期刊文献+
共找到1,412篇文章
< 1 2 71 >
每页显示 20 50 100
Natural variation in the cytochrome c oxidase subunit 5B OsCOX5B regulates seed vigor by altering energy production in rice
1
作者 Chengwei Huang Zhijuan Ji +7 位作者 Qianqian Huang Liling Peng Wenwen Li Dandan Wang Zepeng Wu Jia Zhao Yongqi He Zhoufei Wang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第9期2898-2910,共13页
Seed vigor is a crucial trait for the direct seeding of rice.Here we examined the genetic regulation of seed vigor traits in rice,including germination index(GI)and germination potential(GP),using a genome-wide associ... Seed vigor is a crucial trait for the direct seeding of rice.Here we examined the genetic regulation of seed vigor traits in rice,including germination index(GI)and germination potential(GP),using a genome-wide association study approach.One major quantitative trait locus,qGI6/qGP6,was identified simultaneously for both GI and GP.The candidate gene encoding the cytochrome c oxidase subunit 5B(OsCOX5B)was validated for qGI6/qGP6.The disruption of OsCOX5B caused the vigor traits to be significantly lower in Oscox5b mutants than in the japonica Nipponbare wild type(WT).Gene co-expression analysis revealed that OsCOX5B influences seed vigor mainly by modulating the tricarboxylic acid cycle process.The glucose levels were significantly higher while the pyruvic acid and adenosine triphosphate levels were significantly lower in Oscox5b mutants than in WT during seed germination.The elite haplotype of OsCOX5B facilitates seed vigor by increasing its expression during seed germination.Thus,we propose that OsCOX5B is a potential target for the breeding of rice varieties with enhanced seed vigor for direct seeding. 展开更多
关键词 cytochrome c oxidase natural variation RIcE seed vigor
下载PDF
二乙酰吗啡致大鼠小脑颗粒神经元细胞凋亡过程中C-Jun、Cytc和Caspase-9的作用 被引量:6
2
作者 苏丽萍 路子扬 +5 位作者 刘丽 张巍 苏天园 胡夏韵 蒲红伟 韩登峰 《中国组织工程研究》 CAS 北大核心 2021年第25期3943-3948,共6页
背景:二乙酰吗啡药物可导致神经元损伤,但是目前二乙酰吗啡是否诱导神经元细胞凋亡,C-jun、Cytc和Caspase-9因子是否参与此过程尚未见报道。目的:探讨C-jun、Cytc、Caspase-9是否参与二乙酰吗啡诱导神经元细胞凋亡过程。方法:将SD乳鼠... 背景:二乙酰吗啡药物可导致神经元损伤,但是目前二乙酰吗啡是否诱导神经元细胞凋亡,C-jun、Cytc和Caspase-9因子是否参与此过程尚未见报道。目的:探讨C-jun、Cytc、Caspase-9是否参与二乙酰吗啡诱导神经元细胞凋亡过程。方法:将SD乳鼠小脑颗粒神经元细胞体外培养7 d,采用不同质量浓度二乙酰吗啡(0,10,40,80,100,120 mg/L)干预神经元细胞24 h,在倒置荧光显微镜下观察神经元细胞形态改变,流式细胞仪检测细胞凋亡率。采用100 mg/L二乙酰吗啡和20μmol/L JNK抑制剂SP600125作用于细胞24 h,通过免疫荧光和蛋白印迹方法检测C-jun、Cytc和Caspase-9蛋白的表达。结果与结论:①在不同质量浓度二乙酰吗啡干预下,随着给药质量浓度的增加,神经元细胞胞体萎缩、亮度降低,部分呈灰黑色,细胞碎裂,神经元网状结构不同程度的消失,细胞改变数量逐渐增加;②随着给药质量浓度的增加,形态改变的细胞数量明显增多(P <0.01),细胞凋亡率也显著增高(P <0.01);③与对照组相比,100 mg/L二乙酰吗啡作用神经元细胞时,二乙酰吗啡组C-jun、Cytc和Caspase-9蛋白呈高表达,差异有显著性意义(P <0.05);与二乙酰吗啡组相比,二乙酰吗啡+SP600125组C-jun、Cytc和Caspase-9蛋白表达明显下调,差异有显著性意义(P <0.05);④结果表明,二乙酰吗啡可诱导小脑颗粒神经元细胞凋亡,C-jun、Cytc和Caspase-9参与了二乙酰吗啡致神经元细胞凋亡的过程。 展开更多
关键词 二乙酰吗啡 小脑 神经元 c-JUN cytc cASPASE-9 凋亡
下载PDF
低硒大鼠心肌线粒体CytC含量的动态变化及其在心肌细胞凋亡中的作用 被引量:9
3
作者 潘晓青 魏瑾 +4 位作者 林琳 张明 单虎 闫蕊 贺乐 《西安交通大学学报(医学版)》 CAS CSCD 北大核心 2013年第2期177-180,共4页
