为了进一步探讨大麝鼩(Crocidura lasiura)的亚种分化问题,在中国东北地区采集大麝鼩标本13号,结合已报道的大麝鼩形态学数据和Cyt b基因序列一并进行分析。形态学结果显示:在绝大多数个体中,分布于中国东北—俄罗斯远东的大麝鼩体型大...为了进一步探讨大麝鼩(Crocidura lasiura)的亚种分化问题,在中国东北地区采集大麝鼩标本13号,结合已报道的大麝鼩形态学数据和Cyt b基因序列一并进行分析。形态学结果显示:在绝大多数个体中,分布于中国东北—俄罗斯远东的大麝鼩体型大于分布于韩国的个体(体长:> 82 mm vs.<82 mm,尾长:> 39 mm vs.<39 mm,颅基长:> 20 mm vs.<20 mm);中国东北—俄罗斯远东种群体背毛色更黑,韩国种群体背毛色更灰;中国东部种群尾较长,绝大多数超过体长之半;而分布于中国东北—俄罗斯远东、韩国的大麝鼩种群尾长绝大多数不及体长之半。基于Cyt b基因的遗传距离、系统发育关系和中介网络分析,都显示大麝鼩样本可分为3个地理谱系:中国东北—俄罗斯远东谱系、韩国谱系、中国东部谱系。研究结果支持大麝鼩分为3个亚种:东北亚种(C. l. lasiura)分布于中国东北和俄罗斯远东,韩国亚种(C. l. thomasi)分布于韩国,华东亚种(C. l. campus-lincolnensis)分布于中国东部。展开更多
Cyt b gene of North Sulawesi Tarsius sp., T. tumpara, T. sangirensis and T. tarsier (T. spectrum) had been partially sequenced. The homologous sequence of three groups had been compared to describe the phylogenetic po...Cyt b gene of North Sulawesi Tarsius sp., T. tumpara, T. sangirensis and T. tarsier (T. spectrum) had been partially sequenced. The homologous sequence of three groups had been compared to describe the phylogenetic position among them, as well as several other species accessed from the Genbank. Total DNA extracted from the muscular tissue had been obtained through tail cut sampling using the innuPREP DNA micro kit, and amplified using a pair of universal primer, L14841 and H15149. The size of the cyt b gene sequence amplified was 307 bp long. Sequence aligned using CLUSTAL-X program and diversity analysis were done using version 5.2.2. MEGA5 program. Genetic distance had been calculated by Tamura 3 parameter method and phylogenetic tree had been built using Neighbor-Joining and Maximum Likelihood methods. Genetic distance based on cyt b gene nucleotide was found from 0 to 0.240 with an average of 0.080. The phylogenetic tree constructed by Neighbor Joining and Maximum Likelihood methods indicated that T. tarsier, T. sangirensis and T. tumpara were closely related with Tarsius tarsier-complex, and distantly related with Cephalopachus bancanus and Carlito syrichta. The genetic distance and the phylogenetic tree had been constructed on the base of partial cyt b gene sequence of T. tarsier, T. sangirensis, T. tumpara and 5 other tarsier species and their accession. Those results are consistent with taxonomy based on morphology and vocal acoustic form.展开更多
文摘为了进一步探讨大麝鼩(Crocidura lasiura)的亚种分化问题,在中国东北地区采集大麝鼩标本13号,结合已报道的大麝鼩形态学数据和Cyt b基因序列一并进行分析。形态学结果显示:在绝大多数个体中,分布于中国东北—俄罗斯远东的大麝鼩体型大于分布于韩国的个体(体长:> 82 mm vs.<82 mm,尾长:> 39 mm vs.<39 mm,颅基长:> 20 mm vs.<20 mm);中国东北—俄罗斯远东种群体背毛色更黑,韩国种群体背毛色更灰;中国东部种群尾较长,绝大多数超过体长之半;而分布于中国东北—俄罗斯远东、韩国的大麝鼩种群尾长绝大多数不及体长之半。基于Cyt b基因的遗传距离、系统发育关系和中介网络分析,都显示大麝鼩样本可分为3个地理谱系:中国东北—俄罗斯远东谱系、韩国谱系、中国东部谱系。研究结果支持大麝鼩分为3个亚种:东北亚种(C. l. lasiura)分布于中国东北和俄罗斯远东,韩国亚种(C. l. thomasi)分布于韩国,华东亚种(C. l. campus-lincolnensis)分布于中国东部。
文摘Cyt b gene of North Sulawesi Tarsius sp., T. tumpara, T. sangirensis and T. tarsier (T. spectrum) had been partially sequenced. The homologous sequence of three groups had been compared to describe the phylogenetic position among them, as well as several other species accessed from the Genbank. Total DNA extracted from the muscular tissue had been obtained through tail cut sampling using the innuPREP DNA micro kit, and amplified using a pair of universal primer, L14841 and H15149. The size of the cyt b gene sequence amplified was 307 bp long. Sequence aligned using CLUSTAL-X program and diversity analysis were done using version 5.2.2. MEGA5 program. Genetic distance had been calculated by Tamura 3 parameter method and phylogenetic tree had been built using Neighbor-Joining and Maximum Likelihood methods. Genetic distance based on cyt b gene nucleotide was found from 0 to 0.240 with an average of 0.080. The phylogenetic tree constructed by Neighbor Joining and Maximum Likelihood methods indicated that T. tarsier, T. sangirensis and T. tumpara were closely related with Tarsius tarsier-complex, and distantly related with Cephalopachus bancanus and Carlito syrichta. The genetic distance and the phylogenetic tree had been constructed on the base of partial cyt b gene sequence of T. tarsier, T. sangirensis, T. tumpara and 5 other tarsier species and their accession. Those results are consistent with taxonomy based on morphology and vocal acoustic form.
