A new macrolide glycoside, cuneataside F was isolated from the n-butanol extract of the stem of Sargentodoxa cuneata. The structure was elucidated on the basis of extensive 1D and 2D NMR as well as HRESI-MS spectrosco...A new macrolide glycoside, cuneataside F was isolated from the n-butanol extract of the stem of Sargentodoxa cuneata. The structure was elucidated on the basis of extensive 1D and 2D NMR as well as HRESI-MS spectroscopic analysis.展开更多
Two new phenylpropanoid glycosides named cuneataside E(1) and cuneataside F(2), were isolated from the aerial parts of Lespedeza cuneata(Dum. Cours.) G. Don, whose structures were E and Z isomer, respectively. Their s...Two new phenylpropanoid glycosides named cuneataside E(1) and cuneataside F(2), were isolated from the aerial parts of Lespedeza cuneata(Dum. Cours.) G. Don, whose structures were E and Z isomer, respectively. Their structures were elucidated on the basis of comprehensive spectroscopic analysis(UV, IR, HR-ESI-MS, 1D and 2D NMR). In in vitro bioassays at 10 μmol/L, compound 1showed moderate hepatoprotective activity against N-acetyl-p-aminophenol(APAP)-induced toxicity in He G2 cells.展开更多
[目的]采用数据挖掘和网络药理学初步探讨大血藤治疗高脂血症的可能作用机制。[方法]借助PubMed和CNKI检索“大血藤化学成分”或“红藤成分”进行资料收集和化合物筛选,用Origin Pro 2021软件对涉及中药的功效类别等进行频数分析,用SPSS...[目的]采用数据挖掘和网络药理学初步探讨大血藤治疗高脂血症的可能作用机制。[方法]借助PubMed和CNKI检索“大血藤化学成分”或“红藤成分”进行资料收集和化合物筛选,用Origin Pro 2021软件对涉及中药的功效类别等进行频数分析,用SPSS Modeler 18.0软件进行关联规则分析和聚类分析。利用Swiss ADME平台来预测候选化合物的吸收度和类药性的相关参数。采用Swiss Target Prediction平台来收集预测到的所有靶点,以“hyperlipidemia”“dyslipidemia”等为关键词检索Genegards、Drug Bank Database等数据库中高脂血症的潜在靶点,通过Uniprot数据库去重和校正靶点名称,取活性成分和高脂血症靶点交集,进而运用Cytoscape 3.8.0软件来进行网络可视化并筛选核心靶点基因。通过DAVID 6.8数据库进行基因本体(GO)功能富集分析和京都基因和基因组百科全书(KEGG)通路富集分析,预测交集靶点作用机制,并绘制气泡图等进行可视化。[结果]通过数据挖掘对大血藤的用药配伍规律进行分析,结果显示性味以温性、寒性和苦味、辛味为主,归经方面以肝、脾为主,配伍以当归、甘草、红花等关联密切,研究结果与中药治疗高脂血症用药规律一致。中药-化合物-靶点网络包含了43个活性成分和相应靶点133个,关键靶点涉及类视黄酸受体α(RXRA)、信号转导及转录激活蛋白3(STAT3)、90 kDa热休克蛋白αA1(HSP90AA1)、蛋白激酶Bα(AKT1)、磷酸肌醇3-激酶调节亚基1(PIK3R1)、雌激素受体1(ESR1)、过氧化物酶体增殖物激活受体(PPAR)A、核受体亚家族3C组成员1(NR3C1)等。GO功能富集分析得到条目437个(P<0.01),KEGG通路富集分析筛选得到57条信号通路(P<0.05)。[结论]大血藤可以通过多个活性成分干预血脂异常中的多个靶点、多个环节,从多维、系统的层面参与调控血脂代谢和高脂血症的发生发展过程,为大血藤的进一步开发利用提供理论基础。展开更多
