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Effect of Cycloheximide on the Adaptive Response Induced by Low Dose Radiation 被引量:4
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作者 CAI Lu AND LIu SHUZHENGDepartment of Radiation Biology, Institute of Radiation Medicine, Norman Bethune University of Medical Sciences, Changchun 130021, China Corresponding author 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 1992年第1期46-52,共7页
Human lymphocytes pre-exposed to 10 mGy or 50 mGy of X-rays become less sensitive to subsequent large dose irradiation, exhibited lower rate of chromosome aberration than expected. This adaptive response could be inhi... Human lymphocytes pre-exposed to 10 mGy or 50 mGy of X-rays become less sensitive to subsequent large dose irradiation, exhibited lower rate of chromosome aberration than expected. This adaptive response could be inhibited by cycloheximide, a protein synthesis inhibitor for successive 2 h period ranging from 0.5h before to 4h after the low dose exposure, indicating that the adaptive response was directly related with the protein synthesis. 展开更多
关键词 In Effect of cycloheximide on the Adaptive Response Induced by Low Dose Radiation
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Cycloheximide prevents production of arresting,a fraction of 30-50 kDa obtained from seminiferous tubule conditioned medium
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作者 Gustavo F.Gonzales Stella Hartinger +2 位作者 Yetssy Flores Kelly Zárate Pedro Yi 《Asian Journal of Andrology》 SCIE CAS CSCD 2004年第4期359-364,共6页
Aim: To evaluate the effect of a protein synthesis inhibitor cycloheximide on arresting activity in spermato-genesis and sperm count in male rats. Methods: The study used seminiferous tubule (ST) segments from adult r... Aim: To evaluate the effect of a protein synthesis inhibitor cycloheximide on arresting activity in spermato-genesis and sperm count in male rats. Methods: The study used seminiferous tubule (ST) segments from adult rats cultured in vitro with or without cycloheximide to condition culture media, which have been concentrated, size fractioned (30-50 kDa) and administered 7 days to adult rats by intraperitoneal injections. The effects on testicular and epididymal weights, spermatogenesis and epididymal sperm count were determined. Results: The fraction (30-50 kDa), named arresting, obtained from the culture without cycloheximide decreased testicular and epididymal weights (P<0.01) and reduced the epididymal sperm count significantly. Study of the spermatogenic cycle by transillumination showed spermatogenic arrest at stage VII in rats treated with arresting compared to that observed in controls. The length of stage VII in the group receiving the seminiferous tubules culture media with cycloheximide (30-50 KDa CHX-STCM fraction) was similar to control. Conclusion: The difference in the effect may be the result of the presence or absence of arresting, a protein secreted by the tubules. 展开更多
关键词 cycloheximide ARRESTING conditioned medium seminiferous tubule sperm production TRANSILLUMINATION
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Protective effect and its mechanism of codonopsis pilosula polysaccharide on memory consolidation disorder induced by cycloheximide in mice
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作者 Wei LI Wei-da LIU +2 位作者 Li-yao DUAN Jiang-man LI Dan-shen ZHANG 《中国药理学与毒理学杂志》 CAS CSCD 北大核心 2017年第10期992-992,共1页
OBJECTIVE To investigate the protective effect of Codonopsis Pilosula Polysaccharide(CPPS)on improving of the memory consolidation disorder induced by Cycloheximide and its possible mechanisms in mice.METHODS The mice... OBJECTIVE To investigate the protective effect of Codonopsis Pilosula Polysaccharide(CPPS)on improving of the memory consolidation disorder induced by Cycloheximide and its possible mechanisms in mice.METHODS The mice was divided into five groups,as normal control group,cycloheximid model group,piracetam positive control group,CPPS 300 mg·kg^(-1) group,and CPPS150 mg·kg^(-1) group.The mice respectively were given saline,piracetam,and CPPS for 15 d.The memory consolidation disorder model in mice was established by ip.Cyclohexylamine,and orally administered CPPS(300 