期刊文献+
共找到323篇文章
< 1 2 17 >
每页显示 20 50 100
Effects of external temperature and dead volume on laboratory measurements of pore pressure and injected volume in a rock fracture
1
作者 Yinlin Ji Christian Kluge +1 位作者 Hannes Hofmann Guido Blöcher 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2022年第5期1461-1469,共9页
The accurate evaluation of pore pressure and injected volume is crucial for the laboratory characterization of hydromechanical responses of rock fractures. This study reports a series of laboratory experiments to syst... The accurate evaluation of pore pressure and injected volume is crucial for the laboratory characterization of hydromechanical responses of rock fractures. This study reports a series of laboratory experiments to systematically demonstrate the effects of external temperature and dead volume on laboratory measurements of pore pressure and injected volume in a rock fracture. We characterize the hydraulic aperture of the fracture as a function of effective normal stress using the exponential aperture model.This model is then employed to predict the pore pressure change and injected volume in the fracture without the influences of external temperature and dead volume. The external temperature changes in the cyclic loading test due to the Joule-Thompson effect for fluids. The effect of external temperature on pore pressure change in the fracture can be well explained by thermal pressurization of fluids. Our results also show that the external dead volume can significantly lower the pore pressure change in the fracture during the cyclic loading test under undrained conditions. The injected volume can also be substantially enlarged due to the external dead volume in a typical pore pressure system. Internal measurement of the pore pressure in the fracture using a fiber optic sensor cannot exclude the influences of external temperature and dead volume, primarily because of the good hydraulic communication between the fracture and pore pressure system. This study suggests that the effects of external temperature and dead volume on pore pressure response and injected volume should be evaluated for accurate laboratory characterization and inter-laboratory comparison. 展开更多
关键词 Fracture aperture Thermal pressurization External dead volume Pressure attenuation Injected volume Fiber optic sensor
下载PDF
Vibration Control of A Flexible Marine Riser System Subject to Input Dead Zone and Extraneous Disturbances 被引量:1
2
作者 ZHOU Li WANG Guo-rong WAN Min 《China Ocean Engineering》 SCIE EI CSCD 2024年第2期271-284,共14页
An observer-based adaptive backstepping boundary control is proposed for vibration control of flexible offshore riser systems with unknown nonlinear input dead zone and uncertain environmental disturbances.The control... An observer-based adaptive backstepping boundary control is proposed for vibration control of flexible offshore riser systems with unknown nonlinear input dead zone and uncertain environmental disturbances.The control algorithm can update the control law online through real-time data to make the controller adapt to the environment and improve the control precision.Specifically,based on the adaptive backstepping framework,virtual control laws and Lyapunov functions are designed for each subsystem.Three direction interference observers are designed to track the timevarying