目的检测低硒大鼠心肌线粒体中细胞色素C(CytC)的蛋白含量及心肌细胞凋亡情况,探讨低硒时CytC在线粒体介导的细胞凋亡中的作用机制。方法构建低硒大鼠模型,于喂养20、30、40周时提取心肌线粒体,Western Blot法检测线粒体中CytC的蛋白表... 目的检测低硒大鼠心肌线粒体中细胞色素C(CytC)的蛋白含量及心肌细胞凋亡情况,探讨低硒时CytC在线粒体介导的细胞凋亡中的作用机制。方法构建低硒大鼠模型,于喂养20、30、40周时提取心肌线粒体,Western Blot法检测线粒体中CytC的蛋白表达;免疫组织化学技术(SP法)对死亡结构域蛋白FADD进行原位染色,统计阳性细胞指数,并对CytC与心肌细胞凋亡指数进行相关性分析。结果低硒组CytC的表达与相应时段的对照组相比均降低,且随低硒喂养时间延长,降低更为明显,差异均有统计学意义(P<0.05);各时间对照组间心肌线粒体中CytC的表达无明显差异;免疫组化染色显示,随着喂养时间的延长,低硒组大鼠心肌细胞凋亡加重;相关性分析显示,线粒体内CytC表达与心肌细胞凋亡指数呈负相关(rs=-0.858,P<0.01)。结论低硒可影响大鼠心肌线粒体中CytC蛋白的表达;CytC表达与心肌细胞凋亡指数具有相关性,提示CytC在线粒体介导的心肌细胞凋亡中起重要作用。 展开更多
关键词 心肌线粒体 细胞色素c 凋亡 低硒 心肌细胞凋亡
下载PDF
黄芪注射液对早期大鼠脑出血灶周围神经元CytC和线粒体ATPase的影响 被引量:8
4
作者 孙征 王登科 +6 位作者 张海宇 刘海洋 李强 韩金利 张莲香 王效军 秦毅 《宁夏医科大学学报》 2011年第5期407-410,共4页
目的探讨黄芪注射液对大鼠脑出血灶周围凋亡神经元线粒体Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶和细胞色素C的影响。方法将32只雄性SD大鼠随机分为黄芪治疗组、出血模型组和正常对照组,其中黄芪治疗组又分为出血后6h治疗组和24h治疗组。选用胶... 目的探讨黄芪注射液对大鼠脑出血灶周围凋亡神经元线粒体Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶和细胞色素C的影响。方法将32只雄性SD大鼠随机分为黄芪治疗组、出血模型组和正常对照组,其中黄芪治疗组又分为出血后6h治疗组和24h治疗组。选用胶原酶Ⅶ-肝素液注射到大鼠尾状核建立脑出血动物模型,48h后测定各组大鼠脑出血灶周围神经元线粒体Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶的活性,以及神经元内细胞色素C的改变。结果与正常组比较,出血模型组细胞色素C的表达明显增加(P<0.01),ATP酶的活性均明显降低(P<0.01)。与出血模型组比较,6h治疗组及24h治疗组的细胞色素C表达均明显减少(P<0.05),ATP酶的活性均明显增加(P<0.05);6h治疗组与24h治疗组比较,细胞色素C的表达差异无统计学意义(P>0.05),ATP酶的活性6h组高于24h组(P<0.05)。结论黄芪注射液进行干预可减少脑出血灶早期神经元细胞内细胞色素C的释放及提高Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶的活性,从而减轻脑出血灶区神经细胞的凋亡,并且出血6h用药较出血24h后用药效果更好。 展开更多
关键词 黄芪注射液 脑出血 细胞凋亡 细胞色素c ATP酶
下载PDF
α-细辛醚诱导的Eca-109细胞中cytC、bax的表达 被引量:4
5
作者 张妍 韩倩倩 朱艳琴 《郑州大学学报(医学版)》 CAS 北大核心 2017年第3期251-254,共4页
目的:观察α-细辛醚对食管癌Eca-109细胞凋亡及cytC、bax表达的影响,探讨其诱导细胞凋亡的机制。方法:体外培养人食管癌Eca-109细胞,以15 mmol/L 5-氟尿嘧啶(5-FU)和25、50、100 mg/L的α-细辛醚干预48 h后,DAPI染色、倒置荧光显微镜下... 目的:观察α-细辛醚对食管癌Eca-109细胞凋亡及cytC、bax表达的影响,探讨其诱导细胞凋亡的机制。方法:体外培养人食管癌Eca-109细胞,以15 mmol/L 5-氟尿嘧啶(5-FU)和25、50、100 mg/L的α-细辛醚干预48 h后,DAPI染色、倒置荧光显微镜下观察细胞形态,MTT检测细胞增殖情况,Annexin V-PI法检测细胞凋亡率,分别采用Western blot和实时荧光定量PCR法检测cytC、bax mRNA及蛋白的表达。以无干预细胞作空白对照。结果:与空白对照组比较,α-细辛醚组和5-FU组细胞的增殖能力降低,凋亡率升高(P<0.05),细胞中cytC、bax蛋白及mRNA相对表达量均升高(P<0.05)。结论:α-细辛醚可能通过上调线粒体通路cytC、bax的表达,抑制Eca-109细胞增殖,促进其凋亡。 展开更多
关键词 Α-细辛醚 细胞色素c BAX EcA-109细胞
下载PDF
猪心中提取和纯化辅酶Q_(10)(联产CytC) 被引量:15
6
作者 袁艺 《安徽农业大学学报》 CAS CSCD 1997年第2期200-203,共4页
辅酶Q10和细胞色素C是促生物氧化酶类生化药物,本试验从猪心中提取和纯化辅酶Q10,并联产细胞色素C。猪心以酸性水液粗提,细胞色素C经人造沸石吸附,洗脱,盐析,三氯乙酸沉淀,弱酸性阳离子吸附,真空干燥得225mgCy... 辅酶Q10和细胞色素C是促生物氧化酶类生化药物,本试验从猪心中提取和纯化辅酶Q10,并联产细胞色素C。猪心以酸性水液粗提,细胞色素C经人造沸石吸附,洗脱,盐析,三氯乙酸沉淀,弱酸性阳离子吸附,真空干燥得225mgCytC/kg猪心,纯度79%。提取后的猪心渣,用醇碱皂化法,经硅胶柱层析,无水乙醇结晶、重结晶,每千克猪心渣可提取辅酶Q1075mg,含量为95.3%。 展开更多
关键词 辅酶Q10 细胞色素c 化学提取 纯化 猪心 心脏
下载PDF
线粒体CytC的释放机制及其在细胞凋亡中的作用 被引量:28
7
作者 李宏 冯有胜 《生物信息学》 2010年第3期210-213,共4页
描述了细胞色素C(CytC)从线粒体释放的机制及其在细胞凋亡中的作用,主要阐述了细胞凋亡信号转导途径中的线粒体-CytC途径及AIF途径;CytC在细胞凋亡中的作用及介导细胞凋亡的方式。探讨了线粒体CytC的泄漏机制,对非特异性释放模式(MPTP... 描述了细胞色素C(CytC)从线粒体释放的机制及其在细胞凋亡中的作用,主要阐述了细胞凋亡信号转导途径中的线粒体-CytC途径及AIF途径;CytC在细胞凋亡中的作用及介导细胞凋亡的方式。探讨了线粒体CytC的泄漏机制,对非特异性释放模式(MPTP假说和线粒体膨胀假说)、特异性释放模式(专一性通道假说)进行了阐述,最后分析了研究CytC与细胞凋亡分子机制的意义并提出细胞凋亡分子机制中一些未完全研究清楚的问题。 展开更多
关键词 Apaf-2/cytc 细胞凋亡途径 线粒体 泄漏
下载PDF
甜茶素对棕榈酸诱导INS-1细胞超微结构及CytC易位表达的保护作用 被引量:4
8
作者 郑华 计可为 +2 位作者 周莲清 孟春梅 苏志恒 《中国民族民间医药》 2015年第24期16-18,26,共4页
目的:探索甜茶素对棕榈酸诱导INS-1细胞损伤的保护机制。方法:MTT法检测正常对照组、棕榈酸模型组、不同浓度甜茶素组细胞存活率,透射电镜观察各组细胞超微结构,免疫电镜观察各组细胞色素C的易位表达。结果:不同浓度甜茶素对棕榈酸诱导... 目的:探索甜茶素对棕榈酸诱导INS-1细胞损伤的保护机制。方法:MTT法检测正常对照组、棕榈酸模型组、不同浓度甜茶素组细胞存活率,透射电镜观察各组细胞超微结构,免疫电镜观察各组细胞色素C的易位表达。结果:不同浓度甜茶素对棕榈酸诱导的INS-1细胞具有保护作用,超微结构保存较好;模型组细胞凋亡增多且超微结构损伤严重,Cyt C蛋白从线粒体释放入细胞质。结论:甜茶素可以提高棕榈酸诱导INS-1损伤的细胞存活率,抑制细胞凋亡的发生,其机制可能与抑制棕榈酸引起的氧化应激和阻碍Cyt C从线粒体转位入细胞质,激活细胞凋亡蛋白酶级联反应有关。 展开更多
