipitation amounts. However, activation of the cumulus parameterization scheme in the intermediate resolution of 9 km, even at a grid spacing of 3 km, had a positive impact on the simulation of the heavy rainfall event.

Effect of exogenous progesterone on cumulus characteristics of buffalo oocytes by allowing passage of more number of sperm through cumulus but not essentially fertilization

Objective:To understand the level of progesterone (P4) in different quality of buffalo cumulus oocyte complexes (COCs) and further to evaluate the effect of exogenous P4 supplementation on maturation and subsequent developmental ability of poor quality brilliant cresyl blue (BCB-)COCs.Methods: Progesterone secreted by different quality of buffalo oocytes was estimated by enzyme linked immunosorbent assay and the concentration differences were translated into P4 doses to be incorporated in the maturation medium of BCB-ve COCs followed by expression analysis of genes involved in the cumulus expansion, extracellular matrix disintegration and progesterone receptor signalling. In addition, the study also evaluated the effect of exogenous P4 on sperm-cumulus interaction. Results: More than 10-fold up-regulated expression of progesterone receptor in P4 supplemented oocytes signified that P4 might be acting predominantly through this receptor. Also, exogenous P4 supplementation had significant effect on transcatheter arterial chemoembolization protease regulated by P4-progesterone receptor pathway which in turn had an important role in extracellular matrix disintegration. On the contrary, cumulus expansion genesHAS2,TNFAIP6,AREG were not altered upon P4 supplementation. Also, it was observed that P4 addition did facilitate passage of significantly more number of spermatozoa through P4 treated cumulus cells. Further, incorporation of different doses of P4 did not improve significantly the cleavage and blastocyst rates of BCB-ve COCs.Conclusions: Different qualities of buffalo COCs secrete substantially diverse levels of P4, and its supplementation has a role in oocyte maturation via modulation of cumulus characteristics but perhaps not fertilization.

Microphysical characteristics of precipitating cumulus cloud based on airborne Ka-band cloud radar and droplet measurements

Based on cloud-probe data and airborne Ka-band cloud radar data collected in Baoding on 5 August 2018,the microphysical structural characteristics of cumulus(Cu)cloud at the precipitation stage were investigated.The cloud droplets in the Cu cloud were found to be significantly larger than those in stratiform(STF)cloud.In the Cu cloud,most cloud particles were between 7 and 10μm in diameter,while in the STF cloud the majority of cloud particles grew no larger than 2μm.The sensitivity of cloud properties to aerosols varied with height.The cloud droplet effective radius showed a negative relationship with the aerosol number concentration(Na)in the cloud planetary boundary layer(PBL)and upper layer above the PBL.However,the cloud droplet concentration(Nc)varied little with decreased Na in the high liquid water content region above 1500 m.High Na values of between 300 and 1853 cm-3 were found in the PBL,and the maximum Na was sampled near the surface in August in the Hebei region,which was lower than that in autumn and winter.High radar reflectivity corresponded to large FCDP(fast cloud droplet probe)particle concentrations and small aerosol particle concentrations,and vice versa for low radar reflectivity.Strong updrafts in the Cu cloud increased the peak radius and Nc,and broadened cloud droplet spectrum;lower air temperature was favorable for particle condensational growth and produced larger droplets.

Validation for a tropical belt version of WRF: sensitivity tests on radiation and cumulus convection parameterizations

Version 3.9 of WRF-ARW is run with a tropical belt configuration for a period from 2012 to 2016 in this study. The domain covers the entire tropics between 45°S and 45°N with a spatial resolution of about 45 km. In order to verify two radiation schemes and four cumulus convection schemes, eight experiments are performed with different combinations of physics parameterization schemes. The results show that eight experiments present reasonable spatial patterns of surface air temperature and precipitation in boreal summer, with the spatial correlation coefficient (COR) between simulated and observed temperature exceeding 0.95, and that between simulated and observed precipitation ranges from 0.65 to 0.82. The four experiments with the RRTMG radiation scheme show a better performance than the other four experiments with the CAM radiation scheme. In the four experiments with the RRTMG radiation scheme, the COR between simulated and observed surface air temperature is about 0.98, and that between simulated and observed precipitation ranges from 0.76 to 0.82. Comparatively, the two experiments using the new Tiedtke cumulus parameterization scheme can simulate better diurnal variation of precipitation in boreal summer than the other six experiments. In particular, for the diurnal cycle of precipitation over land and ocean, the experiment using the RRTMG radiation scheme and the new Tiedtke cumulus convection scheme shows that the peaks of precipitation rate appear at 0400 LST and 1600 LST, in agreement with observation.