boundary disturbance.On this basis,the inverse of the dead zone and linear state transformation are used to compensate for the original system and eliminate the adverse effects of the dead zone.In addition,the stability of the closed-loop system is proven by Lyapunov stability theory.All the system states are bounded,and the vibration offset of the riser converges to a small area of the initial position.Finally,four examples of flexible marine risers are simulated in MATLAB to verify the effectiveness of the proposed controller. 展开更多
关键词 adaptive backstepping control disturbance observer flexible marine riser input dead zone vibration control
下载PDF
The “Dead Universe” Theory: Natural Separation of Galaxies Driven by the Remnants of a Supermassive Dead Universe
3
作者 Joel Almeida 《Natural Science》 2024年第6期65-101,共37页
This article explores the dead universe theory as a novel interpretation for the origin and evolution of the universe, suggesting that our cosmos may have originated from the remnants of a preceding universe. This per... This article explores the dead universe theory as a novel interpretation for the origin and evolution of the universe, suggesting that our cosmos may have originated from the remnants of a preceding universe. This perspective challenges the conventional Big Bang theory, particularly concerning dark matter, the expansion of the universe, and the interpretation of phenomena such as gravitational waves. 展开更多
关键词 dead Universe Theory Heat Death of the Universe Big Bang Theory Universe’s Ultimate Fate Universe Expansion Big Freeze Universe Cosmological Models End of Universe Theories Natural Galaxy Drift Future of the Universe Universe Cooling Down Cosmology and Entropy
下载PDF
结直肠癌组织和细胞中DEAD盒解旋酶10和Bystin样蛋白的表达及其临床意义
4
作者 涂艳娟 郑雪琴 +3 位作者 林欣怡 杨静波 邱燕如 许天文 《中国肿瘤生物治疗杂志》 CAS CSCD 北大核心 2023年第11期1009-1015,共7页
目的:探讨结直肠癌(CRC)组织及结肠癌细胞DLD-1中DEAD盒解旋酶10(DDX10)与Bystin-like(BYSL)的关系及其临床意义。方法:收集2017年3月至2018年3月间福建医科大学附属第二医院手术切除的78例CRC组织和配对癌旁组织,采用免疫组织化学Envis... 目的:探讨结直肠癌(CRC)组织及结肠癌细胞DLD-1中DEAD盒解旋酶10(DDX10)与Bystin-like(BYSL)的关系及其临床意义。方法:收集2017年3月至2018年3月间福建医科大学附属第二医院手术切除的78例CRC组织和配对癌旁组织,采用免疫组织化学Envision法检测癌组织和癌旁组织中DDX10和BYSL的表达水平,采用卡方检验、Pearson相关性分析和Kaplan-Meier法分别分析CRC组织中DDX10、BYSL蛋白表达与其患者的临床病例特征的关系,两者表达的相关性,以及与患者PFS和OS的关系。利用瞬时转染技术在DLD-1细胞中分别转染siDDX10和siNC,用qPCR法检测DLD-1细胞中敲减DDX10的表达对BYSL表达的影响,用CCK-8及Transwell实验验证DDX10及BYSL表达对结肠癌细胞增殖迁移及侵袭的影响。结果:免疫组织化学Envision法检测显示,与癌旁组织相比,DDX10和BYSL在CRC组织中呈高表达(73.08%和74.36%)。CRC组织中DDX10与BY肿瘤分期、是否发生淋巴结转移及复发等临床病例特征均有关联(均P<0.05)。Pearson相关分析结果显示,DDX10与BYSL的表达呈正相关(r=0.636,P<0.001)。Kaplan-Meier生存分析结果显示,DDX10与BYSL的高表达与患者更差的预后相关。Logistic回归分析发现,DDX10与BYSL均是CRC复发的独立风险因素。CCK-8法及Transwell实验检测结果显示,敲降DDX10的表达可使BYSL表达降低,且可抑制DLD-1细胞的增殖、迁移、侵袭能力(均P<0.05)。结论:DDX10和BYSL在CRC组织中呈高表达,两者表达水平呈正相关,且与患者更差的预后相关,是CRC复发的独立风险因素。可调控结直肠癌DLD-1细胞的增殖、迁移、侵袭能力。 展开更多
关键词 结直肠癌 dead盒解旋酶10 Bystin-like 复发 预后
下载PDF
Modelling the dead fuel moisture content in a grassland of Ergun City,China
5
作者 CHANG Chang CHANG Yu +1 位作者 GUO Meng HU Yuanman 《Journal of Arid Land》 SCIE CSCD 2023年第6期710-723,共14页
