Vegetative propagation of Litsea monopetala, a wild tropical medicinal plant: Effects of indole-3-butyric acid (IBA) on stem cuttings

In this study we investigated the rooting ability and the growth performance of juvenile single-node leafy stem cuttings of Litsea monopetala （Roxb） Pers. collected from two mature mother trees preserved in the hill forest of Chittagong district, Bangladesh. The rooting ability of cuttings was studied under 0%, 0.1%, 0.2% and 0.4% indole-3-butyric acid （IBA） treatments. Significantly better rooting response （p ≤ 0.05） was observed with 0.1% IBA compared to control （0% IBA）. The mean number of roots and the length of the longest root of cuttings in different treatments showed no significant difference （p≤0.05）. After transfer into polythene bags from non-mist propagator, rooted cuttings treated with 0%, 0.1% and 0.2% IBA demonstrated the highest （100 ± 0.00%） survival capacity. The mean number of shoots developed in cuttings in the polythene bags in first three weeks varied significantly （p≤0.05） among the treatments. Effects of three fertilizer treatments, viz. T0 （no fertilizer）, T1 （10g Urea, 20g TSP, 10g MOP dissolved in 1 L water） and T2 （10g Urea, 20g TSP, 10g MOP dissolved in 2 L water） on initial growth ofstecklings were also measured over a 90-days period. The increment of leaf area of stecklings was significantly higher （p≤0.05） under T0 compared with that under T1 and T2 while the increment of stem length, collar diameter and root biomass varied insignificantly among different fertilizer treatments. The results suggest that rooting juvenile single-node leafy stem cuttings could be an effective mean of regenerating L. monopetala. The application of 0.1% IBA concentration is recommended for rooting of juvenile leafy stem cuttings and application of fertilizer appeared unnecessary for the subsequent growth of stecklings in polythene bags.

Vegetative propagation ofAesculus indica through stem cuttings treated with plant growth regulators

Cuttage propagation of Aesculus indica was tested by treatments with different concentrations of indole-3-acetic acid （IAA） @ 2000, 4000 （ppm）, indolebutyric acid （IBA） @ 2000, 4000 （ppm） and naphthlcetic acid （NAA） @ 2000, 4000 （ppm） in dry formulation in the Forest Nursery, Faculty of Forestry, SKUAST-K, Shalimar. The cuttings treated with IBA @ 4000 ppm and IBA @ 2000 ppm had a sprouting rate of 75% and 50%, respectively, which was significantly higher than that of control and other treatments. The highest rooting rate （50%） was recorded in the cuttings with the application of IBA @ 4000 ppm. The cuttings treated with IBA @ 2000 ppm had 25% rooting rate. All other treatments along with control （talc powder） failed to induce rooting. It was concluded that IBA @ 4000 ppm was a better-applied concentration for vegetative propagation ofA. indica under Kashmir conditions.

Vegetative propagation of Aesculus indica through stem cuttings treated with plant growth regulators

Cuttage propagation of Aesculus indica was tested by treatments with different concentrations of indole-3-acetic acid(IAA) @ 2000, 4000(ppm), indolebutyric acid(IBA) @ 2000, 4000(ppm) and naphthlcetic acid(NAA) @ 2000, 4000(ppm) in dry formulation in the Forest Nursery, Faculty of Forestry, SKUAST-K, Shalimar.The cuttings treated with IBA @ 4000 ppm and IBA @ 2000 ppm had a sprouting rate of 75% and 50%, respectively, which was significantly higher than that of control and other treatments.The highest rooting rate(50%) was recorded in the cuttings with the application of IBA @ 4000 ppm.The cuttings treated with IBA @ 2000 ppm had 25% rooting rate.All other treatments along with control(talc powder) failed to induce rooting.It was concluded that IBA @ 4000 ppm was a better-applied concentration for vegetative propagation of A.indica under Kashmir conditions.

Successful vegetative propagation by stem cuttings of Tetraena mongolica Maxim.

