目的:探讨清化颗粒对db/db糖尿病小鼠肠道苦味受体(taste receptor type 2,TAS2Rs)的影响。方法:将db/db糖尿病小鼠随机分为模型对照组及清化颗粒低、中、高剂量组,db/m小鼠为空白对照组。清化颗粒低、中、高剂量组分别灌胃给药(3.77、7...目的:探讨清化颗粒对db/db糖尿病小鼠肠道苦味受体(taste receptor type 2,TAS2Rs)的影响。方法:将db/db糖尿病小鼠随机分为模型对照组及清化颗粒低、中、高剂量组,db/m小鼠为空白对照组。清化颗粒低、中、高剂量组分别灌胃给药(3.77、7.54、15.08 g·kg-1·d-1),模型对照组和空白对照组灌胃等体积的生理盐水,均干预4周。干预结束后,腹腔注射葡萄糖耐量试验(IPGTT)观察每组小鼠血糖水平变化;ELISA法分析小鼠血清胰高血糖素样肽-1(GLP-1)浓度;qRT-PCR测定每组小鼠肠道苦味受体(TAS2R7、TAS2R9和TAS2R38)的mRNA水平;Western blot检测小鼠肠道苦味信号通路因子磷酸二酯酶1A(PDE1A)的蛋白水平。结果:清化颗粒各剂量组的空腹血糖水平均较模型对照组显著降低(P<0.01),血清GLP-1浓度显著高于模型对照组(P<0.01);清化颗粒中、高剂量组的TAS2R7、TAS2R9和TAS2R38的mRNA水平较模型对照组显著增加(P<0.01);清化颗粒各剂量组的PDE1A的蛋白水平较模型对照组显著增加(P<0.01),呈剂量依赖效应。结论:清化颗粒能够降低db/db糖尿病小鼠的空腹血糖水平,其机制可能与促进肠道GLP-1分泌的苦味信号通路有关。展开更多
The leaves of Aquilaria sinensis（Lour.） Gilg have been used in folk medicine for the treatment of diabetes in Guangdong Province,China.In this study,effects of ethanol extract of Aquilaria sinensis leaves on hypergl...The leaves of Aquilaria sinensis（Lour.） Gilg have been used in folk medicine for the treatment of diabetes in Guangdong Province,China.In this study,effects of ethanol extract of Aquilaria sinensis leaves on hyperglycemia were investigated in diabetic db/db mice.After 4 weeks of administration,the 95%ethanol（EtOH） extract of Aquilaria sinensis leaves （AE）,especially at high dose level（600 mg/kg）,activated AMP-activated protein kinase（AMPK）,resulting in reduced fasting blood glucose and glycosylated hemoglobin levels in db/db mice.In addition,the oral glucose tolerance test（OGTT test） showed that AE could remarkably improve insulin resistance.Compared to Thiazolinediones（TZDs）,no weight gain was observed after AE administration,which is a severe side effect of TZDs.The data suggested that AE could act as an activator of AMPK,and might be used as an alternative to TZDs in the management of obesity-related diabetes.展开更多
基金New-Century Talent Program,Ministry of Educating of China(Grant No.985-2-102-113)National Key Technology R&D Program"New Drug Innovation"of China(Grant No.2009ZX09311-004,2012ZX09301-002-002-002)
文摘The leaves of Aquilaria sinensis（Lour.） Gilg have been used in folk medicine for the treatment of diabetes in Guangdong Province,China.In this study,effects of ethanol extract of Aquilaria sinensis leaves on hyperglycemia were investigated in diabetic db/db mice.After 4 weeks of administration,the 95%ethanol（EtOH） extract of Aquilaria sinensis leaves （AE）,especially at high dose level（600 mg/kg）,activated AMP-activated protein kinase（AMPK）,resulting in reduced fasting blood glucose and glycosylated hemoglobin levels in db/db mice.In addition,the oral glucose tolerance test（OGTT test） showed that AE could remarkably improve insulin resistance.Compared to Thiazolinediones（TZDs）,no weight gain was observed after AE administration,which is a severe side effect of TZDs.The data suggested that AE could act as an activator of AMPK,and might be used as an alternative to TZDs in the management of obesity-related diabetes.