THE IMPACT OF CUMULUS PARAMETERIZATIONS AND MICROPHYSICS SCHEMES UNDER DIFFERENT COMBINATIONS ON TYPHOON TRACK PREDICTION

This study examines the effects of cumulus parameterizations and microphysics schemes on the track forecast of typhoon Nabi using the Weather Research Forecast model. The study found that the effects of cumulus parameterizations on typhoon track forecast were comparatively strong and the typhoon track forecast of Kain-Fritsch (KF) was superior to that of Betts-Miller (BM). When KF was selected, the simulated results would be improved if microphysics schemes were selected than otherwise. The results from Ferrier, WSM6, and Lin were very close to those in the best track. KF performed well with the simulations of the western extension and eastern contraction changes of a North Pacific high as well as the distribution and strength of the typhoon wind field.

Effect of Early Cumulus Cell Removal on the Fertilization and Clinical Outcome in Human <i>in Vitro</i>Fertilization

The short-term insemination technique has been widely applied, although it is not clear whether the mechanical desorption of cumulus cells can affect normal fertilization and birth outcomes. Therefore, the present study aimed to evaluate whether early cumulus cell removal could affect in vitro fertilization outcomes. Based on a history of infertility and semen analysis, 61 patients were identified, and the cumulus cells were mechanically removed at either6 h post-insemination (Group A, 387 mature oocytes) or 18 - 20 h post-insemination as traditional in vitro fertilization (Group B, 424 mature oocytes). There were no significant differences in the rates of fertilization, available embryos, high quality embryos, birth, premature birth, pregnancy, or implantation when we compared the two groups. However, Group A exhibited a significantly higher polyspermy rate, and significantly lower rates of blastocyst formation and high-quality blastocyst formation, compared with Group B. Therefore, the results of present study indicate that early cumulus cell removal (at 6 h post-insemination) may negatively affect the rates of polyspermy, blastocyst formation, and high-quality blastocyst formation.

A MODIFIED CUMULUS PARAMETERIZATION SCHEME AND ITS APPLICATION IN THE SIMULATIONS OF THE HEAVY PRECIPITATION CASES

According to the characteristics of organized cumulus convective precipitation in China,a cumulus parameterization scheme suitable for describing the organized convective precipitation in East Asia is presented and modified.The Kain-Fristch scheme is chosen as the scheme to be modified based on analyses and comparisons of simulated precipitation in East Asia by several commonly-used mesoscale parameterization schemes.A key dynamic parameter to dynamically control the cumulus parameterization is then proposed to improve the Kain-Fristch scheme.Numerical simulations of a typhoon case and a Mei-yu front rainfall case are carried out with the improved scheme,and the results show that the improved version performs better than the original in simulating the track and intensity of the typhoons,as well as the distribution of Mei-yu front precipitation.

A Comparative Study of the Numerical Simulation of the 1998 Summer Flood in China by Two Kinds of Cumulus Convective Parameterized Methods

The NCC T63L20 model of the National Climate Center, China Meteorological Administration is employed to simulate the 1998 summer flood, which mainly occurred in the region of the Yangtze River and Northeast China. For this study, two kinds of cumulus convection parameterized schemes are employed in this model respectively. The simulations show that the Gregory parameterized scheme, which is still used in the United Kingdom Meteorological Office routine model, simulates more reasonable rainfall amount and distribution compared to the Kuo-type scheme. Moreover, the Gregory scheme better simulates the tendency of general circulation than the Kuo-type scheme. On the whole, the Gregory scheme provides a good simulation of the main features of the seasonal precipitation and general circulation in China, although the simulated result still exhibits some departures from the observations.