The dead fuel moisture content(DFMC)is the key driver leading to fire occurrence.Accurately estimating the DFMC could help identify locations facing fire risks,prioritise areas for fire monitoring,and facilitate timel... The dead fuel moisture content(DFMC)is the key driver leading to fire occurrence.Accurately estimating the DFMC could help identify locations facing fire risks,prioritise areas for fire monitoring,and facilitate timely deployment of fire-suppression resources.In this study,the DFMC and environmental variables,including air temperature,relative humidity,wind speed,solar radiation,rainfall,atmospheric pressure,soil temperature,and soil humidity,were simultaneously measured in a grassland of Ergun City,Inner Mongolia Autonomous Region of China in 2021.We chose three regression models,i.e.,random forest(RF)model,extreme gradient boosting(XGB)model,and boosted regression tree(BRT)model,to model the seasonal DFMC according to the data collected.To ensure accuracy,we added time-lag variables of 3 d to the models.The results showed that the RF model had the best fitting effect with an R2value of 0.847 and a prediction accuracy with a mean absolute error score of 4.764%among the three models.The accuracies of the models in spring and autumn were higher than those in the other two seasons.In addition,different seasons had different key influencing factors,and the degree of influence of these factors on the DFMC changed with time lags.Moreover,time-lag variables within 44 h clearly improved the fitting effect and prediction accuracy,indicating that environmental conditions within approximately 48 h greatly influence the DFMC.This study highlights the importance of considering 48 h time-lagged variables when predicting the DFMC of grassland fuels and mapping grassland fire risks based on the DFMC to help locate high-priority areas for grassland fire monitoring and prevention. 展开更多
关键词 dead fuel moisture content(DFMC) random forest(RF)model extreme gradient boosting(XGB)model boosted regression tree(BRT)model GRASSLAND Ergun City
下载PDF
Evaluation of Nutritional Value and Safety of Black Soldier Flies Fed on Dead Pig Meat and Bone Meal
6
作者 Tianxiang XUE Bo HONG +4 位作者 Jin XIANG Zhigang HE Xing TIAN Jinlong LI Xiaofei CHENG 《Agricultural Biotechnology》 2023年第6期35-38,92,共5页
[Objectives]This study was conducted to evaluate the nutritional value and safety of black soldier flies fed with dead pig meat and bone meal(DPMBM)and to explore the resource utilization of DPMBM.[Methods]The general... [Objectives]This study was conducted to evaluate the nutritional value and safety of black soldier flies fed with dead pig meat and bone meal(DPMBM)and to explore the resource utilization of DPMBM.[Methods]The general nutrient composition,amino acids,fatty acids and mineral elements of black soldier fly meal(BSLM)were detected and analyzed.[Results]The results showed that the contents of moisture,crude protein,crude fat and ash in BSLM were 3.42%,42.31%,34.04%and 5.40%,respectively.The contents of total amino acids(TAA),essential amino acids(∑EAA)and non-essential amino acids(∑NEAA)and umami amino acids(∑DAA),the EAA/TAA value and the EAA/NEAA value were 37.93%,13.08%,24.85%,13.43%,34.47%,52.61%,respectively.A total of eight kinds of saturated fatty acids and seven kinds of unsaturated fatty acids were detected,accounting for 63.65%and 32.67%of the total fatty acids.Among the major mineral elements,the content of Ca was the highest,followed by K,Mg and Na.Among the trace mineral elements,the content of Mn was the highest,followed by Fe,Zn,Cu,Ni,Cr,Cd,As,Pb,Se,Sn,Ti,Sb and Hg.The contents of heavy metal mineral elements in BSLM were far lower than the limits specified in Hygienical Standard for Feeds.[Conclusions]In conclusion,BSLM has high nutritional value and good safety of heavy metals,and thus great potential for development and utilization as a high quality dietary protein,fat and mineral elements source. 展开更多
