There is an increasing interest in discovering new antibacterial agents derived from nature to enhance the treatment of various bacterial infections.Defensins and their derived peptide fragments exhibit significant an...There is an increasing interest in discovering new antibacterial agents derived from nature to enhance the treatment of various bacterial infections.Defensins and their derived peptide fragments exhibit significant antibacterial activity without any cytotoxic effects,making them attractive features for potential novel antibacterial therapeutics.Crassostrea gigas,a traditional seafood that has been used worldwide for centuries,has its shells applied in Chinese medicine as Ostreae concha.In this study,bioinformatics analysis was used to obtain a novel antibacterial peptide,CGD-1,derived from marine Chinese medicine Ostreae concha.The physicochemical characterization and circular dichroism analysis results demonstrated that CGD-1 assembled into anα-helical structure in a simulated membrane environment,and it displayed antibacterial action against Gram-negative bacteria.The minimal inhibitory concentrations against both Pseudomonas aeruginosa ATCC27853 and Escherichia coli ATCC25922 were 25μM.CGD-1 was able to efficiently permeate the cell membrane.Changes in bacterial cell morphology were evaluated using a field emission scanning electron microscope.The results suggested that CGD-1 exerted its antibacterial activity through permeabilizing and disrupting the bacterial cell membrane.Therefore,CGD-1 may have potential applications in fighting against pathogenic bacteria such as P.aeruginosa and E.coli.展开更多
为了探究公羊睾丸和附睾不同区段β防御素124(beta-defensins 124,BD124)的表达特征与精液品质之间的关系,该研究选取20只健康杜泊羊×湖羊杂交种公羊,采集新鲜精液测定精子运动学参数,根据精子活力(sperm motility,SM)将公羊分为...为了探究公羊睾丸和附睾不同区段β防御素124(beta-defensins 124,BD124)的表达特征与精液品质之间的关系,该研究选取20只健康杜泊羊×湖羊杂交种公羊,采集新鲜精液测定精子运动学参数,根据精子活力(sperm motility,SM)将公羊分为高品质组(SM≥60%)和低品质组(SM≤15%),每组各选取3只采集睾丸和附睾不同区段组织,通过免疫组织化学法和Western blot检测BD124定位及表达。结果表明:(1)BD124在附睾头(epididymis head,EH1~EH2)平均光密度值和相对灰度值极显著高于附睾体(epididymis body,EB1~EB3)和附睾尾(epididymis tail,ET1~ET2)(P<0.01),高品质组输出小管(efferent ducts,ED)、EH2、EB1、EB3和ET2区段BD124平均光密度值和相对灰度值均极显著高于低品质组(P<0.01);(2)高品质组精子活率(sperm viability,SV)、A级精子比例(percentage of spermatozoa of the A class,A percent)、平均路径速度(average path velocity,VAP)、直线速度(straight line velocity,VSL)、曲线速度(curvilinear velocity,VCL)和平均侧摆幅值(amplitude of lateral head displacement,ALH)均极显著高于低品质组(P<0.01);(3)相关性分析显示EB1、ET2区段BD124相对灰度值和平均光密度值与SV呈极显著正相关(P<0.01),EB1、ET2区段BD124相对灰度值和平均光密度值与D级精子比例呈极显著负相关(P<0.01),ED、EH2、EB1、EB3、ET2区段BD124的相对灰度值和平均光密度值与A级精子比例、VAP、VSL、VCL、ALH呈极显著正相关(P<0.01)。由此可知,EB1和ET2区段BD124表达与公羊精液品质呈明显正相关关系。展开更多
基金This research work was financially supported National Natural Science Foundation of China(No.82003910)Guangdong Basic and Applied Basic Research Foundation(2020A1515110550)+2 种基金Funding Key R&D Program of Shandong Province(2022SFGC0105)Exploration Innovation Team(2021GXRC062),Jinan Talent Project for Universities(202228088)Key innovation Project of Qilu University of Technology(Shandong Academy of Sciences)(2022JBZ01-06).
文摘There is an increasing interest in discovering new antibacterial agents derived from nature to enhance the treatment of various bacterial infections.Defensins and their derived peptide fragments exhibit significant antibacterial activity without any cytotoxic effects,making them attractive features for potential novel antibacterial therapeutics.Crassostrea gigas,a traditional seafood that has been used worldwide for centuries,has its shells applied in Chinese medicine as Ostreae concha.In this study,bioinformatics analysis was used to obtain a novel antibacterial peptide,CGD-1,derived from marine Chinese medicine Ostreae concha.The physicochemical characterization and circular dichroism analysis results demonstrated that CGD-1 assembled into anα-helical structure in a simulated membrane environment,and it displayed antibacterial action against Gram-negative bacteria.The minimal inhibitory concentrations against both Pseudomonas aeruginosa ATCC27853 and Escherichia coli ATCC25922 were 25μM.CGD-1 was able to efficiently permeate the cell membrane.Changes in bacterial cell morphology were evaluated using a field emission scanning electron microscope.The results suggested that CGD-1 exerted its antibacterial activity through permeabilizing and disrupting the bacterial cell membrane.Therefore,CGD-1 may have potential applications in fighting against pathogenic bacteria such as P.aeruginosa and E.coli.
文摘为了探究公羊睾丸和附睾不同区段β防御素124(beta-defensins 124,BD124)的表达特征与精液品质之间的关系,该研究选取20只健康杜泊羊×湖羊杂交种公羊,采集新鲜精液测定精子运动学参数,根据精子活力(sperm motility,SM)将公羊分为高品质组(SM≥60%)和低品质组(SM≤15%),每组各选取3只采集睾丸和附睾不同区段组织,通过免疫组织化学法和Western blot检测BD124定位及表达。结果表明:(1)BD124在附睾头(epididymis head,EH1~EH2)平均光密度值和相对灰度值极显著高于附睾体(epididymis body,EB1~EB3)和附睾尾(epididymis tail,ET1~ET2)(P<0.01),高品质组输出小管(efferent ducts,ED)、EH2、EB1、EB3和ET2区段BD124平均光密度值和相对灰度值均极显著高于低品质组(P<0.01);(2)高品质组精子活率(sperm viability,SV)、A级精子比例(percentage of spermatozoa of the A class,A percent)、平均路径速度(average path velocity,VAP)、直线速度(straight line velocity,VSL)、曲线速度(curvilinear velocity,VCL)和平均侧摆幅值(amplitude of lateral head displacement,ALH)均极显著高于低品质组(P<0.01);(3)相关性分析显示EB1、ET2区段BD124相对灰度值和平均光密度值与SV呈极显著正相关(P<0.01),EB1、ET2区段BD124相对灰度值和平均光密度值与D级精子比例呈极显著负相关(P<0.01),ED、EH2、EB1、EB3、ET2区段BD124的相对灰度值和平均光密度值与A级精子比例、VAP、VSL、VCL、ALH呈极显著正相关(P<0.01)。由此可知,EB1和ET2区段BD124表达与公羊精液品质呈明显正相关关系。