目的:了解3个不同产地、2个不同批次的红藤饮片6种次生代谢产物含量、抑菌活性及两者的相关性。方法:利用分光光度法对红藤饮片提取物的6种次生代谢产物含量进行分析,以金黄色葡萄球菌和枯草芽孢杆菌为实验菌株,用纸片琼脂扩散法测定抑...目的:了解3个不同产地、2个不同批次的红藤饮片6种次生代谢产物含量、抑菌活性及两者的相关性。方法:利用分光光度法对红藤饮片提取物的6种次生代谢产物含量进行分析,以金黄色葡萄球菌和枯草芽孢杆菌为实验菌株,用纸片琼脂扩散法测定抑菌圈大小;以平板二倍稀释法统计最小抑菌浓度(M IC);以试管二倍稀释法统计最小杀菌浓度(M BC)。结果:红藤药液对金黄色葡萄球菌和枯草芽孢杆菌的抑菌活性以安徽产地第一批次最高,抑菌圈大小、M IC、M BC分别为(9.67±0.29)mm、12.5 m g/m l、31.25 m g/m l和(10.17±0.58)mm、12.5 m g/m l、31.25 m g/m l,江苏产地第二批次最低,抑菌圈大小、M IC、M BC分别为(7.67±0.29)mm、25 m g/m l、125 m g/m l和(8.17±0.29)mm、25 m g/m l、125 m g/m l。红藤饮片提取物对金黄色葡萄球菌的抑菌活性在不同产地和不同批次之间均有显著性差异(P<0.01);对枯草芽孢杆菌的抑菌活性在不同产地亦有显著性差异(P<0.01),但不同批次之间无显著性差异(P>0.05)。红藤药液的6种次生代谢产物总量以安徽产地第一批次最高(3.61%),江苏产地第二批次最低(1.90%)。红藤提取物的总生物碱和游离蒽醌在不同产地之间有显著性差异(P<0.01),而在不同批次之间无显著性差异(P>0.05);总绿原酸、黄酮类化合物、总鞣质和总皂苷在不同产地和不同批次之间均有显著性差异(P<0.01)。影响红藤饮片对2种细菌的抑菌活性的主要次生代谢产物可能为总皂甙、总鞣质、游离蒽醌及总绿原酸。结论:不同红藤饮片的次代谢产物的含量及抑菌活性以安徽和浙江产地较高,江苏产地较低;红藤的抑菌活性与饮片中总皂甙、总鞣质、游离蒽醌及总绿原酸的含量密切相关。展开更多
Several methods were used to extract the flavonoids from the leaves of Sargentodoxa cuneata while concentration of flavonoids in the extraction was determined by different methods. The suitable quantitative methods us...Several methods were used to extract the flavonoids from the leaves of Sargentodoxa cuneata while concentration of flavonoids in the extraction was determined by different methods. The suitable quantitative methods used in the ecological analysis of flavonids was that using ultraviolet and 70% ethanol was the best reagent for extraction. Heating was not necessary for extraction. The content of flavonoids from different organs was leafblade, annual twig, leafstalk and perennial stem in turn.展开更多
The contents of flavonoid in the leaf and stem of Sargentodoxa cuneata (Oliv.) Rehd. et Wils. were determined. In a whole growth season, the dynamic chang of flavonoid in the leafblade of S. cuneata showed the two-pea...The contents of flavonoid in the leaf and stem of Sargentodoxa cuneata (Oliv.) Rehd. et Wils. were determined. In a whole growth season, the dynamic chang of flavonoid in the leafblade of S. cuneata showed the two-peak pattern. The first peak appeared at June and the second at September. The seasonal dynamic change of flavonoids in leafstalk, annual twig and perennial stem were different and inconspicuous. The flavonoid cumulated maximally in the leafblade and the followed was seccessively annual twig, leafstalk, and perennial stem. The flavonoid content in leafblade was 31.79 mg/g(DW), while that in annual twig, leafstalk and perennial stem was 5.41 mg/g(DW), 5.30 mg/g(DW) and 3.95 mg/g(DW) respectively. There was markedly higher flavonoid content in leafblade. It is considered that the leaf of Sargentodoxa cuneata is significant in further exploitation.展开更多
文摘A new macrolide glycoside, cuneataside F was isolated from the n-butanol extract of the stem of Sargentodoxa cuneata. The structure was elucidated on the basis of extensive 1D and 2D NMR as well as HRESI-MS spectroscopic analysis.
