This study was carried out to determine the effect of replacement of forage fiber sources from alfalfa and Leymus chinensis with nonforage fiber sources(NFFS) from dried distillers grains with solubles and corn germ...This study was carried out to determine the effect of replacement of forage fiber sources from alfalfa and Leymus chinensis with nonforage fiber sources(NFFS) from dried distillers grains with solubles and corn germ meal on calves growth, rumen development and blood parameters. 48 female and 12 male calves((110.55±15.36) kg of body weight and 12 wk of age) were assigned randomly to four dietary treatments(15 calves/treatment) in a completely randomized design. Experimental diets were: 0% NFFS(control), 9% NFFS(group 1), 18% NFFS(group 2), 27% NFFS(group 3), and contained equivalent neutral detergent fiber and total digestible nutrients, respectively. The dry matter intake was similar among diets, averaging 3.33 kg d-1, and no differences were detected for body weight, withers height, body length and heart girth. In addition, the development of rumen, reticulum, omasum and abomasum also were similar among diets. Dry matter, crude protein, and neutral detergent fiber digestibilities increased with the increasing levels of NFFS in the experimental diets, but had no significant effect. Blood urea nitrogen, total protein and glubulin were not affected by the dietary treatment, but group 3 resulted in the highest(P〈0.05) concentrations of glucose and the lowest(P〈0.05) concentrations of triglycerides and albumin. In conclusion, dried distillers grains with solubles(DDGS) and corn germ meal(CGM) were available and alternative fiber sources for Holstein calf diets.展开更多
It is very difficult to directly spin the lignocellulose without pretreatment.Ionic liquids(ILs)are promising solvent to dissolve lignocellulose to prepare cellulose fiber.However,the degree of cellulose polymerizatio...It is very difficult to directly spin the lignocellulose without pretreatment.Ionic liquids(ILs)are promising solvent to dissolve lignocellulose to prepare cellulose fiber.However,the degree of cellulose polymerization(DP)is reduced when lignocellulose is dissolved in ILs,and the lignin removal rate is low.The elongation at break and tensile strength of the fibers obtained by spinning the lignocellulose dissolved in ILs are poor.In this paper,preparing cellulose fiber directly from lignocellulose based on dissolving corn stalk via[C4mim]Cl-L-arginine binary system is achieved.It shows that the removal rate of lignin can reach 92.35%and the purity of cellulose can reach 85.32%after corn stalk was dissolved at 150℃C for 11.5 h when the mass fraction of arginine is 2.5%.The elongation at break of fiber reached 10.12%and the tensile strength reached 420 MPa.It is mainly due to the fact that L-arginine not only inhibits the degradation of cellulose but also promotes the delignination.Without any pulping or pretreatment,preparing cellulose fibers via direct dissolution and extrusion may provide a simple and effective way to prepare many novel cellulose materials.展开更多
[Objective] The paper was to study the effects of different levels of NaOH on fiber, crude protein and soluble sugar content of cornstraw. [Method] Four treatments were designed in the test, namely control group, grou...[Objective] The paper was to study the effects of different levels of NaOH on fiber, crude protein and soluble sugar content of cornstraw. [Method] Four treatments were designed in the test, namely control group, group 1, group2 and group 3, which were added with 0, 2%, 3%and 4% NaOH according to the dry weight of corn straw, and the moisture of corn straw after treatment was 45%. After being treated at room tem-perature for 48 h, sensory evaluation and nutrient composition analysis were performed for each group, and the contents of neutral detergent fiber(NDF), acid detergent fiber (ADF), crude protein (CP) and soluble sugar (SS) were measured. [Result] With the increasing addition level of NaOH,the content of NDF and ADF decreased gradually, and significant differences were observed between group 3 (adding 4% NaOH) and control group(P〈0.05). There was no significant difference in CP content among treatment groups(P〉0.05); the SS content gradually increased, and there weresignificant differences between treatment groups and control group (P〈0.05). [Conclusion] 4% NaOH reduced the fiber content and increased thesoluble sugar content of corn straw.展开更多