关键词 dead pig carcass Meat and bone meal Black soldier fly Nutritional components Mineral elements
下载PDF
电影《Dead Poets Society》中对教育共情力的思考
7
作者 刘以诚 《教育进展》 2023年第11期8197-8202,共6页
共情力是个体在观察和理解他人情感的基础上,产生与观察对象相似的情感体验并表现出相应行为反应的一种能力。在教育过程中,家庭和学校对个体的教育最为直接,学生阶段是个体人生成长的重要时期,好的教育是需要共情力的。本文以美国影片... 共情力是个体在观察和理解他人情感的基础上,产生与观察对象相似的情感体验并表现出相应行为反应的一种能力。在教育过程中,家庭和学校对个体的教育最为直接,学生阶段是个体人生成长的重要时期,好的教育是需要共情力的。本文以美国影片《Dead Poets Society》为例,针对“共情教育”,启发家长、教师在教育过程中的共情能力对个体心理发展所具有的积极作用。提出加强教育者共情意识,家校合力,建立轻松和谐的交往氛围,促进教育者与受教育者之间关系融洽。 展开更多
关键词 共情力 dead Poets Society 教育思考
下载PDF
Chimeric ALT Perforator-Free Flap with Vastus Lateralis Muscle for the Obliteration of the Intrathoracic Dead Space Post-Pneumonectomy: A Case Report
8
作者 Nelson Oduor Ouma Daniel Odhiambo Otieno +1 位作者 Lee Njagi Munge Ferdinand Wanjala Nang’ole 《Modern Plastic Surgery》 2023年第3期63-73,共11页
Chest wall reconstruction is a surgical procedure aimed at restoring the integrity and function of the chest wall, which may be damaged due to trauma, cancer, infection, or congenital defects. The chest wall plays a v... Chest wall reconstruction is a surgical procedure aimed at restoring the integrity and function of the chest wall, which may be damaged due to trauma, cancer, infection, or congenital defects. The chest wall plays a vital role in protecting the thoracic organs, supporting the respiratory system, and maintaining the shape of the chest. Therefore, any defect or deformity of the chest wall can have significant functional and aesthetic consequences for the patient. The authors present a case report at Kenyatta National Hospital (KNH) of a dyspneic 47-year-old male patient with a right anterolateral chest wall defect post-pneumonectomy previously complicated by bronchopleural fistula. Past attempts at the chest wall reconstruction had utilized the ipsilateral latissimus dorsi muscle, pectoralis major muscle, and the omental pedicled flaps with limited success. A chimeric anterolateral thigh (ALT) perforator-free flap with vastus lateralis (VL) muscle was used to obliterate the post-pneumonectomy intrathoracic dead space and to provide a cutaneous paddle. This case report aims to show the versatility of the ALT flap for chest wall reconstruction to prevent the post-pneumonectomy syndrome associated with tracheal deviation, inspiratory stridor, and exertional dyspnea. In conclusion, chest wall reconstruction with obliteration of intrathoracic dead space post-pneumonectomy is challenging and needs careful planning and execution. 展开更多
关键词 Chest Wall Reconstruction Bronchopleural Fistula PNEUMONECTOMY Chimeric ALT Flap Intrathoracic dead Spacepost-Pneumonectomy
下载PDF
Dead Reckoning技术在网络游戏中的应用 被引量:3
9
作者 梁白鸥 陈雷霆 蔡洪斌 《计算机应用研究》 CSCD 北大核心 2007年第9期231-232,314,共3页
介绍了基于物理运动学算法的预测技术以及基于Cubic Splines算法的平滑处理技术,并给出了Dead Reckoning在网络游戏中应用的具体算法以及该算法的相应实例。
关键词 dead Reckoning 网络延迟 网络游戏 客户端预测 平滑处理
下载PDF
DEAD box家族蛋白与大肠癌 被引量:4
10
作者 沈翠翠 黄培 《世界华人消化杂志》 CAS 2016年第18期2811-2816,共6页
