Common carp are among the oldest domesticated fish in the world.As such,there are many food and ornamental carp strains with abundant phenotypic variations due to natural and artificial selection.Hebao red carp(HB,Cyp...Common carp are among the oldest domesticated fish in the world.As such,there are many food and ornamental carp strains with abundant phenotypic variations due to natural and artificial selection.Hebao red carp(HB,Cyprinus carpio wuyuanensis),an indigenous strain in China,is renowned for its unique body morphology and reddish skin.To reveal the genetic basis underlying the distinct skin color of HB,we constructed an improved highfidelity(HiFi) HB genome with good contiguity,completeness,and correctness.Genome structure comparison was conducted between HB and a representative wild strain,Yellow River carp(YR,C.carpio haematopterus),to identify structural variants and genes under positive selection.Signatures of artificial selection during domestication were identified in HB and YR populations,while phenotype mapping was performed in a segregating population generated by HB×YR crosses.Body color in HB was associated with regions with fixed mutations.The simultaneous mutation and superposition of a pair of homologous genes(mitfa) in chromosomes A06 and B06 conferred the reddish color in domesticated HB.Transcriptome analysis of common carp with different alleles of the mitfa mutation confirmed that gene duplication can buffer the deleterious effects of mutation in allotetraploids.This study provides new insights into genotype-phenotype associations in allotetraploid species and lays a foundation for future breeding of common carp.展开更多
A precise and reliable analytical method of high performance liquid chromatography-tandem mass spectrometry(HPLCMS/MS)was developed to measure trace levels of enrofloxacin(ENR)and its major metabolite ciprofloxacin(CI...A precise and reliable analytical method of high performance liquid chromatography-tandem mass spectrometry(HPLCMS/MS)was developed to measure trace levels of enrofloxacin(ENR)and its major metabolite ciprofloxacin(CIP)in carp tissues.Optimized chromatographic separation was obtained on a Waters Xterra MS C_(18) reversed-phase column using gradient elution with methanol and 0.1%formic acid aqueous solution including 5mmolL^(-1) of ammonium acetate.The established method was applied to study the pharmacokinetics and distribution of ENR and CIP in tissues of carp following a single oral administration in feed at a dosage of 40mgkg^(-1) bw(body weight).Data were analyzed using DAS 2.0 dynamics software,and the experimental results suggest that ENR was rapidly absorbed and extensively distributed in carp tissues through systemic circulation,and the pharmacokinetic characteristics can be described with a two-compartment model.The elimination half-lives(t_(1/2β))from muscle,liver,gill,plasma and skin were 131,160,104,132 and 310 h,respectively.The areas under the drug concentration-time curves(AUC)for these tissues were 491,972,750,249 and 706hmgkg^(-1),respectively.The maximum concentration(C_(max))values were 13,29,37,9 and 5mgkg^(-1) with peak times(t_(max))of 8,4,4,2 and 4 h,respectively.Ciprofloxacin,the active metabolite of ENR,was also detected in carp tissues,indicating that only 1.54%of de-ethylation of ENR occurs in carp.At a water temperature of 18℃,the drug withdrawal time was determined to be no less than 24 d while the carp was fed at a single dosage of 40mgkg^(-1).展开更多
To measure the 96h-LC50 values of 32 substituted benzenes to the carp and to study the relationship between quantitative structure-activity and structural parameters of chemicals. Methods The acute toxicity values of...To measure the 96h-LC50 values of 32 substituted benzenes to the carp and to study the relationship between quantitative structure-activity and structural parameters of chemicals. Methods The acute toxicity values of 32 substituted benzenes to the carp were determined in a semistatic test. The energy of the lowest unoccupied molecular orbital, and the highest occupied molecular orbital, the dipole moment and the molecular weight of substituted benzenes were calculated by the quantum chemical method MOPAC6.0. Results The range of the toxicity of studied compounds was broad, and the most toxic compound was pentachlorophenol, while the least toxic compound was 4-methylaniline. By the stepwise regression analyses, a series of Quantitative structure-activity relationships (QSAR) equations were derived from all compounds and subclasses. The equation log1/LC50=0.759logP +2.222 (R2 (adj)=0.818) was found to fit well and the average predicted percentage error was 6.16%. Conclusion The toxicity of anilines and phenols to the carp could be modeled well by logP alone, whereas the toxicity of the halogenated benzenes and nitrobenznes not containing hydroxyl or amino group can be controlled by hydrophobic and electronic factors.展开更多
Phenol, a ubiquitous component of industrial effluents, is a common pollutant of water resources and a serious threat to fish.The present work demonstrates that a significant amount of phenol is retained by various ti...Phenol, a ubiquitous component of industrial effluents, is a common pollutant of water resources and a serious threat to fish.The present work demonstrates that a significant amount of phenol is retained by various tissues of the common carp.Cyprinus carpio.and the snake-headed murrel.Channa punclatus.The rate of [^(14)C] phenol accumulation was higher in the carp than in the murrel.It is suggested that retention of phenol in the brain and ovary may seriously afiect the reproductive potential of the fish. 1990 Academic Press.Inc.展开更多
To investigate the genetic composition of the carp (Cyprinus carpio) in Dongping Lake and to provide eytogenetie data for exploration, protection and appropriate utilization of the germplasm resources, karyotype of ...To investigate the genetic composition of the carp (Cyprinus carpio) in Dongping Lake and to provide eytogenetie data for exploration, protection and appropriate utilization of the germplasm resources, karyotype of the carp was analyzed in this study. In detail, colehieine was given to the fish an intraperitoneal route 24 h after the intraperitoneal injection of phytohemagglutinin (PHA) solution to arrest cells in metaphase. The kidney cells were collected for the preparation of chromosome specimens, which were observed under a microscope. Ten cells having correct number of chromosomes and clear morphology were photographed and zoomed to measure the relative length, arm ratio, ann number (NF) and other parameters of chromosomes. The results showed that the chromosome number of C. earpio in Dongping Lake was (2n = 100) ; the karyotype formula was (2n =20m +38sm +20st +22t). The total number of chromosome arms (NF) was 158. By comparing the karyotype of Dongping Lake carp with that of other Cyprinus species, we concluded that Dongping Lake carp is more highly evolved among Cyprinus species.展开更多
