[Objectives] This study was conducted to develop environmentally-friendly effective vase solutions for cut rose flowers convenient for ordinary household use. [Methods] The vase life, flowering stage, flower diameter ...[Objectives] This study was conducted to develop environmentally-friendly effective vase solutions for cut rose flowers convenient for ordinary household use. [Methods] The vase life, flowering stage, flower diameter variation, petal water content, and soluble protein and malondialdehyde contents were determined on cut rose flowers regularly during the vase period, to study the fresh-keeping effects of the vase solutions prepared from such three components as sugar, aspirin and Vc according to L9(33) orthogonal table design on cut rose flowers during the vase period. [Results] 20 g/L white granulated sugar+460 mg/L aspirin+50 mg/L Vc was the best combination in this study. [Conclusions] This study provides a reference for the research on the fresh-keeping vase solutions of household cut rose flowers.展开更多
The purpose of this paper is to investigate the differential responses of flower opening to ethylene in two cut rose cultivars, ‘Samantha’, whose opening process is promoted, and ‘Kardinal’, whose opening process ...The purpose of this paper is to investigate the differential responses of flower opening to ethylene in two cut rose cultivars, ‘Samantha’, whose opening process is promoted, and ‘Kardinal’, whose opening process is inhibited by ethylene. Ethylene production and 1- aminocyclopropane-1-carboxylate (ACC) synthase and oxidase activities were determined first. After ethylene treatment, ethylene production, ACC synthase (ACS) and ACC oxidase (ACO) activities in petals increased and peaked at the earlier stage (stage 3) in ‘Samantha’, and they were much more dramatically enhanced and peaked at the later stage (stage 4) in ‘Kardinal’ than control during vasing. cDNA fragments of three Rh-ACSs and one Rh- ACO genes were cloned and designated as Rh-ACS1, Rh-ACS2, Rh-ACS3 and Rh-ACO1 respectively. Northern blotting analysis revealed that, among three genes of ACS, ethylene-in- duced expression patterns of Rh-ACS3 gene corresponded to ACS activity and ethylene production in both cultivars. A more dramatic accumulation of Rh-ACS3 mRNA was induced by ethylene in ‘Kardinal’ than that of ‘Samantha’. As an ethylene action inhibitor, STS at concentration of 0.2 mmol/L generally inhib-ited the expression of Rh-ACSs and Rh-ACO in both cultivars, although it induced the expression of Rh-ACS3 transiently in ‘Kardinal’. Our results suggests that ‘Kardinal’ is more sensitive to ethylene than ‘Samantha’; and the changes of Rh-ACS3 expression caused by ethylene might be related to the acceleration of flower opening in ‘Samantha’ and the inhibition in ‘Kardinal’. Additional results indicated that three Rh-ACSs genes were differentially associated with flower opening and senescence as well as wounding.展开更多
文摘[Objectives] This study was conducted to develop environmentally-friendly effective vase solutions for cut rose flowers convenient for ordinary household use. [Methods] The vase life, flowering stage, flower diameter variation, petal water content, and soluble protein and malondialdehyde contents were determined on cut rose flowers regularly during the vase period, to study the fresh-keeping effects of the vase solutions prepared from such three components as sugar, aspirin and Vc according to L9(33) orthogonal table design on cut rose flowers during the vase period. [Results] 20 g/L white granulated sugar+460 mg/L aspirin+50 mg/L Vc was the best combination in this study. [Conclusions] This study provides a reference for the research on the fresh-keeping vase solutions of household cut rose flowers.
基金This work was supported by the Nat ional Natural Science Foundation of China(Grant No.30471220).
文摘The purpose of this paper is to investigate the differential responses of flower opening to ethylene in two cut rose cultivars, ‘Samantha’, whose opening process is promoted, and ‘Kardinal’, whose opening process is inhibited by ethylene. Ethylene production and 1- aminocyclopropane-1-carboxylate (ACC) synthase and oxidase activities were determined first. After ethylene treatment, ethylene production, ACC synthase (ACS) and ACC oxidase (ACO) activities in petals increased and peaked at the earlier stage (stage 3) in ‘Samantha’, and they were much more dramatically enhanced and peaked at the later stage (stage 4) in ‘Kardinal’ than control during vasing. cDNA fragments of three Rh-ACSs and one Rh- ACO genes were cloned and designated as Rh-ACS1, Rh-ACS2, Rh-ACS3 and Rh-ACO1 respectively. Northern blotting analysis revealed that, among three genes of ACS, ethylene-in- duced expression patterns of Rh-ACS3 gene corresponded to ACS activity and ethylene production in both cultivars. A more dramatic accumulation of Rh-ACS3 mRNA was induced by ethylene in ‘Kardinal’ than that of ‘Samantha’. As an ethylene action inhibitor, STS at concentration of 0.2 mmol/L generally inhib-ited the expression of Rh-ACSs and Rh-ACO in both cultivars, although it induced the expression of Rh-ACS3 transiently in ‘Kardinal’. Our results suggests that ‘Kardinal’ is more sensitive to ethylene than ‘Samantha’; and the changes of Rh-ACS3 expression caused by ethylene might be related to the acceleration of flower opening in ‘Samantha’ and the inhibition in ‘Kardinal’. Additional results indicated that three Rh-ACSs genes were differentially associated with flower opening and senescence as well as wounding.
