AIM To reveal the inhibitory effects ofCurcuma aromatica oil(CAO)on cellproliferation of hepatoma in mice.METHODS Two tumor inhibitory experimentsof CAO on hepatoma in mice were conducted.The inhibitory effects of CAO...AIM To reveal the inhibitory effects ofCurcuma aromatica oil(CAO)on cellproliferation of hepatoma in mice.METHODS Two tumor inhibitory experimentsof CAO on hepatoma in mice were conducted.The inhibitory effects of CAO on proliferation ofhepatoma in mice were evaluated by DNA imagecytometry and immunohistochemical staining ofproliferating cell nuclear antigen(PCNA).RESULTS The tumor inhibitory rates of CAOwere 52% and 51% in two experiments,respectively.Compared with those of the saline-treated control groups,both differences werestatistically significant(P【0.01).In the groupof mice treated with CAO,the cellular nuclearDNA OD value(249±70),areas(623 μm^2±228 μm^2)and DNA(2.38±0.67)index of hepaticcarcinomas were significantly lower than thoseof the control group(430±160,1073 μm^2±101 μm^2 and 4.48±0.71).CAO also couldincrease diploidy cell rates(29.00%±9.34% vs2.97%±5.69%,P【0.01)and decreasepentaploidy cell exceeding rate(30.04%±15.10% vs 70.89%±14.94%,P【0.01).In thegroup of mice treated with CAO,the labelingindexes of proliferating cell nuclear antigen (PCNA-LI)were 30%±4%,which weresignificantly lower than 40%±6% of the controlgroup(P【0.01).CONCLUSION The inhibition of CAO on thegrowth of hepatoma in mice might be associatedwith its depression on cellular proliferativeactivity.展开更多
Objective:To analyze the chemical composition of the essential oils of Curcuma aeruginosa (C.aeruginosa),Curcuma mangga(C.mangga),and Zingiber cassumunar(Z.cassumunar). and study their antimicrobial activity.Methods:E...Objective:To analyze the chemical composition of the essential oils of Curcuma aeruginosa (C.aeruginosa),Curcuma mangga(C.mangga),and Zingiber cassumunar(Z.cassumunar). and study their antimicrobial activity.Methods:Essential oils obtained by steam distillation were analyzed by gas chromatography-mass speclrometry(GC-MS).The antimicrobial activity of the essential oils was evaluated against four bacteria:Bacillus cereus(H.cereus).Staphylococcus aureus(S.aureus).Escherichia coli(E.coli).and Pseudomonas aeruginosa(P.aeruginosa);and two fungi:Candida albicans(C.albicans) and Cyptococcus neoformans(C.neoformans),using disc-diffusion and broth microdilution methods.Results:Cycloisolongifolene,8.9-dehydro-9- formyl(35.29%) and dihydrocoslunolide(22.51%) were the major compounds in C.aeruginosa oil; whereas caryophyllene oxide(18.71%) and caryophyllene(12.69%) were the major compounds in C.mangga oil:and 2,6.9,9-tetramethyl-2.6.10-cycloundecatrien-1-one(60.77%) andα-caryophyllene(23.92%) were abundant in Z.cassumunar oil.The essential oils displayed varying degrees of antimicrobial activity against all lested microorganisms.C.mangga oil had the highest and most broad-spectrum activity by inhibiting all microorganisms tested,with C.neoformans being the most sensitive microorganism by having the lowest minimum inhibitory concentration(MIC) and minimum fungicidal concentration(MFC) values of 0.1μL/mL. C.aeruginosa oil showed mild antimicrobial activity,whereas Z.cassumunar had very low or weak activity against the tested microorganisms.Conclusions:The preliminary results suggest promising antimicrobial properties of C.mangga and C.aeruginosa,which may be useful for food preservation,pharmaceutical treatment and natural therapies.展开更多