Isolated ovarian tissues from the common carp, Cyprinus carpio were incubated in vitro to obtain a discrete effect of four common toxicants of industrial origin, namely phenol, sulfide, mercuric chloride and cadmium c...Isolated ovarian tissues from the common carp, Cyprinus carpio were incubated in vitro to obtain a discrete effect of four common toxicants of industrial origin, namely phenol, sulfide, mercuric chloride and cadmium chloride, on gonadotropin-induced alteration of nonesterified and esterified cholesterol and steroidogenic enzymes, △5-3β-HSD and 17β-HSD activity. Stage II ovarian tissue containing 30-40% mature oocytes were shown to be most responsive to gonadotropins in depleting only nonesterified cholesterol moiety and stimulating the activity of both. Safe doses of above mentioned toxicants when added separately to stage II ovarian tissue with oLH (1 μg/incubation) gonadotropin-induced depletion of nonesterified cholesterol and gonadotropin-induced stimulation of the activity of both enzymes was significantly inhibited. Esterified cholesterol remained almost unaltered. Findings clearly indicate the impairment of gonadotropin induced fish ovarian steroidogenesis by the four toxicants separately.展开更多
The genotoxic effects of quinalphos(QP)were evaluated in the erythrocytes of Cyprinus carpio using the comet,micronucleus,and nuclear abnormality assays.The 96 h LC_(50) value of quinalphos,estimated by static renewal...The genotoxic effects of quinalphos(QP)were evaluated in the erythrocytes of Cyprinus carpio using the comet,micronucleus,and nuclear abnormality assays.The 96 h LC_(50) value of quinalphos,estimated by static renewal system,was 10.9µL/L.On the basis of the LC_(50) value,two sublethal concentrations(1.09 and 2.18µL/L)of the compound were used together with cyclophosphamide(5 mg/L)as the positive control.The fish were exposed for 35 d,and sampling was done at the end of 7,14,21,28,and 35 d.Our results reveal a significant dose-dependent increase in the frequencies of DNA strand breaks,micronucleus,and nuclear abnormalities.Similar responses were also observed in fish exposed to the positive genotoxin cyclophosphamide.The collective findings demonstrated that quinalphos exerts a genotoxic effect on C.carpio.The results further confirmed that the comet,micronucleus,and nuclear abnormality assays are useful tools in determining the potential genotoxicity of pesticides towards aquatic organisms.展开更多
[Objectives]To study the effects of biological flocs on growth performance of Cyprinus carpio var.Furui No.2 and aquaculture water quality.[Methods]The comparative experiment method was adopted.An experimental group a...[Objectives]To study the effects of biological flocs on growth performance of Cyprinus carpio var.Furui No.2 and aquaculture water quality.[Methods]The comparative experiment method was adopted.An experimental group and a control group were set up,with 3 replicates in each group.The experimental group used beet molasses as the carbon source which was added once a week.The breed,quantity,and specifications of stocked fish in each group were exactly the same.The experiment lasted for 60 d.Fish growth indicators were measured every 7 d.After one week of the experiment,ammonia nitrogen and nitrite were monitored once a week,and dissolved oxygen was monitored once a day.[Results]The survival rate of fish,the average weight of,the weight gain rate,and the specific growth rate of the experimental group were significantly higher than those of the control group(P<0.05),and the feed conversion rate was significantly lower than that of the control group(P<0.05).One week after adding the carbon source in the experimental group,the ammonia nitrogen was lower than the control group,and after three weeks it was significantly lower than the control group(P<0.05);after adding the carbon source,the nitrite in the experimental group was lower than the control group,there was no significant difference in the first three weeks(P>0.05),and there was a significant difference after three weeks(P<0.05).The feed conversion rate of the experimental group was significantly lower than that of the control group(P<0.05).[Conclusions]Adding a carbon source can significantly reduce ammonia nitrogen,nitrite and other toxic and harmful substances in the aquaculture water,promote the growth of aquaculture fish and increase the feed utilization rate.展开更多
Gonadal function in fish, CJprinus carpio was significantly affected by sublethal doses of mercuric chloride (HgCl2 ) and cadmium chloride (CdCl2 ) in chronic (45 days ) exposure.Parameters investigated were nonesteri...Gonadal function in fish, CJprinus carpio was significantly affected by sublethal doses of mercuric chloride (HgCl2 ) and cadmium chloride (CdCl2 ) in chronic (45 days ) exposure.Parameters investigated were nonesterified (NE) and esterified (E) cholestcrol of ovary,liver and serum and ovarian 3β-Hydroxysteroid and 17β-Hydroxysteroid dehyrogenaseenzyme activity and serum and pituitary gonadotropin (GtH) levels. Both the pollutantswere able to reduce the hypothalamic extract (HE) or gonadotropin releasing hormone(GnRH) induced pituitary GtH release in vitro. Short term (96h) exposure of the fish tothe pollutants had no significant effect on the gonadal function. ln addition to thedeleterious effect of pollutants on the gonadal steroidogenesis and pituitary gonadotropin release, using [4-14C] cholesterol as a tracer it was found that for 45 days expeure, HgCl2'had an adverse effect on the transport of cholesterol from circulation to ovary.展开更多
This study was conducted to evaluate the use of cyanobacteria Arthrospira platensis and cladoceran Daphnia magna biomass as complementary protein and lipid sources in diet supplementation for common carp (Cyprinus car...This study was conducted to evaluate the use of cyanobacteria Arthrospira platensis and cladoceran Daphnia magna biomass as complementary protein and lipid sources in diet supplementation for common carp (Cyprinus carpio L.) nursery. Three experimental diets containing A. platensis and D. magna meal at different concentrations were compared to the commercial (control) diet. Each experimental diet (ED) was set to contain D. magna and A. platensis meal at a specific combination: 2% and 5%, 2% and 7%, and 4% and 5% for ED1, ED2 and ED3, respectively. The protein and lipid content of the experimental diets ranged from 43.20% to 44.60% dry weight (DW) and 10.64% to 13.42% DW, respectively;while the protein and lipid content of the control diet were 43.00% DW and 6.72% DW, respectively. After 20 days of feeding period, ED3 group obtained the highest final body weight (BW) (58.18 ± 35.24 mg), total biomass (1936 ± 1625 mg), food conversion rate (1.34 ± 0.04), and specific growth rate (12.86 ± 0.03% BW day<sup>–1</sup>) among all treatment groups (P > 0.05);while ED1 group obtained the highest survival (75.5% ± 7.47%) among all treatment groups (P > 0.05). The total bacterial count and total pathogenic Aeromonas sp. in the culture water of the treatment groups (ranged from 2.00 to 2.65 × 10<sup>5 </sup>CFU mL<sup>–1</sup> and 3.50 to 4.12 × 10<sup>3</sup> CFU mL<sup>–1</sup>, respectively) were lower compared to the water of the control group fed (3.73 × 10<sup>5</sup> and 4.70 × 10<sup>3</sup> CFU mL<sup>–1</sup>, respectively). No significant differences in physicochemical water quality parameters were observed among treatments (P > 0.05). The current study suggests that the combination of A. platensis and D. magna biomass can be used as complementary protein and lipid sources in diet supplementation for common carp larvae and can result in a comparable fish growth, survival and feed utilization in common carp culture at the nursery phase.展开更多
