The present study was undertaken to evaluate the influence of the methanolic fruit extract of Momordica cymbalaria （MFMC） on PPARγ, （Peroxisome Proliferator Activated Receptor gamma） and GLUT-4 （Glucose transpor...The present study was undertaken to evaluate the influence of the methanolic fruit extract of Momordica cymbalaria （MFMC） on PPARγ, （Peroxisome Proliferator Activated Receptor gamma） and GLUT-4 （Glucose transporter-4） with respect to glucose transport. Various concentrations of MFMC ranging from 62.5 to 500 μg·mL^-1 were evaluated for glucose uptake activity in vitro using L6 myotubes, rosiglitazone was used as a reference standard. The MFMC showed significant and dose-dependent increase in glucose uptake at the tested concentrations, further, the glucose uptake activity of MFMC （500 μg·mL^-1） was comparable with rosigilitazone. Furthermore, MFMC has shown up-regulation of GLUT-4 and PPARy gene expressions in L6 myotubes. In addition, the MFMC when incubated along with cycloheximide （CHX）, which is a protein synthesis inhibitor, has shown complete blockade of glucose uptake. This indicates that new protein synthesis is required for increased GLUT-4 translocation. In conclusion, these findings suggest that MFMC is enhancing the glucose uptake significantly and dose dependently through the enhanced expression of PPARμ and GLUT-4 in vitro.展开更多
基金supported by Padmashree Institute of Information Sciences,Bangalore
文摘The present study was undertaken to evaluate the influence of the methanolic fruit extract of Momordica cymbalaria （MFMC） on PPARγ, （Peroxisome Proliferator Activated Receptor gamma） and GLUT-4 （Glucose transporter-4） with respect to glucose transport. Various concentrations of MFMC ranging from 62.5 to 500 μg·mL^-1 were evaluated for glucose uptake activity in vitro using L6 myotubes, rosiglitazone was used as a reference standard. The MFMC showed significant and dose-dependent increase in glucose uptake at the tested concentrations, further, the glucose uptake activity of MFMC （500 μg·mL^-1） was comparable with rosigilitazone. Furthermore, MFMC has shown up-regulation of GLUT-4 and PPARy gene expressions in L6 myotubes. In addition, the MFMC when incubated along with cycloheximide （CHX）, which is a protein synthesis inhibitor, has shown complete blockade of glucose uptake. This indicates that new protein synthesis is required for increased GLUT-4 translocation. In conclusion, these findings suggest that MFMC is enhancing the glucose uptake significantly and dose dependently through the enhanced expression of PPARμ and GLUT-4 in vitro.