大肠癌是常见的消化系肿瘤之一,严重威胁着人类健康.DEAD box家族在RNA加工过程中发挥重要作用,能够利用水解ATP产生的能量参与前mRNA剪接、翻译起始、mRNA转运、核蛋白体生成、RNA降解以及线粒体和细胞核内RNA转录后加工等生物学过程.... 大肠癌是常见的消化系肿瘤之一,严重威胁着人类健康.DEAD box家族在RNA加工过程中发挥重要作用,能够利用水解ATP产生的能量参与前mRNA剪接、翻译起始、mRNA转运、核蛋白体生成、RNA降解以及线粒体和细胞核内RNA转录后加工等生物学过程.已有研究表明多种RNA螺旋酶在肿瘤中表达失调,部分RNA螺旋酶参与细胞分化、细胞周期、细胞凋亡、癌基因表达和肿瘤耐药基因表达的调控.本文就DEAD box对大肠癌生理、生化影响的研究进展作一简要综述. 展开更多
关键词 dead box家族 大肠癌 作用机制
下载PDF
恶性疟原虫abstrakt同源基因的克隆及DEAD盒家族蛋白的序列分析(英文) 被引量:1
11
作者 宋平 张竞男 +3 位作者 Pawan MALHOTRA Narendra TUTEJA Virender Singh CHAUHAN 李奎 《动物学报》 SCIE CAS CSCD 北大核心 2004年第3期420-430,共11页
DEAD盒蛋白家族的ATP依赖性的RNA解旋酶类参与细胞内几乎所有的RNA代谢过程 ,在几乎所有生物的细胞生长发育过程中扮演着众多不可或缺的角色。在本实验中 ,通过PCR和探针杂交相结合的筛选方法 ,筛选恶性疟原虫 (Plasmodiumfalciparum)... DEAD盒蛋白家族的ATP依赖性的RNA解旋酶类参与细胞内几乎所有的RNA代谢过程 ,在几乎所有生物的细胞生长发育过程中扮演着众多不可或缺的角色。在本实验中 ,通过PCR和探针杂交相结合的筛选方法 ,筛选恶性疟原虫 (Plasmodiumfalciparum)的基因组文库 ,克隆了FH1F———abstrakt同源基因的完整序列。通过搜索已经完成测序的恶性疟原虫基因组数据库 ,推测FH1F序列定位在第 5条染色体上。FH1F全长2 80 4bp,包含一个 1 1 6 1bp的完整阅读框 ,编码一个由 386个氨基酸组成的蛋白。对FH1F蛋白序列用BlastP进行搜索和分析以及用DNAStar与许多典型的DEAD盒蛋白序列进行比对分析 ,结果均提示FH1F蛋白应该是DEAD盒家族的一个Abstrakt蛋白。另一方面 ,用DNAStar对已知所有完整的DEAD盒蛋白进行详细的序列分析以及用Mega对这些序列进行系统发育研究的结果都显示 :DEAD盒家族的蛋白聚类成为若干不同的亚群 ;与DEAD盒蛋白的一般保守序列相比 ,Abstrakt,eIF 4A ,Vasa ,P6 8等不同亚群的DEAD盒蛋白在保守区具有各自不同的结构特征。本文对不同的DEAD盒蛋白的结构特征进行了总结并试图给出不同亚群分类上的结构标准 。 展开更多
关键词 恶性疟原虫 abstrakt 同源基因 克隆 dead 序列分析 RNA解旋酶
下载PDF
甲醛固定对Live/Dead BacLight Bacterial Viability Kit死活细菌染液荧光显微计数海洋细菌的影响 被引量:2
12
作者 张瑜斌 崔焱芸 +1 位作者 郑运 孙省利 《生态科学》 CSCD 北大核心 2013年第5期636-641,共6页
利用Live/Dead BacLight Bacterial Viability Kit死活细菌染液对采自湛江东海大堤海水、沉积物细菌和大型海藻拟刚毛藻(Cladophoropsis zollingeri)内生细菌数量进行了甲醛固定处理前后的荧光显微计数对比分析。结果表明,新鲜样品(不... 利用Live/Dead BacLight Bacterial Viability Kit死活细菌染液对采自湛江东海大堤海水、沉积物细菌和大型海藻拟刚毛藻(Cladophoropsis zollingeri)内生细菌数量进行了甲醛固定处理前后的荧光显微计数对比分析。结果表明,新鲜样品(不加甲醛固定)、甲醛刚固定样品、甲醛固定1周样品和甲醛固定2周样品中海洋细菌数量差异不显著(p>0.05)。甲醛固定对Live/Dead BacLight Bacterial Viability Kit死活细菌染液荧光显微计数海洋细菌数量无显著影响,固定后的样品可在2周内完成计数。 展开更多
关键词 甲醛固定 LIVE dead BacLight Bacterial Viability Kit染液 细菌荧光显微计数
下载PDF
DEAD盒多肽43在肺腺癌组织中的表达 被引量:1
13
作者 崔黎 崔莹 +1 位作者 赵娜 陈奎生 《肿瘤基础与临床》 2020年第4期294-297,共4页
目的探讨DEAD盒多肽43(DDX43)在肺腺癌组织中的表达。方法应用免疫组化、荧光原位杂交技术观察DDX43在正常肺组织、肺泡上皮非典型腺瘤样增生及肺腺癌组织中表达情况,并对其数据进行比较分析。结果正常肺组织、肺泡上皮非典型腺瘤样增... 目的探讨DEAD盒多肽43(DDX43)在肺腺癌组织中的表达。方法应用免疫组化、荧光原位杂交技术观察DDX43在正常肺组织、肺泡上皮非典型腺瘤样增生及肺腺癌组织中表达情况,并对其数据进行比较分析。结果正常肺组织、肺泡上皮非典型腺瘤样增生、肺腺癌组织中DDX43蛋白的阳性表达率分别为0.00%、38.71%、88.71%,总体比较差异有统计学意义(χ^2=63.150,P<0.001)。正常肺组织、肺泡上皮非典型腺瘤样增生、肺腺癌组织中DDX43 mRNA的阳性表达率分别为8.06%、38.71%、82.26%,总体比较差异有统计学意义(χ^2=53.124,P<0.001)。肺腺癌组织中DDX43蛋白和mRNA的表达均与其有无淋巴结转移、胸膜侵犯、脉管侵犯有关(蛋白χ^2=3.214,P=0.040;χ^2=14.230,P=0.001;χ^2=10.400,P=0.001;mRNAχ^2=4.590,P=0.030;χ^2=6.223,P=0.008;χ^2=10.400,P=0.001)。结论肺腺癌组织中DDX43高表达,DDX43蛋白、mRNA表达与肺腺癌的发生、发展、浸润、转移有关。 展开更多
关键词 dead盒多肽43 肺腺癌 免疫组化 荧光原位杂交
下载PDF
肝细胞肝癌中DDX24、TRIP12表达与上皮间质转化及临床预后的关系
14
作者 井丽君 张敏 +1 位作者 魏雪 唐玉彬 《疑难病杂志》 CAS 2024年第6期646-652,共7页
目的探讨肝细胞肝癌(HCC)中DEAD盒解旋酶24(DDX24)、甲状腺激素受体相互作用因子12(TRIP12)表达,分析两者与上皮间质转化(EMT)及临床预后的关系。方法回顾性选取2019年2月—2021年1月中国人民解放军联勤保障部队第九四〇医院肝胆外科手... 目的探讨肝细胞肝癌(HCC)中DEAD盒解旋酶24(DDX24)、甲状腺激素受体相互作用因子12(TRIP12)表达,分析两者与上皮间质转化(EMT)及临床预后的关系。方法回顾性选取2019年2月—2021年1月中国人民解放军联勤保障部队第九四〇医院肝胆外科手术治疗的HCC患者96例。免疫组化检测癌组织和癌旁组织DDX24、TRIP12蛋白表达;采用实时荧光定量PCR检测DDX24、TRIP12和EMT指标[E-钙黏素(E-cad)、N-钙黏素(N-cad)及TWIST]表达;Pearson相关分析DDX24 mRNA、TRIP12 mRNA和EMT指标的相关性;绘制Kaplan-Meier曲线,Log-Rank检验分析DDX24 mRNA、TRIP12 mRNA高表达组和低表达组HCC患者预后的差异;多因素Cox比例风险模型分析影响HCC患者死亡预后的独立因素。结果HCC癌组织中DDX24、TRIP12阳性率显著高于癌旁组织(χ^(2)/P=109.714/<0.001,108.755/<0.001);与癌旁组织比较,HCC癌组织中DDX24、TRIP12、N-cad、TWIST mRNA表达较高,E-cad mRNA表达较低,差异均有统计学意义(t/P=35.810/<0.001,48.036/<0.001,25.015/<0.001,48.482/<0.001,38.069/<0.001)。