关键词 甜茶素 棕榈酸 INS-1细胞 超微结构 细胞色素c
下载PDF
丙泊酚对大鼠脑缺血损伤后凋亡神经元线粒体CytC和ATPase的影响 被引量:1
9
作者 李秀华 刘雨清 孙征 《医学信息(医学与计算机应用)》 2014年第23期66-67,共2页
目的:探讨丙泊酚对大鼠脑缺血再灌注损伤后海马区凋亡神经元线粒体Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶和细胞色素C(CytC)的影响。方法36只雄性SD大鼠随机分为假手术组,模型组和丙泊酚组(n=12),复制大鼠全脑缺血再灌注模型,采用酶联免疫吸附... 目的:探讨丙泊酚对大鼠脑缺血再灌注损伤后海马区凋亡神经元线粒体Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶和细胞色素C(CytC)的影响。方法36只雄性SD大鼠随机分为假手术组,模型组和丙泊酚组(n=12),复制大鼠全脑缺血再灌注模型,采用酶联免疫吸附法检测大鼠脑海马区CytC表达;采用比色法测定大鼠脑海马区凋亡神经元线粒体ATPase的改变。结果与假手术组比较,模型组和丙泊酚组CytC的表达均增加(<0.05),ATP酶的活性均降低(<0.05);丙泊酚组大鼠脑组织海马区CytC的表达低于模型组(<0.05),ATP酶的活性高于模型组(<0.05)。结论丙泊酚的神经保护与改善脑缺血损伤后海马区凋亡神经元CytC的释放和ATPase的活性有关。 展开更多
关键词 丙泊酚 脑缺血 细胞凋亡 细胞色素c ATP酶
下载PDF
基于线粒体COⅠ基因序列的梭鲈野生群体遗传结构 被引量:1
10
作者 鲁翠云 孙志鹏 +4 位作者 曹顶臣 耿龙武 那荣滨 吴学工 郑先虎 《水产学报》 CSCD 北大核心 2024年第1期82-92,共11页
为了解梭鲈种群的遗传结构,实验利用线粒体细胞色素c氧化酶Ⅰ亚基(COⅠ)基因部分序列分析了中国6个和中亚2个群体的遗传差异,并与欧洲群体的单倍型序列进行了比较。结果在640 bp的COⅠ基因序列中检测到5个变异位点,定义了7种单倍型,发现... 为了解梭鲈种群的遗传结构,实验利用线粒体细胞色素c氧化酶Ⅰ亚基(COⅠ)基因部分序列分析了中国6个和中亚2个群体的遗传差异,并与欧洲群体的单倍型序列进行了比较。结果在640 bp的COⅠ基因序列中检测到5个变异位点,定义了7种单倍型,发现Hap1为8个梭鲈群体的共享单倍型,且与欧洲群体的HapA相同,在中国群体所占比例(93.36%)高于中亚群体(72.58%)和欧洲群体(53.85%);Hap2和Hap3是中国群体的特异单倍型,而Hap4~Hap7为中亚群体的特异单倍型。单倍型序列的聚类图和网络图均显示Hap1/A为梭鲈群体的原始单倍型,中国和中亚群体的特异单倍型相对于原始单倍型仅有1~2个位点的变异,属于Hap1/A的亚型,与欧洲群体的特异单倍型具有较大的差异。每个群体检测到1~4种单倍型,斋桑湖(ZS)群体单倍型最多,而中国的腾格里湖(NX)、兴凯湖(XK)和鸭绿江(YJ)群体仅有1个单倍型(Hap1);塔什干(TS)群体的单倍型多样性(Hd)和核苷酸多样性(π)最高(Hd=0.514±0.069;π=0.00079±0.00011),其次是ZS群体,而中国梭鲈群体的多样性参数较低。AMOVA分析结果显示,梭鲈群体间遗传变异占20.74%,群体间遗传分化程度较高(0.15≤F_(st)=0.20736<0.25),TS群体与ZS群体和中国群体间的遗传分化极大(F_(st)>0.25),中国群体中仅黑河(HH)群体与其他群体的遗传分化较大,而中国其他5个群体间无遗传分化。基于群体间遗传距离的系统进化树显示,来自中国的6个梭鲈群体与哈萨克斯坦的ZS群体聚为一支,而乌兹别克斯坦的TS群体独立为一支。研究结果为梭鲈群体的繁殖及放流管理提供了参考。 展开更多
关键词 梭鲈 线粒体cOⅠ基因 野生群体 遗传结构
下载PDF
细胞色素c(Cytc)诱导烟草悬浮细胞(BY-2)凋亡 被引量:4
11
作者 王庆华 邓文君 李艳红 《植物生理学通讯》 CSCD 北大核心 2004年第6期666-668,共3页
用不同浓度细胞色素c(Cyt c)诱导继代时间不同的烟草悬浮细胞48 h后观察形态学特征的结果表明,继代培养10 和13 d的细胞均在10mmol·L-1Cyt c时出现最高的细胞凋亡率,而继代5 d的细胞在Cyt c浓度为12.5mmol·L-1时细胞凋 亡的... 用不同浓度细胞色素c(Cyt c)诱导继代时间不同的烟草悬浮细胞48 h后观察形态学特征的结果表明,继代培养10 和13 d的细胞均在10mmol·L-1Cyt c时出现最高的细胞凋亡率,而继代5 d的细胞在Cyt c浓度为12.5mmol·L-1时细胞凋 亡的诱导率仍表现上升趋势;DNA电泳检测结果显示凋亡处理的细胞中DNA呈现较明显的DNA梯度。 展开更多
关键词 烟草 继代培养 悬浮细胞 诱导率 浓度 形态学特征 电泳检测 细胞色素c DNA 凋亡
下载PDF
关键蛋白酶激活因子Apaf-2/CytC在细胞凋亡中的作用 被引量:3
12
作者 陈荣林 李宏 刘建辉 《中国生物工程杂志》 CAS CSCD 北大核心 2006年第3期89-92,共4页
描述了Apaf2/CytC在细胞凋亡中的作用,主要阐述了细胞凋亡相关因子Apaf2/CytC的发现,Apaf2与Apaf1及Apaf3在细胞凋亡中的作用及相互关系,CytC介导细胞凋亡的方式。探讨了线粒体CytC的泄漏机制,对MPTP假说、专一性通道假说和线粒体膨胀... 描述了Apaf2/CytC在细胞凋亡中的作用,主要阐述了细胞凋亡相关因子Apaf2/CytC的发现,Apaf2与Apaf1及Apaf3在细胞凋亡中的作用及相互关系,CytC介导细胞凋亡的方式。探讨了线粒体CytC的泄漏机制,对MPTP假说、专一性通道假说和线粒体膨胀假说进行了阐述,并对细胞凋亡信号转导途径,如CytC途径、Fas/FasL途径、蛋白激酶途径、AIF凋亡途径及这些信号转导途径间的对话进行了分析,最后分析了研究CytC与细胞凋亡分子机制的意义并提出细胞凋亡分子机制中一些未完全研究清楚的问题。 展开更多
关键词 Apaf-2/cytc 细胞凋亡途径 线粒体 泄漏
下载PDF
颞叶内侧癫癎海马CA1区CytC、caspase-9、caspase-3蛋白的表达研究 被引量:2
13
作者 卢峰 杨朋范 +1 位作者 丁鑫 王如密 《中国微侵袭神经外科杂志》 CAS 北大核心 2009年第11期504-506,共3页
