Characterization and antimicrobial,antioxidant,and anti-proliferative activities of green synthesized magnesium oxide nanoparticles with shoot extracts of Plicosepalus curviflorus

Objective:To synthesize magnesium oxide nanoparticles using ethanol extract of shoots of Plicosepalus curviflorus(PC-MgONPs)and evaluate the antimicrobial,antioxidant,and anti-proliferative activities of PC-MgONPs.Methods:The green synthesized PC-MgONPs were characterized by ultraviolet-visible(UV),Fourier-transform infrared spectroscopy,zeta potential,energy dispersive X-ray,and scanning electron microscopy.Furthermore,we investigated total antioxidant capacity and antimicrobial and anti-proliferative activities using breast cancer cell lines(MDA-231).Results:The UV spectrum of PC-MgONPs showed a sharp absorption peak at 300 nm.The presence of magnesium,oxygen,and sodium was confirmed by energy dispersive X-ray analysis.Scanning electron microscopy revealed PC-MgONPs as roughly spherical granular structures with sizes ranging from 20.0 to 76.4nm.PC-MgONPs showed considerable antimicrobial activities against Escherichia coli,Staphylococcus aureus,methicillin-resistant Staphylococcus aureus,Pseudomonas aeruginosa and Candida albicans with zones of inhibition of 11-17 mm.In addition,total antioxidant capacity and anti-proliferative activity of PC-MgONPs against MDA-231 cells were dose-dependent.Conclusions:The synthesized PC-MgONPs could be a potent antimicrobial,antioxidant and anti-cancer agent,which needs further investigation.

Antimalarial and neuroprotective ent-abietane diterpenoids from the aerial parts of Phlogacanthus curviflorus

Six new ent-abietane diterpenoids,abientaphlogatones A−F(1−6),along with two undescribed ent-abietane diterpenoid glucosides,abientaphlogasides A−B(7−8)and four known analogs were isolated from the aerial parts of Phlogacanthus curviflorus(P.curviflorus).The structures of these compounds were determined using high-resolution electrospray ionization mass spectrometry(HR-ESI-MS),one-dimensional and two-dimensional nuclear magnetic resonance(NMR)spectroscopy,electronic circular dichroism(ECD)spectra,and quantum chemical calculations.Notably,compounds 5 and 6 represented the first reported instances of entnorabietane diterpenoids from the genus Phlogacanthus.In theβ-hematin formation inhibition assay,compounds 2,4,7−10,and 12 displayed antimalarial activity,with IC_(50) values of 12.97−65.01μmol·L−1.Furthermore,compounds 4,5,8,and 10 demonstrated neuroprotective activity in PC12 cell injury models induced by H_(2)O_(2) and MPP+.