HCC癌组织中DDX24 mRNA与TRIP12 mRNA表达呈正相关(r=0.701,P<0.001);DDX24 mRNA、TRIP12 mRNA均与N-cad mRNA、TWIST mRNA呈正相关,与E-cad mRNA呈负相关(r/P=0.723/<0.001,0.661/<0.001,0.706/<0.001,0.745/<0.001,-0.694/<0.001,-0.609/<0.001)。低分化程度、CNLC分期Ⅱ~Ⅲ期和有血管侵犯HCC患者癌组织中DDX24、TRIP12 mRNA高于高中分化程度、CNLC分期Ⅰ期和无血管侵犯者(DDX24:t/P=10.348/<0.001,18.474/<0.001,6.302/<0.001;TRIP12:t/P=25.661/<0.001,36.396/<0.001,14.928/<0.001)。DDX24 mRNA高表达组和低表达组3年总生存率分别为39.13%(18/46)、64.00%(32/50),2组生存曲线总体比较,差异具有统计学意义(Log rankχ^(2)=7.491,P=0.006);TRIP12 mRNA高表达组和低表达组3年总生存率分别为36.17%(17/47)、67.35%(33/49),2组生存曲线总体比较,差异有统计学意义(Log rankχ^(2)=8.146,P=0.004)。CNLC分期Ⅱ~Ⅲ期、低分化程度、血管侵犯、DDX24 mRNA高表达、TRIP12 mRNA高表达是影响HCC患者死亡预后的危险因素[HR(95%CI)=1.611(1.175~2.209),1.768(1.238~2.526),1.464(1.089~1.968),1.859(1.330~2.599),1.775(1.275~2.473)]。结论HCC癌组织中DDX24、TRIP12表达上调,与N-cad、TWIST呈正相关,与E-cad呈负相关,DDX24、TRIP12可能通过促进HCC肿瘤EMT的发生,促进HCC肿瘤的恶性进展,两者是影响HCC患者死亡预后的独立危险因素。 展开更多
关键词 肝细胞肝癌 dead盒解旋酶24 甲状腺激素受体相互作用因子12 上皮间质转化 临床预后
下载PDF
转录因子HNF1A、HNF4A和FOXA2调节肝细胞蛋白质N-糖基化
15
作者 Vedrana Vicic Bockor Nika Foglar +7 位作者 Goran Josipovic Marija Klasic Ana Vujic Branimir Plavsa Toma Keser Samira Smajlovic Aleksandar Vojta Vlatka Zoldos 《Engineering》 SCIE EI CAS CSCD 2024年第1期57-68,共12页
Hepatocyte nuclear factor 1 alpha(HNF1A),hepatocyte nuclear factor 4 alpha(HNF4A),and forkhead box protein A2(FOXA2)are key transcription factors that regulate a complex gene network in the liver,cre-ating a regulator... Hepatocyte nuclear factor 1 alpha(HNF1A),hepatocyte nuclear factor 4 alpha(HNF4A),and forkhead box protein A2(FOXA2)are key transcription factors that regulate a complex gene network in the liver,cre-ating a regulatory transcriptional loop.The Encode and ChIP-Atlas databases identify the recognition sites of these transcription factors in many glycosyltransferase genes.Our in silico analysis of HNF1A,HNF4A.and FOXA2 binding to the ten candidate glyco-genes studied in this work confirms a significant enrich-ment of these transcription factors specifically in the liver.Our previous studies identified HNF1A as a master regulator of fucosylation,glycan branching,and galactosylation of plasma glycoproteins.Here,we aimed to functionally validate the role of the three transcription factors on downstream glyco-gene transcriptional expression and the possible effect on glycan phenotype.We used the state-of-the-art clus-tered regularly interspaced short palindromic repeats/dead Cas9(CRISPR/dCas9)molecular tool for the downregulation of the HNF1A,HNF4A,and FOXA2 genes in HepG2 cells-a human liver cancer cell line.The results show that the downregulation of all three genes individually and in pairs affects the transcrip-tional activity of many glyco-genes,although downregulation of glyco-genes was not always followed by an unambiguous change in the corresponding glycan structures.The effect is better seen as an overall change in the total HepG2 N-glycome,primarily due to the extension of biantennary glycans.We propose an alternative way to evaluate the N-glycome composition via estimating the overall complexity of the glycome by quantifying the number of monomers in each glycan structure.We also propose a model showing feedback loops with the mutual activation of HNF1A-FOXA2 and HNF4A-FOXA2 affecting glyco-genes and protein glycosylation in HepG2 cells. 展开更多