Polybrominated diphenyl ether has become a widespread environmental pollutant in aquatic environments.BDE-209,a prominent PBDEs found in the environment and wildlife,has been proven to cause oxidative stress,immune da...Polybrominated diphenyl ether has become a widespread environmental pollutant in aquatic environments.BDE-209,a prominent PBDEs found in the environment and wildlife,has been proven to cause oxidative stress,immune damage and endocrine system disorders in fish.However,the effects of BDE-209 on the intestinal health of fish remain unclear.This research aimed to determine the effects of BDE-209 on the growth performance,digestive enzymes activities and intestinal microbiota of common carp.After 60-day exposure,the growth performance of common carp were remarkably decreased(P<0.05).Moreover,the levels of protease,amylase and lipase activities remarkably decreased by BDE-209 exposure(P<0.05).In addition,metagenomics analysis revealed that the alpha-diversity values of intestinal microbiota were significantly decreased in BDE-209 exposure groups(P<0.05).The results also indicated that BDE-209 exposure could alter the intestinal microbial compositions.At the phylum level,the proportion of Fusobacteria was markedly reduced(P<0.05),while the proportions of Bacteroidetes and Firmicutes were significantly increased(P<0.05)along with the increasing concentrations of BDE-209.At the genus level,the proportion of Shewanella was higher(P<0.05)in BDE-209 exposure groups than in control group,while the abundance of Cetobacterium showed an opposite trend.Furthermore,the proportions of some aquatic probiotics(such as Bacillus,Rhodopseudomonas and Lactobacillus)were significantly enriched(P<0.05)in control group,and the content of short-chain fatty acid producing bacterium(such as Faecalibacterium,Bacteroides and Clostridium)were decreased along with the increasing BDE-209 concentrations,but no significant differences were found among the four groups(P>0.05).Overall,exposure to BDE-209 could alter the intestinal microbiota of common carp,which may affect their growth and threaten their health.展开更多
A poor environment increases fish’s susceptibility to myxosporean infection that can cause the death of larval fish,especially for koi fish(Cyprinus carpio).This study aimed to determine the effect of probiotics,loca...A poor environment increases fish’s susceptibility to myxosporean infection that can cause the death of larval fish,especially for koi fish(Cyprinus carpio).This study aimed to determine the effect of probiotics,local anti-parasitic drugs(kutuklin),and the chemical compound diflubenzuron treatments on the koi immune response.This study used PCR with specific primer 18S SSU rDNA and DNA sequencing to detect Myxobulus phylogenetic.The treatments were divided into 5 groups:Treatment(A)(healthy koi without treatment),(B)(infected koi without treatment),(C)(infected koi with 0.55 mL/30 L probiotics),(D)(infected koi with 1μL/g of feed kutuklin),and(E)(infected koi with 0.02 mg/5 L dimilin).Myxospore has observed with Scanning Electron Microscopy(SEM)and 4′,6-diamidino-2-phenylindole(DAPI)fluorescence staining.The histological analysis using semi-quantitative scoring methods,and flow cytometry was conducted to analyse the immune response of Cluster of differentiation 4(CD4^(+)),Cluster of differentiation 8(CD8^(+)),Tumor Necrosis Factor-alpha(TNF-α),Interferon gamma(IFN-γ)cells in the gills.Results show that the histological analysis indicated edema,hyperplasia,lamella fusion,congestion,and hypertrophy lesions in infected koi.Treatment with probiotics shows the lowest damage(30.6%).The immune responses of CD4^(+)and CD8^(+)cells to dimilin treatment were 10.54%and 16.86%,respectively.The largest TNF-αand IFN-γresponse were for the kutuklin treatment(29.26%)and probiotics treatment(8.23%).展开更多
To evaluate the protective effects of Glycyrrhiza polysaccharide(GPS) against 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD)-induced hepatotoxicity in Jian carp,the fish were fed diets containing GPS at doses of 0.1,0....To evaluate the protective effects of Glycyrrhiza polysaccharide(GPS) against 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD)-induced hepatotoxicity in Jian carp,the fish were fed diets containing GPS at doses of 0.1,0.5 and 1.0 g/kg for 60 days before an intraperitoneal injection of 0.6 μg/kg TCDD at a volume of 0.05 mL/10 g body weight.At 72 hr post-injection,blood and liver samples were taken for biochemical analysis and the fish liver samples were used for the preparation of pathological slices.The results showed that increases in alanine aminotransferase(GPT),aspartate aminotransferase(GOT),lactate dehydrogenase(LDH),and alkaline phosphatase(AKP) in serum induced by TCDD were significantly inhibited by pre-treatment with 1.0 g/kg GPS.Following the 1.0 g/kg GPS pre-treatment,total protein(TP),albumin(Alb),catalase(CAT),glutathione peroxidase(GPx),total antioxidant capacity(T-AOC) and superoxide dismutase(SOD) activities in liver tissue increased significantly,malondialdehyde(MDA) formation(P &lt; 0.05 or P &lt; 0.01) was significantly inhibited,and the expression of cytochrome P4501A(CYP1A),aryl hydrocarbon receptor 2(AHR2) and aryl hydrocarbon receptor nuclear translocator 2(ARNT2) mRNA(P &lt; 0.05) was significantly enhanced.Histological observations on fish liver were obtained by preparing paraffin tissue sections via HE staining,and the results showed that histological changes were obviously reduced by 0.5 and 1.0 g/kg GPS.GPS significantly reduced liver tissue damage caused by TCDD.Overall,these results proved the hepatoprotective effect of GPS in protecting against fish liver injury induced by TCDD,and supported the use of GPS(1.0 g/kg) as a hepatoprotective and antioxidant agent in fish.展开更多