目的探讨凋亡相关蛋白细胞色素C(CytC)、caspase-9、caspase-3在颞叶内侧癫癎(MTLE)病人致癎海马CA1区神经元内的表达及意义。方法选择30例典型MTLE病人为实验组,6例颞叶深部血管畸形病人为对照组。采用苏木精-伊红染色、原位末端标记(T... 目的探讨凋亡相关蛋白细胞色素C(CytC)、caspase-9、caspase-3在颞叶内侧癫癎(MTLE)病人致癎海马CA1区神经元内的表达及意义。方法选择30例典型MTLE病人为实验组,6例颞叶深部血管畸形病人为对照组。采用苏木精-伊红染色、原位末端标记(TUNEL)方法在光镜下观察MTLE病人致癎海马CA1区神经元凋亡的情况。采用免疫组化S-P法检测并比较MTLE海马CA1区与对照组海马组织神经元内凋亡相关蛋白CytC、caspase-9、caspase-3的表达。结合病史资料,分析caspase-3表达水平与癫癎病程和发作频率的相关性。结果MTLE组致癎海马CA1区光镜下可观察到神经元退变、噬神经元现象,TUNEL染色发现凋亡神经元18例,对照组则为阴性;CytC、caspase-9、caspase-3蛋白在MTLE组海马CA1区均有明显表达,对照组中则呈轻微表达,差异显著(P<0.01)。caspase-3蛋白表达水平与发作频率呈正相关,而与病程长短无关。结论MTLE致癎海马神经元中存在外源性凋亡途径,这可能是癫癎发展的重要因素之一。 展开更多
关键词 癫痫 颞叶 海马 细胞凋亡 细胞色素c 半胱氨酸天冬氨酸蛋白酶
下载PDF
Cancer and age related colonic crypt deficiencies in cytochrome c oxidase Ⅰ 被引量:5
14
作者 Carol Bernstein Alexander Facista +9 位作者 Huy Nguyen Beryl Zaitlin Nadia Hassounah Cristy Loustaunau Claire Margaret Payne Bhaskar Banerjee Steve Goldschmid V Liana Tsikitis Robert Krouse Harris Bernstein 《World Journal of Gastrointestinal Oncology》 SCIE CAS 2010年第12期429-442,共14页
AIM: To investigate whether defi ciency of expressionof cytochrome c oxidase I (CcOI) in colonic crypts is associated with colon cancer.METHODS: The pattern and level of expression of CcOI in non-neoplastic colonic cr... AIM: To investigate whether defi ciency of expressionof cytochrome c oxidase I (CcOI) in colonic crypts is associated with colon cancer.METHODS: The pattern and level of expression of CcOI in non-neoplastic colonic crypts,and in dysplastic tissues,was assessed using standard immunohis-tochemical methods.Biopsies were obtained from individuals undergoing colonoscopies for screening purposes or for a medically indicated reason.Tissue samples were also obtained from surgical colonic resections.Samples from resections were taken from colonic mucosa 1 and 10 cm from tumors and from the tumors themselves.Samples were evaluated for frequency of crypts with reduced or absent expression of CcOI.In most crypts the loss was apparent throughout the entire crypt,while in a small minority the loss was segmental.The strong immunoreactivity using this monoclonal antibody makes the scoring unambiguous.The percent of crypts with reduced or absent expression of CcOI or (infrequent) segmented loss of expression was then calculated.Data analyses were performed using SPSS statistical package 17.0.RESULTS: The average frequency of CcOI deficient crypts (CcOI-DC) is low in individuals between 20 and 39 years of age,with 0.48% ± 0.40% CcOI-DC for women and 1.80% ± 0.35% for men.CcOI-DC increases after age 40 years,so that between the ages of 40 and 44 years the average frequency of CcOI- DC goes up to 5.89% ± 0.84% in women and 2.15% ± 1.27% in men.By 80-84 years of age,the average frequency of CcOI-DC goes up in women to 15.77% ± 0.97% and in men to 22.6% ± 0.65%.The increases in CcOI-DC from ages 40-44 years compared to 80-84 years in women and men are significantly different with P < 0.01.For women over age 60 years,deficiency of CcOI expression is greater in those women who have had a cancer in their colon.The frequency of CcOI-DC,measured in men,increased in tissues adjacent to colon cancer,being 4.03% ± 0.27% in individuals free of neoplasia in the age range 55-64 yearsand 14.13% ± 0.35% in resected histologically normal tissue of men with cancer in the same age range,P < 0.001.Similar signifi cant differences were noted in older age ranges.The frequency of CcOI-DC crypts