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RAPD and ISSR Markers of Fertility Restoring Gene for Aegilops kotschyi Cytoplasmic Male Sterility in Wheat 被引量:10
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作者 刘保申 孙其信 +6 位作者 孙兰珍 高庆荣 解超杰 窦秉德 倪中福 魏艳玲 张延传 《Acta Botanica Sinica》 CSCD 2002年第4期446-450,共5页
LK783 was found to be a good fertility restorer for K-type male sterility of wheat (Triticum aestivum L.). RAPD and ISSR (inter-simple sequence repeat polymorphism) markers were employed to map the major restoring gen... LK783 was found to be a good fertility restorer for K-type male sterility of wheat (Triticum aestivum L.). RAPD and ISSR (inter-simple sequence repeat polymorphism) markers were employed to map the major restoring gene in LK783. Maintainer and restorer DNA pools were established using the extreme sterile and fertile plants among KJ5418A//911289/LK783 F 1 population, respectively. Four hundred and eighteen RAPD primers and 33 ISSR primers were used for screening polymorphisms between the two pools, and amplification bands using a RAPD primer of OPK18 and an ISSR primer of UBC-845 were found polymorphic between the two pools. Linkage analysis showed that OPK18 450 and UBC-845 800 were linked to the restoring gene in LK783. The distance between the restoring gene and OPK18 450 was (15.07±6.28) cM (centiMorgan), with the distance between the restoring gene and UBC-845 800 being (8.20±4.85) cM. The marker of UBC-845 800 was located on chromosome 1BS by amplifying nulli-tetrasomics and 1B ditelosomics of Chinese Spring with the primer of UBC-845, indicating that the restoring gene in LK783 was located on 1BS. The breeding for new fertility restorer lines of K-type cytoplasmic male sterility of wheat would be facilitated by using the two markers. 展开更多
关键词 WHEAT cytoplasmic male sterility restoring gene inter-simple sequence repeat polymorphism RAPD
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Microtubule Structure and Male Sterility in a Gene-Cytoplasmic Male Sterile Line of Rice, Zhen Shan 97A 被引量:4
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作者 叶秀麟 杨子德 +1 位作者 徐是雄 梁承邺 《Acta Botanica Sinica》 CSCD 2003年第2期183-192,共10页
Histological changes that occur during microsporogenesis are documented in a gene-cytoplasmic male sterile rice ( Oryza saliva L.) line, Zhen Shan 97A, its maintainer line, Zhen Shan 97B, and the restorer line, Ce64 o... Histological changes that occur during microsporogenesis are documented in a gene-cytoplasmic male sterile rice ( Oryza saliva L.) line, Zhen Shan 97A, its maintainer line, Zhen Shan 97B, and the restorer line, Ce64 of a Mine hybrid rice production system. In the restorer line, Ce64, the developing microsporocytes have dense cytoplasm and a distinct set of circumferential microtubules around the nucleus. Successive cytokinesis results in the formation of tetrads. The microtubules within the cells of tetrads and microspores radiate from the surface of the nucleus towards the outer edge of the cytoplasm. Subsequent pollen development is normal. During the course of microspore formation tubulin speckles can be found in the cytoplasm. The general pattern of development and microtubule organization in the maintainer lined Zhen Shan 97B, is similar to Ce64, except that a few more tubulin speckles appear during microspore formation. In the case of the mate sterile line, Zhen Shan 97A, a number of abnormalities can be discerned during early microsporogenesis. These include vacuoles forming within the developing microsporocyte and faintly stained microtubules with no defined distribution pattern. Prominent tubulin speckles are common within the cytoplasm. For those microsporocytes that undergo meiosis, no defined organizational patterns of microtubules can be found within the tetrad. All microspores abort soon after. Abnormalities and defects in microtubule organization observed in Zhen Shan 97A showed that complex interactions between the cytoplasm and the nucleus began at very early stage of microsporocyte development. 展开更多
关键词 Oryza saliva cytoplasmic male sterility MICROTUBULES microsporocyte VACUOLE tubulin speckles
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Mapping of Fertility Restoring Gene for Aegilops kotschyi Cytoplasmic Male Sterility in Wheat Using SSR Markers 被引量:13
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作者 LIU Bao-shen, SUN Qi-xin, GAO Qing-rong, SUN Lan-zhen, XIE Chao-jie, LI Chuan-you, NI Zhong-fu and DOU Bing-de( Agronomy Department, Shandong Agricultural University, Taian 271018, P.R.China Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100094 , P. R . China ) 《Agricultural Sciences in China》 CAS CSCD 2002年第8期845-849,共5页