mg·kg^(-1) or 150 mg·kg^(-1))every day.Then experimental groups were subjected Morris Water Maze test.Western blotting analysis were used to analysis the expression of Ca MKⅡ/CREB signaling pathways.RESULTS Morris water maze experiment showed that cyclohexylamine can cause memory consolidation disorder(P<0.01),and giving piracetam and CPPS(300 mg·kg-1)can improve spatial memory impairment in mice(P<0.05,P<0.01).Western blotting experiment results show that compared with normal control group,Ca MKⅡand CREB contents of brain in model group mice had significant decreased(P<0.001);Compared with model group,Ca MKⅡand CREB contents of brain tissue in piracetam and CPPS groups increased significantly(P<0.05,P<0.01,P<0.001).CONCLUSION Cycloheximide can induce the memory consolidation disorder,and its effect in mice related to Ca MK/CREB signaling pathways.CPPS can improved this memory disorder by influence Ca MKⅡ/CREB signaling pathways. 展开更多
关键词 codonopsis pilosula polysaccharide cycloheximide memory consolidation disorder Morris water maze CaMKⅡ/CREB signaling pathways
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Himalomycin A and cycloheximide-producing marine actinomycete from Lagos Lagoon soil sediment
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作者 Davies Olabisi Flora Adeleye Isaac Adeyemi Wang Peng George 《Journal of Coastal Life Medicine》 2015年第5期361-365,共5页
Objective:To isolate and screen Actinomycetes from Lagos Lagoon soil sediments for antibiotic production.Methods:Soil samples were collected from four different locations of Lagos Lagoon and were dried for 2 weeks.Act... Objective:To isolate and screen Actinomycetes from Lagos Lagoon soil sediments for antibiotic production.Methods:Soil samples were collected from four different locations of Lagos Lagoon and were dried for 2 weeks.Actinomycetes were isolated by serial dilution using spread plate method on starch casein and Kuster’s agar supplemented with 80μg/mL cycloheximide to prevent fungal growth.The plates were incubated at 28°C for 1-2 weeks.Isolates were selected based on their cultural characteristics as well as their Gram’s reaction and subcultured on same media for isolation and incubated at 28°C for 3 days.Pure cultures were maintained on nutrient agar slants at 4°C.Thereafter,they were inoculated into starch casein and Kuster’s broth media and incubated at 28°C for 8 days.The resulting crude extracts were screened for antimicrobial activity against the following microorganisms:methicillin resistant Staphylococcus aureus,Staphylococcus aureus ATCC 29213,Escherichia coli ATCC 29522,Pseudomonas aeruginosa ATCC 27853,Candida albicans and Enterococcus faecalis ATCC 29212.Coagulase-negative staphylococci isolated from HIV patients were also used(Staphylococcus warneri,Staphylococcus xylosus and Staphylococcus epidermidis).Extraction of secondary metabolites was carried out and analysed using gas chromatography-mass spectrometer.Results:All the isolates displayed varying antimicrobial activity against at least one of the test organisms.Himalomycin A was identified in the extract from isolate ULS7.The gas chromatography-mass spectrometer data analysis showed the antibiotic profile of these isolates.Conclusions:The isolate ULS7 was found to display the highest antimicrobial activity against the test organisms. 展开更多
关键词 Marine ACTINOMYCETES STREPTOMYCES Himalomycin A cycloheximide Molecular identification Morphological characteristics Gaschromatography-mass SPECTROMETER
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RESPONSES TO ETHYLENE AND PROTEIN SYNTHESIS OF CARNATION FLOWERS 被引量:4
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作者 姜微波 Shimon Mayak Abraham H.Halevy 《Acta Botanica Sinica》 CSCD 1997年第11期991-997,共7页
The symptoms of petal response to ethylene, including petal in-rolling and increase in ion leakage through cell membrane, were completely inhibited by treatment of transcription inhibitor a-amanitin as well as protein... The symptoms of petal response to ethylene, including petal in-rolling and increase in ion leakage through cell membrane, were completely inhibited by treatment of transcription inhibitor a-amanitin as well as protein synthesis inhibitor--cycloheximide. It was observed in Dianthus caryophyllus L. cv. White Sim that protein synthesis in flowers declined and the responsiveness to ethylene increased as the flowers became matured. However, when the flowers were pre-treated with aminooxyacetic acid (AOA), an inhibitor of ethylene synthesis, the observed responsiveness to ethylene in flowers declined with age. These results suggested that responsiveness to ethylene in flowers may be due to a declination in the capacity of protein synthesis in flowers. 展开更多