关键词 Clustered regularly interspaced short palindromic repeats/dead Cas9(CRISPR/dCas9) EPIGENETICS Hepatocyte nuclear factor 1 alpha(HNF1A) Hepatocyte nuclear factor 4 alpha(HNF4A) Forkhead box protein A2(FOXA2) N-GLYCOSYLATION HepG2 cells
下载PDF
DEAD盒多肽43小干扰RNA对司美替尼抑制人肺腺癌A549细胞增殖和诱导其凋亡作用的影响
16
作者 崔黎 崔莹 +1 位作者 赵娜 陈奎生 《肿瘤基础与临床》 2021年第1期1-5,共5页
目的探讨DEAD盒多肽43(DDX43)小干扰RNA(siRNA)对司美替尼抑制人肺腺癌A549细胞增殖和诱导其凋亡作用的影响。方法培养A549细胞,并分为对照组、司美替尼组和司美替尼+DDX43 siRNA组,运用细胞免疫组化和原位杂交的方法分别检测各组A549... 目的探讨DEAD盒多肽43(DDX43)小干扰RNA(siRNA)对司美替尼抑制人肺腺癌A549细胞增殖和诱导其凋亡作用的影响。方法培养A549细胞,并分为对照组、司美替尼组和司美替尼+DDX43 siRNA组,运用细胞免疫组化和原位杂交的方法分别检测各组A549细胞中DDX43蛋白和mRNA的表达情况,采用MTT法检测各组细胞的增殖情况,采用TUNEL法检测各组细胞的凋亡情况。结果对照组、司美替尼组、司美替尼+DDX43 siRNA组DDX43蛋白相对表达量分别为138.20±17.72、79.95±8.96、36.16±6.31,总体比较差异有统计学意义(F=66.72,P=0.02);表达水平依次降低,且两两比较差异均有统计学意义(P均<0.05)。对照组、司美替尼组、司美替尼+DDX43 siRNA组DDX43 mRNA相对表达量分别为266.20±15.07、164.95±8.96、71.16±6.31,总体比较差异有统计学意义(F=125.35,P=0.01);表达水平依次降低,且两两比较差异均有统计学意义(P均<0.05)。对照组、司美替尼组、司美替尼+DDX43 siRNA组A549细胞凋亡指数分别为(3.75±0.55)%、(11.72±1.06)%、(19.98±1.21)%,总体比较差异有统计学意义(F=12.31,P=0.04);凋亡指数依次升高,且两两比较差异均有统计学意义(P均<0.05)。对照组、司美替尼组、司美替尼+DDX43 siRNA组A549细胞生长抑制率分别为(2.11±0.13)%、(14.91±0.29)%、(27.14±0.58)%,总体比较差异有统计学意义(F=15.23,P=0.03);生长抑制率依次升高,且两两比较差异均有统计学意义(P均<0.05)。结论DDX43 siRNA联合司美替尼可抑制A549细胞增殖,促进其凋亡。 展开更多
关键词 dead盒多肽43 小干扰RNA 肺腺癌
下载PDF
The Need for International Cooperation between Israel and Other Middle East Nations to Protect the Jordan River Landscape
17
作者 Kenneth Ray Olson 《Open Journal of Soil Science》 2024年第5期283-307,共25页
The Jordan River, also referred to as Nahr Al Sharieat in Arabic, is a long river in the Middle East that flows from north to south through the Sea of Galilee to the Dead Sea. The Jordan River is bordered by the Golan... The Jordan River, also referred to as Nahr Al Sharieat in Arabic, is a long river in the Middle East that flows from north to south through the Sea of Galilee to the Dead Sea. The Jordan River is bordered by the Golan Heights and the Hashemite Kingdom of Jordan in the east and by Israel and the Palestinian West Bank (Judea and Samaria) in the west. Soil tunnels, including those in the Jordan River watershed and on the Israel, Syria, and Lebanon borders, have a long history of use for warfare, as invasion pathways, smuggling, and storage of rockets, missiles and ordnance and are causes of serious political tension between the countries. Soil tunnel construction and destruction often has adverse environmental and human health impacts in the Jordan River landscape. Due to agricultural wastes, the discharge of untreated sewage, and diversion of saline springs into the river water there has been serious deterioration in the water quality in the lower courses of the Jordan River. The primary objective of this research is to encourage the development of a multi-country clean-up, mitigation, and protection plan for the Jordan River. 展开更多
关键词 Jordan River JORDAN Israel Lebanon SYRIA dead Sea Sea of Galilee Six-Day War Soil Tunnels
下载PDF
dead不“死”,live不“活”
18
作者 王守芳 《大学英语》 2003年第8期35-35,共1页
我们都知道,dead的意思是“死的”,形容词live的意思是“活的”。但它们的意思决不局限于此,同名词结合后能产生许多新义,若稍不注意便会误译,因此要特别注意。
关键词 dead 1ive 形容词 误译 英语 名词 翻译
下载PDF
Identification of quantitative trait loci for the dead leaf rate and the seedling dead rate under alkaline stress in rice 被引量:8
19