LK783 was found to be a good fertility restorer for K-type male sterility of wheat. Microsatel-lite markers were employed to map the major restoring gene in LK783. Maintainer and restorer DNA pools were established us... LK783 was found to be a good fertility restorer for K-type male sterility of wheat. Microsatel-lite markers were employed to map the major restoring gene in LK783. Maintainer and restorer DNA pools were established using the extreme sterile and fertile plants among (KJ5418A//911289/LK783)F1 population, respectively. Seventy-nine sets of SSR primers were screened for polymorphism between the two pools, 6 of which were found polymorphic. Linkage analysis showed that Xgwm11, Xgwm18, Xgwm264a and Xgwm273 were linked to the restoring gene in LK783, while Xgwm11, Xgwm18 and Xgwm273 were co-segregated. The distance between the Rf gene in LK783 and the three co-segregated markers was 6.54 ± 4.37 cM, the distance between Rf gene and Xgwm264a was 5. 71 ± 4.10 cM. The four SSR markers were located on chromosome IBS by amplifying the DNA of nulli-tetrasomics and ditelosomics of CS with the 4 sets of primers, indicating that the major restoring gene in LK783 was located on IBS, but the relative location of the gene was different from Rfv1, allelism of the two genes should be further investigated. The breeding for new fertility restorer lines of K-type cytoplasmic male sterility in wheat would be facilitated by using the four polymorphic markers. 展开更多
关键词 Wheat ( Triticum aestivum) cytoplasmic male sterility Restoring gene Molecular marker MICROSATELLITE
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Abortive Process of a Novel Rapeseed Cytoplasmic Male Sterility Line Derived from Somatic Hybrids Between Brassica napus and Sinapis alba 被引量:5
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作者 WANG Juan GAO Ya-nan +4 位作者 KONG Yue-qin JIANG Jin-jin LI Ai-min ZHANG Yong-tai WANG You-ping 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2014年第4期741-748,共8页
Somatic hybridization is performed to obtain significant cytoplasmic male sterility (CMS) lines, whose CMS genes are derived either from the transfer of sterile genes from the mitochondrial genome of donor parent to... Somatic hybridization is performed to obtain significant cytoplasmic male sterility (CMS) lines, whose CMS genes are derived either from the transfer of sterile genes from the mitochondrial genome of donor parent to the counterpart of receptor or production of new sterile genes caused by mitochondrial genome recombination of the biparent during protoplast fusion. In this study, a novel male sterile line, SaNa-IA, was obtained from the somatic hybridization between Brassica napus and Sinapis alba. The normal anther development of the maintainer line, SaNa-IB, and the abortive process of SaNa-IA were described through phenotypic observations and microtome sections. The floral organ of the sterile line SaNa-IA was sterile with a shortened filament and deflated anther. No detectable pollen grains were found on the surface of the sterile anthers. Semi-thin sections indicated that SaNa-IA aborted in the pollen mother cell (PMC) stage when vacuolization of the tapetum and PMCs began. The tapetum radically elongated and became highly vacuolated, occupying the entire locule together with the vacuolated microspores. Therefore, SaNa-IA is different from other CMS lines, such as ogu CMS, pol CMS and nap CMS as shown by the abortive process of the anther. 展开更多
关键词 Brassica napus anther abortion cytoplasmic male sterility (CMS) semi-thin sections somatic hybridization
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Mitochondrial Proteomic Analysis of Cytoplasmic Male Sterility Line and Its Maintainer in Wheat (Triticum aestivum L.) 被引量:2
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作者 CHEN Rui-hong LIU Wei ZHANG Gai-sheng YE Jing-xiu 《Agricultural Sciences in China》 CAS CSCD 2010年第6期771-782,共12页
To investigate the CMS mechanism of wheat on proteomic level and find the crucial proteins which related to fertility,mitochondria was isolated from young spike of wheat by differential centrifugation and Percoll dens... To investigate the CMS mechanism of wheat on proteomic level and find the crucial proteins which related to fertility,mitochondria was isolated from young spike of wheat by differential centrifugation and Percoll density-gradient methods.Determined by marker enzyme assays and chlorophyll content,the mainly contaminants in the spike mitochondrial fraction were caused by peroxisomes,plastids and chloroplasts after the first discontinuous Percoll density gradient centrifugation.In order to improve the purity of spike mitochondria,a second 28% Percoll self-forming density gradient centrifugation was further carried out,the result showed that the contaminants were decreased to negligible amount,meanwhile the integrity of