The accurate evaluation of pore pressure and injected volume is crucial for the laboratory characterization of hydromechanical responses of rock fractures. This study reports a series of laboratory experiments to syst...The accurate evaluation of pore pressure and injected volume is crucial for the laboratory characterization of hydromechanical responses of rock fractures. This study reports a series of laboratory experiments to systematically demonstrate the effects of external temperature and dead volume on laboratory measurements of pore pressure and injected volume in a rock fracture. We characterize the hydraulic aperture of the fracture as a function of effective normal stress using the exponential aperture model.This model is then employed to predict the pore pressure change and injected volume in the fracture without the influences of external temperature and dead volume. The external temperature changes in the cyclic loading test due to the Joule-Thompson effect for fluids. The effect of external temperature on pore pressure change in the fracture can be well explained by thermal pressurization of fluids. Our results also show that the external dead volume can significantly lower the pore pressure change in the fracture during the cyclic loading test under undrained conditions. The injected volume can also be substantially enlarged due to the external dead volume in a typical pore pressure system. Internal measurement of the pore pressure in the fracture using a fiber optic sensor cannot exclude the influences of external temperature and dead volume, primarily because of the good hydraulic communication between the fracture and pore pressure system. This study suggests that the effects of external temperature and dead volume on pore pressure response and injected volume should be evaluated for accurate laboratory characterization and inter-laboratory comparison.展开更多
An observer-based adaptive backstepping boundary control is proposed for vibration control of flexible offshore riser systems with unknown nonlinear input dead zone and uncertain environmental disturbances.The control...An observer-based adaptive backstepping boundary control is proposed for vibration control of flexible offshore riser systems with unknown nonlinear input dead zone and uncertain environmental disturbances.The control algorithm can update the control law online through real-time data to make the controller adapt to the environment and improve the control precision.Specifically,based on the adaptive backstepping framework,virtual control laws and Lyapunov functions are designed for each subsystem.Three direction interference observers are designed to track the timevarying boundary disturbance.On this basis,the inverse of the dead zone and linear state transformation are used to compensate for the original system and eliminate the adverse effects of the dead zone.In addition,the stability of the closed-loop system is proven by Lyapunov stability theory.All the system states are bounded,and the vibration offset of the riser converges to a small area of the initial position.Finally,four examples of flexible marine risers are simulated in MATLAB to verify the effectiveness of the proposed controller.展开更多
This article explores the dead universe theory as a novel interpretation for the origin and evolution of the universe, suggesting that our cosmos may have originated from the remnants of a preceding universe. This per...This article explores the dead universe theory as a novel interpretation for the origin and evolution of the universe, suggesting that our cosmos may have originated from the remnants of a preceding universe. This perspective challenges the conventional Big Bang theory, particularly concerning dark matter, the expansion of the universe, and the interpretation of phenomena such as gravitational waves.展开更多
The dead fuel moisture content(DFMC)is the key driver leading to fire occurrence.Accurately estimating the DFMC could help identify locations facing fire risks,prioritise areas for fire monitoring,and facilitate timel...The dead fuel moisture content(DFMC)is the key driver leading to fire occurrence.Accurately estimating the DFMC could help identify locations facing fire risks,prioritise areas for fire monitoring,and facilitate timely deployment of fire-suppression resources.In this study,the DFMC and environmental variables,including air temperature,relative humidity,wind speed,solar radiation,rainfall,atmospheric pressure,soil temperature,and soil humidity,were simultaneously measured in a grassland of Ergun City,Inner Mongolia Autonomous Region of China in 2021.We chose three regression models,i.e.,random forest(RF)model,extreme gradient boosting(XGB)model,and boosted regression tree(BRT)model,to model the seasonal DFMC according to the data collected.To ensure accuracy,we added time-lag variables of 3 d to the models.The results showed that the RF model had the best fitting effect with an R2value of 0.847 and a prediction accuracy with a mean absolute error score of 4.764%among the three models.The accuracies of the models in spring and autumn were higher than those in the other two seasons.In addition,different seasons had different key influencing factors,and the degree of influence of these factors on the DFMC changed with time lags.Moreover,time-lag variables within 44 h clearly improved the fitting effect and prediction accuracy,indicating that environmental conditions within approximately 48 h greatly influence the DFMC.This study highlights the importance of considering 48 h time-lagged variables when predicting the DFMC of grassland fuels and mapping grassland fire risks based on the DFMC to help locate high-priority areas for grassland fire monitoring and prevention.展开更多