in the cecum and sigmoid colon of an individual are signifi cantly correlated,with an R2 = 0.414 for women and R2 = 0.528 for men,P < 0.001.This suggests that the factors determining the level of CcOI deficiency act throughout the colon.Most defective crypts are in clusters of two or more,a likely consequence of crypt fission.In the non-neoplastic margins of cancers,crypts are frequently defi cient for CcOI,and such crypts may appear in large clusters,some containing more than 100 defi cient crypts.CcOI defi ciency is also apparent in colon cancers and sometimes involves a large section of the tumor.Overall,CcOI deficient cells can be visualized in segments of crypts,in whole crypts that increase in frequency with age,in crypts undergoing f ission,in clusters of crypts where the clusters increase in size with age,in increased frequency near tumors,in large clusters in the intimate margins of tumors,and in the tumors themselves.There is no clear dividing line between early stages that can be considered aspects of aging and later stages that can be considered aspects of the progression to cancer.This ambiguity may re ect a rather general situation leading to adult cancer where the early stages of cellular change appear to be relatively innocuous features of the aging process but over decades may evolve into malignancy.CONCLUSION: CcOI defi cient crypts increase in frequency with age,and clusters of defi cient crypts are associated with,and may give rise to,colon cancer. 展开更多
关键词 cytochrome c OXIDASE Aging colon cRYPTS colorectal cANcER Focal lesions
下载PDF
α-细辛醚上调细胞色素C及Caspase-3表达影响食管癌Eca-109细胞的生物学行为
15
作者 黄静 储韬 《南通大学学报(医学版)》 2024年第4期342-345,共4页
目的:分析α-细辛醚上调细胞色素C(cytochrome C,cytC)及Caspase-3表达对食管癌Eca-109细胞生物学行为的影响。方法:将食管癌Eca-109细胞随机等量分为4组,即低、中及高剂量(分别给予25、50及100 mg/L的α-细辛醚),另随机取等量细胞加入... 目的:分析α-细辛醚上调细胞色素C(cytochrome C,cytC)及Caspase-3表达对食管癌Eca-109细胞生物学行为的影响。方法:将食管癌Eca-109细胞随机等量分为4组,即低、中及高剂量(分别给予25、50及100 mg/L的α-细辛醚),另随机取等量细胞加入等体积培养液作为空白组,培养48 h后通过平板克隆实验检测4组细胞增殖情况,Annexin V/PI法检测细胞凋亡情况,RT-qPCR及Western Blot法检测细胞中cytC及Caspase-3 mRNA及蛋白表达。结果:(1)细胞克隆数在空白组最高,低剂量组显著高于高剂量组;凋亡率在空白组最低,低剂量组显著低于高剂量组。(2)4组细胞迁移至小室下的细胞数空白组>低剂量组>中剂量组>高剂量组。(3)CytC及Caspase-3蛋白在高剂量组表达显著高于空白组及低剂量组,且中剂量组表达高于空白组。(4)CytC及Caspase-3 mRNA的表达:高剂量组>中剂量组>低剂量组>空白组,差异均有统计学意义(均P<0.05)。结论:α-细辛醚抑制Eca-109细胞增殖及侵袭,并且可能通过上调cytC及Caspase-3表达促进调亡。 展开更多
关键词 Α-细辛醚 细胞色素c cASPASE-3 食管癌EcA-109细胞 增殖 凋亡
下载PDF
Artesunate Effect on Schistosome Thioredoxin Glutathione Reductase and Cytochrome c Peroxidase as New Molecular Targets in Schistosoma mansoni-infected Mice 被引量:2
16
作者 Amany A.Abdin Dalia S.Ashour Zeinab S.Shoheib 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2013年第12期953-961,共9页
Objective To investigate the possible effect of artesunate (ART) on schistosome thioredoxin glutathione reductase (TGR) and cytochrome c peroxidase (CcP) in Schistosoma mansoni-infected mice. Methods A total of ... Objective To investigate the possible effect of artesunate (ART) on schistosome thioredoxin glutathione reductase (TGR) and cytochrome c peroxidase (CcP) in Schistosoma mansoni-infected mice. Methods A total of 200 laboratory bred male Swiss albino mice were divided into 4 groups (50 