AIM To identify hepatitis C virus (HCV) core protein epitopes recognized by HLA-A2 restricted cytotoxic T lymphocyte (CTL).METHODS Utilizing the method of computer prediction followed by a 4 h 51 Cr-release assay conf...AIM To identify hepatitis C virus (HCV) core protein epitopes recognized by HLA-A2 restricted cytotoxic T lymphocyte (CTL).METHODS Utilizing the method of computer prediction followed by a 4 h 51 Cr-release assay confirmation.RESULTS The results showed that peripheral blood mononuclear cells (PBMC) obtained from two HLA-A2 positive donors who were infected with HCV could lyse autologous target cells labeled with peptide 'ALAHGVFAL (core TS0-158)'.The rates of specific lysis of the cells from the two donors were 37.5% and 15.8%,respectively. Blocking of the CTL response with anti-CD4 mAb caused no significant decrease of the specific lysis.But blocking of CTL response with anti-CD8 mAb could abolish the Iysis.CONCLUSION The peptide (core 150 - 158 ) is the candidate epitope recognized by HLA-A2 restricted CTL.展开更多
Objective:With the development of peptide-based cancer specific immunotherapy,the prediction of CTL epitopes from insulin-like growth factor-binding protein 7(IGFBP7) is very important for some research about tumor me...Objective:With the development of peptide-based cancer specific immunotherapy,the prediction of CTL epitopes from insulin-like growth factor-binding protein 7(IGFBP7) is very important for some research about tumor metastasis.Because HLA-A2.1-expressing individuals cover >50% in the population of China,we aimed at identifying IGFBP7-encoded peptide presented by HLA-A2.1.Methods:In our study,a HLA-A2.1 restricted CTL epitope was identified by using the following two-step procedure:(a) computer-based epitope prediction from the amino acid sequence of IGFBP7 antigen;(b) Validation with epitope molecular modeling.Results:We obtained four epitopes with high immunogenicity scores by all of the three algorithms,i.e.,BIMAS,SYFPEITHI and IMTECH.Each of the four candidates satisfied the criteria of the HLA-A2.1-restricted CTL epitopes in molecular modeling analysis.Conclusion:The combination of BIMAS,SYFPEITHI and IMTECH method can improve the prediction efficiency and accuracy.Due to this research herein,this four epitopes have potential value for further studied,also have potential application in peptide-mediated immunotherapy.These epitopes may be useful in the design of therapeutic peptide vaccine for lung carcinoma and as immunotherapeutic strategies against lung carcinoma after identified by immunology experiment.展开更多
基金the National Nature Science Foundation of China,No.39800121
文摘AIM To identify hepatitis C virus (HCV) core protein epitopes recognized by HLA-A2 restricted cytotoxic T lymphocyte (CTL).METHODS Utilizing the method of computer prediction followed by a 4 h 51 Cr-release assay confirmation.RESULTS The results showed that peripheral blood mononuclear cells (PBMC) obtained from two HLA-A2 positive donors who were infected with HCV could lyse autologous target cells labeled with peptide 'ALAHGVFAL (core TS0-158)'.The rates of specific lysis of the cells from the two donors were 37.5% and 15.8%,respectively. Blocking of the CTL response with anti-CD4 mAb caused no significant decrease of the specific lysis.But blocking of CTL response with anti-CD8 mAb could abolish the Iysis.CONCLUSION The peptide (core 150 - 158 ) is the candidate epitope recognized by HLA-A2 restricted CTL.
基金Supported by the Special fund of the National High Technology Research and Development Program of China (863 Program,2007AA02Z129)the National Natural Science Foundation of China (30672076 and 30800506)
文摘Objective:With the development of peptide-based cancer specific immunotherapy,the prediction of CTL epitopes from insulin-like growth factor-binding protein 7(IGFBP7) is very important for some research about tumor metastasis.Because HLA-A2.1-expressing individuals cover >50% in the population of China,we aimed at identifying IGFBP7-encoded peptide presented by HLA-A2.1.Methods:In our study,a HLA-A2.1 restricted CTL epitope was identified by using the following two-step procedure:(a) computer-based epitope prediction from the amino acid sequence of IGFBP7 antigen;(b) Validation with epitope molecular modeling.Results:We obtained four epitopes with high immunogenicity scores by all of the three algorithms,i.e.,BIMAS,SYFPEITHI and IMTECH.Each of the four candidates satisfied the criteria of the HLA-A2.1-restricted CTL epitopes in molecular modeling analysis.Conclusion:The combination of BIMAS,SYFPEITHI and IMTECH method can improve the prediction efficiency and accuracy.Due to this research herein,this four epitopes have potential value for further studied,also have potential application in peptide-mediated immunotherapy.These epitopes may be useful in the design of therapeutic peptide vaccine for lung carcinoma and as immunotherapeutic strategies against lung carcinoma after identified by immunology experiment.