[Objectives]This study was conducted to evaluate the nutritional value and safety of black soldier flies fed with dead pig meat and bone meal(DPMBM)and to explore the resource utilization of DPMBM.[Methods]The general...[Objectives]This study was conducted to evaluate the nutritional value and safety of black soldier flies fed with dead pig meat and bone meal(DPMBM)and to explore the resource utilization of DPMBM.[Methods]The general nutrient composition,amino acids,fatty acids and mineral elements of black soldier fly meal(BSLM)were detected and analyzed.[Results]The results showed that the contents of moisture,crude protein,crude fat and ash in BSLM were 3.42%,42.31%,34.04%and 5.40%,respectively.The contents of total amino acids(TAA),essential amino acids(∑EAA)and non-essential amino acids(∑NEAA)and umami amino acids(∑DAA),the EAA/TAA value and the EAA/NEAA value were 37.93%,13.08%,24.85%,13.43%,34.47%,52.61%,respectively.A total of eight kinds of saturated fatty acids and seven kinds of unsaturated fatty acids were detected,accounting for 63.65%and 32.67%of the total fatty acids.Among the major mineral elements,the content of Ca was the highest,followed by K,Mg and Na.Among the trace mineral elements,the content of Mn was the highest,followed by Fe,Zn,Cu,Ni,Cr,Cd,As,Pb,Se,Sn,Ti,Sb and Hg.The contents of heavy metal mineral elements in BSLM were far lower than the limits specified in Hygienical Standard for Feeds.[Conclusions]In conclusion,BSLM has high nutritional value and good safety of heavy metals,and thus great potential for development and utilization as a high quality dietary protein,fat and mineral elements source.展开更多
Chest wall reconstruction is a surgical procedure aimed at restoring the integrity and function of the chest wall, which may be damaged due to trauma, cancer, infection, or congenital defects. The chest wall plays a v...Chest wall reconstruction is a surgical procedure aimed at restoring the integrity and function of the chest wall, which may be damaged due to trauma, cancer, infection, or congenital defects. The chest wall plays a vital role in protecting the thoracic organs, supporting the respiratory system, and maintaining the shape of the chest. Therefore, any defect or deformity of the chest wall can have significant functional and aesthetic consequences for the patient. The authors present a case report at Kenyatta National Hospital (KNH) of a dyspneic 47-year-old male patient with a right anterolateral chest wall defect post-pneumonectomy previously complicated by bronchopleural fistula. Past attempts at the chest wall reconstruction had utilized the ipsilateral latissimus dorsi muscle, pectoralis major muscle, and the omental pedicled flaps with limited success. A chimeric anterolateral thigh (ALT) perforator-free flap with vastus lateralis (VL) muscle was used to obliterate the post-pneumonectomy intrathoracic dead space and to provide a cutaneous paddle. This case report aims to show the versatility of the ALT flap for chest wall reconstruction to prevent the post-pneumonectomy syndrome associated with tracheal deviation, inspiratory stridor, and exertional dyspnea. In conclusion, chest wall reconstruction with obliteration of intrathoracic dead space post-pneumonectomy is challenging and needs careful planning and execution.展开更多
Hepatocyte nuclear factor 1 alpha(HNF1A),hepatocyte nuclear factor 4 alpha(HNF4A),and forkhead box protein A2(FOXA2)are key transcription factors that regulate a complex gene network in the liver,cre-ating a regulator...Hepatocyte nuclear factor 1 alpha(HNF1A),hepatocyte nuclear factor 4 alpha(HNF4A),and forkhead box protein A2(FOXA2)are key transcription factors that regulate a complex gene network in the liver,cre-ating a regulatory transcriptional loop.The Encode and ChIP-Atlas databases identify the recognition sites of these transcription factors in many glycosyltransferase genes.Our in silico analysis of HNF1A,HNF4A.and FOXA2 binding to the ten candidate glyco-genes studied in this work confirms a significant enrich-ment of these transcription factors specifically in the liver.Our previous studies identified HNF1A as a master regulator of fucosylation,glycan branching,and galactosylation of plasma glycoproteins.Here,we aimed to functionally validate the role of the three transcription factors on downstream glyco-gene transcriptional expression and the possible effect on glycan phenotype.We used the state-of-the-art clus-tered regularly interspaced short palindromic