Objective: To investigate chemical constituents and new antimicrobial agents among essential oils from the rhizomes of Curcuma aeruginosa(C. aeruginosa) Roxb., Curcuma glans K. Larsen & J. Mood and Curcuma cf. xan...Objective: To investigate chemical constituents and new antimicrobial agents among essential oils from the rhizomes of Curcuma aeruginosa(C. aeruginosa) Roxb., Curcuma glans K. Larsen & J. Mood and Curcuma cf. xanthorrhiza Roxb.Methods: The essential oils were obtained by hydro-distillation and analyzed by gas chromatography/mass spectroscopy. Agar-well diffusion assay was used to study the antimicrobial activity and also broth-micro dilution techniques were examined for minimum inhibitory concentration(MIC) against four bacterial strains and yeast.Results: The gas chromatography/mass spectroscopy analysis showed monoterpenes predominantly(88.53%) in the rhizome oil of Curcuma cf. xanthorrhiza. Sesquiterpenes(50.10%) was the most abundant component in the essential oil of C. glans, while monoterpenes(45.55%) and sesquiterpenes(45.81%) were found in C. aeruginosa with a significant amount. The major components of C. aeruginosa were characterized as camphor(29.39%) and germacrone(21.21%). Germacrone(15.76%), b-pinene(9.97%)and camphor(9.96%) were found as major compounds in the rhizome oils of C. glans while a-terpinolene(24.86%) and p-cymen-7-ol(12.17%) were found as major compositions in Curcuma cf. xanthorrhiza. The essential oils were tested against four bacterial strains and yeast. As a result, the rhizome oil of C. aeruginosa exhibited potent activity against Staphylococcus aureus [inhibition zone(21.94 ± 0.24) mm, MIC 125 μg/mL],Bacillus cereus [inhibition zone(20.83 ± 0.36) mm, MIC 125 μg/mL], and Candida albicans [inhibition zone(11.60 ± 0.30) mm, MIC 250 μg/mL].Conclusions: The essential oils from three Curcuma species possessed greater activity against the gram-positive bacteria(Staphylococcus aureus and Bacillus cereus) than gram-negative bacteria(Escherichia coli and Pseudomonas aeruginosa). The results suggest that the essential oils from the fresh rhizome of Curcuma spp. might be a potential source of natural antimicrobial substances.展开更多
基金National key project of the 9~(th) 5-year Plan for Medicine and Health,No.96-906-07-04Guangdong provincial natural scientific grants,No.980663.
文摘AIM To reveal the inhibitory effects ofCurcuma aromatica oil(CAO)on cellproliferation of hepatoma in mice.METHODS Two tumor inhibitory experimentsof CAO on hepatoma in mice were conducted.The inhibitory effects of CAO on proliferation ofhepatoma in mice were evaluated by DNA imagecytometry and immunohistochemical staining ofproliferating cell nuclear antigen(PCNA).RESULTS The tumor inhibitory rates of CAOwere 52% and 51% in two experiments,respectively.Compared with those of the saline-treated control groups,both differences werestatistically significant(P【0.01).In the groupof mice treated with CAO,the cellular nuclearDNA OD value(249±70),areas(623 μm^2±228 μm^2)and DNA(2.38±0.67)index of hepaticcarcinomas were significantly lower than thoseof the control group(430±160,1073 μm^2±101 μm^2 and 4.48±0.71).CAO also couldincrease diploidy cell rates(29.00%±9.34% vs2.97%±5.69%,P【0.01)and decreasepentaploidy cell exceeding rate(30.04%±15.10% vs 70.89%±14.94%,P【0.01).In thegroup of mice treated with CAO,the labelingindexes of proliferating cell nuclear antigen (PCNA-LI)were 30%±4%,which weresignificantly lower than 40%±6% of the controlgroup(P【0.01).CONCLUSION The inhibition of CAO on thegrowth of hepatoma in mice might be associatedwith its depression on cellular proliferativeactivity.