A feruloyl esterase (FAE-C6) gene of 957 bp was isolated from rumen microbial metagenome, subcloned into pET32b vector, and expressed in </span><i><span style="font-family:Verdana;">Escheri...A feruloyl esterase (FAE-C6) gene of 957 bp was isolated from rumen microbial metagenome, subcloned into pET32b vector, and expressed in </span><i><span style="font-family:Verdana;">Escherichia</span></i><i><span style="font-family:Verdana;"> coli</span></i><span style="font-family:Verdana;">. The enzyme purified in active form, consisted of 319 amino acid residues, with a molecular weight of 43.7 based on SDS-PAGE. Homology modeling showed that the FAE contained the catalytic triad composed of Ser</span><sub><span style="font-family:Verdana;">154-</span></sub><span style="font-family:Verdana;">Asp</span><sub><span style="font-family:Verdana;">263</span></sub><span style="font-family:Verdana;">His</span><sub><span style="font-family:Verdana;">295</span></sub><span style="font-family:Verdana;"> and a classical Gly-X-Ser</span><sub><span style="font-family:Verdana;">154</span></sub><span style="font-family:Verdana;">-X-Gly nucleophile motif commonly found in esterases. The FAE-C6 was characterized using corn fiber as substrate. Its combining action with glycoside hydrolases (C, X, A) individually and in various combinations was studied with focus on the difference in</span></span><span style="font-family:Verdana;"> the</span><span style="font-family:Verdana;"> effects on FA and sugar release. Glycoside hydrolases with endo-xylanase included in the enzyme mixture showed significant impact on increasing the FA yield. For the release of sugar, FAE enhanced the yield in all hydrolase combinations moderately and endo-xylanase was not the key factor in the enzyme formulation.展开更多
The corn husks were usually discarded as useless materials,after alkali treatment from which the corn husk fiber was obtained. The corn husk fiber included half degumming corn husk bundle fiber and corn husk single fi...The corn husks were usually discarded as useless materials,after alkali treatment from which the corn husk fiber was obtained. The corn husk fiber included half degumming corn husk bundle fiber and corn husk single fiber. This study found that corn husk single fiber was a kind of cellulose fiber,and was obtained with the fully alkali treatment technique,the process of which was NaOH concentration 0.15 g/mL,temperature at 80℃,and reaction time about 2.5 h. The morphologies of corn husk single fibers presented nature convolutions along with the fiber axis. They were closed at both ends,and they had a pentagram cavity and oval-shaped crosssection. They were flat shape,the fineness of the fibers was close to cotton fiber,and the mechanical properties of the fibers were similar to hemp fiber. So the corn husk fibers could be predicted that they could be used in textile industry because their properties were very close to cotton fiber or flax fiber.展开更多
In our modern world, where conserving energy is highly valued, thermal insulation panels play a crucial role in reducing heat transfer between two spaces, surfaces, or materials. They are used to enhance the energy ef...In our modern world, where conserving energy is highly valued, thermal insulation panels play a crucial role in reducing heat transfer between two spaces, surfaces, or materials. They are used to enhance the energy efficiency of various industrial applications by minimizing heat loss and temperature control. These panels function as silent protectors, aiding in reducing energy consumption and making things more sustainable and better for the environment. This is where composite materials come in;they are known for their lightweight nature, high strength-to-weight ratio, and excellent thermal insulation properties and have gained significant attention. Researchers are actively engaged in various studies aimed at enhancing these materials further. This research project focuses on the development of kaolin and glass fiber-reinforced composites for thermally insulating panels, to which natural strengthening materials like corn husk and bamboo fibers are added. The aim is to create cost-effective and efficient composite materials for thermal insulation applications by incorporating these components with a binder consisting of potassium silicate, hydroxide, and distilled water. This project involves conducting compression tests, bending tests, impact tests, thermal conductivity measurements, and microscopic analysis to evaluate the mechanical and thermal properties of the developed composites. The profound impact of these engineered composites on thermal insulation panels stands to revolutionize energy conservation efforts, offering a potent avenue to minimize heat loss and enhance overall energy efficiency across an array of industrial sectors.展开更多