The common carp(Cyrpinus carpio L.)is the oldest cultured and the most domesticated fish species,as well as one of the most important freshwater fishes in the world.However,scientific studies on evaluating the growth-...The common carp(Cyrpinus carpio L.)is the oldest cultured and the most domesticated fish species,as well as one of the most important freshwater fishes in the world.However,scientific studies on evaluating the growth-related quantitative traits in this fish are limited.Heritability,the most important parameter in selective breeding programs,was extensively studied for the growth-related traits.The values varied widely among the experiments and methods used because of the existence of common environmental,dominance and maternal effects.However,correlations in phenotypic and genetic levels first evaluated several years ago were limited.On the other hand,heterosis was widely reported and easily obtained for growth-related traits in the common carp.Meanwhile,genotype environment interaction and prediction of breeding values have been studied recently,and are very important in conducting selective breeding programs.The developmental quantitative genetics of growth-related traits was first analyzed in the common carp for reasonable selection during ontogeny.It is expected that genetic improvement will be achieved by carrying out direct selective breeding in the common carp.展开更多
Objective:To determine acute toxicity of abamectin(abamectin used for agricultural fields and also is a common acaridae used in farms)to common carp(Cyprinus carpio).Methods:In this research,common carps were exposed ...Objective:To determine acute toxicity of abamectin(abamectin used for agricultural fields and also is a common acaridae used in farms)to common carp(Cyprinus carpio).Methods:In this research,common carps were exposed to abamectin for 96 h.LC_(50) values of 24 h,48 h,72 h and 96 h were attained by probit analysis software SPSS Version 16.Fish were exposed to different concentrations(1,2,3,6,12 and 15 mg/L)of abamectin for 96 h and physicochemical properties of water used for these experiments were stable and every mortality was recorded daily.Results:The 96 h LC_(50)of abamectin for Cyprinus carpio was 1.243 mg/L.Conclusions:Eventually toxicity values indicated that abamectin has same toxicity in studied other specie and we can state lower value of LC_(50)for studied specie in compare with most species.展开更多
This study was conducted to evaluate the toxicological response ofp-nitrotoluene and p-nitroaniline to the key fish species, Cyprinus carpio. A freshwater fish bioassay based on the 96 h LC50 was used to estimate the ...This study was conducted to evaluate the toxicological response ofp-nitrotoluene and p-nitroaniline to the key fish species, Cyprinus carpio. A freshwater fish bioassay based on the 96 h LC50 was used to estimate the single and joint toxicity of the two chemicals. The toxicity of p-nitrotoluene was greater than that of pnitroaniline based on 96 h LCso values of 40.74 mg.L^-1 and 48.99mg.L^-1, respectively. Both compounds had moderate toxicity toward Cyprinus carpio, and this toxicity increased with the exposure duration and con- centration. Binary mixtures of the compounds were more toxic than the individual compounds at 96 h, and they acted upon partial addition. When the exposure time was longer, the toxicity increased for mixtures of compounds with the same concentration or toxicity. The results of this study suggest that exposure to a combination of these chemicals would result in a higher environmental risk in aquatic systems than exposure to either compound alone. Further research is needed to investigate the combined effects and sublethal toxicity of p-nitrotoluene and p- nitroaniline, since they are both still used in China.展开更多
Objective:To investigate the effects of cadmium(Cd)and copper(Cu)on sialic acid levels of brain and blood tissues of Cyprinus carpio.Methods:Adult carps were exposed to 0.1,0.5 mg/L Cu,0.1,0.5 and 1.0 mg/L Cd and 0.1 ...Objective:To investigate the effects of cadmium(Cd)and copper(Cu)on sialic acid levels of brain and blood tissues of Cyprinus carpio.Methods:Adult carps were exposed to 0.1,0.5 mg/L Cu,0.1,0.5 and 1.0 mg/L Cd and 0.1 mg/L Cu+0.1 mg/L Cd under static experiment conditions for 1 week.At the end of exposure period,heavy metal accumulations and sialic acid levels in blood and brain tissues of the test animals were analyzed.Results:Cu and Cd accumulated in tissues in a dramatically increasing dose-dependent manner.Sialic acids level of the fish exposed to 0.1,0.5 and 1.0 mg/L Cu and Cd and control grups for 1 week were 0.834,1.427,0.672,0.934,2.968,4.714 mg/mL respectively.The results also showed that Cu has an antagonistic effect on tissue sialic acid level.Conclusions:We propose that Cd and Cu make a complex with sialic acids of membranes in the tissues researched.This complex between metal ions and sialic acid migth account for the cellular toxicity based on Cu and Cd.展开更多
Objective:To investigate the effects of various extenders containing different cryoprotectants on post-thaw viability,motility and scanning electron microscopic study of frozen spermatozoa from carp.Methods:cryopreser...Objective:To investigate the effects of various extenders containing different cryoprotectants on post-thaw viability,motility and scanning electron microscopic study of frozen spermatozoa from carp.Methods:cryopreservation has been achieved using extender and cryoprotectants like dimethylacetamide After screening a variety of cryoprotectants and extenders,a protocol for the 5%-20%at appropriate dilution ratio(1:10).For all experimental tests,the motility and viability percentage of spermatozoa were examined after storage of 5 d at 4℃.Results:The maximum motility of 75%has been observed with 10%dimethyl sulfoxide.Scanning electron microscopic studies on normal and cryopreserved spermatozoa showed prominent head,middle piece and different segments of flagellum.There were no significant deformities noticed on the surface topography of cryopreserved spermatozoa.Conclusions:Thus the results clearly documented that cryoprotectant 10%dimethyl sulfoxide affords better cryopreservation at 4℃for the spermatozoa of Cyprinus carpio.展开更多
Objective:To determine the effects of morphological characteristics of common carp(Cyprinus carpio)by fixation and preservation in 10%formalin.Methods:Fish samples were collected from the Research Center of Aquacultur...Objective:To determine the effects of morphological characteristics of common carp(Cyprinus carpio)by fixation and preservation in 10%formalin.Methods:Fish samples were collected from the Research Center of Aquaculture of Barabadi Fazli martyr,Gorgan University of Agricultural Sciences and Natural Resources,Gorgan,Iran.After initial assay on morphological characters such as total length,standard length and head length,samples were fixed in 10%formalin for 12 weeks.After this period,samples were taken away from the formalin and the morphological characteristics and evaluation of color features were assayed once again.Results:The results indicated that shrinkage was usual in all the specimens and changes in body and fins color were clear and their color was opaque.Formalin preservation is usually causing a reduction in the length of fishes.Conclusions:Different rates of change in body length and color of preserved sample are observed after a standard period of preservation in different preservatives and it can be suggested that the decrease of color intensity in fixed samples in formalin does not have inhibition effects in color identification keys.展开更多