mitochondria (88%) was improved to 90%.The spike mitochondria proteins extracted from uninucleate stage of (S)-1376A and (A)-1376B were separated by two-dimensional electrophoresis (2-DE),and the silver stained gels were analyzed by PDQuest 2-DE software,about 326 protein spots could be visualized on the 2-DE maps,and also revealed a similar pattern between the male sterile line and its maintainer line,except 11 spots were differentially expressed.A total of five differentially expressed proteins were analyzed by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS),three of them were identified as manganese superoxide dismutase and T5E216 following NCBInr database by the Mascot software.These results may contribute to further understanding of the mechanisms of CMS in wheat. 展开更多
关键词 MITOCHONDRIA proteomics cytoplasmic male sterility two-dimensional electrophoresis MALDI-TOF-MS
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Development of Japonica Male Sterile Lines Integrating Cytoplasmic Male Sterility and Photosensitive Genic Male Sterility 被引量:2
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作者 WANG Shou-hai DU Shi-yun WANG De-zheng LI Cheng-quan 《Agricultural Sciences in China》 CAS CSCD 2005年第12期883-889,共7页
Acknowledgement It has been previously established that the BT type of cytoplasmic male sterility (CMS) is induced by high temperatures, while photosensitive genic male sterility (PGMS) seed sets by low temperatur... Acknowledgement It has been previously established that the BT type of cytoplasmic male sterility (CMS) is induced by high temperatures, while photosensitive genic male sterility (PGMS) seed sets by low temperatures induce. In the current study, we have bred photosensitive cytoplasmic male sterility (PCMS) lines (2308SA and 2310SA) by crossing the CMS line with the PGMS japonica line with maintainer genes. The sterility of PCMS japonica was consequently controlled by two groups of male sterile genes resulting from the integration of PGMS and CMS genes. The results on plant fertility, at different sowing times, were as follows: (a) Under conditions of natural long-day photoperiod and at temperatures above 35~C, the PGMS gene regulated PCMS japonica sterility - the higher the temperature, the lower the pollen fertility. However, bagged seed sets of PCMS japonica, not exposed to high temperatures, induced the CMS seed set. (b) Exposure to long-day photoperiod and temperature conditions between 35℃ and the critical sterility inducing temperature of PGMS resulted in both PGMS and CMS gene controlled sterility of PCMS japonica, which exhibited stable characteristics. (c) When exposed to critical sterility inducing temperatures or short-day photoperiod and daily high temperatures below 32℃, the BT type of the CMS gene regulated PCMS sterility. Under these conditions, the PGMS gene rendered male sterility insusceptible to occasional cool summer days when this PCMS line, adopted for hybrid seed production, develops into panicle differentiation stage. The present study also investigated the fertility restoration, seed production and combining ability of PCMS japonica so as to optimize its use. 展开更多
关键词 Oryza sativa var. japonica Photosensitive genic male sterility cytoplasmic male sterility GENE Photosensitive cytoplasmic male sterility
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Establishment and Identification of Cytoplasmic Male Sterility in Brassica napus by Intergeneric Somatic Hybridization 被引量:2
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作者 HUQiong LIYun-chang +3 位作者 MEIDe-sheng FANGXiao-ping LiseNHansen SvenBAndersen 《Agricultural Sciences in China》 CAS CSCD 2003年第12期1321-1328,共8页
Exploitation of novel cytoplasmic male sterility (CMS) is a main approach for widening the cytoplasmic genetic background of hybrid oilseed rape and avoiding epidemic risk in oilseed rape production. In this study, sy... Exploitation of novel cytoplasmic male sterility (CMS) is a main approach for widening the cytoplasmic genetic background of hybrid oilseed rape and avoiding epidemic risk in oilseed rape production. In this study, symmetric somatic hybrids between Brassica napus var. Zhongshuang4 and Sinapis arvensis (Ye- you18) were produced by protoplast fusion. Two of the six established hybrids were male sterile showing trace or no pollen release upon flowering with non- or slightly extended stamens. Using Zhongshuang4 as a recurrent parent to pollinate the male sterile plants, the ratio of male sterile plants increased with the number of backcrosses. As early as in BC 3 generation, most of the sterile families had nearly 100% sterile plants. Up to BC 4 generation, the male sterility became stable and no fertility segregation was observed. All F 1 progenies from tested crosses using restorer and maintainer lines of Polima CMS were 100% sterile, indicating that the established CMS by somatic hybridization is different from Polima CMS. The origin of the cytoplasm and potential use of this novel CMS in oilseed rape breeding were discussed. 展开更多
