DNA甲基化作为表观遗传学的一个重要调节机制,对基因调控和基因组稳定性至关重要。DDM1(DECREASED DNA METHYLATION 1)是维持正常基因组DNA甲基化模式所需的ATP依赖性SWI2/SNF2染色质重塑因子。DDM1对于维持全基因组DNA甲基化水平,特别...DNA甲基化作为表观遗传学的一个重要调节机制,对基因调控和基因组稳定性至关重要。DDM1(DECREASED DNA METHYLATION 1)是维持正常基因组DNA甲基化模式所需的ATP依赖性SWI2/SNF2染色质重塑因子。DDM1对于维持全基因组DNA甲基化水平,特别是异染色质区的转座子的DNA甲基化水平是不可缺少的。DDM1具有稳定转座子活性、转变核小体的结构等功能。此外,研究表明DDM1在DNA损伤反应中起作用。本综述主要对DNA甲基化、DDM1的发现、结构特征和DDM1及其同源基因在植物中的研究进展进行概括,并对其在不同物种中的表现差异进行分析和展望,为DNA甲基化的研究提供参考。展开更多
Clubroot caused by the protist Plasmodiophora brassicae is a major disease affecting cultivated Brassica-ceae.Using a combination of quantitative trait locus(QTL)fine mapping,CRISPR-Cas9 validation,and extensive analy...Clubroot caused by the protist Plasmodiophora brassicae is a major disease affecting cultivated Brassica-ceae.Using a combination of quantitative trait locus(QTL)fine mapping,CRISPR-Cas9 validation,and extensive analyses of DNA sequence and methylation patterns,we revealed that the two adjacent neigh-boring NLR(nucleotide-binding and leucine-rich repeat)genes AT5G47260 and AT5G47280 cooperate in controlling broad-spectrum quantitative partial resistance to the root pathogen P.brassicae in Arabidopsis and that they are epigenetically regulated.The variation in DNA methylation is not associated with any nucleotide variation or any transposable element presence/absence variants and is stably inherited.Vari-ations in DNA methylation at the Pb-At5.2 QTL are widespread across Arabidopsis accessions and corre-late negatively with variations in expression of the two genes.Our study demonstrates that natural,stable,and transgenerationally inherited epigenetic variations can play an important role in shaping resistance to plant pathogens by modulating the expression of immune receptors.展开更多
文摘DNA甲基化作为表观遗传学的一个重要调节机制,对基因调控和基因组稳定性至关重要。DDM1(DECREASED DNA METHYLATION 1)是维持正常基因组DNA甲基化模式所需的ATP依赖性SWI2/SNF2染色质重塑因子。DDM1对于维持全基因组DNA甲基化水平,特别是异染色质区的转座子的DNA甲基化水平是不可缺少的。DDM1具有稳定转座子活性、转变核小体的结构等功能。此外,研究表明DDM1在DNA损伤反应中起作用。本综述主要对DNA甲基化、DDM1的发现、结构特征和DDM1及其同源基因在植物中的研究进展进行概括,并对其在不同物种中的表现差异进行分析和展望,为DNA甲基化的研究提供参考。
文摘Clubroot caused by the protist Plasmodiophora brassicae is a major disease affecting cultivated Brassica-ceae.Using a combination of quantitative trait locus(QTL)fine mapping,CRISPR-Cas9 validation,and extensive analyses of DNA sequence and methylation patterns,we revealed that the two adjacent neigh-boring NLR(nucleotide-binding and leucine-rich repeat)genes AT5G47260 and AT5G47280 cooperate in controlling broad-spectrum quantitative partial resistance to the root pathogen P.brassicae in Arabidopsis and that they are epigenetically regulated.The variation in DNA methylation is not associated with any nucleotide variation or any transposable element presence/absence variants and is stably inherited.Vari-ations in DNA methylation at the Pb-At5.2 QTL are widespread across Arabidopsis accessions and corre-late negatively with variations in expression of the two genes.Our study demonstrates that natural,stable,and transgenerationally inherited epigenetic variations can play an important role in shaping resistance to plant pathogens by modulating the expression of immune receptors.