基金supported by the National Key R&D Program of China (2019YFE0119000)National Natural Science Foundation of China (31872561)+1 种基金National Science Fund for Distinguished Young Scholars (32225049)Alliance of International Science Organizations (ANSO-CR-PP-2021-03)。
文摘Common carp are among the oldest domesticated fish in the world.As such,there are many food and ornamental carp strains with abundant phenotypic variations due to natural and artificial selection.Hebao red carp(HB,Cyprinus carpio wuyuanensis),an indigenous strain in China,is renowned for its unique body morphology and reddish skin.To reveal the genetic basis underlying the distinct skin color of HB,we constructed an improved highfidelity(HiFi) HB genome with good contiguity,completeness,and correctness.Genome structure comparison was conducted between HB and a representative wild strain,Yellow River carp(YR,C.carpio haematopterus),to identify structural variants and genes under positive selection.Signatures of artificial selection during domestication were identified in HB and YR populations,while phenotype mapping was performed in a segregating population generated by HB×YR crosses.Body color in HB was associated with regions with fixed mutations.The simultaneous mutation and superposition of a pair of homologous genes(mitfa) in chromosomes A06 and B06 conferred the reddish color in domesticated HB.Transcriptome analysis of common carp with different alleles of the mitfa mutation confirmed that gene duplication can buffer the deleterious effects of mutation in allotetraploids.This study provides new insights into genotype-phenotype associations in allotetraploid species and lays a foundation for future breeding of common carp.
基金supported by the Central Public-Interest Scientific Institution Basal Research Fund,CAFS(No.2020TD71).
文摘A precise and reliable analytical method of high performance liquid chromatography-tandem mass spectrometry(HPLCMS/MS)was developed to measure trace levels of enrofloxacin(ENR)and its major metabolite ciprofloxacin(CIP)in carp tissues.Optimized chromatographic separation was obtained on a Waters Xterra MS C_(18) reversed-phase column using gradient elution with methanol and 0.1%formic acid aqueous solution including 5mmolL^(-1) of ammonium acetate.The established method was applied to study the pharmacokinetics and distribution of ENR and CIP in tissues of carp following a single oral administration in feed at a dosage of 40mgkg^(-1) bw(body weight).Data were analyzed using DAS 2.0 dynamics software,and the experimental results suggest that ENR was rapidly absorbed and extensively distributed in carp tissues through systemic circulation,and the pharmacokinetic characteristics can be described with a two-compartment model.The elimination half-lives(t_(1/2β))from muscle,liver,gill,plasma and skin were 131,160,104,132 and 310 h,respectively.The areas under the drug concentration-time curves(AUC)for these tissues were 491,972,750,249 and 706hmgkg^(-1),respectively.The maximum concentration(C_(max))values were 13,29,37,9 and 5mgkg^(-1) with peak times(t_(max))of 8,4,4,2 and 4 h,respectively.Ciprofloxacin,the active metabolite of ENR,was also detected in carp tissues,indicating that only 1.54%of de-ethylation of ENR occurs in carp.At a water temperature of 18℃,the drug withdrawal time was determined to be no less than 24 d while the carp was fed at a single dosage of 40mgkg^(-1).
基金This work was supported by the National "973" Great Foundation Research Item of China (2002CB412303) and theNatural Science Foundation of Jiangsu Province (BK2004118).
文摘To measure the 96h-LC50 values of 32 substituted benzenes to the carp and to study the relationship between quantitative structure-activity and structural parameters of chemicals. Methods The acute toxicity values of 32 substituted benzenes to the carp were determined in a semistatic test. The energy of the lowest unoccupied molecular orbital, and the highest occupied molecular orbital, the dipole moment and the molecular weight of substituted benzenes were calculated by the quantum chemical method MOPAC6.0. Results The range of the toxicity of studied compounds was broad, and the most toxic compound was pentachlorophenol, while the least toxic compound was 4-methylaniline. By the stepwise regression analyses, a series of Quantitative structure-activity relationships (QSAR) equations were derived from all compounds and subclasses. The equation log1/LC50=0.759logP +2.222 (R2 (adj)=0.818) was found to fit well and the average predicted percentage error was 6.16%. Conclusion The toxicity of anilines and phenols to the carp could be modeled well by logP alone, whereas the toxicity of the halogenated benzenes and nitrobenznes not containing hydroxyl or amino group can be controlled by hydrophobic and electronic factors.
文摘Phenol, a ubiquitous component of industrial effluents, is a common pollutant of water resources and a serious threat to fish.The present work demonstrates that a significant amount of phenol is retained by various tissues of the common carp.Cyprinus carpio.and the snake-headed murrel.Channa punclatus.The rate of [^(14)C] phenol accumulation was higher in the carp than in the murrel.It is suggested that retention of phenol in the brain and ovary may seriously afiect the reproductive potential of the fish. 1990 Academic Press.Inc.
基金Supported by Agricultural Seed Development Project of Shandong ProvinceModern Agricultural Industrial Technology System Innovation Team of Shandong Province(SDAIT-14-011-03)
文摘To investigate the genetic composition of the carp (Cyprinus carpio) in Dongping Lake and to provide eytogenetie data for exploration, protection and appropriate utilization of the germplasm resources, karyotype of the carp was analyzed in this study. In detail, colehieine was given to the fish an intraperitoneal route 24 h after the intraperitoneal injection of phytohemagglutinin (PHA) solution to arrest cells in metaphase. The kidney cells were collected for the preparation of chromosome specimens, which were observed under a microscope. Ten cells having correct number of chromosomes and clear morphology were photographed and zoomed to measure the relative length, arm ratio, ann number (NF) and other parameters of chromosomes. The results showed that the chromosome number of C. earpio in Dongping Lake was (2n = 100) ; the karyotype formula was (2n =20m +38sm +20st +22t). The total number of chromosome arms (NF) was 158. By comparing the karyotype of Dongping Lake carp with that of other Cyprinus species, we concluded that Dongping Lake carp is more highly evolved among Cyprinus species.
