A relatively sensitive, specific, and photostable method for the detection of cytokeratin of cancer cells via conjugation with cadmium telluride quantum dots（CdTe QDs） was described. Water soluble CdTe QDs were conj...A relatively sensitive, specific, and photostable method for the detection of cytokeratin of cancer cells via conjugation with cadmium telluride quantum dots（CdTe QDs） was described. Water soluble CdTe QDs were conjugated to anti-pan-cytokeratin（CK） monoclonal antibody（MAb） through coupling reagent [1-ethyl-3-（3-dimethyla- mino propyl）carbodiimide, EDC] and the conjugates were purified by dialysis. The expression of pan CK protein in HepG2 cells was observed by immunocytochemistry and direct immunofluorescence via QDs-Ab conjugates respectively. Fluorescence intensity and photostability of QDs were compared with those of FITC（fluorescein isothiocyanate）. The results show that the QDs-Ab conjugates recognized specifically pan CK protein in HepG2 cells. Compared with FITC, CdTe QDs had higher brightness and photostability without obvious photobleaching under continuous exciting light illumination for 30 min and after the placement at room temperature for 3 d. The results indicate that conjugates of CdTe quantum dot with anti-pan CK MAb can be used for labeling cancer cells derived from epithelial tissues, which provides the basis for the detection of circulating tumor cells（CTCs）.展开更多
Trichoepitheliomas are benign follicular appendage tumors with differentiation to all three segments of the hair follicle.A 2 years old female domestic short hair cat presented with a mass on the tail.The mass was sur...Trichoepitheliomas are benign follicular appendage tumors with differentiation to all three segments of the hair follicle.A 2 years old female domestic short hair cat presented with a mass on the tail.The mass was surgically excised and for histopathologic and immunohistochemical studies,was sent to Department of Pathology.Histologically,the tumor was encapsulated and consisted of many islands of follicular epithelium and also cysts structures which varied in size and shape.The cells of epithelium islands were round to oval and had variable amounts of slightly,eosinophilic cytoplasm and euchromatic nuclei.The cystic structures were lined by a complex layer of squamous epithelium.Often,cells under went an abrupt transition between basal layers and keratinization without the development of a granular cell layer.No tendency of malignancy was seen in this case.According to mentioned characteristics,trichoepitlielioma was diagnosed.By immunohistochemical study it was confirmed that this tumor had epithelial origin because squamous tumor cells reacted with the pan-cytokeratin antibody.The expression of p-catenin was predominately cytoplasmic and also together with numerous positive nuclei but membranous expression was inconsistenet.Distribution of neoplastic cells with P-catenin expression was more than 75%and labeling intensity was strong in both cytoplasm and nuclei.According to author's knowledge,this is the first report of trichoepithelioma in cat in Iran and also investigation of P-catenin expression in feline trichoepithelioma in veterinary literature.展开更多
基金Supported by the Major Project Fund of Jilin Provincial Science and Technology DepartmentChina(No.20082123)+1 种基金the Province University Union Fund of Jilin Province China(No.20082011)
文摘A relatively sensitive, specific, and photostable method for the detection of cytokeratin of cancer cells via conjugation with cadmium telluride quantum dots（CdTe QDs） was described. Water soluble CdTe QDs were conjugated to anti-pan-cytokeratin（CK） monoclonal antibody（MAb） through coupling reagent [1-ethyl-3-（3-dimethyla- mino propyl）carbodiimide, EDC] and the conjugates were purified by dialysis. The expression of pan CK protein in HepG2 cells was observed by immunocytochemistry and direct immunofluorescence via QDs-Ab conjugates respectively. Fluorescence intensity and photostability of QDs were compared with those of FITC（fluorescein isothiocyanate）. The results show that the QDs-Ab conjugates recognized specifically pan CK protein in HepG2 cells. Compared with FITC, CdTe QDs had higher brightness and photostability without obvious photobleaching under continuous exciting light illumination for 30 min and after the placement at room temperature for 3 d. The results indicate that conjugates of CdTe quantum dot with anti-pan CK MAb can be used for labeling cancer cells derived from epithelial tissues, which provides the basis for the detection of circulating tumor cells（CTCs）.
文摘Trichoepitheliomas are benign follicular appendage tumors with differentiation to all three segments of the hair follicle.A 2 years old female domestic short hair cat presented with a mass on the tail.The mass was surgically excised and for histopathologic and immunohistochemical studies,was sent to Department of Pathology.Histologically,the tumor was encapsulated and consisted of many islands of follicular epithelium and also cysts structures which varied in size and shape.The cells of epithelium islands were round to oval and had variable amounts of slightly,eosinophilic cytoplasm and euchromatic nuclei.The cystic structures were lined by a complex layer of squamous epithelium.Often,cells under went an abrupt transition between basal layers and keratinization without the development of a granular cell layer.No tendency of malignancy was seen in this case.According to mentioned characteristics,trichoepitlielioma was diagnosed.By immunohistochemical study it was confirmed that this tumor had epithelial origin because squamous tumor cells reacted with the pan-cytokeratin antibody.The expression of p-catenin was predominately cytoplasmic and also together with numerous positive nuclei but membranous expression was inconsistenet.Distribution of neoplastic cells with P-catenin expression was more than 75%and labeling intensity was strong in both cytoplasm and nuclei.According to author's knowledge,this is the first report of trichoepithelioma in cat in Iran and also investigation of P-catenin expression in feline trichoepithelioma in veterinary literature.