基金supported by the National Key Technologies during the 12th Five-Year Plan period (2011BAD17B03)
文摘This study was carried out to determine the effect of replacement of forage fiber sources from alfalfa and Leymus chinensis with nonforage fiber sources(NFFS) from dried distillers grains with solubles and corn germ meal on calves growth, rumen development and blood parameters. 48 female and 12 male calves((110.55±15.36) kg of body weight and 12 wk of age) were assigned randomly to four dietary treatments(15 calves/treatment) in a completely randomized design. Experimental diets were: 0% NFFS(control), 9% NFFS(group 1), 18% NFFS(group 2), 27% NFFS(group 3), and contained equivalent neutral detergent fiber and total digestible nutrients, respectively. The dry matter intake was similar among diets, averaging 3.33 kg d-1, and no differences were detected for body weight, withers height, body length and heart girth. In addition, the development of rumen, reticulum, omasum and abomasum also were similar among diets. Dry matter, crude protein, and neutral detergent fiber digestibilities increased with the increasing levels of NFFS in the experimental diets, but had no significant effect. Blood urea nitrogen, total protein and glubulin were not affected by the dietary treatment, but group 3 resulted in the highest(P〈0.05) concentrations of glucose and the lowest(P〈0.05) concentrations of triglycerides and albumin. In conclusion, dried distillers grains with solubles(DDGS) and corn germ meal(CGM) were available and alternative fiber sources for Holstein calf diets.
基金This research was financially supported by the National Natural Science Foundation of China(No.21878292,No.21606240,No.21878314)K.C.Wong Education Foundation(No.GJTD-2018-04)the Strategic Priority Research Program of Chinese Academy of Science(No.XDA21060300).
文摘It is very difficult to directly spin the lignocellulose without pretreatment.Ionic liquids(ILs)are promising solvent to dissolve lignocellulose to prepare cellulose fiber.However,the degree of cellulose polymerization(DP)is reduced when lignocellulose is dissolved in ILs,and the lignin removal rate is low.The elongation at break and tensile strength of the fibers obtained by spinning the lignocellulose dissolved in ILs are poor.In this paper,preparing cellulose fiber directly from lignocellulose based on dissolving corn stalk via[C4mim]Cl-L-arginine binary system is achieved.It shows that the removal rate of lignin can reach 92.35%and the purity of cellulose can reach 85.32%after corn stalk was dissolved at 150℃C for 11.5 h when the mass fraction of arginine is 2.5%.The elongation at break of fiber reached 10.12%and the tensile strength reached 420 MPa.It is mainly due to the fact that L-arginine not only inhibits the degradation of cellulose but also promotes the delignination.Without any pulping or pretreatment,preparing cellulose fibers via direct dissolution and extrusion may provide a simple and effective way to prepare many novel cellulose materials.
基金Supported by PhD Start-up Fund of Inner Mongolia University for Nationalities(BS291)
文摘[Objective] The paper was to study the effects of different levels of NaOH on fiber, crude protein and soluble sugar content of cornstraw. [Method] Four treatments were designed in the test, namely control group, group 1, group2 and group 3, which were added with 0, 2%, 3%and 4% NaOH according to the dry weight of corn straw, and the moisture of corn straw after treatment was 45%. After being treated at room tem-perature for 48 h, sensory evaluation and nutrient composition analysis were performed for each group, and the contents of neutral detergent fiber(NDF), acid detergent fiber (ADF), crude protein (CP) and soluble sugar (SS) were measured. [Result] With the increasing addition level of NaOH,the content of NDF and ADF decreased gradually, and significant differences were observed between group 3 (adding 4% NaOH) and control group(P〈0.05). There was no significant difference in CP content among treatment groups(P〉0.05); the SS content gradually increased, and there weresignificant differences between treatment groups and control group (P〈0.05). [Conclusion] 4% NaOH reduced the fiber content and increased thesoluble sugar content of corn straw.