mice in each group). Group I: infected untreated group (Control group) received a vehicle of 1% sodium carbonyl methylcellulose (CMC-Na); Group II: infected then treated with artesunate; Group III infected then treated with praziquantel, and group IV: infected then treated with artesunate then praziquantel. Adult S. mansoni worms were collected by Animal Perfusion Method, tissue egg counted, TGR, and CcP mRNA Expression were estimated of in $. mansoni adult worms by semi-quantitative rt-PCR. Results Semi-quantitative rt-PCR values revealed that treatment with artesunate caused significant decrease in expression of schistosome TGR and CcP in comparison to the untreated group. In contrast, the treatment with praziquantel did not cause significant change in expression of these genes. The results showed more reduction in total worm and female worm count in combined ART-PZQ treated group than in monotherapy treated groups by either ART or PZO, Moreover, complete disappearance (100%) of tissue eggs was recorded in ART-PZQ treated group with a respective reduction rate of 95.9% and 68.4% in ART- and PZQ-treated groups. Conclusion The current study elucidated for the first time that anti-schistosomal mechanisms of artesunate is mediated via reduction in expression of schistosome TGR and CcP. Linking these findings, addition of artesunate to praziquantel could achieve complete cure outcome in treatment of schistosomiasis. 展开更多
关键词 ScHISTOSOMIASIS ARTESUNATE PRAZIQUANTEL Thioredoxin glutathione reductase cytochrome c peroxidase
下载PDF
Effects of High Concentration Glucose on the Expression of NF-κB,Bax and Cytochrome C and Apoptosis of Islet Cells in Mice 被引量:2
17
作者 梁瑜祯 张木勋 +2 位作者 夏宁 杨月莲 冯乐平 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2009年第4期439-444,共6页
The roles of NF-kappaB (NF-κB) expression, Bax activity and cytochrome C (Cyt C) release, apoptosis of islet cells induced by high concentration glucose were explored in vitro. Pancreatic islet cells, which were ... The roles of NF-kappaB (NF-κB) expression, Bax activity and cytochrome C (Cyt C) release, apoptosis of islet cells induced by high concentration glucose were explored in vitro. Pancreatic islet cells, which were isolated from Kunming mice, were cultured with different concentrations of glucose in DMEM, and divided into the following groups: G1, G2, G3, G4, G5, and G6 groups, corresponding to the glucose concentrations of 5.6, 7.8, 11.1, 16.7, 22.5, and 27.6 mmol/L, respectively. After culture for 120 h, insulin secretion was evaluated by radioimmunoassay, and the NF-rd3 expression was detected by immunocytochemistry. Bax activity and Cyt C release were measured by immunofluorescence, and apoptosis was examined by Hoechst33342 assay. The results showed that in GI, G2 and G3 groups, insulin secretion was enhanced with the increase of glucose concentration, and the NF-κB expression was also increased (P〈0.05), but Bax activity, Cyt C release and apoptosis rate showed no significant difference among them. However, in G4, G5, and G6 groups, apoptosis rate of islet cells, NF-rd3 expression, Bax activity, and Cyt C release were all significantly increased, and insulin secretion was impaired as compared with G1, G2, and G3 groups (P〈0.05). It was concluded that the exposure of islet cells to high glucose could induce islet cells apoptosis as well as impaired insulin secretion. The NF-κB signaling pathway and mitochondria pathway in islet cells might play some roles in the progressive loss of islet cells in diabetes. The inhibition of the NF-κB expression could be an effective strategy for protecting pancreatic islet cells. 展开更多