repeats/dead Cas9(CRISPR/dCas9)molecular tool for the downregulation of the HNF1A,HNF4A,and FOXA2 genes in HepG2 cells-a human liver cancer cell line.The results show that the downregulation of all three genes individually and in pairs affects the transcrip-tional activity of many glyco-genes,although downregulation of glyco-genes was not always followed by an unambiguous change in the corresponding glycan structures.The effect is better seen as an overall change in the total HepG2 N-glycome,primarily due to the extension of biantennary glycans.We propose an alternative way to evaluate the N-glycome composition via estimating the overall complexity of the glycome by quantifying the number of monomers in each glycan structure.We also propose a model showing feedback loops with the mutual activation of HNF1A-FOXA2 and HNF4A-FOXA2 affecting glyco-genes and protein glycosylation in HepG2 cells.展开更多
The Jordan River, also referred to as Nahr Al Sharieat in Arabic, is a long river in the Middle East that flows from north to south through the Sea of Galilee to the Dead Sea. The Jordan River is bordered by the Golan...The Jordan River, also referred to as Nahr Al Sharieat in Arabic, is a long river in the Middle East that flows from north to south through the Sea of Galilee to the Dead Sea. The Jordan River is bordered by the Golan Heights and the Hashemite Kingdom of Jordan in the east and by Israel and the Palestinian West Bank (Judea and Samaria) in the west. Soil tunnels, including those in the Jordan River watershed and on the Israel, Syria, and Lebanon borders, have a long history of use for warfare, as invasion pathways, smuggling, and storage of rockets, missiles and ordnance and are causes of serious political tension between the countries. Soil tunnel construction and destruction often has adverse environmental and human health impacts in the Jordan River landscape. Due to agricultural wastes, the discharge of untreated sewage, and diversion of saline springs into the river water there has been serious deterioration in the water quality in the lower courses of the Jordan River. The primary objective of this research is to encourage the development of a multi-country clean-up, mitigation, and protection plan for the Jordan River.展开更多
The quantitative trait loci (QTLs) for the dead leaf rate (DLR) and the dead seedling rate (DSR) at the different rice growing periods after transplanting under alkaline stress were identified using an F2:3 pop...The quantitative trait loci (QTLs) for the dead leaf rate (DLR) and the dead seedling rate (DSR) at the different rice growing periods after transplanting under alkaline stress were identified using an F2:3 population, which included 200 individuals and lines derived from a cross between two japonica rice cultivars Gaochan 106 and Changbai 9 with microsatellite markers. The DLR detected at 20 days to 62 days after transplanting under alkaline stress showed continuous normal or near normal distributions in F3 lines, which was the quantitative trait controlled by multiple genes. The DSR showed a continuous distribution with 3 or 4 peaks and was the quantitative trait controlled by main and multiple genes when rice was grown for 62 days after transplanting under alkaline stress. Thirteen QTLs associated with DLR were detected at 20 days to 62 days after transplanting under alkaline stress. Among these, qDLR9-2 located in RM5786-RM160 on chromosome 9 was detected at 34 days, 41 days, 48 days, 55 days, and 62 days, respectively; qDLR4 located in RM3524-RM3866 on chromosome 4 was detected at 34 days, 41 days, and 48 days, respectively; qDLR7-1 located in RM3859-RM320 on chromosome 7 was detected at 20 days and 27 days; and qDLR6-2 in RM1340-RM5957 on chromosome 6 was detected at 55 days and 62 days, respectively. The alleles of both qDLR9-2 and qDLR4 were derived from alkaline sensitive parent "Gaochanl06". The alleles of both qDLR7-1 and qDLR6-2 were from alkaline tolerant parent Changbai 9. These gene actions showed dominance and over dominance primarily. Six QTLs associated with DSR were detected at 62 days after transplanting under alkaline stress. Among these, qDSR6-2 and qDSR8 were located in RM1340-RM5957 on chromosome 6 and in RM3752-RM404 on chromosome 8, respectively, which were associated with DSR and accounted for 20.32% and 18.86% of the observed phenotypic variation, respectively; qDSR11-2 and qDSR11-3 were located in RM536-RM479 and RM2596-RM286 on chromosome 11, respectively, which were associated with DSR explaining 25.85% and 15.41% of the observed phenotypic variation, respectively. The marker flanking distances of these QTLs were quite far except that of qDSR6-2, which should be researched further.展开更多
基金supported by the Research Fund of the State Key Laboratory of Coal Resources and Safe Mining,China University of Mining and Technology,China (Grant No. SKLCRSM21KF002)supported by the Initiative and Networking Fund of Helmholtz Association (Germany) for the Helmholtz Young Investigator Group ARES (Contract No. VH-NG-1516)。