关键词 CARNATION ETHYLENE Protein synthesis a-amanitin cycloheximide
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Isolation of Secondary Metabolites with Antimicrobial Activities from Bacillus amyloliquefaciens LWYZ003 被引量:3
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作者 Zhen Liu Yiting Wang +1 位作者 Xiaoqiang Jia Wenyu Lu 《Transactions of Tianjin University》 EI CAS 2019年第1期38-44,共7页
The strain LWYZ003, which can restrain multiple pathogens, was screened from the sediment of the ocean and identified as Bacillus amyloliquefaciens. Large-scale fermentation and modern chromatographic separation techn... The strain LWYZ003, which can restrain multiple pathogens, was screened from the sediment of the ocean and identified as Bacillus amyloliquefaciens. Large-scale fermentation and modern chromatographic separation technologies(macroporous resin column chromatography, silica gel column chromatography, thin-layer chromatography and high-performance liquid chromatography) were used to separate antimicrobial products from the fermentation broth of marine-derived Bacillus amyloliquefaciens LWYZ003. Bioactive-guided separation was used in the term of seeking antimicrobial products from the secondary metabolites of Bacillus amyloliquefaciens LWYZ003. As a result, two natural products cycloheximide( 1) and trehalose( 2) were obtained. Their structures were elucidated by Fourier transform infrared spectroscopy, high-resolution mass spectrometry, ~1 H and ^(13) C nuclear magnetic resonance analysis. In the cylinder plate method, compound 1 exhibited stronger antimicrobial activities than compound 2 against Micrococcus luteus, and also exhibited wider antimicrobial spectrum than compound 2. In conclusion, isolation of bioactive secondary metabolites from marine Bacillus sp. has enormous potentials in finding suitable antibiotics to inhibit multiple pathogens. 展开更多
关键词 cycloheximide TREHALOSE BACILLUS amyloliquefaciens Bioactivity-guided ISOLATION Antibiotic
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Protective Effects of Focal Ischemic Preconditioning and HSP70 Expression on Middle Cerebral Artery Occlusion in Rats
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作者 赵建华 孙圣刚 陈小武 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2006年第4期436-439,共4页
Summary: To systematically evaluate the importance of protein synthesis in ischemic preconditioning (PC)-induced ischemic tolerance (IT), temporary middle cerebral artery occlusion (MCAO) by Longa (20 min) wa... Summary: To systematically evaluate the importance of protein synthesis in ischemic preconditioning (PC)-induced ischemic tolerance (IT), temporary middle cerebral artery occlusion (MCAO) by Longa (20 min) was used for PC (ischemic precondioning). Twenty-four hours of reperfusion was allowed after PC and before permanent MCAO to establish ischemic tolerance (IT) to compare with non-PC (sham-operated) rats (n=5 for each group). Infarct size and neurological deficits were measured 24 h after PMCAO. Samples of brain were taken for the determination of HSP70 expression by Western blot analysis. The effects of the protein synthesis inhibitor cycloheximide administered just before PC or administered long after PC but just before PMCAO on IT were also determined (n=5 for each group). Our results showed that hemispheric infarct was significantly reduced (P〈0.01) only if PC was performed after 24 h, and PC significantly (P〈0.05) reduced neurological deficits (similar to reductions in infarct size). Cycloheximide eliminated ischemic PC-induced IT effects on both brain injury and neurological deficits if administered before PC but not if administered long after PC but before PMCAO. PC produced no brain injury but did increase HSP70 protein 24 h after PC. Cycloheximide eliminated that effect. The results suggest that PC is a powerful inducer of ischemic brain tolerance as reflected by the preservation of brain tissue and motor function. PC induces IT that is dependent on de novo protein synthesis. 展开更多
