Background:The selection of Marek’s disease(MD)-resistant breeds in Partridge Shank chicken,a popular local chicken breed in Henan Province of China,has practical value.We hypothesized that the two polymorphic sites(...Background:The selection of Marek’s disease(MD)-resistant breeds in Partridge Shank chicken,a popular local chicken breed in Henan Province of China,has practical value.We hypothesized that the two polymorphic sites(rs14527240 located in SMOC1 and GGaluGA156129 located in PTPN3)related to MD resistance in White Leghorn chickens are also applicable to Partridge Shank chickens.Methods:In this experiment,we screened 10 live hens and 2 live roosters with the double GG genotype by genotyping the two sites from 6500 Partridge Shank chickens.Nineteen one-day-old chicks with the double GG genotype were obtained by artificial insemination.Seventy-two one-day-old chickens(19 from the population expansion test and 53 randomly selected from chicken farms)were injected with 2000 plaque-forming units of the Md5 virus strain.After 100 days of infection,all chickens were examined by pathological anatomical examination,histological sectioning,genotyping,and a quantitative polymerase chain reaction of SMOC1 and PTPN3.Results:There was only one site(rs14527240 located in SMOC1)associated with MD in Partridge Shank chickens(p<0.05),but the GG genotype of SMOC1 in Partridge Shank chickens indicated susceptibility to MD.SMOC1 expression in MD-susceptible chickens was also significantly higher than that in MDresistant chickens(p<0.05).Conclusion:Therefore,the MD resistance sites selected from White Leghorn chickens were not completely suitable for Partridge Shank chickens,but they can be used as a reference.This study indicated that SMOC1 plays an important role in screening for MD resistance in poultry.展开更多
[Objectives]The purpose of this experiment was to study the effects of dietary supplementation of Herba Picriae extract on the growth performance and immune organ index of Guangxi partridge chickens.[Methods]A total o...[Objectives]The purpose of this experiment was to study the effects of dietary supplementation of Herba Picriae extract on the growth performance and immune organ index of Guangxi partridge chickens.[Methods]A total of 500 one-day-old Guangxi partridge chickens were selected and randomly divided into five groups,five replicates for each group and 20 chickens for each replicate.Groups A,B and C were treatment groups,in which 0.70%,0.35%and 0.175%of Herba Picriae extract was added to the basal diet,respectively.Group D was a positive drug control group,in which 0.01%colistin sulfate premix was supplemented.Group E was a blank control group,in which the chickens were fed the basal diet.The experimental period was 105 d.During the experiment,five chickens were randomly selected from each group on days 21,35 and 49,respectively for the measurement of immune organ index.[Results]When the chickens were fed basal diet supplemented with 0.175%of Herba Picriae extract,their daily body weight gain increased significantly from day 22 to day 35 and from day 71 to day 105 and tended to increase from day 1 to day 21,from day 36 to day 49,and from day 50 to day 70,with a range of 7.37%-8.72%.Dietary supplementation of 0.70%and 0.175%of Herba Picriae extract significantly promoted the growth and development of Fabricius bursae in the experimental chickens.[Conclusions]Dietary supplementation of 0.175%of Herba Picriae extract improved the growth performance and feed intake,as well as 21-d-old bursal index of the experimental chickens.展开更多
[Objective] This trial was to investigate the effect of dietary Picria felterrae Lour extracts on serum biochemical indexes and immune function of Guangxi Partridge chickens. [Method] Totally 500 individuals of 1-day-...[Objective] This trial was to investigate the effect of dietary Picria felterrae Lour extracts on serum biochemical indexes and immune function of Guangxi Partridge chickens. [Method] Totally 500 individuals of 1-day-old Guangxi Partridge chickens were randomly divided into five groups, five replicates each group and 20 chicks each replicate. Groups A, B and C were treatment groups supplemented with 0.70%, 0.35% and0.175% P. felterrae extracts in the basal diet, respectively; group D was a medical control group supplemented with 0.01% colistin sulfate premix in the basal diet; and group E was a control group with basal diet. Serum samples were collected from 10 chickens in each group at 21, 35 and 49 days of age, to analyze the biochemical and immune indexes. [Result] It had no significant impact on serum TP and GLU contents of chickens by adding different levels of P. felterrae extracts in the basal diet（P 〉0.05）. However, at 21 and 35 days of age, the TBILI content of chickens in groups A, B and C were extremely lower or significantly lower than that in group E（ P〈0.01; P〈0.05）. At 35 days of age, the serum AKP activities in groups A, B and C were extremely higher than that in group E（P〈0.01）, and the serum GOT activities at 21 and 35 days of age in group C were significantly higher than that in group E（P〈0.05）. At 49 days of age, the serum Ig G content in groups A, B and C and the IL-2 content in group B and group C were significantly lower or extremely lower than that in group E（ P〈0.05; P〈0.01）; the T-AOC content in group B was significantly higher than that in group E（P〈0.05）; the GSH content in groups A, B and C were significantly higher than that in group E（ P〈0.05）. Adding different levels of P. felterrae extracts in the diet significantly decreased the TBILI content, reduced the Ig G, IL-2 and IL-6 contents, and improved the AKP and GOT activities and the T-AOC and GSH contents of Guangxi Partridge chicken. [Conclusion] Adding 0.35% P. felterrae extracts in the basal diet received the best effort.展开更多
supported by the Earmarked Fund for the Modern Agroindustry Technology Research System, China （CARS-41）;the National High Technology Research and Development Program of China （2011AA100301）
基金supported by grants from Key Research Project of the Shennong Laboratory(Grant Number SN01-2022-05)the Tackling Key Projects in Science and Technology in Henan Province(Grant Number 162102110039)the Independent Innovation Project of Henan Academy of Agricultural Sciences(Grant Number 2022ZC46).