文摘The accurate evaluation of pore pressure and injected volume is crucial for the laboratory characterization of hydromechanical responses of rock fractures. This study reports a series of laboratory experiments to systematically demonstrate the effects of external temperature and dead volume on laboratory measurements of pore pressure and injected volume in a rock fracture. We characterize the hydraulic aperture of the fracture as a function of effective normal stress using the exponential aperture model.This model is then employed to predict the pore pressure change and injected volume in the fracture without the influences of external temperature and dead volume. The external temperature changes in the cyclic loading test due to the Joule-Thompson effect for fluids. The effect of external temperature on pore pressure change in the fracture can be well explained by thermal pressurization of fluids. Our results also show that the external dead volume can significantly lower the pore pressure change in the fracture during the cyclic loading test under undrained conditions. The injected volume can also be substantially enlarged due to the external dead volume in a typical pore pressure system. Internal measurement of the pore pressure in the fracture using a fiber optic sensor cannot exclude the influences of external temperature and dead volume, primarily because of the good hydraulic communication between the fracture and pore pressure system. This study suggests that the effects of external temperature and dead volume on pore pressure response and injected volume should be evaluated for accurate laboratory characterization and inter-laboratory comparison.
基金financially supported by the Sichuan Science and Technology Program(Grant No.2023NSFSC1980)。
文摘An observer-based adaptive backstepping boundary control is proposed for vibration control of flexible offshore riser systems with unknown nonlinear input dead zone and uncertain environmental disturbances.The control algorithm can update the control law online through real-time data to make the controller adapt to the environment and improve the control precision.Specifically,based on the adaptive backstepping framework,virtual control laws and Lyapunov functions are designed for each subsystem.Three direction interference observers are designed to track the timevarying boundary disturbance.On this basis,the inverse of the dead zone and linear state transformation are used to compensate for the original system and eliminate the adverse effects of the dead zone.In addition,the stability of the closed-loop system is proven by Lyapunov stability theory.All the system states are bounded,and the vibration offset of the riser converges to a small area of the initial position.Finally,four examples of flexible marine risers are simulated in MATLAB to verify the effectiveness of the proposed controller.
文摘This article explores the dead universe theory as a novel interpretation for the origin and evolution of the universe, suggesting that our cosmos may have originated from the remnants of a preceding universe. This perspective challenges the conventional Big Bang theory, particularly concerning dark matter, the expansion of the universe, and the interpretation of phenomena such as gravitational waves.
基金funded by the National Key Research and Development Program of China Strategic International Cooperation in Science and Technology Innovation Program (2018YFE0207800)the National Natural Science Foundation of China (31971483)。
文摘The dead fuel moisture content(DFMC)is the key driver leading to fire occurrence.Accurately estimating the DFMC could help identify locations facing fire risks,prioritise areas for fire monitoring,and facilitate timely deployment of fire-suppression resources.In this study,the DFMC and environmental variables,including air temperature,relative humidity,wind speed,solar radiation,rainfall,atmospheric pressure,soil temperature,and soil humidity,were simultaneously measured in a grassland of Ergun City,Inner Mongolia Autonomous Region of China in 2021.We chose three regression models,i.e.,random forest(RF)model,extreme gradient boosting(XGB)model,and boosted regression tree(BRT)model,to model the seasonal DFMC according to the data collected.To ensure accuracy,we added time-lag variables of 3 d to the models.The results showed that the RF model had the best fitting effect with an R2value of 0.847 and a prediction accuracy with a mean absolute error score of 4.764%among the three models.The accuracies of the models in spring and autumn were higher than those in the other two seasons.In addition,different seasons had different key influencing factors,and the degree of influence of these factors on the DFMC changed with time lags.Moreover,time-lag variables within 44 h clearly improved the fitting effect and prediction accuracy,indicating that environmental conditions within approximately 48 h greatly influence the DFMC.This study highlights the importance of considering 48 h time-lagged variables when predicting the DFMC of grassland fuels and mapping grassland fire risks based on the DFMC to help locate high-priority areas for grassland fire monitoring and prevention.