文摘Isolated ovarian tissues from the common carp, Cyprinus carpio were incubated in vitro to obtain a discrete effect of four common toxicants of industrial origin, namely phenol, sulfide, mercuric chloride and cadmium chloride, on gonadotropin-induced alteration of nonesterified and esterified cholesterol and steroidogenic enzymes, △5-3β-HSD and 17β-HSD activity. Stage II ovarian tissue containing 30-40% mature oocytes were shown to be most responsive to gonadotropins in depleting only nonesterified cholesterol moiety and stimulating the activity of both. Safe doses of above mentioned toxicants when added separately to stage II ovarian tissue with oLH (1 μg/incubation) gonadotropin-induced depletion of nonesterified cholesterol and gonadotropin-induced stimulation of the activity of both enzymes was significantly inhibited. Esterified cholesterol remained almost unaltered. Findings clearly indicate the impairment of gonadotropin induced fish ovarian steroidogenesis by the four toxicants separately.
基金The author(D.HEMALATHA)wish to thank UGC,New Delhi for providing grant of Basic Science Research Fellowship(BSR)(No.F.7-24/2007).
文摘The genotoxic effects of quinalphos(QP)were evaluated in the erythrocytes of Cyprinus carpio using the comet,micronucleus,and nuclear abnormality assays.The 96 h LC_(50) value of quinalphos,estimated by static renewal system,was 10.9µL/L.On the basis of the LC_(50) value,two sublethal concentrations(1.09 and 2.18µL/L)of the compound were used together with cyclophosphamide(5 mg/L)as the positive control.The fish were exposed for 35 d,and sampling was done at the end of 7,14,21,28,and 35 d.Our results reveal a significant dose-dependent increase in the frequencies of DNA strand breaks,micronucleus,and nuclear abnormalities.Similar responses were also observed in fish exposed to the positive genotoxin cyclophosphamide.The collective findings demonstrated that quinalphos exerts a genotoxic effect on C.carpio.The results further confirmed that the comet,micronucleus,and nuclear abnormality assays are useful tools in determining the potential genotoxicity of pesticides towards aquatic organisms.
基金the Modern Agricultural Industrial Technology System of the Ministry of Agriculture and the Ministry of FinanceKey Scientific and Technological Planning Project of Jilin Province(20190301040NY).
文摘[Objectives]To study the effects of biological flocs on growth performance of Cyprinus carpio var.Furui No.2 and aquaculture water quality.[Methods]The comparative experiment method was adopted.An experimental group and a control group were set up,with 3 replicates in each group.The experimental group used beet molasses as the carbon source which was added once a week.The breed,quantity,and specifications of stocked fish in each group were exactly the same.The experiment lasted for 60 d.Fish growth indicators were measured every 7 d.After one week of the experiment,ammonia nitrogen and nitrite were monitored once a week,and dissolved oxygen was monitored once a day.[Results]The survival rate of fish,the average weight of,the weight gain rate,and the specific growth rate of the experimental group were significantly higher than those of the control group(P<0.05),and the feed conversion rate was significantly lower than that of the control group(P<0.05).One week after adding the carbon source in the experimental group,the ammonia nitrogen was lower than the control group,and after three weeks it was significantly lower than the control group(P<0.05);after adding the carbon source,the nitrite in the experimental group was lower than the control group,there was no significant difference in the first three weeks(P>0.05),and there was a significant difference after three weeks(P<0.05).The feed conversion rate of the experimental group was significantly lower than that of the control group(P<0.05).[Conclusions]Adding a carbon source can significantly reduce ammonia nitrogen,nitrite and other toxic and harmful substances in the aquaculture water,promote the growth of aquaculture fish and increase the feed utilization rate.
文摘Gonadal function in fish, CJprinus carpio was significantly affected by sublethal doses of mercuric chloride (HgCl2 ) and cadmium chloride (CdCl2 ) in chronic (45 days ) exposure.Parameters investigated were nonesterified (NE) and esterified (E) cholestcrol of ovary,liver and serum and ovarian 3β-Hydroxysteroid and 17β-Hydroxysteroid dehyrogenaseenzyme activity and serum and pituitary gonadotropin (GtH) levels. Both the pollutantswere able to reduce the hypothalamic extract (HE) or gonadotropin releasing hormone(GnRH) induced pituitary GtH release in vitro. Short term (96h) exposure of the fish tothe pollutants had no significant effect on the gonadal function. ln addition to thedeleterious effect of pollutants on the gonadal steroidogenesis and pituitary gonadotropin release, using [4-14C] cholesterol as a tracer it was found that for 45 days expeure, HgCl2'had an adverse effect on the transport of cholesterol from circulation to ovary.
文摘This study was conducted to evaluate the use of cyanobacteria Arthrospira platensis and cladoceran Daphnia magna biomass as complementary protein and lipid sources in diet supplementation for common carp (Cyprinus carpio L.) nursery. Three experimental diets containing A. platensis and D. magna meal at different concentrations were compared to the commercial (control) diet. Each experimental diet (ED) was set to contain D. magna and A. platensis meal at a specific combination: 2% and 5%, 2% and 7%, and 4% and 5% for ED1, ED2 and ED3, respectively. The protein and lipid content of the experimental diets ranged from 43.20% to 44.60% dry weight (DW) and 10.64% to 13.42% DW, respectively;while the protein and lipid content of the control diet were 43.00% DW and 6.72% DW, respectively. After 20 days of feeding period, ED3 group obtained the highest final body weight (BW) (58.18 ± 35.24 mg), total biomass (1936 ± 1625 mg), food conversion rate (1.34 ± 0.04), and specific growth rate (12.86 ± 0.03% BW day<sup>–1</sup>) among all treatment groups (P > 0.05);while ED1 group obtained the highest survival (75.5% ± 7.47%) among all treatment groups (P > 0.05). The total bacterial count and total pathogenic Aeromonas sp. in the culture water of the treatment groups (ranged from 2.00 to 2.65 × 10<sup>5 </sup>CFU mL<sup>–1</sup> and 3.50 to 4.12 × 10<sup>3</sup> CFU mL<sup>–1</sup>, respectively) were lower compared to the water of the control group fed (3.73 × 10<sup>5</sup> and 4.70 × 10<sup>3</sup> CFU mL<sup>–1</sup>, respectively). No significant differences in physicochemical water quality parameters were observed among treatments (P > 0.05). The current study suggests that the combination of A. platensis and D. magna biomass can be used as complementary protein and lipid sources in diet supplementation for common carp larvae and can result in a comparable fish growth, survival and feed utilization in common carp culture at the nursery phase.