Techniques have been developed for the vegetative propagation of Tetraena mongolica,a rare and endangered species endemic to the western Ordos Plateau in the northcentral portion of China.The purpose of the study was to investigate the rooting characteris-tics of the plant species from the aspects of length and ages of cuttings,external hormone concentration,leaf retention,cutting orientations,and cutting positions.The results obtained from the experiments indicated that the rooting ratio of 2-year or 3-year old cuttings was larger than more than of 4-year old cuttings.The influence of external hormone concentration on rooting was ob-vious;the rooting ratio of cuttings was increased by external hormone treatment,especially when treated by ABT1(ABT No.1 Rooting Powder) 50 mg/L for 3 hours,in which case,the rooting ratio reached 91 percent.Leaf retention influenced rooting sig-nificantly;the rooting rate of cuttings became greater as the leaf retention increased.There was no significant difference in rooting capability between south and north orientations of the ortets.The rooting ratio of the cuttings obtained from the distal portions of shoots was significantly larger than those obtained from the middle and the basal portions of the shoots.On day three after treat-ment,calluses started appearing at the incisions of some cuttings dipped in 500 mg/L IAA(indole acetic acid).Most cuttings were healed or swelled,and 6 percent of cuttings rooted on day 6 after treatment.Higher rooting period occurred 7-10 days after the experiment was set up.

Vegetative propagation of Holarrhena pubescens,a wild tropical medicinal plant:effect of indole-3-butyric acid (IBA) on stem cuttings

The present study investigated the rooting ability and growth performance of juvenile single-node leafy stem cuttings of Holarrhena pubescens, collected from two mature mother trees preserved in the hill forests of Chittagong District, Bangladesh. The rooting ability of cuttings was studied with 0, 0.1%, 0.2% and 0.4% indole-3-butyric acid （IBA） treatments. The better rooting response was observed with 0.1% IBA, but was insignificant compared to other treatments. The mean number of roots and length of the longest root of cuttings in different treatments showed no significant difference. After transfer to the polythene bags from a nonmist propagator, rooted cuttings treated with 0.4% IBA demonstrated the highest （50.00± 0.78%） survival capacity. Regarding initial growth of established rooted cuttings （stecklings） over a 90 d period, the increment of stem length of stecklings under T2 （10 g Urea, 20 g TSP, 10 g MOP dissolved in 2 L water） and leaf area expansion of stecklings under T1 （10 g Urea, 20 g TSP, 10 g MOP dissolved in 1 L water） was significantly higher （p 〈 0.05） compared with that under TO （no fertilizer）. The increment of collar diameter and root biomass production varied insignificantly among different fertilizer concentrations, although stecklings under T2 showed the better performance. The results suggest that rooting juvenile single-node leafy stem cuttings can be an effective mean of regenerating H. pubescens. The application of 0.1% IBA or without IBA is recommended for rooting juvenile leafy stem cuttings. Application of fertilizer appears necessary for subsequent growth of stecklings in polythene bags.

Clonal propagation of guava (Psidium guajava Linn.) by stem cutting from mature stockplants

The study describes the scope of clonal propagation of guava (Psidium guajava Linn.) by stem cutting collected from mature stockplants. Cuttings were treated with 0, 0.2%, 0.4% and 0.8% IBA solution and rooted in the non-mist propagator. Rooted cuttings were allowed to grow in the polybags filled with soil and cow-dung mixed in the ratio of 3: 1 (by volume) for three months to assess the steckling capacity and initial growth performance. The study reveals that the species is amenable for clonal propagation by mature stem cutting. The highest rooting percentage (60%) was observed in the cuttings treated with 0.4% IBA solution followed by 0.2% IBA and the lowest was in controlled cuttings. The maximum number of primary root (32.7) was developed in the cuttings treated with 0.8% IBA solution followed by 0.4% IBA and the lowest was in the cuttings without IBA treatment. The highest survival percentage (70.9) was observed in the cuttings rooted with 0.4% IBA treatment and the lowest (58.3) was in the cuttings without any treatment. However, there was no significant variation in height growth of cutlings due to IBA treatments in rooting.

Rooting of Stem Cuttings of <i>Jatropha platyphylla</i>(Euphorbiaceae) in the Obtaining of Axillary Buds for Grafting

Jatropha platyphylla is considered as a potential source of edible protein, oil, and phenolic compounds with anti-inflammatory activity. The use of stem cutting in vegetative propagation and grafting is as indispensable tools for mass multiplication of superior genotypes and helps in improve planting yield and quality. The study was aimed to investigate the effect of different diameters (10 - 15, 16 - 25 and 26 - 35 mm) and different hormone concentrations of indo-butyric acid (0, 1.5, 3, 6 and 10 g/L), in the rooting of Jatropha platyphylla and to obtain axillary buds to performed grafts. Rooting efficiency was 80% in greenhouse conditions. Hormone concentration and diameter significantly affected the rooting and shooting ability of Jatropha platyphylla stem cuttings. Stem cuttings of 26 - 35 mm have the greatest number, length and dry root weight. 76% survival of the grafted plants was obtained. This demonstrates the necessity to improve the conventional propagation methods with appropriate scientific inputs for more economical and time efficient techniques for standard propagation protocols.