基金Supported by Modern Agricultural Industry Technology System(CARS-45)Science and Technology Major Project of Hunan Province(2017NK1030).
文摘[Objectives]This study was conducted to evaluate the nutritional value and safety of black soldier flies fed with dead pig meat and bone meal(DPMBM)and to explore the resource utilization of DPMBM.[Methods]The general nutrient composition,amino acids,fatty acids and mineral elements of black soldier fly meal(BSLM)were detected and analyzed.[Results]The results showed that the contents of moisture,crude protein,crude fat and ash in BSLM were 3.42%,42.31%,34.04%and 5.40%,respectively.The contents of total amino acids(TAA),essential amino acids(∑EAA)and non-essential amino acids(∑NEAA)and umami amino acids(∑DAA),the EAA/TAA value and the EAA/NEAA value were 37.93%,13.08%,24.85%,13.43%,34.47%,52.61%,respectively.A total of eight kinds of saturated fatty acids and seven kinds of unsaturated fatty acids were detected,accounting for 63.65%and 32.67%of the total fatty acids.Among the major mineral elements,the content of Ca was the highest,followed by K,Mg and Na.Among the trace mineral elements,the content of Mn was the highest,followed by Fe,Zn,Cu,Ni,Cr,Cd,As,Pb,Se,Sn,Ti,Sb and Hg.The contents of heavy metal mineral elements in BSLM were far lower than the limits specified in Hygienical Standard for Feeds.[Conclusions]In conclusion,BSLM has high nutritional value and good safety of heavy metals,and thus great potential for development and utilization as a high quality dietary protein,fat and mineral elements source.
文摘Chest wall reconstruction is a surgical procedure aimed at restoring the integrity and function of the chest wall, which may be damaged due to trauma, cancer, infection, or congenital defects. The chest wall plays a vital role in protecting the thoracic organs, supporting the respiratory system, and maintaining the shape of the chest. Therefore, any defect or deformity of the chest wall can have significant functional and aesthetic consequences for the patient. The authors present a case report at Kenyatta National Hospital (KNH) of a dyspneic 47-year-old male patient with a right anterolateral chest wall defect post-pneumonectomy previously complicated by bronchopleural fistula. Past attempts at the chest wall reconstruction had utilized the ipsilateral latissimus dorsi muscle, pectoralis major muscle, and the omental pedicled flaps with limited success. A chimeric anterolateral thigh (ALT) perforator-free flap with vastus lateralis (VL) muscle was used to obliterate the post-pneumonectomy intrathoracic dead space and to provide a cutaneous paddle. This case report aims to show the versatility of the ALT flap for chest wall reconstruction to prevent the post-pneumonectomy syndrome associated with tracheal deviation, inspiratory stridor, and exertional dyspnea. In conclusion, chest wall reconstruction with obliteration of intrathoracic dead space post-pneumonectomy is challenging and needs careful planning and execution.
基金the European Structural and Investment Funded Grant"Cardio Metabolic"(#KK.01.2.1.02.0321)the Croatian National Centre of Research Excellence in Personalized Healthcare Grant(#KK.01.1.1.01.0010)+2 种基金the European Regional Development Fund Grant,project"CRISPR/Cas9-CasMouse"(#KK.01.1.1.04.0085)the European Structural and Investment Funded Project of Centre of Competence in Molecular Diagnostics(#KK.01.2.2.03.0006)the Croatian National Centre of Research Excellence in Personalized Healthcare Grant(#KK.01.1.1.01.0010).