关键词 Oilseed rape Protoplast fusion cytoplasmic male sterility Sinapis arvensis
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Nectar secretion of RN-type cytoplasmic male sterility three lines in soybean [Glycine max(L.) Merr.] 被引量:5
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作者 ZHANG Jing-yong SUN Huan +4 位作者 ZHAO Li-mei ZHANG Chun-bao YAN Hao PENG Bao LI Wen-bin 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2018年第5期1085-1092,共8页
Significant progress has been achieved in the use of heterosis in soybean and several soybean hybrids have been released in China. However, broad use of hybrid soybean seed is limited due to low seed setting of female... Significant progress has been achieved in the use of heterosis in soybean and several soybean hybrids have been released in China. However, broad use of hybrid soybean seed is limited due to low seed setting of female parents. Breeding cytoplasmic male sterile(CMS) lines with high out-crossing rate is necessary to solve the problem. The objective of this study was to determine the relationship between out-crossing rate of CMS lines and their nectar secretion. The daily nectar secretion rhythm, meteorological effect on nectar secretion, and differences in nectar secretion among genotypes and years were investigated in 27 soybean CMS lines(A-lines) with their maintainers(B-lines) and restorers(R-lines). The correlation between out-crossing rate of CMS lines and nectar production was also evaluated. Nectar secretion had diurnal variation. Secretion initiated at about 06:00 for most materials and reached a peak at 07:00–08:30 after flower opened, then the nectar secretion decreased gradually. A sub-peak appeared at about 13:00, while the nectar could not be detected at 17:00. Nectar secretion was greatly influenced by the weather conditions. The amount of nectar secretion increased gradually over time during periods of high temperature and no rainfall for several days. Rainy weather and low temperatures inhibited nectar secretion. There were obvious variations of nectar amount among different genotypes tested. Significant nectar variation within a genotype among years was also observed, and the highest nectar secretion was 3-fold higher than the lowest. The amount of nectar secretion from R-lines was significantly higher than that of A-and B-lines. There was no significant difference in nectar secretion between A-and B-lines. A-and B-lines with higher out-crossing rates secreted more nectar. The amount of nectar secretion of A-and B-lines were significantly positively correlated with the out-crossing rate of A-lines. 展开更多
关键词 soybean RN cytoplasmic male sterility nectar secretion out-crossing rate
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Mapping of Rice Fertility-Restoring Genes for ID-Type Cytoplasmic Male Sterility in a Restorer Line R68 被引量:1
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作者 LI Liang-jie ZHOU Hai-peng ZHAN Xiao-deng CHENG Shi-hua CAO Li-yong 《Rice science》 SCIE 2008年第2期157-160,共4页
An F2 population derived from the cross Zhong 9A/R68 was used to map the fertility-restoring (Rf) gene for ID-type cytoplasmic male sterility (CMS). Two bulks (a fertile bulk and a sterile bulk) were constructed... An F2 population derived from the cross Zhong 9A/R68 was used to map the fertility-restoring (Rf) gene for ID-type cytoplasmic male sterility (CMS). Two bulks (a fertile bulk and a sterile bulk) were constructed by pooling equal amount of ten highly fertile lines and ten highly sterile lines, respectively. Four hundred and thirteen pairs of simple sequence repeat (SSR) primers, which evenly distributed on 12 chromosomes of rice, were selected for analyzing polymorphisms between the parents and between the two bulks. The primer RM283 on chromosome 1 and the primers RM5756, RM258, RM6100 and RM171 on chromosome 10 were found to be polymorphic between the parents and between the two bulks. These five SSR markers were linked to fertility-restoring genes. A total of 82 excessive sterile lines were selected from Zhong 9A/R68 F2 population to estimate the genetic distance between five SSR markers and fertility-restoring genes respectively. The results indicated that one Rf gene was linked to RM283 located on chromosome 1 at a distance of 6.7 cM, and the other Rfgene was mapped to the long arm of chromosome 10 flanked by RM258 and RM6100 at the distances of 8.0 cM and 2.4 cM, respectively. 展开更多
关键词 RICE cytoplasmic male sterility fertility-restoring gene gene mapping