基金the Kulliyyah of Science,International Islamic University Malaysia for providing financial support and research facilities to accomplish this study,and Dr.Shamsul Khamis of Universiti Putra Malaysia for plant identification
文摘Objective:To analyze the chemical composition of the essential oils of Curcuma aeruginosa (C.aeruginosa),Curcuma mangga(C.mangga),and Zingiber cassumunar(Z.cassumunar). and study their antimicrobial activity.Methods:Essential oils obtained by steam distillation were analyzed by gas chromatography-mass speclrometry(GC-MS).The antimicrobial activity of the essential oils was evaluated against four bacteria:Bacillus cereus(H.cereus).Staphylococcus aureus(S.aureus).Escherichia coli(E.coli).and Pseudomonas aeruginosa(P.aeruginosa);and two fungi:Candida albicans(C.albicans) and Cyptococcus neoformans(C.neoformans),using disc-diffusion and broth microdilution methods.Results:Cycloisolongifolene,8.9-dehydro-9- formyl(35.29%) and dihydrocoslunolide(22.51%) were the major compounds in C.aeruginosa oil; whereas caryophyllene oxide(18.71%) and caryophyllene(12.69%) were the major compounds in C.mangga oil:and 2,6.9,9-tetramethyl-2.6.10-cycloundecatrien-1-one(60.77%) andα-caryophyllene(23.92%) were abundant in Z.cassumunar oil.The essential oils displayed varying degrees of antimicrobial activity against all lested microorganisms.C.mangga oil had the highest and most broad-spectrum activity by inhibiting all microorganisms tested,with C.neoformans being the most sensitive microorganism by having the lowest minimum inhibitory concentration(MIC) and minimum fungicidal concentration(MFC) values of 0.1μL/mL. C.aeruginosa oil showed mild antimicrobial activity,whereas Z.cassumunar had very low or weak activity against the tested microorganisms.Conclusions:The preliminary results suggest promising antimicrobial properties of C.mangga and C.aeruginosa,which may be useful for food preservation,pharmaceutical treatment and natural therapies.
基金supported by the grants from the Biodiversity-Based Economy Development Office Public Organization -National Research Council of Thailand(Grant number.R000012298)Department of Pharmaceutical Sciences,the Faculty of Pharmacy and the Graduate School,Chiang Mai University,Chiang Mai,Thailand
文摘Objective: To investigate chemical constituents and new antimicrobial agents among essential oils from the rhizomes of Curcuma aeruginosa(C. aeruginosa) Roxb., Curcuma glans K. Larsen & J. Mood and Curcuma cf. xanthorrhiza Roxb.Methods: The essential oils were obtained by hydro-distillation and analyzed by gas chromatography/mass spectroscopy. Agar-well diffusion assay was used to study the antimicrobial activity and also broth-micro dilution techniques were examined for minimum inhibitory concentration(MIC) against four bacterial strains and yeast.Results: The gas chromatography/mass spectroscopy analysis showed monoterpenes predominantly(88.53%) in the rhizome oil of Curcuma cf. xanthorrhiza. Sesquiterpenes(50.10%) was the most abundant component in the essential oil of C. glans, while monoterpenes(45.55%) and sesquiterpenes(45.81%) were found in C. aeruginosa with a significant amount. The major components of C. aeruginosa were characterized as camphor(29.39%) and germacrone(21.21%). Germacrone(15.76%), b-pinene(9.97%)and camphor(9.96%) were found as major compounds in the rhizome oils of C. glans while a-terpinolene(24.86%) and p-cymen-7-ol(12.17%) were found as major compositions in Curcuma cf. xanthorrhiza. The essential oils were tested against four bacterial strains and yeast. As a result, the rhizome oil of C. aeruginosa exhibited potent activity against Staphylococcus aureus [inhibition zone(21.94 ± 0.24) mm, MIC 125 μg/mL],Bacillus cereus [inhibition zone(20.83 ± 0.36) mm, MIC 125 μg/mL], and Candida albicans [inhibition zone(11.60 ± 0.30) mm, MIC 250 μg/mL].Conclusions: The essential oils from three Curcuma species possessed greater activity against the gram-positive bacteria(Staphylococcus aureus and Bacillus cereus) than gram-negative bacteria(Escherichia coli and Pseudomonas aeruginosa). The results suggest that the essential oils from the fresh rhizome of Curcuma spp. might be a potential source of natural antimicrobial substances.
基金the Science and Technology Program of Health Department of Zhejiang Province,China(No.2006B107)Science and Technology Program of Wenzhou Municipality,China(No.Y20060085)
