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Cryptococcus curvatusO3酵母菌培养及产油脂特性 被引量:9
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作者 张杰 张晓东 +5 位作者 许海朋 李岩 赵保峰 陈雷 赵宗保 孙立 《微生物学通报》 CAS CSCD 北大核心 2009年第1期41-45,共5页
生物柴油的发展,导致全球油脂供求紧张。微生物油脂的甘三酯组成与植物油类似,发展微生物油脂可部分缓解植物油脂供应压力。本文研究了Cryptococcus curvatusO3酵母利用葡萄糖为碳源生长和积累油脂的特性。Cryptococcus curvatusO3酵母... 生物柴油的发展,导致全球油脂供求紧张。微生物油脂的甘三酯组成与植物油类似,发展微生物油脂可部分缓解植物油脂供应压力。本文研究了Cryptococcus curvatusO3酵母利用葡萄糖为碳源生长和积累油脂的特性。Cryptococcus curvatusO3酵母在培养过程中能适应间歇式碳源流加方式达到高密度培养的目的,但在相同培养条件下,不同氮源能影响其代谢过程中糖到油脂转化的脂肪系数。Cryptococcus curvatusO3酵母利用葡萄糖作为碳源在30°C下摇瓶发酵,菌体生物量为51.8g/L,油脂含量达65.1%。脂肪酸组成分析结果表明,菌油富含饱和及低度不饱和长链脂肪酸,其中饱和脂肪酸之和占总脂肪酸组成的64%左右,其脂肪酸组成类似于可可脂,这些结果对于利用产油微生物转化生物质获取如类可可脂等具有高附加值油脂的研究具有重要意义。 展开更多
关键词 CRYPTOCOCCUS curvatus O3酵母 发酵培养 微生物油脂 脂肪酸
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多纤维素酶协同降解玉米秸秆及水解液微生物油脂发酵研究 被引量:5
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作者 张杰 张晓东 +2 位作者 李岩 许海朋 孙立 《现代化工》 CAS CSCD 北大核心 2008年第S2期133-135,共3页
以富含纤维二糖酶的黑曲霉酶曲协同降解纤维素水解液,利用油脂微生物转化纤维素水解液生产微生物油脂。研究结果表明,酶曲中的纤维二糖酶可以将纤维素水解液中存在的纤维二糖进一步水解成葡萄糖,而油脂酵母Cryptococcus curvatus O3又... 以富含纤维二糖酶的黑曲霉酶曲协同降解纤维素水解液,利用油脂微生物转化纤维素水解液生产微生物油脂。研究结果表明,酶曲中的纤维二糖酶可以将纤维素水解液中存在的纤维二糖进一步水解成葡萄糖,而油脂酵母Cryptococcus curvatus O3又能将葡萄糖迅速转化成微生物油脂储存在体内。Cryptococcus curvatus O3利用纤维素水解液(总还原糖质量浓度为31.5 g/L),经过4次补糖,细胞生物量为49.3 g/L,油脂含量达到60.1%质量分数。 展开更多
关键词 纤维素 酶水解 纤维二糖酶 CRYPTOCOCCUS curvatus O3酵母 微生物油脂
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RIGIDITY THEOREMS OF CLIFFORD TORUS
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作者 张运涛 《Acta Mathematica Scientia》 SCIE CSCD 2010年第3期890-896,共7页
In this article, we prove that the Clifford torus S^1(√1-r^2)×^n-1(r) is the only closed hypersurface in the unit sphere S^n+1(1) with infinite fundamental group, which satisfy r^2≥(n-1)/n,RicM≤C_(H... In this article, we prove that the Clifford torus S^1(√1-r^2)×^n-1(r) is the only closed hypersurface in the unit sphere S^n+1(1) with infinite fundamental group, which satisfy r^2≥(n-1)/n,RicM≤C_(H),and S≤S+(H).Moreover, we give a characterization of Clifford torus S^1(√1-r^2)×^n-1(r) with r^2=2n(1+H^2)^-2(n-1)+nH^2+|H|√n^2H^2+4(n-1). 展开更多
关键词 Principle curvatual Ricci curvature Clifford torus
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Bioleaching of trace elements and rare earth elements from coal fly ash 被引量:7
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作者 Stephen Park Yanna Liang 《International Journal of Coal Science & Technology》 EI 2019年第1期74-83,共10页
Coal fly ash originated from coal combustion has high concentrations of metals. If suitable leaching techniques are identified, then coal fly ash could serve as a useful source of valuable minerals including rare eart... Coal fly ash originated from coal combustion has high concentrations of metals. If suitable leaching techniques are identified, then coal fly ash could serve as a useful source of valuable minerals including rare earth elements (REEs). In this study, three microbial strains, Candida bombicola, Phanerochaete chrysosporium and Cryptococcus curvatus were tested on their performance of leaching trace elements and REEs from fly ash. Through comparing mineral loss and leaching efficiencies resulting from indirect leaching or use of the culture supernatant, C. bombicola was identified to be the best leading to the highest mineral loss and extracting efficiencies of trace elements and REEs among the three strains. The highest mineral loss observed from using the supernatant of this yeast strain was 59.7%. Among all trace elements, As and Mo had the highest leaching efficiency of 80.9% and 79.5%. respectively. The same leaching test led to 67.7% of Yb and 64.6% of Er dissolved from the ash. This study, thus, demonstrated that bioleaching is feasible for leaching metals out of fly ash. The C. bombicola strain deserves further investigation due to its robust actions on metal leaching. 展开更多
关键词 CRYPTOCOCCUS curvatus CANDIDA bombicola PHANEROCHAETE CHRYSOSPORIUM - Coal fly ash BIOLEACHING Rare earth elements Trace metals
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