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Cytoplasmic Male Sterility in Maize 被引量:1
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作者 RONG Ting-zhao, LI Wan-chen, CAO Mo-ju and HU Chang-yuan( Maize Research Institute, Sichuan Agricultural University, Ya ’ an 625014 ,P.R. China ) 《Agricultural Sciences in China》 CAS CSCD 2002年第10期1100-1104,共5页
14 isoplasmic and allonuclear cytoplasmic male sterile lines were used as female parents, 8 tester lines as male parents, 101 F, progenies were obtained. Fertility restoration response of 101 F] progenies were investi... 14 isoplasmic and allonuclear cytoplasmic male sterile lines were used as female parents, 8 tester lines as male parents, 101 F, progenies were obtained. Fertility restoration response of 101 F] progenies were investigated through field observation and pollen stainability examination under microscope. 14 isoplasmic and allonuclear cytoplasmic male sterile lines were developed by repeated backcross with recurrent male parent lines for more than 8 generations. The result shows: tester line Zifengl not only restored the isoplasmic and allonuclear sterile lines of group C backcrossed with Mo17, Yu30 and Heer, but also completely restored the isoplasmic and allonuclear cytoplasm male sterile lines of group T backcrossed with Mo17, HZS , 1792 , 292 and Yu30. Therefore, nuclear background limits the use of Zifengl as a tester for identification of cytoplasmic male sterility. Furthermore RFLPs of mitochondrial DNA of 6 isonuclear and alloplasmic cytoplasmic male sterile lines were analyzed with BamH I and Hind 1 restriction endonuclease and mitochondrial DNA probes pBcmH3 and Cox II . The same RFLPs were found within sterile cytoplasm of group C, including C, Chuan G, Lei 2 and Lei 3, but a different RFLP pattern was observed among sterile cytoplasm of group S, C, T and the normal cytoplasm. This result suggested that the RFLP markers tightly linked to sterile mitochon-drial genes of different groups could be applied in the identifcation of cytoplasmic male sterility. 展开更多
关键词 Zea mays L cytoplasmic male sterility RFLP FERTILITY
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Inheritance of Fertility Restoration for Cytoplasmic Male Sterility in a New Gossypium barbadense Restorer 被引量:1
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作者 ZHANG Xiao-quan WANG Xue-de JIANG Pei-dong ZHU Wei 《Agricultural Sciences in China》 CAS CSCD 2010年第4期472-476,共5页
In order to clarify inheritance mechanism of fertility restoration for cytoplasmic male sterility (CMS) in a new Gossypium barbadense restorer line Hai R which was found in the fertility test crossing of G. hirsutum... In order to clarify inheritance mechanism of fertility restoration for cytoplasmic male sterility (CMS) in a new Gossypium barbadense restorer line Hai R which was found in the fertility test crossing of G. hirsutum CMS lines with G. barbadense germplasms. 23 fertility segregation populations of F2 and backcross were used to analyze the inheritance of fertility restoring gene(s) of Hai R. The result showed that Hai R had one major dominant gene (RfB) to control the CMS fertility restoration and this fertility restoration gene functioned at the sporophytic level. The sterile cytoplasm background might not only influence the transmission rate of male gamete but also that of female gamete when the restorer gene was recessive. It could be deduced that this fertility restoration gene might come from G. harknessii cotton, Hai R is of value in the application of cotton interspecific hybrid breeding. 展开更多
关键词 Gossypium barbadense cytoplasmic male sterility fertility restoring gene
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Development of Transgenic Restorer of Cytoplasmic Male Sterility in Upland Cotton 被引量:1
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作者 WANG Xue-de and LI Yue-you( College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029 ,P.R. China) 《Agricultural Sciences in China》 CAS CSCD 2002年第4期375-380,共6页
A glutathione S-transferase gene (gst) has been introduced into restorers of cytoplasmic male sterility in upland cotton (Gossypium hirsutum L.) using Agrobacterium-mediated transformation. A trans-genie restorer, sig... A glutathione S-transferase gene (gst) has been introduced into restorers of cytoplasmic male sterility in upland cotton (Gossypium hirsutum L.) using Agrobacterium-mediated transformation. A trans-genie restorer, signed as 'Zheda strong restorer', which has strong restorability to male sterility, was selected from progeny plants of transformants. When compared with an American restorer 'DES-HAF277', the fertility restorability of 'Zheda strong restorer' to male sterility was been enhanced by 25.8% in the percentage of viable pollens of hybrid (sterile line × restorer) F1 The hybrid gave 3.6 more bolls per plant, 10.1% less aborted seeds and 10.6% more lint yield when 'Zheda strong restorer' was used as male parent than when 'DES-HAF277' was. Southern and Northern bloting analysis showed that the foreign gsf gene was detectable and highly expressed in 'Zheda strong restorer'. 展开更多