关键词 islet cells APOPTOSIS high concentration glucose nuclear factor-κB cytochrome c BAX
下载PDF
Interaction of methylenetetrahydrofolate reductase C677T,cytochrome P4502E1 polymorphism and environment factors in esophageal cancer in Kazakh population 被引量:13
18
作者 Jiang-Mei Qin Lei Yang Bo Chen Xiu-Mei Wang Feng Li Pei-Hua Liao Lin He 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第45期6986-6992,共7页
AIM: To evaluate the association and interaction of genetic polymorphisms in methylenetetrahydrofolate reductase (MTHER) and cytochrome P4502E1 (CY- P4502E1), environment risk factors with esophageal cancer (EC... AIM: To evaluate the association and interaction of genetic polymorphisms in methylenetetrahydrofolate reductase (MTHER) and cytochrome P4502E1 (CY- P4502E1), environment risk factors with esophageal cancer (EC) in Kazakh, a high EC incidence area of Xinjiang Uygur Autonomous Region, China. METHODS: A 1:2 matched case-control study was conducted with 120 cases of EC and 240 populationor hospital-based controls. The controls were matched for sex, nationality, area of residence and age within a 5-year difference. MTHER and CYP4502E1 genotypes were identified by PCR-based restriction fragment length polymorphism (RFLP). A conditional logistic regression model was established to identify risk factors. The strata method was adopted in interaction analysis. RESULTS: Low consumption of green vegetables and fresh fruits, alcohol drinking, and unsafe water (shallow well, or river) were found to be the risk factors for EC. Individuals with the MTHFR677 (C/T + T/T) genotype had a 2.62-fold (95% CI: 1.61-4.28) risk of developing EC compared with those who carried the C/C genotype. Individuals with the CYP4502EIC1/C1 genotype had a 3.00-fold (95% CI: 1.82-4.96) risk compared with those who carried the CYP4502E1 (C1/C2 + C2/C2) genotype. Gene-environment interaction analysis showed that MTHFR677 gene polymorphism was correlated with consumption of green vegetables and fresh fruit, while CYP4502E1 C1/C1 was correlated with alcohol drinking and unsafe drinking water. MTHFR and CYP4502E1 analysis of gene-gene interaction showed that individuals with the MTHFR677 (C/T + T/T) and CYP4502EIC1/ C1 genotypes had a 7.41-fold (95% CI: 3.60-15.25) risk of developing EC compared with those who carried the MTHFR677C/C and CYP4502E1 RsaI C1/C2 + C2/C2 genes, and the interaction rate was higher than that of the two factors alone. CONCLUSION: Low consumption of green vegetables and fresh fruits, alcohol drinking, and unsafe water (shallow well, or river) and polymorphisms in MTHFR and CYP4502E1 genes are important risk factors for EC. There is a synergistic interaction among polymorphisms in MTHFR and CYP4502E1 genes and environment factors. MTHFR and CYP4502E1 genes can be used as biomarkers for prevention of EC in Kazakh, Xinjiang Uygur Autonomous Region, China. 展开更多
