Natural silk from Bombyx mori has been used as medical sutures for several decades,and regenerated silk fibroin( RSF)based biomaterials have been increasingly studied in the past thirty years. However,vascular graft d...Natural silk from Bombyx mori has been used as medical sutures for several decades,and regenerated silk fibroin( RSF)based biomaterials have been increasingly studied in the past thirty years. However,vascular graft derived from silk fibroin fiber has been explored in recent several years with development of textile science and engineering. Moreover,endothelialization of vascular graft has been seen as an ideal strategy for preventing thrombosis and getting higher patency in a long term. Therefore,in the present work silk fibroin fiber vascular graft( SF) was chemically grafted with bioactive molecules such as heparin and RSF to improve the cytocompatibility. 3-aminopropyl-triethoxysilane(APTES),1-ethyl-3-(3-dimethylaminopropyl) carbodiie hydrochlide( EDC · HCl),and N-hydroxysuccinimide( NHS) have been employed as coupling agent and crosslinking agents,respectively. Microscopy and ATRFTIR were used to characterize the surface changes and the structure of the grafts after treatment,respectively. Cell culture in vitro and MTT assay were conducted to determine the improvement of cell affinity to the graft. Furthermore,mechanical properties of the grafts before and after treatment were compared. The results showed that the chemical grafting was an effective method for improving the cytocompatibility of SF without significant loss of mechanical properties.展开更多
For the surgical treatment of cardiovascular disease(CVD),there is a clear and unmet need in developing small-diameter(diameter<6 mm)vascular grafts.In our previous work,sulfated silk fibroin(SF)was successfully fa...For the surgical treatment of cardiovascular disease(CVD),there is a clear and unmet need in developing small-diameter(diameter<6 mm)vascular grafts.In our previous work,sulfated silk fibroin(SF)was successfully fabricated as a potential candidate for preparing vascular grafts due to the great cytocompatibility and hemocompatibility.However,vascular graft with single layer is difficult to adapt to the complex internal environment.In this work,polycaprolactone(PCL)and sulfated SF were used to fabricate bilayer vascular graft(BLVG)to mimic the structure of natural blood vessels.To enhance the biological activity of BLVG,nicorandil(NIC),an FDA-approved drug with multi-bioactivity,was loaded in the BLVG to fabricate NIC-loaded BLVG.The morphology,chemical composition and mechanical properties of NIC-loaded BLVG were assessed.The results showed that the bilayer structure of NIC-loaded BLVG endowed the graft with a biphasic drug release behavior.The in vitro studies indicated that NIC-loaded BLVG could significantly increase the proliferation,migration and antioxidation capability of endothelial cells(ECs).Moreover,we found that the potential biological mechanism was the activation of PI3K/AKT/eNOS signaling pathway.Overall,the results effectively demonstrated that NIC-loaded BLVG had a promising in vitro performance as a functional small-diameter vascular graft.展开更多
基金National Natural Science Foundations of China(No.51003014,No.31100682)"111 Project" Biomedical Textile Materials Science and Technology of China(No.B07024)
文摘Natural silk from Bombyx mori has been used as medical sutures for several decades,and regenerated silk fibroin( RSF)based biomaterials have been increasingly studied in the past thirty years. However,vascular graft derived from silk fibroin fiber has been explored in recent several years with development of textile science and engineering. Moreover,endothelialization of vascular graft has been seen as an ideal strategy for preventing thrombosis and getting higher patency in a long term. Therefore,in the present work silk fibroin fiber vascular graft( SF) was chemically grafted with bioactive molecules such as heparin and RSF to improve the cytocompatibility. 3-aminopropyl-triethoxysilane(APTES),1-ethyl-3-(3-dimethylaminopropyl) carbodiie hydrochlide( EDC · HCl),and N-hydroxysuccinimide( NHS) have been employed as coupling agent and crosslinking agents,respectively. Microscopy and ATRFTIR were used to characterize the surface changes and the structure of the grafts after treatment,respectively. Cell culture in vitro and MTT assay were conducted to determine the improvement of cell affinity to the graft. Furthermore,mechanical properties of the grafts before and after treatment were compared. The results showed that the chemical grafting was an effective method for improving the cytocompatibility of SF without significant loss of mechanical properties.
基金This work was supported by the National Natural Science Foundation of China(31771058,32071359,11421202,61227902 and 11120101001)NationalKey Technology R&D Program(2016YFC1100704,2016YFC1101101)+3 种基金International Joint Research Center of Aerospace Biotechnology and Medical Engineering from Ministry of Science and Technology of China111 Project(B13003)Research Fund for the Doctoral Program of Higher Education of China(20131102130004)Fundamental Research Funds for the Central Universities.
文摘For the surgical treatment of cardiovascular disease(CVD),there is a clear and unmet need in developing small-diameter(diameter<6 mm)vascular grafts.In our previous work,sulfated silk fibroin(SF)was successfully fabricated as a potential candidate for preparing vascular grafts due to the great cytocompatibility and hemocompatibility.However,vascular graft with single layer is difficult to adapt to the complex internal environment.In this work,polycaprolactone(PCL)and sulfated SF were used to fabricate bilayer vascular graft(BLVG)to mimic the structure of natural blood vessels.To enhance the biological activity of BLVG,nicorandil(NIC),an FDA-approved drug with multi-bioactivity,was loaded in the BLVG to fabricate NIC-loaded BLVG.The morphology,chemical composition and mechanical properties of NIC-loaded BLVG were assessed.The results showed that the bilayer structure of NIC-loaded BLVG endowed the graft with a biphasic drug release behavior.The in vitro studies indicated that NIC-loaded BLVG could significantly increase the proliferation,migration and antioxidation capability of endothelial cells(ECs).Moreover,we found that the potential biological mechanism was the activation of PI3K/AKT/eNOS signaling pathway.Overall,the results effectively demonstrated that NIC-loaded BLVG had a promising in vitro performance as a functional small-diameter vascular graft.