作者 Dongling Qi Guizhen Guo +6 位作者 Myung-chul Lee Junguo Zhang Guilan Cao Sanyuan Zhang Seok-cheol Suh Qingyang Zhou Longzhi Han 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2008年第5期299-305,共7页
The quantitative trait loci (QTLs) for the dead leaf rate (DLR) and the dead seedling rate (DSR) at the different rice growing periods after transplanting under alkaline stress were identified using an F2:3 pop... The quantitative trait loci (QTLs) for the dead leaf rate (DLR) and the dead seedling rate (DSR) at the different rice growing periods after transplanting under alkaline stress were identified using an F2:3 population, which included 200 individuals and lines derived from a cross between two japonica rice cultivars Gaochan 106 and Changbai 9 with microsatellite markers. The DLR detected at 20 days to 62 days after transplanting under alkaline stress showed continuous normal or near normal distributions in F3 lines, which was the quantitative trait controlled by multiple genes. The DSR showed a continuous distribution with 3 or 4 peaks and was the quantitative trait controlled by main and multiple genes when rice was grown for 62 days after transplanting under alkaline stress. Thirteen QTLs associated with DLR were detected at 20 days to 62 days after transplanting under alkaline stress. Among these, qDLR9-2 located in RM5786-RM160 on chromosome 9 was detected at 34 days, 41 days, 48 days, 55 days, and 62 days, respectively; qDLR4 located in RM3524-RM3866 on chromosome 4 was detected at 34 days, 41 days, and 48 days, respectively; qDLR7-1 located in RM3859-RM320 on chromosome 7 was detected at 20 days and 27 days; and qDLR6-2 in RM1340-RM5957 on chromosome 6 was detected at 55 days and 62 days, respectively. The alleles of both qDLR9-2 and qDLR4 were derived from alkaline sensitive parent "Gaochanl06". The alleles of both qDLR7-1 and qDLR6-2 were from alkaline tolerant parent Changbai 9. These gene actions showed dominance and over dominance primarily. Six QTLs associated with DSR were detected at 62 days after transplanting under alkaline stress. Among these, qDSR6-2 and qDSR8 were located in RM1340-RM5957 on chromosome 6 and in RM3752-RM404 on chromosome 8, respectively, which were associated with DSR and accounted for 20.32% and 18.86% of the observed phenotypic variation, respectively; qDSR11-2 and qDSR11-3 were located in RM536-RM479 and RM2596-RM286 on chromosome 11, respectively, which were associated with DSR explaining 25.85% and 15.41% of the observed phenotypic variation, respectively. The marker flanking distances of these QTLs were quite far except that of qDSR6-2, which should be researched further. 展开更多
关键词 RICE alkaline stress dead leaf rate dead seedling rate microsatellite marker quantitative trait locus (QTL)
下载PDF
一个水稻DEAD盒RNA解旋酶基因OsBIRH1的克隆鉴定及其蛋白纯化
20
作者 宋莉芸 陈洁 +1 位作者 郑重 宋凤鸣 《浙江农业学报》 CSCD 2007年第4期259-263,共5页
克隆鉴定了一个水稻DEAD盒RNA解旋酶基因OsBIRH1。OsBIRH1基因全长cDNA由2167个核苷酸组成,包含1个长1926个核苷酸的开放阅读框,编码1个由641个氨基酸组成的蛋白,预测分子量为70.8kD。OsBIRHI基因位于水稻第3号染色体上,由8个外显... 克隆鉴定了一个水稻DEAD盒RNA解旋酶基因OsBIRH1。OsBIRH1基因全长cDNA由2167个核苷酸组成,包含1个长1926个核苷酸的开放阅读框,编码1个由641个氨基酸组成的蛋白,预测分子量为70.8kD。OsBIRHI基因位于水稻第3号染色体上,由8个外显子和7个内含子组成。预测的OsBIRH1蛋白包含DEAD盒解旋酶所特有的保守DEAD和HELICc结构域以及保守模体结构。系统进化树分析表明,OsBIRH1与拟南芥和人DEAD盒解旋酶有较高的同源性,氨基酸序列同一性为47%~54%。原核表达了OsBIRH1基因,纯化获得重组OsBIRH1融合蛋白,为OsBIRH1生化与生物学功能研究提供科学依据。 展开更多
关键词 水稻 dead盒解旋酶 OsBIRH1
下载PDF
上一页 1 2 17 下一页 到第
使用帮助 返回顶部