Sensitivity of Numerical Simulations of the East Asian Summer Monsoon Rainfall and Circulation to Different Cumulus Parameterization Schemes

A 5-level spectral AGCM (ImPKU-SLAGCM) is used to examine the sensitivity of the simulated results of the summer monsoon rainfall and circulation in East Asia to different cumulus parameterization schemes in the climatological-mean case and in the cases of weak and strong Asian summer monsoons, respectively. The results simulated with the Arakawa-Schubert's(hereafter A-S's), Kuo's and Manabe's cumulus parameterization schemes show that these simulated distributions of the summer monsoon rainfall and circulation in East Asia depend strongly on the cumulus parameterization schemes either in the climatological-mean case or in the cases of weak and strong Asian summer monsoons. From the simulated results, it might be shown that the Kuo scheme appears to be more suitable for the simulation of the summer monsoon rainfall and circulation in East Asia than the A-S scheme or the Manabe scheme, although the A-S scheme is somewhat better in the simulations of the tropical rainfall. This might be due to that the Kuo's cumulus parameterization scheme is able to reflect well the characteristics of rainfall cloud system in the East Asian summer monsoon region, where the rainfall system used to be a mixing of cumulus and stratus.

Evaluation of a Lagrangian advection scheme for cloud droplet diffusion growth with a maritime shallow cumulus cloud case

A Lagrangian advection scheme(LAS)for solving cloud drop diffusion growth was previously proposed(in 2020)and validated with simulations of cloud droplet spectra with a one-and-a-half dimensional(1.5D)cloud bin model for a deep convection case.The simulation results were improved with the new scheme over the original Eulerian scheme.In the present study,the authors simulated rain embryo formation with the LAS for a maritime shallow cumulus cloud case from the RICO(Rain in Cumulus over the Ocean)campaign.The model used to simulate the case was the same 1.5D cloud bin model coupled with the LAS.Comparing the model simulation results with aircraft observation data,the authors conclude that both the general microphysical properties and the detailed cloud droplet spectra are well captured.The LAS is robust and reliable for the simulation of rain embryo formation.

Some Characteristics of Cumulus Convection over the Tibetan Plateau

The diagnostic model of the cumulus convection proposed by Yanai et al. (1973) was applied to the atmosphere over the Tibetan Plateau, and used to estimate the vertical mass flux, entrainment and detrainment, excess temperature and moisture, liquid water content, and condensation and precipitation rates of highland cloud clusters. The results illustrated that in clouds over the Tibetan Plateau, the water vapor condensation rate, liquid water content, and efficiency of the rain generation process are less than those in the tropics (represented by the Marshall Islands region). Therefore, the condensational latent heat released over the Tibetan Plateau, overall, is much smaller than that in the tropics. The water vapor and liquid water detrainment from shallow nonprecipitating cumulus clouds, and their entrainment into deep cumulus clouds, serve as a growing mechanism for the deep precipitating cumulus towers over the Tibetan Plateau. It should be noted that there is a stronger detrainment of liquid water from cumulus clouds and a stronger re-evaporation rate in environment. The process of the condensation-detrainment-re-evaporation-cnlrainmcnt is repeatedly in progress. It would play an important role in maintaining of cumulus convection on the condition that the supply of moisture is not plentiful over the Tibetan Plateau.The analyses also showed that the cloud mass flux Mc over the Tibetan Plateau is less, and the large-scale average upward motion is much less than those over the Marshall Islands. Stronger compensating downward motion in the cloud environment over the Tibetan Plateau, responsible for the area's strong environmental heating rate Was revealed, and would link to the stability of the South Asian High in summer.

Roles of trifluoperazine and verapamil in the oocyte maturation and cumulus expansion of bovine cumulus－oocyte complexes

Bovine cumulus-oocyte complexes were cultured in the maturation medium containing 4 different concentrations of verapamil and trifluoperazine to testify the necessity of extracellular Ca2+ and Ca2+-calmodulin complex for the resumption and completion of meiosis as well as cumulus expansion.Ultrastructure of the treated oocytes was also observed to investigate the cytoplasm maturation. The results showed that verapamil didn't influence the cumulus expansion,meiosis resumption and completion and cytoplasm maturation significantly.TFP inhibited cumulus expansion in a dose-dependent manner.25 μm trifluoperazine significantly inhibited the GVBD and maturation(p<0.01),wherease 1 μm TFP had no effect.Both oocytes and cumulus cells treated with 25 μm TFP severely degenerated.Our observations suggest that the resumption and completion of meiosis and cumulus expansion are Ca2+ -CaM dependent and blocking membrane Ca2+ channel does not influence oocyte germinal vesicle breakdown,nuclear and cytoplasm maturation significantly in cattle.