基金This work was supported by National Natural Science Foundation of China(31902361,21906046)the Key Technology Research Project of Henan Province(202102110106).
文摘Polybrominated diphenyl ether has become a widespread environmental pollutant in aquatic environments.BDE-209,a prominent PBDEs found in the environment and wildlife,has been proven to cause oxidative stress,immune damage and endocrine system disorders in fish.However,the effects of BDE-209 on the intestinal health of fish remain unclear.This research aimed to determine the effects of BDE-209 on the growth performance,digestive enzymes activities and intestinal microbiota of common carp.After 60-day exposure,the growth performance of common carp were remarkably decreased(P<0.05).Moreover,the levels of protease,amylase and lipase activities remarkably decreased by BDE-209 exposure(P<0.05).In addition,metagenomics analysis revealed that the alpha-diversity values of intestinal microbiota were significantly decreased in BDE-209 exposure groups(P<0.05).The results also indicated that BDE-209 exposure could alter the intestinal microbial compositions.At the phylum level,the proportion of Fusobacteria was markedly reduced(P<0.05),while the proportions of Bacteroidetes and Firmicutes were significantly increased(P<0.05)along with the increasing concentrations of BDE-209.At the genus level,the proportion of Shewanella was higher(P<0.05)in BDE-209 exposure groups than in control group,while the abundance of Cetobacterium showed an opposite trend.Furthermore,the proportions of some aquatic probiotics(such as Bacillus,Rhodopseudomonas and Lactobacillus)were significantly enriched(P<0.05)in control group,and the content of short-chain fatty acid producing bacterium(such as Faecalibacterium,Bacteroides and Clostridium)were decreased along with the increasing BDE-209 concentrations,but no significant differences were found among the four groups(P>0.05).Overall,exposure to BDE-209 could alter the intestinal microbiota of common carp,which may affect their growth and threaten their health.
基金The authors would like to express their gratitudes to the Institute for Research and Community Service,Universitas Brawijaya,Indonesia through the“Doktor Mengabdi 2018"[grant DIPA number:DIPA-042.01.2.400919,2018].
文摘A poor environment increases fish’s susceptibility to myxosporean infection that can cause the death of larval fish,especially for koi fish(Cyprinus carpio).This study aimed to determine the effect of probiotics,local anti-parasitic drugs(kutuklin),and the chemical compound diflubenzuron treatments on the koi immune response.This study used PCR with specific primer 18S SSU rDNA and DNA sequencing to detect Myxobulus phylogenetic.The treatments were divided into 5 groups:Treatment(A)(healthy koi without treatment),(B)(infected koi without treatment),(C)(infected koi with 0.55 mL/30 L probiotics),(D)(infected koi with 1μL/g of feed kutuklin),and(E)(infected koi with 0.02 mg/5 L dimilin).Myxospore has observed with Scanning Electron Microscopy(SEM)and 4′,6-diamidino-2-phenylindole(DAPI)fluorescence staining.The histological analysis using semi-quantitative scoring methods,and flow cytometry was conducted to analyse the immune response of Cluster of differentiation 4(CD4^(+)),Cluster of differentiation 8(CD8^(+)),Tumor Necrosis Factor-alpha(TNF-α),Interferon gamma(IFN-γ)cells in the gills.Results show that the histological analysis indicated edema,hyperplasia,lamella fusion,congestion,and hypertrophy lesions in infected koi.Treatment with probiotics shows the lowest damage(30.6%).The immune responses of CD4^(+)and CD8^(+)cells to dimilin treatment were 10.54%and 16.86%,respectively.The largest TNF-αand IFN-γresponse were for the kutuklin treatment(29.26%)and probiotics treatment(8.23%).
基金supported by the National Natural Science Foundation of China(Nos.31202002,31200918)Jiangsu Science and Technology Department(No.BK2012535)the Central Public-Interest Scientific Institution Basal Research Fund(No.2014A08YQ01)
文摘To evaluate the protective effects of Glycyrrhiza polysaccharide(GPS) against 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD)-induced hepatotoxicity in Jian carp,the fish were fed diets containing GPS at doses of 0.1,0.5 and 1.0 g/kg for 60 days before an intraperitoneal injection of 0.6 μg/kg TCDD at a volume of 0.05 mL/10 g body weight.At 72 hr post-injection,blood and liver samples were taken for biochemical analysis and the fish liver samples were used for the preparation of pathological slices.The results showed that increases in alanine aminotransferase(GPT),aspartate aminotransferase(GOT),lactate dehydrogenase(LDH),and alkaline phosphatase(AKP) in serum induced by TCDD were significantly inhibited by pre-treatment with 1.0 g/kg GPS.Following the 1.0 g/kg GPS pre-treatment,total protein(TP),albumin(Alb),catalase(CAT),glutathione peroxidase(GPx),total antioxidant capacity(T-AOC) and superoxide dismutase(SOD) activities in liver tissue increased significantly,malondialdehyde(MDA) formation(P &lt; 0.05 or P &lt; 0.01) was significantly inhibited,and the expression of cytochrome P4501A(CYP1A),aryl hydrocarbon receptor 2(AHR2) and aryl hydrocarbon receptor nuclear translocator 2(ARNT2) mRNA(P &lt; 0.05) was significantly enhanced.Histological observations on fish liver were obtained by preparing paraffin tissue sections via HE staining,and the results showed that histological changes were obviously reduced by 0.5 and 1.0 g/kg GPS.GPS significantly reduced liver tissue damage caused by TCDD.Overall,these results proved the hepatoprotective effect of GPS in protecting against fish liver injury induced by TCDD,and supported the use of GPS(1.0 g/kg) as a hepatoprotective and antioxidant agent in fish.
基金supported by the Natural Science Foundation of China(Grant No.30700622)the Shanghai Education Committee Project(No.07ZZ136)the Shanghai Leading Academic Discipline Project(No.Y1101).