文摘Background:The selection of Marek’s disease(MD)-resistant breeds in Partridge Shank chicken,a popular local chicken breed in Henan Province of China,has practical value.We hypothesized that the two polymorphic sites(rs14527240 located in SMOC1 and GGaluGA156129 located in PTPN3)related to MD resistance in White Leghorn chickens are also applicable to Partridge Shank chickens.Methods:In this experiment,we screened 10 live hens and 2 live roosters with the double GG genotype by genotyping the two sites from 6500 Partridge Shank chickens.Nineteen one-day-old chicks with the double GG genotype were obtained by artificial insemination.Seventy-two one-day-old chickens(19 from the population expansion test and 53 randomly selected from chicken farms)were injected with 2000 plaque-forming units of the Md5 virus strain.After 100 days of infection,all chickens were examined by pathological anatomical examination,histological sectioning,genotyping,and a quantitative polymerase chain reaction of SMOC1 and PTPN3.Results:There was only one site(rs14527240 located in SMOC1)associated with MD in Partridge Shank chickens(p<0.05),but the GG genotype of SMOC1 in Partridge Shank chickens indicated susceptibility to MD.SMOC1 expression in MD-susceptible chickens was also significantly higher than that in MDresistant chickens(p<0.05).Conclusion:Therefore,the MD resistance sites selected from White Leghorn chickens were not completely suitable for Partridge Shank chickens,but they can be used as a reference.This study indicated that SMOC1 plays an important role in screening for MD resistance in poultry.
基金Science and Technology Major Project of Guangxi Zhuang Autonomous Region(Gui Ke AA17204052)Fundamental Research Fund for Public-welfare Scientific Research Institutions Directly under Guangxi Zhuang Autonomous Region(Gui Mu Yan Ke 2019-9)+1 种基金Guangxi Innovation Team Construction Project of National Modern Agricultural Industry Technology System(nycytxgxcxtd-19)Guangxi Science and Technology Base Special Project(20182023-1).
文摘[Objectives]The purpose of this experiment was to study the effects of dietary supplementation of Herba Picriae extract on the growth performance and immune organ index of Guangxi partridge chickens.[Methods]A total of 500 one-day-old Guangxi partridge chickens were selected and randomly divided into five groups,five replicates for each group and 20 chickens for each replicate.Groups A,B and C were treatment groups,in which 0.70%,0.35%and 0.175%of Herba Picriae extract was added to the basal diet,respectively.Group D was a positive drug control group,in which 0.01%colistin sulfate premix was supplemented.Group E was a blank control group,in which the chickens were fed the basal diet.The experimental period was 105 d.During the experiment,five chickens were randomly selected from each group on days 21,35 and 49,respectively for the measurement of immune organ index.[Results]When the chickens were fed basal diet supplemented with 0.175%of Herba Picriae extract,their daily body weight gain increased significantly from day 22 to day 35 and from day 71 to day 105 and tended to increase from day 1 to day 21,from day 36 to day 49,and from day 50 to day 70,with a range of 7.37%-8.72%.Dietary supplementation of 0.70%and 0.175%of Herba Picriae extract significantly promoted the growth and development of Fabricius bursae in the experimental chickens.[Conclusions]Dietary supplementation of 0.175%of Herba Picriae extract improved the growth performance and feed intake,as well as 21-d-old bursal index of the experimental chickens.
基金Supported by Science and Technology Development Program of Nanning City(20155181)Key Technology Research and Development Program of Guangxi Province(14121003-3-3)
文摘[Objective] This trial was to investigate the effect of dietary Picria felterrae Lour extracts on serum biochemical indexes and immune function of Guangxi Partridge chickens. [Method] Totally 500 individuals of 1-day-old Guangxi Partridge chickens were randomly divided into five groups, five replicates each group and 20 chicks each replicate. Groups A, B and C were treatment groups supplemented with 0.70%, 0.35% and0.175% P. felterrae extracts in the basal diet, respectively; group D was a medical control group supplemented with 0.01% colistin sulfate premix in the basal diet; and group E was a control group with basal diet. Serum samples were collected from 10 chickens in each group at 21, 35 and 49 days of age, to analyze the biochemical and immune indexes. [Result] It had no significant impact on serum TP and GLU contents of chickens by adding different levels of P. felterrae extracts in the basal diet（P 〉0.05）. However, at 21 and 35 days of age, the TBILI content of chickens in groups A, B and C were extremely lower or significantly lower than that in group E（ P〈0.01; P〈0.05）. At 35 days of age, the serum AKP activities in groups A, B and C were extremely higher than that in group E（P〈0.01）, and the serum GOT activities at 21 and 35 days of age in group C were significantly higher than that in group E（P〈0.05）. At 49 days of age, the serum Ig G content in groups A, B and C and the IL-2 content in group B and group C were significantly lower or extremely lower than that in group E（ P〈0.05; P〈0.01）; the T-AOC content in group B was significantly higher than that in group E（P〈0.05）; the GSH content in groups A, B and C were significantly higher than that in group E（ P〈0.05）. Adding different levels of P. felterrae extracts in the diet significantly decreased the TBILI content, reduced the Ig G, IL-2 and IL-6 contents, and improved the AKP and GOT activities and the T-AOC and GSH contents of Guangxi Partridge chicken. [Conclusion] Adding 0.35% P. felterrae extracts in the basal diet received the best effort.
基金supported by the Earmarked Fund for the Modern Agroindustry Technology Research System, China (CARS-41)the National High Technology Research and Development Program of China (2011AA100301)
文摘supported by the Earmarked Fund for the Modern Agroindustry Technology Research System, China （CARS-41）;the National High Technology Research and Development Program of China （2011AA100301）