文摘Hepatocyte nuclear factor 1 alpha(HNF1A),hepatocyte nuclear factor 4 alpha(HNF4A),and forkhead box protein A2(FOXA2)are key transcription factors that regulate a complex gene network in the liver,cre-ating a regulatory transcriptional loop.The Encode and ChIP-Atlas databases identify the recognition sites of these transcription factors in many glycosyltransferase genes.Our in silico analysis of HNF1A,HNF4A.and FOXA2 binding to the ten candidate glyco-genes studied in this work confirms a significant enrich-ment of these transcription factors specifically in the liver.Our previous studies identified HNF1A as a master regulator of fucosylation,glycan branching,and galactosylation of plasma glycoproteins.Here,we aimed to functionally validate the role of the three transcription factors on downstream glyco-gene transcriptional expression and the possible effect on glycan phenotype.We used the state-of-the-art clus-tered regularly interspaced short palindromic repeats/dead Cas9(CRISPR/dCas9)molecular tool for the downregulation of the HNF1A,HNF4A,and FOXA2 genes in HepG2 cells-a human liver cancer cell line.The results show that the downregulation of all three genes individually and in pairs affects the transcrip-tional activity of many glyco-genes,although downregulation of glyco-genes was not always followed by an unambiguous change in the corresponding glycan structures.The effect is better seen as an overall change in the total HepG2 N-glycome,primarily due to the extension of biantennary glycans.We propose an alternative way to evaluate the N-glycome composition via estimating the overall complexity of the glycome by quantifying the number of monomers in each glycan structure.We also propose a model showing feedback loops with the mutual activation of HNF1A-FOXA2 and HNF4A-FOXA2 affecting glyco-genes and protein glycosylation in HepG2 cells.
文摘The Jordan River, also referred to as Nahr Al Sharieat in Arabic, is a long river in the Middle East that flows from north to south through the Sea of Galilee to the Dead Sea. The Jordan River is bordered by the Golan Heights and the Hashemite Kingdom of Jordan in the east and by Israel and the Palestinian West Bank (Judea and Samaria) in the west. Soil tunnels, including those in the Jordan River watershed and on the Israel, Syria, and Lebanon borders, have a long history of use for warfare, as invasion pathways, smuggling, and storage of rockets, missiles and ordnance and are causes of serious political tension between the countries. Soil tunnel construction and destruction often has adverse environmental and human health impacts in the Jordan River landscape. Due to agricultural wastes, the discharge of untreated sewage, and diversion of saline springs into the river water there has been serious deterioration in the water quality in the lower courses of the Jordan River. The primary objective of this research is to encourage the development of a multi-country clean-up, mitigation, and protection plan for the Jordan River.
文摘The quantitative trait loci (QTLs) for the dead leaf rate (DLR) and the dead seedling rate (DSR) at the different rice growing periods after transplanting under alkaline stress were identified using an F2:3 population, which included 200 individuals and lines derived from a cross between two japonica rice cultivars Gaochan 106 and Changbai 9 with microsatellite markers. The DLR detected at 20 days to 62 days after transplanting under alkaline stress showed continuous normal or near normal distributions in F3 lines, which was the quantitative trait controlled by multiple genes. The DSR showed a continuous distribution with 3 or 4 peaks and was the quantitative trait controlled by main and multiple genes when rice was grown for 62 days after transplanting under alkaline stress. Thirteen QTLs associated with DLR were detected at 20 days to 62 days after transplanting under alkaline stress. Among these, qDLR9-2 located in RM5786-RM160 on chromosome 9 was detected at 34 days, 41 days, 48 days, 55 days, and 62 days, respectively; qDLR4 located in RM3524-RM3866 on chromosome 4 was detected at 34 days, 41 days, and 48 days, respectively; qDLR7-1 located in RM3859-RM320 on chromosome 7 was detected at 20 days and 27 days; and qDLR6-2 in RM1340-RM5957 on chromosome 6 was detected at 55 days and 62 days, respectively. The alleles of both qDLR9-2 and qDLR4 were derived from alkaline sensitive parent "Gaochanl06". The alleles of both qDLR7-1 and qDLR6-2 were from alkaline tolerant parent Changbai 9. These gene actions showed dominance and over dominance primarily. Six QTLs associated with DSR were detected at 62 days after transplanting under alkaline stress. Among these, qDSR6-2 and qDSR8 were located in RM1340-RM5957 on chromosome 6 and in RM3752-RM404 on chromosome 8, respectively, which were associated with DSR and accounted for 20.32% and 18.86% of the observed phenotypic variation, respectively; qDSR11-2 and qDSR11-3 were located in RM536-RM479 and RM2596-RM286 on chromosome 11, respectively, which were associated with DSR explaining 25.85% and 15.41% of the observed phenotypic variation, respectively. The marker flanking distances of these QTLs were quite far except that of qDSR6-2, which should be researched further.