关键词 Upland cotton cytoplasmic male sterility Transgenic restorer
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Influence of Soil Moisture and Air Temperature on the Stability of Cytoplasmic Male Sterility (CMS) in Maize (Zea mays L.) 被引量:2
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作者 Heidrun Bueckmann Katja Thiele Joachim Schiemann 《Agricultural Sciences》 2016年第2期70-81,共12页
Cytoplasmic male sterility (CMS) is a maternally inherited trait that suppresses the production of viable pollen. CMS is a useful biological tool for confinement strategies to facilitate coexistence of genetically mod... Cytoplasmic male sterility (CMS) is a maternally inherited trait that suppresses the production of viable pollen. CMS is a useful biological tool for confinement strategies to facilitate coexistence of genetically modified (GM) and non-GM crops in case where it is required. The trait is reversible and can be restored to fertility in the presence of nuclear restorer genes (Rf genes) and by environmental impacts. The aim of this study was to investigate the influence of the level of irrigation on the stability of CMS maize hybrids under defined greenhouse conditions. Additionally the combination of irrigation and air temperature was studied. Three CMS maize hybrids were grown with different levels of irrigation and in different temperature regimes. Tassel characteristics, pollen production and fertility were assessed. The CMS stability was high in hot air temperatures and decreased in lower temperatures. The level of irrigation had no major effect on the level of sterility. The extent of these phenomena was depending on the genotype of CMS maize and should be known before using CMS for coexistence purposes. 展开更多
关键词 Soil Moisture Air Temperature Biological Confinement cytoplasmic male sterility (CMS) Genetically Modified (GM) Maize (Zea mays L.)
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Development of New InDel Marker to Detect Genotypes of Rf-1a Conferring Fertility Restoration of BT-Type Cytoplasmic Male Sterility in Rice
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作者 CHEN Tao ZHANG Ya-dong +6 位作者 ZHU Zhen ZHAO Ling ZHAO Qing-yong ZHOU Li-hui YAO Shu YU Xin WANG Cai-lin 《Rice science》 SCIE 2014年第1期13-19,共7页
Restorer line breeding is an important approach to enhance the heterosis and improve the yields of japonica hybrid rice. To improve the selection efficiency of restorer lines for BT-type cytoplasmic male sterility (... Restorer line breeding is an important approach to enhance the heterosis and improve the yields of japonica hybrid rice. To improve the selection efficiency of restorer lines for BT-type cytoplasmic male sterility (CMS) in japonica rice, a functional marker InDeI-Rf-la based on the difference of nucleotide sequence in Rf-la locus between BT-type CMS lines and restorer lines was developed to detect the genotypes of different rice materials. Conventional indica rice varieties, restorer and maintainer lines without 574 bp deletion could restore the fertility for BT-type CMS in japonica rice. By contrast, most conventional japonica rice varieties except Aichi 106 and Yijing 12, with genotype of rf-larf-la showed the 574 bp deletion maintained sterility for BT-type CMS lines. To further verify the effect of genotyping detection in Rf-la locus, this marker was also used to amplify the genomic DNA in different japonica rice restorer lines, CMS lines, hybrids and F2 segregation population, and three genotypes in Rf-la locus could be distinguished distinctly. Therefore, the marker InDeI-Rf-la could be widely used for genetic id^ntifio.~tinn ~nd m^rkp.r-~.~.~i^fp.d .~.tAr.tinn (MA.~ in hr~=dinn i^nnnir~ r^fnr~=r lin==~ 展开更多
关键词 BT-type cytoplasmic male sterility fertility restoration Rf-la gene InDel marker japonica rice
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Isolation and Characterization of the Cytoplasmic Male Sterility Associated Gene of Cotton(Gossypium harknessii)
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作者 WU Jian-yong,GONG Yang-chang,XING Chao-zhu,GUO Li-ping(Cotton Research Institute of CAAS,Key Laboratory of Cotton Genetic Improvement,Ministry of Agriculture,Anyang,Henan 455000,China) 《棉花学报》 CSCD 北大核心 2008年第S1期71-,共1页
Cytoplasmic male sterility(CMS) is a maternally inherited trait that results in the failure to produce functional pollen.It was identified in many plants,and it is widely used to exploit heterosis.