文摘A feruloyl esterase (FAE-C6) gene of 957 bp was isolated from rumen microbial metagenome, subcloned into pET32b vector, and expressed in </span><i><span style="font-family:Verdana;">Escherichia</span></i><i><span style="font-family:Verdana;"> coli</span></i><span style="font-family:Verdana;">. The enzyme purified in active form, consisted of 319 amino acid residues, with a molecular weight of 43.7 based on SDS-PAGE. Homology modeling showed that the FAE contained the catalytic triad composed of Ser</span><sub><span style="font-family:Verdana;">154-</span></sub><span style="font-family:Verdana;">Asp</span><sub><span style="font-family:Verdana;">263</span></sub><span style="font-family:Verdana;">His</span><sub><span style="font-family:Verdana;">295</span></sub><span style="font-family:Verdana;"> and a classical Gly-X-Ser</span><sub><span style="font-family:Verdana;">154</span></sub><span style="font-family:Verdana;">-X-Gly nucleophile motif commonly found in esterases. The FAE-C6 was characterized using corn fiber as substrate. Its combining action with glycoside hydrolases (C, X, A) individually and in various combinations was studied with focus on the difference in</span></span><span style="font-family:Verdana;"> the</span><span style="font-family:Verdana;"> effects on FA and sugar release. Glycoside hydrolases with endo-xylanase included in the enzyme mixture showed significant impact on increasing the FA yield. For the release of sugar, FAE enhanced the yield in all hydrolase combinations moderately and endo-xylanase was not the key factor in the enzyme formulation.
基金Science and Technology Project of Fujian Province,China(No.2015H0030)Fujian Provincial Universities Projects,China(No.JK2014042)
文摘The corn husks were usually discarded as useless materials,after alkali treatment from which the corn husk fiber was obtained. The corn husk fiber included half degumming corn husk bundle fiber and corn husk single fiber. This study found that corn husk single fiber was a kind of cellulose fiber,and was obtained with the fully alkali treatment technique,the process of which was NaOH concentration 0.15 g/mL,temperature at 80℃,and reaction time about 2.5 h. The morphologies of corn husk single fibers presented nature convolutions along with the fiber axis. They were closed at both ends,and they had a pentagram cavity and oval-shaped crosssection. They were flat shape,the fineness of the fibers was close to cotton fiber,and the mechanical properties of the fibers were similar to hemp fiber. So the corn husk fibers could be predicted that they could be used in textile industry because their properties were very close to cotton fiber or flax fiber.
文摘In our modern world, where conserving energy is highly valued, thermal insulation panels play a crucial role in reducing heat transfer between two spaces, surfaces, or materials. They are used to enhance the energy efficiency of various industrial applications by minimizing heat loss and temperature control. These panels function as silent protectors, aiding in reducing energy consumption and making things more sustainable and better for the environment. This is where composite materials come in;they are known for their lightweight nature, high strength-to-weight ratio, and excellent thermal insulation properties and have gained significant attention. Researchers are actively engaged in various studies aimed at enhancing these materials further. This research project focuses on the development of kaolin and glass fiber-reinforced composites for thermally insulating panels, to which natural strengthening materials like corn husk and bamboo fibers are added. The aim is to create cost-effective and efficient composite materials for thermal insulation applications by incorporating these components with a binder consisting of potassium silicate, hydroxide, and distilled water. This project involves conducting compression tests, bending tests, impact tests, thermal conductivity measurements, and microscopic analysis to evaluate the mechanical and thermal properties of the developed composites. The profound impact of these engineered composites on thermal insulation panels stands to revolutionize energy conservation efforts, offering a potent avenue to minimize heat loss and enhance overall energy efficiency across an array of industrial sectors.