Effect of Mineral Acids on Rooting Response of Aging Mung Bean （Phaseolus aureus Roxb,） Cuttings via Indole Acetic Acid Level

The influence of nitric acid （HNO3） as a strong mineral acid on rooting response of fresh mad aging mung bean cuttings has been studied on the level of Indole-3-Acetic Acid （IAA）. The data revealed significant increase in rooting response of fresh cuttings treated with （0.0001% and 0.1%） concentrations of HNO3 solution, significant increase in rooting response of aged cuttings （for 3 days in d/H20） with （0.0001% and 0.01%） concentration of HNO3 solution and highly significant increase in rooting response of aged cuttings （for 3 days in HNO3 solution） with （0.0001%） concentration, while highly concentration （0.5%） revealed highly significant decrease in rooting response compared to control （d/H20）. Quantitative estimation oflAA by spectrophotometric method as indicators for oxidative processes that occurrence during aging phenomenon verified a highly significant increase of IAA content in hypocotyles of fresh and aged cuttings in optimal concentration of HNO3.

Large Scale Multiplication of Casuarina junghuhniana Miq. Clonal Plants through Mini-cutting Technique

The modern concept of meeting the customer’s requirements in better products at low costs in a sustainable manner is possible only through innovative methods.The nodal cutting technique is the most widely used method for large scale propagation of Casuarina,Eucalyptus and other pulpwood species in India.Tamil Nadu Newsprint and Papers Limited(TNPL)has started large scale multiplication of Casuarina junghuhniana Miq.using mini-cutting technique from indoor clonal mini hedges raised in sand beds.When compared to stem/nodal cuttings,indoor clonal mini hedges raised in sand beds improve the rooting potential,quality of root systems and are time-and cost-saving.The productivity of cuttings is increased five times in indoor clonal hedge orchard than conventional stem/nodal cutting.The rooting percentage also improved to 90%without rooting hormone whereas the same is only 50%in stem cutting.The plant developed through mini-cutting technique has more lateral root system which helps the plants/trees to withstand during heavy winds.Replacing such stump derived stock plants by intensively managing indoor sand bed clonal mini hedges resulted in a noticeable enhancement of cutting capacity for adventitious rooting as well as the overall quality of the plants produced in much shorter period with easier and cheaper maintenance.The study reveals that mini-cutting method is ideal propagation technique for large scale propagation of C.junghuhniana clones in India.

Effect of Leafy and Leafless Greenwood, Softwood and Hardwood Cuttings Success of <i>Garcinia kola</i>(Heckel)

Garcinia kola Heckel, called “petit cola” in Côte d’Ivoire and “bitter kola” in Nigeria, is a Non-Timber Forest Product of great socioeconomic importance. Unfortunately, this species is threatened due to overexploitation and deforestation. This study tested the effect of leaf area on stem cuttings to regenerate vegetatively in a non-mist poly-propagator without using exogenous hormones. Three stem cuttings types: greenwood, softwood and hardwood (12 - 15 cm in length) were collected from 24-month years old seedlings. The two top leaves of each cutting type were conserved at different leaf area: 0 cm2, 28.25 cm2, 56.5 cm2, 84.75 cm2 and 113 cm2 (entire leaf area). Observations were made on rooting, sprouting and leafing abilities of cuttings depending to treatments applied. Results showed that all cutting types have presented good ability to shooting and rooting. Cuttings with 113 cm2 leaf areas presented the best rooting (94.44% ± 2.42%) and shouting (95.55% ± 2.42%) percentage and longest primary root length (12.03 ± 0.50 cm). Hardwood, softwood and greenwood cuttings with 113 cm2 leaf area had the best rooting percentage (96.66% ± 3.33%, 96.66% ± 5.77% and 90.00% ± 5.77% respectively). The longest primary root length (13.50 ± 0.97 cm) was observed to greenwood cuttings with 113 cm2 leaf area. Regenerated plants in the forest presented well survival percentage (96.66%). This study shows that it is possible to regenerate entire plants by cuttings without using external hormones in non-mist poly-propagator.