关键词 KAZAKH Esophageal cancer Methylenetet-rahydrofolate reductase c677T cytochrome P4502E1 Genetic polymorphism Environment risk factors INTERAcTION case control study
下载PDF
Mechanisms of Cytochrome C Extraction by Reverse Micelles 被引量:1
19
作者 YU Yan chun QIAN Bao hua +2 位作者 CHU Ying WU Zi sheng GAO Chang qing 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2001年第1期73-76,共4页
The extraction of cytochrome C was carried out by means of phase transfer technique with three different reverse micellar systems, i.e. , a CTAB micellar solution in n butyl alcohol chloroform(volume ratio 4... The extraction of cytochrome C was carried out by means of phase transfer technique with three different reverse micellar systems, i.e. , a CTAB micellar solution in n butyl alcohol chloroform(volume ratio 4∶1), an AOT micellar solution in isooctane and a SDSS D 2EHPA micellar solution in isooctane. The extraction mechanisms were studied. The results show that the extraction mechanisms for the same proteins with different types of reverse micellar systems can be distinct. The extraction of cytochrome C with CTAB and SDSS D 2EHPA reverse micellar systems are carried out according to the mechanism of electrostatic interaction. However, in the extraction of cytochrome C with the AOT reverse micellar system, the electrostatic interaction between the protein and the surfactant is not important. 展开更多
关键词 MEcHANISM cytochrome c EXTRAcTION Reverse micelles
下载PDF
Structure of Cytochromec and Its Platinum-modified Derivatives by Fourier Transform Infrared Spectroscopy 被引量:1
20
作者 JIANG Li-juan SUN Wei-yin +2 位作者 FANG Jiang-lin SHU Mou-hai TANG Wen-xia 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 1997年第2期3-11,共9页
The secondary structures of native cytochrome c(cyt c) in both solid and solution states and four platinum modified cyt c derivatives in solution were determined by means of Fourier transform infrared spectroscopy. I... The secondary structures of native cytochrome c(cyt c) in both solid and solution states and four platinum modified cyt c derivatives in solution were determined by means of Fourier transform infrared spectroscopy. It was found that the secondary structure of cyt c in solid state is similar to that in the solution. In the cases of platinum modified cyt c derivatives, when the binding sites of platinum complex are on or near the surface of the protein, its secondary structure is similar to that of native cyt c. However, when the platinum complex binds to Met 80 ligand and causes the replacement of the second axial ligand by non native Lys 79 ligand or H 2O supplied by solvent, there is a significant difference between the structures of low or high spin state cyt c derivatives and that of native cyt c. The results suggest that axial ligand Met 80 residue plays an important role in stabilizing the secondary structure of cyt c. 展开更多
关键词 cytochrome c PLATINUM Secondary structure FT IR
下载PDF
上一页 1 2 71 下一页 到第
使用帮助 返回顶部