[Objective]The aim was to isolate cellulose decomposing strains with high activity from straw-amended soil.[Method]Screening of CMC solid culture and shaking culture rescreening were carried out to obtain cellulose de...[Objective]The aim was to isolate cellulose decomposing strains with high activity from straw-amended soil.[Method]Screening of CMC solid culture and shaking culture rescreening were carried out to obtain cellulose decomposing strains with high activity from the corn straw amended soil,which were analyzed by 16rDNA sequence analysis.[Result]A bacterium and a fungus with higher endonucleases activity were obtained through preliminary screening and secondary screening,a fungus with higher filter paper enzyme activity,and a bacterium（NO.5 strain） with higher of both filter paper enzyme activity and CMC enzyme activity were obtained.The result of 16S rDNA sequence analysis showed that the similarity was 100% between NO.5 strain and Bacillus subtilis.[Conclusion]NO.5 strain was identified as Bacillus subtilis.展开更多
Cellulose decomposing fungi play an important role in litter decomposition and are decisive in nutrient cycling in sandy land ecosystems. Thirty-one strains were isolated to select efficient cellulose decomposers, and...Cellulose decomposing fungi play an important role in litter decomposition and are decisive in nutrient cycling in sandy land ecosystems. Thirty-one strains were isolated to select efficient cellulose decomposers, and four efficient cellulose decomposing fungi （NM3-1, NM3-2, NM3-3, and NM3-4） were screened using a CMC （carboxymethyl cellulose） carbon source in dune soil of Horqin Sandy Land. They were identified as Asperigillus calidoustus, Fusarium oxysporum, Fusarium solani, and Hypocrea lixii by rDNA-ITS molecular biological methods. Cloth decomposition rates were 15.71%, 15.89%, 17.29%, and 17.89% by the four efficient decomposers incubated for 30 days, respectively. Screening of efficient cellulose decomposers can not only increase the dune soil functional microbe bank, but can also accelerate litter decom- position and available nutrient input in the Horqin Sandy Land.展开更多
基金Supported by Natural Foundation of Education Department of Henan Province (2010B530001) Science and Technology Innovation Supporting Program Talents of University in Henan Province(2010HASTIT019)+1 种基金Key Technology Research and Demonstration of Large-scale Straw Gasification Equipment (092102310044)Key Technology Research and Demonstration of Large Cascade Straw Digestion Biogas Generating Installation (2009A180013)~~
文摘[Objective]The aim was to isolate cellulose decomposing strains with high activity from straw-amended soil.[Method]Screening of CMC solid culture and shaking culture rescreening were carried out to obtain cellulose decomposing strains with high activity from the corn straw amended soil,which were analyzed by 16rDNA sequence analysis.[Result]A bacterium and a fungus with higher endonucleases activity were obtained through preliminary screening and secondary screening,a fungus with higher filter paper enzyme activity,and a bacterium（NO.5 strain） with higher of both filter paper enzyme activity and CMC enzyme activity were obtained.The result of 16S rDNA sequence analysis showed that the similarity was 100% between NO.5 strain and Bacillus subtilis.[Conclusion]NO.5 strain was identified as Bacillus subtilis.
基金financially supported by the National Science and Technology Support Program (2011BAC07B02)National Natural Science Foundation of China (41401620 and 41171414)the Key Laboratory of Desert and Desertification Foundation (KLDD-2014-010) from Cold and Arid Regions Environmental and Engineering Research Institute,CAS
文摘Cellulose decomposing fungi play an important role in litter decomposition and are decisive in nutrient cycling in sandy land ecosystems. Thirty-one strains were isolated to select efficient cellulose decomposers, and four efficient cellulose decomposing fungi （NM3-1, NM3-2, NM3-3, and NM3-4） were screened using a CMC （carboxymethyl cellulose） carbon source in dune soil of Horqin Sandy Land. They were identified as Asperigillus calidoustus, Fusarium oxysporum, Fusarium solani, and Hypocrea lixii by rDNA-ITS molecular biological methods. Cloth decomposition rates were 15.71%, 15.89%, 17.29%, and 17.89% by the four efficient decomposers incubated for 30 days, respectively. Screening of efficient cellulose decomposers can not only increase the dune soil functional microbe bank, but can also accelerate litter decom- position and available nutrient input in the Horqin Sandy Land.