文摘The common carp(Cyrpinus carpio L.)is the oldest cultured and the most domesticated fish species,as well as one of the most important freshwater fishes in the world.However,scientific studies on evaluating the growth-related quantitative traits in this fish are limited.Heritability,the most important parameter in selective breeding programs,was extensively studied for the growth-related traits.The values varied widely among the experiments and methods used because of the existence of common environmental,dominance and maternal effects.However,correlations in phenotypic and genetic levels first evaluated several years ago were limited.On the other hand,heterosis was widely reported and easily obtained for growth-related traits in the common carp.Meanwhile,genotype environment interaction and prediction of breeding values have been studied recently,and are very important in conducting selective breeding programs.The developmental quantitative genetics of growth-related traits was first analyzed in the common carp for reasonable selection during ontogeny.It is expected that genetic improvement will be achieved by carrying out direct selective breeding in the common carp.
基金Supported by the Gorgan University of Agriculture Science&Natural Resource(Grant No.6177515-5).
文摘Objective:To determine acute toxicity of abamectin(abamectin used for agricultural fields and also is a common acaridae used in farms)to common carp(Cyprinus carpio).Methods:In this research,common carps were exposed to abamectin for 96 h.LC_(50) values of 24 h,48 h,72 h and 96 h were attained by probit analysis software SPSS Version 16.Fish were exposed to different concentrations(1,2,3,6,12 and 15 mg/L)of abamectin for 96 h and physicochemical properties of water used for these experiments were stable and every mortality was recorded daily.Results:The 96 h LC_(50)of abamectin for Cyprinus carpio was 1.243 mg/L.Conclusions:Eventually toxicity values indicated that abamectin has same toxicity in studied other specie and we can state lower value of LC_(50)for studied specie in compare with most species.
基金This study was co-funded by the National Natural Science Foundation of China (Grant No. 50979006), Projects of Hebei Science and Technology Department (No. 12276708D), Projects of Hebei Education Department (No. ZH2011119), Natural Science Foundation of Hebei Province (No. D2010000867), the Doctor Development Foundation Project in Hebei University of Science and Technology (No. 000446), and the Key Discipline Foundation in Hebei Province.
文摘This study was conducted to evaluate the toxicological response ofp-nitrotoluene and p-nitroaniline to the key fish species, Cyprinus carpio. A freshwater fish bioassay based on the 96 h LC50 was used to estimate the single and joint toxicity of the two chemicals. The toxicity of p-nitrotoluene was greater than that of pnitroaniline based on 96 h LCso values of 40.74 mg.L^-1 and 48.99mg.L^-1, respectively. Both compounds had moderate toxicity toward Cyprinus carpio, and this toxicity increased with the exposure duration and con- centration. Binary mixtures of the compounds were more toxic than the individual compounds at 96 h, and they acted upon partial addition. When the exposure time was longer, the toxicity increased for mixtures of compounds with the same concentration or toxicity. The results of this study suggest that exposure to a combination of these chemicals would result in a higher environmental risk in aquatic systems than exposure to either compound alone. Further research is needed to investigate the combined effects and sublethal toxicity of p-nitrotoluene and p- nitroaniline, since they are both still used in China.
文摘Objective:To investigate the effects of cadmium(Cd)and copper(Cu)on sialic acid levels of brain and blood tissues of Cyprinus carpio.Methods:Adult carps were exposed to 0.1,0.5 mg/L Cu,0.1,0.5 and 1.0 mg/L Cd and 0.1 mg/L Cu+0.1 mg/L Cd under static experiment conditions for 1 week.At the end of exposure period,heavy metal accumulations and sialic acid levels in blood and brain tissues of the test animals were analyzed.Results:Cu and Cd accumulated in tissues in a dramatically increasing dose-dependent manner.Sialic acids level of the fish exposed to 0.1,0.5 and 1.0 mg/L Cu and Cd and control grups for 1 week were 0.834,1.427,0.672,0.934,2.968,4.714 mg/mL respectively.The results also showed that Cu has an antagonistic effect on tissue sialic acid level.Conclusions:We propose that Cd and Cu make a complex with sialic acids of membranes in the tissues researched.This complex between metal ions and sialic acid migth account for the cellular toxicity based on Cu and Cd.
基金Supported by DST,Government of India(Grant No.SR/SO/As-03/2003)and UGC-DRS-II.
文摘Objective:To investigate the effects of various extenders containing different cryoprotectants on post-thaw viability,motility and scanning electron microscopic study of frozen spermatozoa from carp.Methods:cryopreservation has been achieved using extender and cryoprotectants like dimethylacetamide After screening a variety of cryoprotectants and extenders,a protocol for the 5%-20%at appropriate dilution ratio(1:10).For all experimental tests,the motility and viability percentage of spermatozoa were examined after storage of 5 d at 4℃.Results:The maximum motility of 75%has been observed with 10%dimethyl sulfoxide.Scanning electron microscopic studies on normal and cryopreserved spermatozoa showed prominent head,middle piece and different segments of flagellum.There were no significant deformities noticed on the surface topography of cryopreserved spermatozoa.Conclusions:Thus the results clearly documented that cryoprotectant 10%dimethyl sulfoxide affords better cryopreservation at 4℃for the spermatozoa of Cyprinus carpio.
基金Supported by Gorgan University of Agricultural Sciences and Natural Resources,Gorgan,Iran(Grants No.5039480687).
文摘Objective:To determine the effects of morphological characteristics of common carp(Cyprinus carpio)by fixation and preservation in 10%formalin.Methods:Fish samples were collected from the Research Center of Aquaculture of Barabadi Fazli martyr,Gorgan University of Agricultural Sciences and Natural Resources,Gorgan,Iran.After initial assay on morphological characters such as total length,standard length and head length,samples were fixed in 10%formalin for 12 weeks.After this period,samples were taken away from the formalin and the morphological characteristics and evaluation of color features were assayed once again.Results:The results indicated that shrinkage was usual in all the specimens and changes in body and fins color were clear and their color was opaque.Formalin preservation is usually causing a reduction in the length of fishes.Conclusions:Different rates of change in body length and color of preserved sample are observed after a standard period of preservation in different preservatives and it can be suggested that the decrease of color intensity in fixed samples in formalin does not have inhibition effects in color identification keys.