基金financially supported by the National Mega-project for Innovative Drugs(No.2012ZX09301002-002)National Natural Science Foundation of China(Nos.81560632 and 81202546)
文摘Two new phenylpropanoid glycosides named cuneataside E(1) and cuneataside F(2), were isolated from the aerial parts of Lespedeza cuneata(Dum. Cours.) G. Don, whose structures were E and Z isomer, respectively. Their structures were elucidated on the basis of comprehensive spectroscopic analysis(UV, IR, HR-ESI-MS, 1D and 2D NMR). In in vitro bioassays at 10 μmol/L, compound 1showed moderate hepatoprotective activity against N-acetyl-p-aminophenol(APAP)-induced toxicity in He G2 cells.
文摘目的:了解3个不同产地、2个不同批次的红藤饮片6种次生代谢产物含量、抑菌活性及两者的相关性。方法:利用分光光度法对红藤饮片提取物的6种次生代谢产物含量进行分析,以金黄色葡萄球菌和枯草芽孢杆菌为实验菌株,用纸片琼脂扩散法测定抑菌圈大小;以平板二倍稀释法统计最小抑菌浓度(M IC);以试管二倍稀释法统计最小杀菌浓度(M BC)。结果:红藤药液对金黄色葡萄球菌和枯草芽孢杆菌的抑菌活性以安徽产地第一批次最高,抑菌圈大小、M IC、M BC分别为(9.67±0.29)mm、12.5 m g/m l、31.25 m g/m l和(10.17±0.58)mm、12.5 m g/m l、31.25 m g/m l,江苏产地第二批次最低,抑菌圈大小、M IC、M BC分别为(7.67±0.29)mm、25 m g/m l、125 m g/m l和(8.17±0.29)mm、25 m g/m l、125 m g/m l。红藤饮片提取物对金黄色葡萄球菌的抑菌活性在不同产地和不同批次之间均有显著性差异(P<0.01);对枯草芽孢杆菌的抑菌活性在不同产地亦有显著性差异(P<0.01),但不同批次之间无显著性差异(P>0.05)。红藤药液的6种次生代谢产物总量以安徽产地第一批次最高(3.61%),江苏产地第二批次最低(1.90%)。红藤提取物的总生物碱和游离蒽醌在不同产地之间有显著性差异(P<0.01),而在不同批次之间无显著性差异(P>0.05);总绿原酸、黄酮类化合物、总鞣质和总皂苷在不同产地和不同批次之间均有显著性差异(P<0.01)。影响红藤饮片对2种细菌的抑菌活性的主要次生代谢产物可能为总皂甙、总鞣质、游离蒽醌及总绿原酸。结论:不同红藤饮片的次代谢产物的含量及抑菌活性以安徽和浙江产地较高,江苏产地较低;红藤的抑菌活性与饮片中总皂甙、总鞣质、游离蒽醌及总绿原酸的含量密切相关。
文摘Several methods were used to extract the flavonoids from the leaves of Sargentodoxa cuneata while concentration of flavonoids in the extraction was determined by different methods. The suitable quantitative methods used in the ecological analysis of flavonids was that using ultraviolet and 70% ethanol was the best reagent for extraction. Heating was not necessary for extraction. The content of flavonoids from different organs was leafblade, annual twig, leafstalk and perennial stem in turn.
文摘The contents of flavonoid in the leaf and stem of Sargentodoxa cuneata (Oliv.) Rehd. et Wils. were determined. In a whole growth season, the dynamic chang of flavonoid in the leafblade of S. cuneata showed the two-peak pattern. The first peak appeared at June and the second at September. The seasonal dynamic change of flavonoids in leafstalk, annual twig and perennial stem were different and inconspicuous. The flavonoid cumulated maximally in the leafblade and the followed was seccessively annual twig, leafstalk, and perennial stem. The flavonoid content in leafblade was 31.79 mg/g(DW), while that in annual twig, leafstalk and perennial stem was 5.41 mg/g(DW), 5.30 mg/g(DW) and 3.95 mg/g(DW) respectively. There was markedly higher flavonoid content in leafblade. It is considered that the leaf of Sargentodoxa cuneata is significant in further exploitation.