关键词 Gossypium harknessii Isolation and Characterization of the cytoplasmic male sterility Associated Gene of Cotton GENE
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Genetic analysis of fertility restoration of Maxie cytoplasmic male sterility in rice(Oryza sativa L.)
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作者 ZHANG Xiaoguo ZHU Yingguo and MEI Qiming,College of Life Sci,Wuhan Univ,Wuhan 430072,China 《Chinese Rice Research Newsletter》 1996年第4期1-2,共2页
The genetic behavior of fertility restorationgenes of the cytoplasmic male sterile line Max-ie A was studied to facilitate the use ofMaweizhan male sterile cytoplasm.The F,F,and Fof Maxie A/Minhui 63 were grownin padd... The genetic behavior of fertility restorationgenes of the cytoplasmic male sterile line Max-ie A was studied to facilitate the use ofMaweizhan male sterile cytoplasm.The F,F,and Fof Maxie A/Minhui 63 were grownin paddy field,1993-1995.Meanwhile,someof the Fplants were testcrossed with themaintainer of Maxie A.The fertilities were 展开更多
关键词 Oryza sativa L Genetic analysis of fertility restoration of Maxie cytoplasmic male sterility in rice
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Influential Factors of Restorer of New Cytoplasmic Male Sterile(NER) on Anther Culture 被引量:11
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作者 李浩杰 蒲晓斌 +2 位作者 张锦芳 张启行 蒋梁材 《Agricultural Science & Technology》 CAS 2008年第1期49-53,共5页
Some influential factors of anther culture were studied preliminarily by conducting anther culture of the restorers of new cytoplasmic male sterile (NER). Several results were obtain from this experiment and they we... Some influential factors of anther culture were studied preliminarily by conducting anther culture of the restorers of new cytoplasmic male sterile (NER). Several results were obtain from this experiment and they were listed as follow:① MS cultrure medium with such hormones as 2,4-D 2 mg/L,6-BA 0.5 mg/L, NAA 0.5 mg/L was the best suitable for callus induction of NER. ②The difference of induction rate was significantly different between different plant age groups. From the 110th day to 141th day,the induction rate was increased with the increase of age and the difference of induction rate reached 0.01 significant difference level. The induction rate reached the highest value in the 141th day then it declined gradually. ③The combined use of 2, 4-D and 6-BA with proper increase of 2,4-D was good for inducing callus. ④The green plantlet induction rate of NER was increased when the concentration of 6-BA increased from 2 mg/L to 4 mg/L. Adding ZT from 0.5 mg/L to 2 mg/L. 6-BA would led 2.47% increase of green plantlet olantlet induction rate. 展开更多
关键词 Brassica napus L New cytoplasmic male sterile(NEA) Restorer of new cytoplasmic male sterile(NER) Anther culture
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Preliminary Studies on the Effect of Photoperiod and Temperature on Male Fertility of a Cytoplasmic Male Sterile Line in Wheat 被引量:2
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作者 王丰青 巍荷 +1 位作者 何觉民 何仪 《Agricultural Science & Technology》 CAS 2008年第2期85-89,共5页
[Objective] The study aimed to reveal the effect of photoperiod and temperature on male fertility of cytoplasmic male sterile line Vtai911289a in wheat and discuss the mechanism of male fertility alteration. [ Method ... [Objective] The study aimed to reveal the effect of photoperiod and temperature on male fertility of cytoplasmic male sterile line Vtai911289a in wheat and discuss the mechanism of male fertility alteration. [ Method ] The sowing-date tests and designed conditions were conducted during 2003 -2005. [ Result] Fertility of Vtai911289a, could alter under specific photoperiod and temperature conditions. Temperature is one of the main factors influencing male fertility of the male sterile lines. Vtai911289a, showed stable sterility under the condition of the mean of daily temperature at fertility sensitive stage lower than 19℃ and presented partial fertility when the mean of daily temperature at fertility sensitive stage lower than 20 - 22℃. Photoperiod to some extent affects the male fertility of Vtai911289a, long-day condition is helpful for the male fertility of the sterile line. [ Conclusion] The application of photoperiod temperature-sensitive cytoplasmic male sterile line in production has a higher safety than that of temperature sensitive sterile line. 展开更多
