Objective To observe the different expression of NF-kBp65 and cyclinD1 during oral carcinogenesis and to analyze the relationship between the abnormal expression of NF-kBp65 , cyclinD1, and the occurrence and developm...Objective To observe the different expression of NF-kBp65 and cyclinD1 during oral carcinogenesis and to analyze the relationship between the abnormal expression of NF-kBp65 , cyclinD1, and the occurrence and development of oral carcinogenesis. Methods The streptavidin-biotin-peroxidase （S-P） immunohistochemical method was employed to detect the expression of NF-kBp65 and cyclinD1 protein in 38 rat tongue carcinogenesis specimens induced by 4-nitroquinoline 1-oxide. Results With the progress of tongue carcinogenesis, the expression of NF-kBp65, cyclinD1 was up-regulated. In normal, mild epithelial dysplasia, moderate epithelial dysplasia, severe epithelial dysplasia, carcinoma in situ and squamous cell carcinoma （SCC）, the positive rate of NF-kBp65 was 20%, 20%, 50%, 62.5%, 50% and 83.33%, respectively. There was significant differences between normal and SCC （ P 〈 0. 05 ） ; while the level of cyclinD1 was 20%, 60%, 62. 5%, 87. 5% , 100% and 83. 33%, respec- tively. There was significant differences between normal and severe epithelial dysplasia, carcinoma in situ and SCC （ P 〈0.01 or P 〈 0. 05 ）. There was a significant correlation between the increased levels of NF-kBp65, cyclinD1 and histopathological grade. The positive expression of NF-kBp65 was also associated with cyclinD1 in SCC （ r = 0. 7353, P 〈 0. 05 ）. Conclusion The up-expression of NF-kBp65 and cyclinD1 protein may be correlated to the occurrence and the development of oral carcinoma ; activated NF-kB plays an important role in the overexpression of cyclinD1. Furthermore, NF-kB and cyclinD1 may be the useful biomarker of oral precancerous lesion.展开更多
目的:探讨胰腺癌中p21W A F1、cyclinD1和CD K4的表达意义及其相互关系。方法:应用免疫组织化学二步法检测p21W A F1、cyclinD1和CD K4在48例胰腺癌和14例慢性胰腺炎中的表达。结果:胰腺癌组织中p21W A F1、cyclinD1、CD K4的阳性表达...目的:探讨胰腺癌中p21W A F1、cyclinD1和CD K4的表达意义及其相互关系。方法:应用免疫组织化学二步法检测p21W A F1、cyclinD1和CD K4在48例胰腺癌和14例慢性胰腺炎中的表达。结果:胰腺癌组织中p21W A F1、cyclinD1、CD K4的阳性表达率分别是52.08%(25/48)、70.85%(34/48)、62.50%(30/48),同慢性胰腺炎组比较p21W A F1的表达明显降低(P<0.01),而cyclinD1和CD K4的表达明显升高(P<0.01)。高分化、无淋巴结转移、临床分期Ⅰ、Ⅱ期的胰腺癌组织中p21W A F1的阳性表达率明显高于低分化、有淋巴结转移、临床分期Ⅲ、Ⅳ期的胰腺癌(P<0.05);而cyclinD1和CD K4则相反(P<0.05)。p21W AF1的表达和cyclinD1、CDK4的表达呈明显的负相关(相关系数分别为r=-0.340,P<0.05和r=-0.571,P<0.01);cyclinD1和CDK4的表达则呈明显的正相关(r=0.450,P<0.01)。结论:p21W A F1的缺失表达和cyclinD1、CD K4的过表达在胰腺癌的发生、发展过程中起重要的协同作用。三种蛋白质主要以p21W AF1/cyclinD1/CDK4通路的方式作用于细胞的转化,参与肿瘤的形成。展开更多
基金Supported by Shanghai Science and Technology Commission,China (03JC14037)
文摘Objective To observe the different expression of NF-kBp65 and cyclinD1 during oral carcinogenesis and to analyze the relationship between the abnormal expression of NF-kBp65 , cyclinD1, and the occurrence and development of oral carcinogenesis. Methods The streptavidin-biotin-peroxidase （S-P） immunohistochemical method was employed to detect the expression of NF-kBp65 and cyclinD1 protein in 38 rat tongue carcinogenesis specimens induced by 4-nitroquinoline 1-oxide. Results With the progress of tongue carcinogenesis, the expression of NF-kBp65, cyclinD1 was up-regulated. In normal, mild epithelial dysplasia, moderate epithelial dysplasia, severe epithelial dysplasia, carcinoma in situ and squamous cell carcinoma （SCC）, the positive rate of NF-kBp65 was 20%, 20%, 50%, 62.5%, 50% and 83.33%, respectively. There was significant differences between normal and SCC （ P 〈 0. 05 ） ; while the level of cyclinD1 was 20%, 60%, 62. 5%, 87. 5% , 100% and 83. 33%, respec- tively. There was significant differences between normal and severe epithelial dysplasia, carcinoma in situ and SCC （ P 〈0.01 or P 〈 0. 05 ）. There was a significant correlation between the increased levels of NF-kBp65, cyclinD1 and histopathological grade. The positive expression of NF-kBp65 was also associated with cyclinD1 in SCC （ r = 0. 7353, P 〈 0. 05 ）. Conclusion The up-expression of NF-kBp65 and cyclinD1 protein may be correlated to the occurrence and the development of oral carcinoma ; activated NF-kB plays an important role in the overexpression of cyclinD1. Furthermore, NF-kB and cyclinD1 may be the useful biomarker of oral precancerous lesion.
文摘目的:探讨胰腺癌中p21W A F1、cyclinD1和CD K4的表达意义及其相互关系。方法:应用免疫组织化学二步法检测p21W A F1、cyclinD1和CD K4在48例胰腺癌和14例慢性胰腺炎中的表达。结果:胰腺癌组织中p21W A F1、cyclinD1、CD K4的阳性表达率分别是52.08%(25/48)、70.85%(34/48)、62.50%(30/48),同慢性胰腺炎组比较p21W A F1的表达明显降低(P<0.01),而cyclinD1和CD K4的表达明显升高(P<0.01)。高分化、无淋巴结转移、临床分期Ⅰ、Ⅱ期的胰腺癌组织中p21W A F1的阳性表达率明显高于低分化、有淋巴结转移、临床分期Ⅲ、Ⅳ期的胰腺癌(P<0.05);而cyclinD1和CD K4则相反(P<0.05)。p21W AF1的表达和cyclinD1、CDK4的表达呈明显的负相关(相关系数分别为r=-0.340,P<0.05和r=-0.571,P<0.01);cyclinD1和CDK4的表达则呈明显的正相关(r=0.450,P<0.01)。结论:p21W A F1的缺失表达和cyclinD1、CD K4的过表达在胰腺癌的发生、发展过程中起重要的协同作用。三种蛋白质主要以p21W AF1/cyclinD1/CDK4通路的方式作用于细胞的转化,参与肿瘤的形成。
