非平衡循环吸附色谱分离研究

A non-linear and non-equilibrium model of cycling chromatography was established．Computer simulation and experiment of the process of cycling chromatography showed that the productionrate of cycling chromatography was higher than that of conventional chromatography method The production rate of the cycling chromatographic separation increased with the increase of the cycling number. The use of the cycling chromatography technique would improve apparently improve the production rate if the solid／liquid interface mass transfer resistance was large or the apparent selectivity coefficients of adsorbants were small.The elution curves calculated by the model of non-linear and nonequilibrium cycling chromatography, were in good agreement with experimental elution curves of chromatography while the elution curves calculated by the model of the equilibrium cycling chromatography deviated from the experimental elution curves of chromatography.

非平衡双柱交替循环色谱分离过程模型

循环色谱是一种新颖的分离技术，它以短柱多次循环分离模拟传统的长柱单循环色谱分离，并在分离的过程中不断把已被分离的组分切割抽出，缩短了操作周期时间，提高分离产率。本文建立了非平衡双柱交替循环色谱分离过程数学模型，并进行了双柱交替循环色谱分离蔗糖和还原糖实验和计算机模拟分析。研究结果表明，使用本文提出的非平衡双柱交替循环色谱模型计算得到的流出曲线与实验得到的流出曲线吻合很好。双柱循环色谱分离操作能比采用传统的色谱操作获得更高的分离产率，产率随着循环次数的增加而增大。当体系中轴向扩散较严重、液固两相间的传质阻力较大或表观选择性系数较小时，与采用传统的单长柱色谱操作方式比较，采用双柱交替循环色谱分离方式将能更明显地改善和提高分离产率。