[Objective] This study aimed to select suitable cultivars of cut roses with prominent comprehensive performance which is suitable for the commercial promotion in the tropical region of South Hainan Province,including ...[Objective] This study aimed to select suitable cultivars of cut roses with prominent comprehensive performance which is suitable for the commercial promotion in the tropical region of South Hainan Province,including Sanya and the low-altitude area.[Method] Introduction experiment and production test were designed to observe the agronomic and economic traits of cultivars in a rose resources nursery established in Sanya.[Result] Through observation in the resources garden,18 varieties were chosen to conduct the introduction experiment,of which 8 varieties were selected to accomplish the production test.The results of the production test showed that 'Vendela','Golden Emblem','Samantha' and 'Tineke' showed the best adaptability;the cut flower quality of 'Carola','Black Magic' and 'Golden Emblem' belonged to the first grade;the yield of 'Movie Star','Golden Emblem' and 'Black Magic' was more than one million per hm2;except 'Golden Emblem',the vase life of the other seven cultivars was more than 10 days;'Carola','My Choice' and 'Perfume White' had better disease-and insect-resistance;'Carola','Vendela','Rouge Meilland','Tineke','Golden Emblem','Samantha','Perfume White','Saiun','Asagumo' and 'Double Delight' were more resistant to blackspot,while 'Black Magic','Versilla','Alec's Red' and 'Movie Star' were less resistant to blackspot.[Conclusion] Cultivars of cut roses were able to grow and develop normally in the tropical coastal area of Hainan Province,including South Hainan and Sanya,where it was appropriate for the production of cut roses.However,it is necessary to pay attention to the prevention and control of thrips and blackspot.'Black Magic','Carola','Vendela','Rouge Meilland','Golden Emblem','Tineke','Movie Star' and 'Samantha' showed best comprehensive performance,which were thus worthy of large-scale popularization in Hainan Province.展开更多
The purpose of this paper is to investigate the differential responses of flower opening to ethylene in two cut rose cultivars, ‘Samantha’, whose opening process is promoted, and ‘Kardinal’, whose opening process ...The purpose of this paper is to investigate the differential responses of flower opening to ethylene in two cut rose cultivars, ‘Samantha’, whose opening process is promoted, and ‘Kardinal’, whose opening process is inhibited by ethylene. Ethylene production and 1- aminocyclopropane-1-carboxylate (ACC) synthase and oxidase activities were determined first. After ethylene treatment, ethylene production, ACC synthase (ACS) and ACC oxidase (ACO) activities in petals increased and peaked at the earlier stage (stage 3) in ‘Samantha’, and they were much more dramatically enhanced and peaked at the later stage (stage 4) in ‘Kardinal’ than control during vasing. cDNA fragments of three Rh-ACSs and one Rh- ACO genes were cloned and designated as Rh-ACS1, Rh-ACS2, Rh-ACS3 and Rh-ACO1 respectively. Northern blotting analysis revealed that, among three genes of ACS, ethylene-in- duced expression patterns of Rh-ACS3 gene corresponded to ACS activity and ethylene production in both cultivars. A more dramatic accumulation of Rh-ACS3 mRNA was induced by ethylene in ‘Kardinal’ than that of ‘Samantha’. As an ethylene action inhibitor, STS at concentration of 0.2 mmol/L generally inhib-ited the expression of Rh-ACSs and Rh-ACO in both cultivars, although it induced the expression of Rh-ACS3 transiently in ‘Kardinal’. Our results suggests that ‘Kardinal’ is more sensitive to ethylene than ‘Samantha’; and the changes of Rh-ACS3 expression caused by ethylene might be related to the acceleration of flower opening in ‘Samantha’ and the inhibition in ‘Kardinal’. Additional results indicated that three Rh-ACSs genes were differentially associated with flower opening and senescence as well as wounding.展开更多
基金Supported by the National Agricultural Sci-Tech Achievements Transformation Projects(2010GB2E200369)the National Spark Program Project(2011GA800004)~~
文摘[Objective] This study aimed to select suitable cultivars of cut roses with prominent comprehensive performance which is suitable for the commercial promotion in the tropical region of South Hainan Province,including Sanya and the low-altitude area.[Method] Introduction experiment and production test were designed to observe the agronomic and economic traits of cultivars in a rose resources nursery established in Sanya.[Result] Through observation in the resources garden,18 varieties were chosen to conduct the introduction experiment,of which 8 varieties were selected to accomplish the production test.The results of the production test showed that 'Vendela','Golden Emblem','Samantha' and 'Tineke' showed the best adaptability;the cut flower quality of 'Carola','Black Magic' and 'Golden Emblem' belonged to the first grade;the yield of 'Movie Star','Golden Emblem' and 'Black Magic' was more than one million per hm2;except 'Golden Emblem',the vase life of the other seven cultivars was more than 10 days;'Carola','My Choice' and 'Perfume White' had better disease-and insect-resistance;'Carola','Vendela','Rouge Meilland','Tineke','Golden Emblem','Samantha','Perfume White','Saiun','Asagumo' and 'Double Delight' were more resistant to blackspot,while 'Black Magic','Versilla','Alec's Red' and 'Movie Star' were less resistant to blackspot.[Conclusion] Cultivars of cut roses were able to grow and develop normally in the tropical coastal area of Hainan Province,including South Hainan and Sanya,where it was appropriate for the production of cut roses.However,it is necessary to pay attention to the prevention and control of thrips and blackspot.'Black Magic','Carola','Vendela','Rouge Meilland','Golden Emblem','Tineke','Movie Star' and 'Samantha' showed best comprehensive performance,which were thus worthy of large-scale popularization in Hainan Province.
基金This work was supported by the Nat ional Natural Science Foundation of China(Grant No.30471220).
文摘The purpose of this paper is to investigate the differential responses of flower opening to ethylene in two cut rose cultivars, ‘Samantha’, whose opening process is promoted, and ‘Kardinal’, whose opening process is inhibited by ethylene. Ethylene production and 1- aminocyclopropane-1-carboxylate (ACC) synthase and oxidase activities were determined first. After ethylene treatment, ethylene production, ACC synthase (ACS) and ACC oxidase (ACO) activities in petals increased and peaked at the earlier stage (stage 3) in ‘Samantha’, and they were much more dramatically enhanced and peaked at the later stage (stage 4) in ‘Kardinal’ than control during vasing. cDNA fragments of three Rh-ACSs and one Rh- ACO genes were cloned and designated as Rh-ACS1, Rh-ACS2, Rh-ACS3 and Rh-ACO1 respectively. Northern blotting analysis revealed that, among three genes of ACS, ethylene-in- duced expression patterns of Rh-ACS3 gene corresponded to ACS activity and ethylene production in both cultivars. A more dramatic accumulation of Rh-ACS3 mRNA was induced by ethylene in ‘Kardinal’ than that of ‘Samantha’. As an ethylene action inhibitor, STS at concentration of 0.2 mmol/L generally inhib-ited the expression of Rh-ACSs and Rh-ACO in both cultivars, although it induced the expression of Rh-ACS3 transiently in ‘Kardinal’. Our results suggests that ‘Kardinal’ is more sensitive to ethylene than ‘Samantha’; and the changes of Rh-ACS3 expression caused by ethylene might be related to the acceleration of flower opening in ‘Samantha’ and the inhibition in ‘Kardinal’. Additional results indicated that three Rh-ACSs genes were differentially associated with flower opening and senescence as well as wounding.