P granules are germ granules contained in Caenorhabditis elegans germ cells.The first germ cell is specified by the one-cell embryo in which P granules localize to the posterior.Previous studies suggested that the mec...P granules are germ granules contained in Caenorhabditis elegans germ cells.The first germ cell is specified by the one-cell embryo in which P granules localize to the posterior.Previous studies suggested that the mechanism of the localization phenomena is induced by liquid-liquid phase transition(LLPT),in which the polarity proteins control the saturation point of P granules.In the present study,we propose that the P granules phase transition can be triggered by the cytoplasmic streaming.A two-phase flow model is employed to simulate the localization of P granules,i.e.,the cytoplasm is considered as a liquid phase,and the droplet-like P granules are another liquid phase.With the presence of the cytoplasmic streaming,P granules,initially distributing uniformly in the entire one-cell embryo,eventually condense/dissolve in the cytoplasm phase,regulated by difference between the saturation pressure and the hydrodynamic pressure.The numerical results reveal that the cytoplasmic streaming has significant effects on the localization of P granules,as well as the embryo division.展开更多
目的在大肠埃希菌中高效表达及纯化弓形虫GRA6抗原,为制作弓形虫感染基因工程诊断试剂盒奠定基础。方法将重组pGEX-GRA6表达载体转化大肠埃希菌BL21-Codon Plus (DE3)-RP菌株,在异丙基硫代-β-D半乳糖苷(IPTG)诱导下表达。超声破壁后,...目的在大肠埃希菌中高效表达及纯化弓形虫GRA6抗原,为制作弓形虫感染基因工程诊断试剂盒奠定基础。方法将重组pGEX-GRA6表达载体转化大肠埃希菌BL21-Codon Plus (DE3)-RP菌株,在异丙基硫代-β-D半乳糖苷(IPTG)诱导下表达。超声破壁后,通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析表达产物的表达形式,并对表达产物以硫酸铵沉淀、Sephedax G50脱盐和GST亲和层析柱进行目的蛋白的纯化。通过Western blotting检测其纯化的重组抗原的免疫反应性。结果GRA6以融合蛋白(GST-GRA6)的形式在大肠埃希菌中得到了高效表达。对表达产物可溶性分析表明,表达蛋白在上清液中和包涵体中均有表达。在上清液中表达的可溶性蛋白经纯化后蛋白纯度可达90%以上。Western blotting分析表明纯化蛋白能被弓形虫感染的人血清所识别。结论GRA6在大肠埃希菌中得到了高效表达,重组抗原经纯化后能特异性地被弓形虫感染者血清所识别。展开更多
2008249 Role of gonadotropin releasing hormone analogues for ovarian protection in systemic lupus erythematosus patients treated with cyclophosphamide.LIANG Liuqin(梁柳琴),et al.Dept Rheumatol Clin Immunol,1st Affili ...2008249 Role of gonadotropin releasing hormone analogues for ovarian protection in systemic lupus erythematosus patients treated with cyclophosphamide.LIANG Liuqin(梁柳琴),et al.Dept Rheumatol Clin Immunol,1st Affili Hosp Sun Yat-sen Univ,Guangzhou 510080.Natl Med J Chin 2008;88(15):1009-1011.Objective To evaluate the effectiveness of gonadotropin releasing hormone analogues(GnRH-a)in protection against premature ovarian failure during cyclophosphamide(CTX)展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.11272197,and 11372175)the Innovation Program of Shanghai Municipality Education Commission,China(Grant No.14ZZ095)
文摘P granules are germ granules contained in Caenorhabditis elegans germ cells.The first germ cell is specified by the one-cell embryo in which P granules localize to the posterior.Previous studies suggested that the mechanism of the localization phenomena is induced by liquid-liquid phase transition(LLPT),in which the polarity proteins control the saturation point of P granules.In the present study,we propose that the P granules phase transition can be triggered by the cytoplasmic streaming.A two-phase flow model is employed to simulate the localization of P granules,i.e.,the cytoplasm is considered as a liquid phase,and the droplet-like P granules are another liquid phase.With the presence of the cytoplasmic streaming,P granules,initially distributing uniformly in the entire one-cell embryo,eventually condense/dissolve in the cytoplasm phase,regulated by difference between the saturation pressure and the hydrodynamic pressure.The numerical results reveal that the cytoplasmic streaming has significant effects on the localization of P granules,as well as the embryo division.
文摘2008249 Role of gonadotropin releasing hormone analogues for ovarian protection in systemic lupus erythematosus patients treated with cyclophosphamide.LIANG Liuqin(梁柳琴),et al.Dept Rheumatol Clin Immunol,1st Affili Hosp Sun Yat-sen Univ,Guangzhou 510080.Natl Med J Chin 2008;88(15):1009-1011.Objective To evaluate the effectiveness of gonadotropin releasing hormone analogues(GnRH-a)in protection against premature ovarian failure during cyclophosphamide(CTX)