关键词 ischemic precondioning ischemic tolerance brain infarct HSP70 cycloheximide
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白刺链霉菌(Streptomyces albospinus)CT205产环己酰亚胺含量测定方法的建立
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作者 王小姣 李梦雅 王世梅 《微生物学杂志》 CAS CSCD 2020年第6期96-101,共6页
建立白刺链霉菌(Streptomyces albospinus)CT205代谢产环己酰亚胺含量的检测方法,为菌株CT205的开发利用提供技术支持。采用高效液相色谱法(HPLC)、生物活性法和分光光度法等三种检测手段分别测定菌株CT205发酵上清液及粗提物中活性物... 建立白刺链霉菌(Streptomyces albospinus)CT205代谢产环己酰亚胺含量的检测方法,为菌株CT205的开发利用提供技术支持。采用高效液相色谱法(HPLC)、生物活性法和分光光度法等三种检测手段分别测定菌株CT205发酵上清液及粗提物中活性物质环己酰亚胺(Cycloheximide)的含量。三种方法测定环己酰亚胺标准品均具有较好的线性关系,其中相关系数R_(HPLC)^(2)法(0.9992)>R_(生物活性法)^(2)(0.9937)≈R_(分光光度法)^(2)(0.9965)。HPLC法及生物活性法分别测定CT205发酵液及粗提物中活性物质含量分别为72.87、1555.70及66.15、1259.00μg/mL,HPLC法与生物活性法测定的发酵上清液中活性物质含量误差在+6.72μg/mL。HPLC检测方法的准确性及灵敏度均高于生物活性法,适用于样品含量的准确测定,生物活性法适用于菌株诱变筛选及条件优化实验产生的大批量样品的测定及比较,分光光度法不适用检测杂质含量较多的样品。 展开更多
关键词 白刺链霉菌(Streptomyces albospinus)CT205 环己酰亚胺(cycloheximide) 高效液相色谱法 生物活性法 分光光度法
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Induction of apoptosis of lymphocytes in ratmucosalimmune system 被引量:5
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作者 CHEN Xue-Qing ZHANG Wan-Dai +1 位作者 SONG Yu-Gang ZHOU Dian-Yuan 《World Journal of Gastroenterology》 SCIE CAS CSCD 1998年第1期24-28,共5页
IM To undergo apoptosis during negative and positive selection processes in rat mucosal immune system which are implicated in the pathogenesis of various mucosal diseases. METHODS Female SpragueDawley rats were g... IM To undergo apoptosis during negative and positive selection processes in rat mucosal immune system which are implicated in the pathogenesis of various mucosal diseases. METHODS Female SpragueDawley rats were given protein synthesis inhibitor, cycloheximide, intravenously or intraperitoneally, an apoptosis was recognized by morphological hallmark under light and electronmicroscopy, and the expression of proliferating cell nuclear antigen was visualized immunohistochemically. RESULTS The apoptosis of mucosal lymphocytes in the digestive tract, as well as in trachea, uterus and lacrimal gland was induced by cycloheximide (>10mg·kg-1 body weight), which were located mainly in lamina propria and germinal centers of lymphoid nodules. At the same time, a portion of crypt epithelial cells of proliferating zone in small and large intestine, and the epithelial cells in genital tract were also found to undergo apoptosis. Immunostainings showed that apoptotic cells expressed proliferating cell nuclear antigen. CONCLUSION Apoptosis of lymphocytes in mucosal immune system can be induced by cycloheximide. This model will facilitate the understanding of normal mucosal immune system and its role in the pathogenesis of related diseases such as inflammatory bowel diseases. 展开更多
关键词 mucosal immune system cycloheximide proliferating cell nuclear antigen IMMUNOHISTOCHEMISTRY
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EFFECT OF DEXAMETHASONE ON MACROPHAGE ACTIVATION
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作者 董钟云 卢珊 张友会 《Chinese Science Bulletin》 SCIE EI CAS 1991年第5期409-413,共5页
It has been known for years that steroids (e. g. hydrocortisone and dexamethasone) have significant inhibitory effects on macrophage (M φ) activation for tumor cytotoxicity and secretion of interleukin 1, tumor n... It has been known for years that steroids (e. g. hydrocortisone and dexamethasone) have significant inhibitory effects on macrophage (M φ) activation for tumor cytotoxicity and secretion of interleukin 1, tumor necrosis factor-α(TNF-α) and prostaglandin E<sub>2</sub>. The mechanism of inhibition is, however, not well understood. We report here that the dexamethasone-induced inhibition of M φ activation for tumor cytotoxicity could be partially antagonized by high concentrations of activators and blocked by inhibitors of protein synthesis and energy metabolism. 展开更多
关键词 mcrophage DEXAMETHASONE CYTOTOXIC ACTIVITY cycloheximide SODIUM azide.
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