[Objective] This study aimed to screen an Na＋/H＋ antiporter gene from the halophiles colonizing in the Dagong Ancient Brine Well in Zigong City, China, and then analyze the gene structure and properties of the prote...[Objective] This study aimed to screen an Na＋/H＋ antiporter gene from the halophiles colonizing in the Dagong Ancient Brine Well in Zigong City, China, and then analyze the gene structure and properties of the protein encoded by this gene. [Method] Metagenomic DNA libraries of halophiles from the Dagong Ancient Brine Well were used for screening genes with Na＋/H＋ antiporter activity in antiporter-defi- cient E. coil KNabc strain by functional complementation. Then the start codon, stop codon, ORF, -35 region, -10 region and SD sequence of Na~/H＋ antiporter gene, as well as the molecular weight, isoelectric point, hydrophobic region, transmembrane domain, phyletic evolution and salt resistance of protein encoded by the gene were investigated. [Result] A new Na＋/H＋ antiporter gene m-nha was obtained, which ,ren- dered the antiporter-negative mutant E. coil KNabc cells with both the resistance to Na＋ and the ability to grow under alkaline conditions. [Conclusion] The structure and amino acid sequence of M-Nha was different from the previously reported Na＋/H~ antiporters, and the m-nha gene disclosed from the Dagong Ancient Brine Well was identified as a novel Na＋/H＋ antiporter gene. This study was significant not only in helping us understand the salt tolerance of halophiles in ancient brine wells and develop and utilize the genes resource, but also in exploring new salt-tolerant genes.展开更多
Neither comrades nor yet full-fledged consumers, Chinese dagongmei （female migrant workers） and their experience（s） are an essential part of the story of China＇s globalizing economy. The growing body of scholarly...Neither comrades nor yet full-fledged consumers, Chinese dagongmei （female migrant workers） and their experience（s） are an essential part of the story of China＇s globalizing economy. The growing body of scholarly literature on internal migrant workers exposes a chilling underside to China＇s passionate embrace of market-oriented reform--which certainly should give pause to those otherwise inclined to hail the rise of global capitalism as a glorious shift toward democracy and greater personal freedom. My particular interest in the subject lies in the intersection of the rural-to-urban labor migration phenomenon, gender, and the realm of contemporary cultural expression. This article examines the diverse array of literary and visual representations of dagongmei, a site of distinctly post-Mao ideological contestation. It begins with a discussion of dagongmeiwithin popular and mainstream media. It then turns to the broad historical and cultural contexts that have spurred the grassroots emergence of worker literature in recent decades in southern China. The article concludes with an analysis of work by two women writers--poet Zheng Xiaoqiong and novelist Wang Lili--that offers highly gendered accounts of the contemporary migrant labor experience.展开更多
基金Supported by Chunhui Plan of Ministry of Education(Z2010101)Open Fund of Food Biotechnology Key Laboratory of Sichuan Province(SZJJ2009-014)Scientific Research Foundation of Xihua University(000022)~~
文摘[Objective] This study aimed to screen an Na＋/H＋ antiporter gene from the halophiles colonizing in the Dagong Ancient Brine Well in Zigong City, China, and then analyze the gene structure and properties of the protein encoded by this gene. [Method] Metagenomic DNA libraries of halophiles from the Dagong Ancient Brine Well were used for screening genes with Na＋/H＋ antiporter activity in antiporter-defi- cient E. coil KNabc strain by functional complementation. Then the start codon, stop codon, ORF, -35 region, -10 region and SD sequence of Na~/H＋ antiporter gene, as well as the molecular weight, isoelectric point, hydrophobic region, transmembrane domain, phyletic evolution and salt resistance of protein encoded by the gene were investigated. [Result] A new Na＋/H＋ antiporter gene m-nha was obtained, which ,ren- dered the antiporter-negative mutant E. coil KNabc cells with both the resistance to Na＋ and the ability to grow under alkaline conditions. [Conclusion] The structure and amino acid sequence of M-Nha was different from the previously reported Na＋/H~ antiporters, and the m-nha gene disclosed from the Dagong Ancient Brine Well was identified as a novel Na＋/H＋ antiporter gene. This study was significant not only in helping us understand the salt tolerance of halophiles in ancient brine wells and develop and utilize the genes resource, but also in exploring new salt-tolerant genes.
文摘Neither comrades nor yet full-fledged consumers, Chinese dagongmei （female migrant workers） and their experience（s） are an essential part of the story of China＇s globalizing economy. The growing body of scholarly literature on internal migrant workers exposes a chilling underside to China＇s passionate embrace of market-oriented reform--which certainly should give pause to those otherwise inclined to hail the rise of global capitalism as a glorious shift toward democracy and greater personal freedom. My particular interest in the subject lies in the intersection of the rural-to-urban labor migration phenomenon, gender, and the realm of contemporary cultural expression. This article examines the diverse array of literary and visual representations of dagongmei, a site of distinctly post-Mao ideological contestation. It begins with a discussion of dagongmeiwithin popular and mainstream media. It then turns to the broad historical and cultural contexts that have spurred the grassroots emergence of worker literature in recent decades in southern China. The article concludes with an analysis of work by two women writers--poet Zheng Xiaoqiong and novelist Wang Lili--that offers highly gendered accounts of the contemporary migrant labor experience.