关键词 Temperature PHOTOPERIOD cytoplasmic male sterile line male fertility
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Cytological Study on Zidao Type Cytoplasmic Male Sterile Line-Yingxiang A 被引量:2
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作者 魏磊 《Agricultural Science & Technology》 CAS 2015年第3期459-461,共3页
Anthers of male sterile line and its maintainer line of Zidao type rice, Yingxiang A and Yingxiang B were studied by cytological methods, such as F-KI staining, silver nitrate staining and paraffin sections, It is fou... Anthers of male sterile line and its maintainer line of Zidao type rice, Yingxiang A and Yingxiang B were studied by cytological methods, such as F-KI staining, silver nitrate staining and paraffin sections, It is found that in Yingxiang A, there are some abnormalities in meiosis, represented by unequal division at the meiotic phase; the abortion of CMS line arises at the meiotic phase and is almost completed at the phase of monomicrospore, but only a tiny amount of aborted mi- crospores are observed; the abortion is complete and thorough, and none normal functional pollens are found; the morphology of aborted pollens is similar to that of typically aborted pollens; the tapetal cells still exist in anthers from the microsporocyte stage to the tetrad stage, but they are abnormally hyperplastic. However, in Yingxiang B, there are no abnormalities in meiosis at the meiotic phase; the tapetal cells exist in anthers before the tetrad stage, but they disappear completely when the pollen are mature. It is indicated that the abortion of CMS Yingxiang line mainly occurs at the meiotic phase, and is basically completed at the phase of monomicrospore. The abortion is probably caused by the abnormally hyperplastic tapetal cells. The abnormalities in tapetal cells may affect the normal development of pollens, resulting in the emergence of abortion. In addition, the abortion in Yingxiang A is complete and thorough, which is of great significance in hybrid breeding. 展开更多
关键词 cytoplasmic male sterility Cytological Study TAPETUM
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Establishment on Isolated Microspore Culture Optimized System for Restorer of New Cytoplasmic Male Sterile (NER) of Brassica napus L.
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作者 李浩杰 蒲晓斌 +5 位作者 张锦芳 张德发 夏凊 石化娟 蒋俊 蒋梁材 《Agricultural Science & Technology》 CAS 2010年第1期182-185,共4页
[Objective]The aim was to establish optimized system for NER isolated microspore culture of Brassica napus L.. [Method]Twenty varieties of NER of Brassica napus were grown under uncontrolled temperature and light cond... [Objective]The aim was to establish optimized system for NER isolated microspore culture of Brassica napus L.. [Method]Twenty varieties of NER of Brassica napus were grown under uncontrolled temperature and light conditions,and their isolated microspore from anthers were used as explants in vitro culture. The influencing factors of microspore culture were preliminarily studied. [Result]The difference of genotypes was important influencing factors to embryoid yield. The embryoid yield increased by supplementing with 6-BA and NAA,culturing in solid-liquid double layer medium with activated charcoal; The difference was not significant of embryoid yield between culturing in medium supplemented with colchicines and the CK. The rates of cotyledonous embryoids directly developed into normal plantlets increased through enriching with 0.1-0.2 mg/L NAA and being treated on slim illumination two days before being inducted into normal plantlets. [Conclusion]The technical system of microspore culture of restorer of new cytoplasmic male sterile (NER) was established,which and lays a foundation for accelerating genotype purification of NER introgressive line. 展开更多
关键词 Brassica napus L. Restorer of new cytoplasmic male sterile (NER) Isolated microspore culture Technical system
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